Desulfosporosinus sp. I2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Desulfosporosinus; unclassified Desulfosporosinus

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5500 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F2JAW2|A0A0F2JAW2_9FIRM Stage V sporulation protein AD (SpoVAD) OS=Desulfosporosinus sp. I2 OX=1617025 GN=UF75_3562 PE=4 SV=1
MM1 pKa = 7.47AAFGYY6 pKa = 10.52VYY8 pKa = 10.32TLDD11 pKa = 4.21AVAQVVAGGADD22 pKa = 3.34VLFSSNGPLVNEE34 pKa = 3.65VHH36 pKa = 6.7IAGTAPVTVALAGNYY51 pKa = 9.72QIDD54 pKa = 4.01YY55 pKa = 9.91SVSITAGVGSEE66 pKa = 3.64IAIAVNGVVDD76 pKa = 4.65PSTPITALVATGQVPGQAILTLAAGDD102 pKa = 4.15VITLRR107 pKa = 11.84NSSAVPFTLTTAPEE121 pKa = 3.91VGAQLTIDD129 pKa = 4.06KK130 pKa = 10.77LDD132 pKa = 3.29

Molecular weight: 13.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F2JDC2|A0A0F2JDC2_9FIRM Site-specific recombinase OS=Desulfosporosinus sp. I2 OX=1617025 GN=UF75_3451 PE=4 SV=1
MM1 pKa = 7.41ILRR4 pKa = 11.84RR5 pKa = 11.84KK6 pKa = 10.12SEE8 pKa = 3.79VLIRR12 pKa = 11.84SVSEE16 pKa = 3.91NPSRR20 pKa = 11.84GPLTIDD26 pKa = 3.33SVIGSVTPRR35 pKa = 11.84LAHH38 pKa = 6.53PLAPITIASVKK49 pKa = 10.06SPKK52 pKa = 10.48KK53 pKa = 7.95MTEE56 pKa = 3.75SPEE59 pKa = 4.15RR60 pKa = 11.84MASTASPILDD70 pKa = 3.08IFFISRR76 pKa = 11.84PGSRR80 pKa = 11.84LWIVISTTFDD90 pKa = 3.15NFTCASSGCPGKK102 pKa = 9.48STIRR106 pKa = 11.84GLRR109 pKa = 11.84INWSSRR115 pKa = 11.84FLSTSPSTATTIQGIRR131 pKa = 11.84SFPWRR136 pKa = 11.84EE137 pKa = 3.36NSRR140 pKa = 11.84NTTSLLLDD148 pKa = 4.4KK149 pKa = 10.86ISFTFFPDD157 pKa = 2.66KK158 pKa = 11.28GFFKK162 pKa = 10.75SSSNEE167 pKa = 3.59YY168 pKa = 10.34EE169 pKa = 3.65GDD171 pKa = 3.32FRR173 pKa = 11.84IFRR176 pKa = 11.84SPFGRR181 pKa = 11.84MVSSLIIFIQLGIVVSSDD199 pKa = 2.56LAYY202 pKa = 10.18FVRR205 pKa = 11.84QRR207 pKa = 11.84VDD209 pKa = 3.01YY210 pKa = 10.88SPVLGSLLYY219 pKa = 10.22SAKK222 pKa = 10.27QNPLPPHH229 pKa = 5.42FSIRR233 pKa = 11.84IRR235 pKa = 3.53

Molecular weight: 26.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5500

0

5500

1418861

37

2965

258.0

28.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.52 ± 0.039

1.124 ± 0.015

4.844 ± 0.025

6.82 ± 0.04

4.051 ± 0.027

7.462 ± 0.037

1.862 ± 0.018

7.47 ± 0.032

6.148 ± 0.033

10.431 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.738 ± 0.02

4.109 ± 0.022

3.922 ± 0.024

3.827 ± 0.024

4.703 ± 0.032

6.083 ± 0.031

5.364 ± 0.03

7.21 ± 0.027

1.092 ± 0.014

3.222 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski