Avian paramyxovirus goose/Shimane/67/2000
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A173M8Y4|A0A173M8Y4_9MONO Phosphoprotein OS=Avian paramyxovirus goose/Shimane/67/2000 OX=1401445 GN=P PE=4 SV=1
MM1 pKa = 7.54 ARR3 pKa = 11.84 FSQEE7 pKa = 3.2 IFGLSTILLIAQTCQGSIDD26 pKa = 3.72 GRR28 pKa = 11.84 LTLAAGIVPVGDD40 pKa = 3.84 RR41 pKa = 11.84 PISIYY46 pKa = 10.3 TSSQTGIIVVKK57 pKa = 9.71 LIPNLPDD64 pKa = 3.3 NKK66 pKa = 10.0 KK67 pKa = 10.58 DD68 pKa = 3.57 CAKK71 pKa = 10.62 QSLQSYY77 pKa = 10.07 NEE79 pKa = 3.98 TLSRR83 pKa = 11.84 ILTPLATAMSAIRR96 pKa = 11.84 GNSTTQVRR104 pKa = 11.84 EE105 pKa = 3.7 NRR107 pKa = 11.84 LVGAIIGSVALGVATAAQITAATALIQANQNAANIARR144 pKa = 11.84 LANSIAKK151 pKa = 8.97 TNEE154 pKa = 3.37 AVTDD158 pKa = 3.83 LTEE161 pKa = 4.37 GLGTLAIGVGKK172 pKa = 9.68 LQDD175 pKa = 3.65 YY176 pKa = 11.19 VNEE179 pKa = 4.23 QFNNTAVAIDD189 pKa = 4.04 CLTLEE194 pKa = 4.32 SRR196 pKa = 11.84 LGIQLSLYY204 pKa = 8.73 LTEE207 pKa = 4.34 LMGVFGNQLTSPALTPITIQALYY230 pKa = 10.45 NLAGGNLNALLSRR243 pKa = 11.84 LGASEE248 pKa = 4.21 TQLGSLINSGLIKK261 pKa = 10.44 GMPIMYY267 pKa = 10.24 DD268 pKa = 3.42 DD269 pKa = 4.49 ANKK272 pKa = 10.36 LLAVQVEE279 pKa = 4.44 LPSIGKK285 pKa = 9.81 LNGARR290 pKa = 11.84 STLLEE295 pKa = 4.14 TLAVDD300 pKa = 4.06 TTRR303 pKa = 11.84 GPSSPIIPSAVIEE316 pKa = 4.24 IGGAMEE322 pKa = 4.79 EE323 pKa = 4.84 LDD325 pKa = 4.8 LSPCITTDD333 pKa = 4.04 LDD335 pKa = 3.8 MFCTKK340 pKa = 10.22 IISYY344 pKa = 9.39 PLSQSTLSCLNGNLSDD360 pKa = 4.19 CVFSRR365 pKa = 11.84 SEE367 pKa = 4.29 GVLSTPYY374 pKa = 8.38 MTIKK378 pKa = 10.66 GKK380 pKa = 10.15 IVANCKK386 pKa = 8.89 QVICRR391 pKa = 11.84 CMDD394 pKa = 3.97 PPQILSQNYY403 pKa = 9.37 GEE405 pKa = 4.89 ALLLIDD411 pKa = 4.95 EE412 pKa = 4.62 STCRR416 pKa = 11.84 SLEE419 pKa = 3.88 LSGVILKK426 pKa = 10.35 LAGTYY431 pKa = 8.9 EE432 pKa = 4.1 SEE434 pKa = 4.21 YY435 pKa = 10.47 TRR437 pKa = 11.84 NLTVDD442 pKa = 4.12 PSQVIITGPLDD453 pKa = 3.26 ISAEE457 pKa = 3.98 LSKK460 pKa = 11.13 VNQSIDD466 pKa = 3.48 SAKK469 pKa = 10.85 EE470 pKa = 3.57 NIAEE474 pKa = 4.3 SNKK477 pKa = 9.86 FLSQVNVKK485 pKa = 9.87 LLSSSAMITYY495 pKa = 9.9 IVATVVCLIIAITGCVIGIYY515 pKa = 9.81 TLTKK519 pKa = 10.43 LKK521 pKa = 10.53 SQQEE525 pKa = 4.22 TLLWLGNNAEE535 pKa = 4.31 MHH537 pKa = 6.49 GSRR540 pKa = 11.84 SKK542 pKa = 10.13 TSFF545 pKa = 2.94
Molecular weight: 57.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.031
IPC2_protein 5.207
IPC_protein 5.105
Toseland 4.965
ProMoST 5.194
Dawson 5.041
Bjellqvist 5.194
Wikipedia 4.889
Rodwell 4.952
Grimsley 4.876
Solomon 5.041
Lehninger 4.991
Nozaki 5.156
DTASelect 5.283
Thurlkill 4.965
EMBOSS 4.914
Sillero 5.219
Patrickios 3.808
IPC_peptide 5.054
IPC2_peptide 5.219
IPC2.peptide.svr19 5.252
Protein with the highest isoelectric point:
>tr|A0A173M8X5|A0A173M8X5_9MONO Hemagglutinin-neuraminidase OS=Avian paramyxovirus goose/Shimane/67/2000 OX=1401445 GN=HN PE=3 SV=1
MM1 pKa = 8.06 DD2 pKa = 4.14 SSKK5 pKa = 10.76 IIGLEE10 pKa = 3.76 VDD12 pKa = 3.97 PSSPSNTLLAFPVVLQEE29 pKa = 3.67 IDD31 pKa = 3.31 GGRR34 pKa = 11.84 KK35 pKa = 9.37 EE36 pKa = 3.83 ITPQFRR42 pKa = 11.84 TQKK45 pKa = 9.73 IDD47 pKa = 2.81 MWSEE51 pKa = 4.16 SKK53 pKa = 10.64 SDD55 pKa = 3.54 SVSITTYY62 pKa = 10.55 GFIYY66 pKa = 9.87 GVKK69 pKa = 10.14 GGNDD73 pKa = 2.97 NSGPIMAEE81 pKa = 3.8 QQKK84 pKa = 9.87 EE85 pKa = 4.11 PLSAAMLCFGSVGYY99 pKa = 10.64 NSGLPEE105 pKa = 3.83 IARR108 pKa = 11.84 AALNTIITCKK118 pKa = 10.37 KK119 pKa = 9.7 SATDD123 pKa = 3.3 SEE125 pKa = 5.04 RR126 pKa = 11.84 ILFTVHH132 pKa = 6.16 QAPQMIQEE140 pKa = 4.51 AKK142 pKa = 10.4 VISTRR147 pKa = 11.84 YY148 pKa = 10.02 SSVAANKK155 pKa = 9.81 CVRR158 pKa = 11.84 APEE161 pKa = 4.19 RR162 pKa = 11.84 VPSGLSLEE170 pKa = 4.41 YY171 pKa = 10.53 KK172 pKa = 9.15 VTFVSLTVVPKK183 pKa = 10.13 SDD185 pKa = 3.36 VYY187 pKa = 10.77 KK188 pKa = 10.69 VPRR191 pKa = 11.84 PVLRR195 pKa = 11.84 LHH197 pKa = 6.54 SKK199 pKa = 10.12 HH200 pKa = 6.16 VLNIAINAVIQIDD213 pKa = 3.67 IQATHH218 pKa = 7.39 PLAKK222 pKa = 9.84 TLIKK226 pKa = 10.91 RR227 pKa = 11.84 NDD229 pKa = 3.29 QFFADD234 pKa = 4.46 LFIHH238 pKa = 6.99 IGMISCLDD246 pKa = 3.51 SKK248 pKa = 11.23 GNKK251 pKa = 9.14 ISVDD255 pKa = 3.22 KK256 pKa = 11.49 LEE258 pKa = 4.15 LKK260 pKa = 9.67 IRR262 pKa = 11.84 RR263 pKa = 11.84 MSISVGLLDD272 pKa = 4.76 IFGPSIALKK281 pKa = 10.78 ARR283 pKa = 11.84 GKK285 pKa = 8.16 RR286 pKa = 11.84 TKK288 pKa = 10.64 VMSPFFSPRR297 pKa = 11.84 GTACYY302 pKa = 9.21 PISQTAPGIAKK313 pKa = 9.82 ILWSQTGSLHH323 pKa = 5.01 EE324 pKa = 4.57 CKK326 pKa = 10.24 IIIQGGTNRR335 pKa = 11.84 AIATTDD341 pKa = 3.4 DD342 pKa = 4.02 FVVGSTKK349 pKa = 9.67 IEE351 pKa = 3.77 KK352 pKa = 10.06 SGRR355 pKa = 11.84 NGKK358 pKa = 9.62 FNPFKK363 pKa = 10.55 KK364 pKa = 9.16 TGG366 pKa = 3.25
Molecular weight: 39.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.929
IPC2_protein 8.931
IPC_protein 8.814
Toseland 9.809
ProMoST 9.399
Dawson 9.984
Bjellqvist 9.633
Wikipedia 10.087
Rodwell 10.555
Grimsley 10.014
Solomon 9.999
Lehninger 9.984
Nozaki 9.882
DTASelect 9.589
Thurlkill 9.853
EMBOSS 10.204
Sillero 9.911
Patrickios 10.16
IPC_peptide 10.014
IPC2_peptide 8.185
IPC2.peptide.svr19 7.827
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
4610
366
2199
768.3
84.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.028 ± 0.719
1.931 ± 0.228
5.293 ± 0.313
5.054 ± 0.214
3.427 ± 0.456
6.334 ± 0.527
1.8 ± 0.389
8.156 ± 0.543
5.184 ± 0.486
10.087 ± 0.938
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.777 ± 0.282
4.989 ± 0.239
4.382 ± 0.433
4.121 ± 0.252
4.772 ± 0.393
9.111 ± 0.825
6.226 ± 0.541
5.466 ± 0.276
0.868 ± 0.152
2.993 ± 0.32
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here