Mariniphaga sediminis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Marinilabiliales; Prolixibacteraceae; Mariniphaga

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4517 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A399D6A9|A0A399D6A9_9BACT Dipeptidase OS=Mariniphaga sediminis OX=1628158 GN=D1164_00970 PE=3 SV=1
MM1 pKa = 7.62RR2 pKa = 11.84KK3 pKa = 9.36IYY5 pKa = 10.62LLLLIMIYY13 pKa = 10.48SSALMAQDD21 pKa = 3.9CPGDD25 pKa = 3.26IVVNNDD31 pKa = 3.41PGICGAVVDD40 pKa = 4.67YY41 pKa = 10.36AAPSPAGATVTQIDD55 pKa = 4.19EE56 pKa = 4.47SGLTSGDD63 pKa = 3.43QFPVGEE69 pKa = 4.2TLQEE73 pKa = 3.98YY74 pKa = 10.13EE75 pKa = 4.21LDD77 pKa = 3.7YY78 pKa = 11.62GIGGKK83 pKa = 7.58DD84 pKa = 2.9TCSFTVTVNDD94 pKa = 3.74TEE96 pKa = 5.02APLLSCQNISVNNDD110 pKa = 3.14PGEE113 pKa = 4.4AGAEE117 pKa = 4.03VTIPVPYY124 pKa = 9.94HH125 pKa = 5.92NNPAVTEE132 pKa = 4.29TVDD135 pKa = 4.21VLLLYY140 pKa = 10.67ADD142 pKa = 4.13YY143 pKa = 11.08DD144 pKa = 4.07SYY146 pKa = 11.97AQDD149 pKa = 3.14VKK151 pKa = 11.47AKK153 pKa = 10.72LLGTGNFSTVHH164 pKa = 5.83TFNISSRR171 pKa = 11.84VPEE174 pKa = 4.2LEE176 pKa = 3.87GLLEE180 pKa = 4.32FDD182 pKa = 6.01VIMLWRR188 pKa = 11.84NGSFPDD194 pKa = 4.11PEE196 pKa = 4.87ALGNVLAGFVDD207 pKa = 3.95AGRR210 pKa = 11.84GLVMAGNALSDD221 pKa = 3.19ISYY224 pKa = 10.14LAGAFEE230 pKa = 4.08TDD232 pKa = 3.34EE233 pKa = 4.54YY234 pKa = 11.51GVIVPGYY241 pKa = 8.49IQYY244 pKa = 10.81GGQAYY249 pKa = 10.47LGDD252 pKa = 3.91VHH254 pKa = 6.47LTSHH258 pKa = 7.34PLMNSITSFDD268 pKa = 3.88GGLLSYY274 pKa = 10.69RR275 pKa = 11.84SISNAITPGSYY286 pKa = 9.26RR287 pKa = 11.84VADD290 pKa = 3.63WTDD293 pKa = 3.47GNPLIAAKK301 pKa = 10.41EE302 pKa = 3.9NVGASNARR310 pKa = 11.84RR311 pKa = 11.84VDD313 pKa = 3.98LNFFPPTVTDD323 pKa = 3.56HH324 pKa = 6.9GRR326 pKa = 11.84VPWDD330 pKa = 3.32VNTDD334 pKa = 2.88GDD336 pKa = 5.1LIMANAILWVAGAPIAYY353 pKa = 8.45DD354 pKa = 2.98NCGPVEE360 pKa = 4.05FSNNFNASTSPSGYY374 pKa = 9.97YY375 pKa = 9.93PIGSTSVTLNATDD388 pKa = 3.62EE389 pKa = 4.55AGNTSTCNFTVTVEE403 pKa = 5.21DD404 pKa = 3.7IDD406 pKa = 4.37PPVAICKK413 pKa = 10.07NISVVLDD420 pKa = 3.52EE421 pKa = 4.98NGQVTIEE428 pKa = 4.25GSDD431 pKa = 3.53VDD433 pKa = 5.24GGSTDD438 pKa = 2.98NHH440 pKa = 6.77AIATYY445 pKa = 10.44EE446 pKa = 4.11LDD448 pKa = 3.52TNTFDD453 pKa = 6.54DD454 pKa = 4.84SMLGEE459 pKa = 4.22NTVVLTVIDD468 pKa = 3.81EE469 pKa = 4.75AGNSSTCTATVQVFDD484 pKa = 4.88NNPPDD489 pKa = 4.07IVVQPIEE496 pKa = 4.07VALDD500 pKa = 3.24NTGNYY505 pKa = 9.31EE506 pKa = 4.3LTLEE510 pKa = 4.59DD511 pKa = 4.28LKK513 pKa = 11.6EE514 pKa = 4.08MAEE517 pKa = 4.2GTTDD521 pKa = 3.25NGTSFDD527 pKa = 3.84NLKK530 pKa = 10.64FSAFPTTFACEE541 pKa = 4.11NVGEE545 pKa = 4.41EE546 pKa = 3.94INVRR550 pKa = 11.84LTVEE554 pKa = 4.33DD555 pKa = 3.95EE556 pKa = 4.49SGNSDD561 pKa = 3.73KK562 pKa = 11.02KK563 pKa = 7.79WTSVTVVDD571 pKa = 5.53IIPPTVVCQNIEE583 pKa = 3.98VYY585 pKa = 10.82LDD587 pKa = 3.6EE588 pKa = 5.64NGEE591 pKa = 3.91ASITPEE597 pKa = 4.27EE598 pKa = 4.34INVSGEE604 pKa = 3.77GGSYY608 pKa = 9.76DD609 pKa = 3.24ACGIASLEE617 pKa = 4.03LDD619 pKa = 3.52KK620 pKa = 11.06MAFGCEE626 pKa = 3.82DD627 pKa = 3.11TGEE630 pKa = 4.28NNVVLSVTDD639 pKa = 3.34HH640 pKa = 6.46GGNTGSCTAIVTVKK654 pKa = 10.07DD655 pKa = 3.23TFPPVFEE662 pKa = 4.26PVADD666 pKa = 3.01IVVEE670 pKa = 4.1VEE672 pKa = 4.11TNITEE677 pKa = 4.27TTIDD681 pKa = 3.58YY682 pKa = 10.16PNIVVTDD689 pKa = 3.71NCSVTLEE696 pKa = 4.21LTEE699 pKa = 4.26GLGPDD704 pKa = 3.48GVFPVGTTTEE714 pKa = 3.67TWAASDD720 pKa = 3.63PAGNSMTLTFTVTVTPVNSPPVLVNPIADD749 pKa = 3.51QAVNASYY756 pKa = 10.86VLKK759 pKa = 10.95VPVRR763 pKa = 11.84PEE765 pKa = 3.67LGEE768 pKa = 4.07VFDD771 pKa = 6.16DD772 pKa = 4.06VDD774 pKa = 4.05GDD776 pKa = 4.02EE777 pKa = 4.44LTISAMLEE785 pKa = 4.01NGDD788 pKa = 5.03PLPAWAEE795 pKa = 4.05MVDD798 pKa = 3.83DD799 pKa = 4.55SLVFTPLIADD809 pKa = 4.3TGCVSIIVKK818 pKa = 8.99ATDD821 pKa = 3.52PDD823 pKa = 3.71GAAAADD829 pKa = 3.98TFQLCVDD836 pKa = 4.97GYY838 pKa = 9.14PVSSPAVDD846 pKa = 3.33AAALNVTLYY855 pKa = 10.09PNPTRR860 pKa = 11.84DD861 pKa = 2.99RR862 pKa = 11.84VNIEE866 pKa = 3.9VQNSISGPAEE876 pKa = 3.31ISVFTMDD883 pKa = 4.8GKK885 pKa = 11.01RR886 pKa = 11.84ILQRR890 pKa = 11.84NYY892 pKa = 8.57TDD894 pKa = 2.87NRR896 pKa = 11.84RR897 pKa = 11.84ISFSMKK903 pKa = 8.23EE904 pKa = 4.39HH905 pKa = 5.58VSGMYY910 pKa = 9.26FVKK913 pKa = 10.79LNMQGKK919 pKa = 9.23EE920 pKa = 3.89IVKK923 pKa = 10.6KK924 pKa = 10.71LVLNNKK930 pKa = 8.99

Molecular weight: 98.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A399CY40|A0A399CY40_9BACT Response regulator OS=Mariniphaga sediminis OX=1628158 GN=D1164_19880 PE=4 SV=1
MM1 pKa = 7.3VFYY4 pKa = 10.88SCQYY8 pKa = 10.16TNYY11 pKa = 9.95CFANQPYY18 pKa = 9.58KK19 pKa = 10.93LSIFWGNACMLLYY32 pKa = 10.22ILEE35 pKa = 4.41GNIFFPNLLTRR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84HH49 pKa = 4.93TLFSDD54 pKa = 3.58AKK56 pKa = 10.05VRR58 pKa = 11.84LHH60 pKa = 6.13LRR62 pKa = 11.84PVTSRR67 pKa = 11.84RR68 pKa = 11.84KK69 pKa = 8.36TGWHH73 pKa = 5.91NNWVTGSKK81 pKa = 9.22TEE83 pKa = 4.1NVSRR87 pKa = 11.84RR88 pKa = 11.84QKK90 pKa = 9.92KK91 pKa = 9.15CRR93 pKa = 11.84EE94 pKa = 3.84IILRR98 pKa = 3.99

Molecular weight: 11.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4517

0

4517

1693228

28

3263

374.9

42.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.409 ± 0.035

0.865 ± 0.015

5.275 ± 0.026

7.031 ± 0.035

5.262 ± 0.026

7.109 ± 0.037

1.88 ± 0.016

7.147 ± 0.031

6.883 ± 0.038

9.112 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.352 ± 0.015

5.525 ± 0.027

3.963 ± 0.019

3.337 ± 0.021

4.24 ± 0.025

6.336 ± 0.029

5.331 ± 0.04

6.486 ± 0.024

1.413 ± 0.015

4.044 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski