Gull circovirus
Average proteome isoelectric point is 8.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I3QHF3|I3QHF3_9CIRC C2 OS=Gull circovirus OX=400121 GN=C2 PE=4 SV=1
MM1 pKa = 8.0 RR2 pKa = 11.84 SRR4 pKa = 11.84 PRR6 pKa = 11.84 SGRR9 pKa = 11.84 YY10 pKa = 8.91 LRR12 pKa = 11.84 SIMAARR18 pKa = 11.84 RR19 pKa = 11.84 DD20 pKa = 3.41 SGARR24 pKa = 11.84 RR25 pKa = 11.84 WCFTLNNYY33 pKa = 7.86 TPEE36 pKa = 4.12 EE37 pKa = 4.22 EE38 pKa = 3.89 EE39 pKa = 3.92 TARR42 pKa = 11.84 NLTHH46 pKa = 6.75 EE47 pKa = 4.23 ADD49 pKa = 3.68 KK50 pKa = 11.3 YY51 pKa = 11.01 AFAIIGKK58 pKa = 8.64 EE59 pKa = 4.02 VGEE62 pKa = 4.5 SGTPHH67 pKa = 6.28 LQGFLHH73 pKa = 6.86 FKK75 pKa = 9.83 QKK77 pKa = 9.98 QRR79 pKa = 11.84 LTALKK84 pKa = 10.24 KK85 pKa = 10.18 LFPRR89 pKa = 11.84 AHH91 pKa = 6.26 FEE93 pKa = 3.84 KK94 pKa = 10.81 ARR96 pKa = 11.84 GSDD99 pKa = 3.4 QQNADD104 pKa = 3.37 YY105 pKa = 10.52 CGKK108 pKa = 10.36 DD109 pKa = 3.54 GEE111 pKa = 4.53 ILTMIGTPSDD121 pKa = 3.74 NKK123 pKa = 10.62 PSDD126 pKa = 3.44 LAGAVAAVKK135 pKa = 10.29 RR136 pKa = 11.84 GSQMSEE142 pKa = 3.23 IARR145 pKa = 11.84 EE146 pKa = 3.8 FSEE149 pKa = 5.75 VYY151 pKa = 10.0 VKK153 pKa = 10.63 YY154 pKa = 10.91 GRR156 pKa = 11.84 GLRR159 pKa = 11.84 DD160 pKa = 3.3 LRR162 pKa = 11.84 LLIGCPPRR170 pKa = 11.84 DD171 pKa = 3.66 FKK173 pKa = 11.36 TEE175 pKa = 4.08 VIVLIGPPGCGKK187 pKa = 10.54 SKK189 pKa = 10.32 LANEE193 pKa = 4.27 MEE195 pKa = 4.17 GSKK198 pKa = 10.1 FYY200 pKa = 11.27 KK201 pKa = 9.95 MKK203 pKa = 10.67 GDD205 pKa = 2.88 WWDD208 pKa = 4.09 GYY210 pKa = 11.18 DD211 pKa = 3.56 NQDD214 pKa = 3.06 IVIIDD219 pKa = 4.59 DD220 pKa = 4.42 FYY222 pKa = 11.85 GWLPYY227 pKa = 10.16 CEE229 pKa = 4.67 CLRR232 pKa = 11.84 LCDD235 pKa = 4.47 RR236 pKa = 11.84 YY237 pKa = 9.45 PHH239 pKa = 6.33 RR240 pKa = 11.84 VPVKK244 pKa = 8.48 GAYY247 pKa = 9.67 VEE249 pKa = 4.44 FTSKK253 pKa = 10.81 KK254 pKa = 9.99 IVFTSNRR261 pKa = 11.84 HH262 pKa = 4.16 VDD264 pKa = 3.69 GWWKK268 pKa = 11.27 GEE270 pKa = 3.89 IEE272 pKa = 3.88 KK273 pKa = 10.74 SAFYY277 pKa = 10.75 RR278 pKa = 11.84 RR279 pKa = 11.84 INVYY283 pKa = 10.1 KK284 pKa = 10.35 FYY286 pKa = 10.2 EE287 pKa = 4.1 TGEE290 pKa = 4.21 FKK292 pKa = 11.1 DD293 pKa = 4.07 MPGHH297 pKa = 6.05 MLPHH301 pKa = 7.35 PINYY305 pKa = 9.73
Molecular weight: 35.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.52
IPC2_protein 8.434
IPC_protein 8.331
Toseland 8.785
ProMoST 8.887
Dawson 9.194
Bjellqvist 9.151
Wikipedia 9.326
Rodwell 9.311
Grimsley 9.151
Solomon 9.268
Lehninger 9.224
Nozaki 9.253
DTASelect 9.019
Thurlkill 9.107
EMBOSS 9.326
Sillero 9.297
Patrickios 4.52
IPC_peptide 9.253
IPC2_peptide 7.922
IPC2.peptide.svr19 7.725
Protein with the highest isoelectric point:
>tr|Q06J76|Q06J76_9CIRC Capsid protein OS=Gull circovirus OX=400121 GN=cap PE=3 SV=1
MM1 pKa = 7.71 RR2 pKa = 11.84 RR3 pKa = 11.84 RR4 pKa = 11.84 HH5 pKa = 5.66 RR6 pKa = 11.84 LPRR9 pKa = 11.84 NRR11 pKa = 11.84 RR12 pKa = 11.84 GHH14 pKa = 5.84 RR15 pKa = 11.84 LNRR18 pKa = 11.84 IYY20 pKa = 10.98 LFRR23 pKa = 11.84 FRR25 pKa = 11.84 RR26 pKa = 11.84 QFTVDD31 pKa = 4.56 FPSKK35 pKa = 9.79 TDD37 pKa = 3.55 PQKK40 pKa = 9.5 WASDD44 pKa = 3.39 FFTFNLNDD52 pKa = 4.55 FIGQEE57 pKa = 4.57 GIPAAWPFEE66 pKa = 4.3 DD67 pKa = 4.89 YY68 pKa = 10.58 RR69 pKa = 11.84 INLAKK74 pKa = 10.46 VVLRR78 pKa = 11.84 PEE80 pKa = 4.59 GVTTTIARR88 pKa = 11.84 GWGYY92 pKa = 7.4 TVPVQDD98 pKa = 5.56 ARR100 pKa = 11.84 VKK102 pKa = 10.47 DD103 pKa = 4.51 LKK105 pKa = 11.2 LQDD108 pKa = 3.5 QTQDD112 pKa = 3.68 PLANWDD118 pKa = 3.75 GARR121 pKa = 11.84 AWNLVRR127 pKa = 11.84 GFKK130 pKa = 10.47 RR131 pKa = 11.84 LVRR134 pKa = 11.84 PKK136 pKa = 9.85 PQLTISDD143 pKa = 3.65 MTASNFSASMWLNSSRR159 pKa = 11.84 SGWLPLQMPAGTQRR173 pKa = 11.84 DD174 pKa = 4.06 GTRR177 pKa = 11.84 VVHH180 pKa = 6.24 YY181 pKa = 9.9 GLAFSWPSPGQALRR195 pKa = 11.84 YY196 pKa = 8.5 IAEE199 pKa = 3.71 ITIYY203 pKa = 9.55 VTFRR207 pKa = 11.84 QFAQIMLKK215 pKa = 10.36 KK216 pKa = 10.41 SEE218 pKa = 4.18 EE219 pKa = 4.12 DD220 pKa = 3.04 LSKK223 pKa = 11.14 FGDD226 pKa = 4.14 CLTITDD232 pKa = 4.19 NEE234 pKa = 4.37 KK235 pKa = 11.0 DD236 pKa = 3.16 IDD238 pKa = 4.01 WNVVGSLL245 pKa = 3.67
Molecular weight: 28.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.256
IPC2_protein 9.414
IPC_protein 9.823
Toseland 10.306
ProMoST 9.97
Dawson 10.452
Bjellqvist 10.131
Wikipedia 10.628
Rodwell 10.716
Grimsley 10.511
Solomon 10.511
Lehninger 10.482
Nozaki 10.277
DTASelect 10.131
Thurlkill 10.321
EMBOSS 10.701
Sillero 10.365
Patrickios 10.394
IPC_peptide 10.511
IPC2_peptide 8.814
IPC2.peptide.svr19 8.641
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
689
139
305
229.7
26.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.096 ± 0.307
1.451 ± 0.493
6.096 ± 0.393
5.08 ± 1.053
5.515 ± 0.326
7.402 ± 0.775
2.758 ± 0.907
5.225 ± 0.171
5.66 ± 1.219
8.273 ± 1.087
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.322 ± 0.162
3.774 ± 0.317
5.37 ± 0.15
3.338 ± 0.836
9.144 ± 0.531
5.806 ± 0.42
5.225 ± 0.703
5.806 ± 0.872
2.322 ± 0.618
3.338 ± 1.073
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here