Collinsella aerofaciens
Taxonomy:
Average proteome isoelectric point is 5.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1812 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A173XC82|A0A173XC82_9ACTN Phosphatase YidA OS=Collinsella aerofaciens OX=74426 GN=yidA_2 PE=4 SV=1
MM1 pKa = 7.31 SKK3 pKa = 9.97 IAVVYY8 pKa = 9.68 WSGTGNTEE16 pKa = 3.81 AMANLVAEE24 pKa = 4.88 GATDD28 pKa = 3.69 AGAEE32 pKa = 4.13 AEE34 pKa = 4.69 VISCADD40 pKa = 4.0 FSADD44 pKa = 3.46 KK45 pKa = 10.86 AAGYY49 pKa = 9.84 DD50 pKa = 4.11 AIAFGCPAMGSEE62 pKa = 4.0 EE63 pKa = 4.38 LEE65 pKa = 3.95 YY66 pKa = 11.37 DD67 pKa = 3.66 EE68 pKa = 5.46 FQPMWDD74 pKa = 3.62 EE75 pKa = 4.28 VKK77 pKa = 9.66 EE78 pKa = 4.1 TLGDD82 pKa = 3.62 KK83 pKa = 10.6 KK84 pKa = 11.11 VVLFGSYY91 pKa = 7.96 SWAEE95 pKa = 4.16 GEE97 pKa = 5.68 WMDD100 pKa = 4.83 NWKK103 pKa = 10.67 ADD105 pKa = 3.6 ADD107 pKa = 4.07 EE108 pKa = 4.9 EE109 pKa = 4.78 GVNVVDD115 pKa = 6.34 SVICYY120 pKa = 8.04 DD121 pKa = 3.93 APDD124 pKa = 4.83 DD125 pKa = 4.15 EE126 pKa = 6.12 SEE128 pKa = 4.18 AACRR132 pKa = 11.84 ALGAEE137 pKa = 4.42 LAA139 pKa = 4.23
Molecular weight: 14.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.758
IPC2_protein 3.63
IPC_protein 3.605
Toseland 3.401
ProMoST 3.757
Dawson 3.579
Bjellqvist 3.745
Wikipedia 3.503
Rodwell 3.439
Grimsley 3.312
Solomon 3.567
Lehninger 3.516
Nozaki 3.706
DTASelect 3.872
Thurlkill 3.452
EMBOSS 3.516
Sillero 3.719
Patrickios 0.896
IPC_peptide 3.567
IPC2_peptide 3.694
IPC2.peptide.svr19 3.682
Protein with the highest isoelectric point:
>tr|A0A174C3R5|A0A174C3R5_9ACTN Arginine transport ATP-binding protein ArtM OS=Collinsella aerofaciens OX=74426 GN=artM_2 PE=4 SV=1
MM1 pKa = 7.85 RR2 pKa = 11.84 LGGISGTGNALVRR15 pKa = 11.84 RR16 pKa = 11.84 LAVEE20 pKa = 4.65 GSWCYY25 pKa = 10.66 AQAGHH30 pKa = 5.84 QPKK33 pKa = 10.45 LMPKK37 pKa = 9.76 GSGVPLEE44 pKa = 4.21 VRR46 pKa = 11.84 MHH48 pKa = 5.47 GRR50 pKa = 11.84 KK51 pKa = 9.77 GSEE54 pKa = 3.34 RR55 pKa = 11.84 LLRR58 pKa = 11.84 RR59 pKa = 11.84 RR60 pKa = 11.84 EE61 pKa = 3.86 EE62 pKa = 3.76 MLEE65 pKa = 3.9 RR66 pKa = 11.84 GMPQAKK72 pKa = 10.1 ANAATAAEE80 pKa = 4.21 LVRR83 pKa = 11.84 WLWALGTMCRR93 pKa = 11.84 EE94 pKa = 4.0 ATEE97 pKa = 3.93
Molecular weight: 10.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.428
IPC_protein 10.131
Toseland 10.687
ProMoST 10.365
Dawson 10.745
Bjellqvist 10.467
Wikipedia 10.965
Rodwell 10.891
Grimsley 10.774
Solomon 10.906
Lehninger 10.877
Nozaki 10.687
DTASelect 10.452
Thurlkill 10.672
EMBOSS 11.096
Sillero 10.687
Patrickios 10.687
IPC_peptide 10.921
IPC2_peptide 9.648
IPC2.peptide.svr19 8.657
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1812
0
1812
611423
29
2395
337.4
36.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.408 ± 0.083
1.619 ± 0.024
6.463 ± 0.045
6.551 ± 0.062
3.645 ± 0.041
8.035 ± 0.045
2.013 ± 0.024
5.392 ± 0.046
4.062 ± 0.047
9.008 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.74 ± 0.028
3.071 ± 0.031
4.226 ± 0.041
2.997 ± 0.034
5.783 ± 0.068
5.734 ± 0.047
5.512 ± 0.042
7.965 ± 0.053
0.975 ± 0.021
2.799 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here