Amino acid dipepetide frequency for Desulfosporosinus sp. I2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.074AlaAla: 6.074 ± 0.094	0.928AlaCys: 0.928 ± 0.027	3.438AlaAsp: 3.438 ± 0.053	4.993AlaGlu: 4.993 ± 0.072	3.03AlaPhe: 3.03 ± 0.05	5.952AlaGly: 5.952 ± 0.074	1.374AlaHis: 1.374 ± 0.035	5.563AlaIle: 5.563 ± 0.074	4.748AlaLys: 4.748 ± 0.06	8.563AlaLeu: 8.563 ± 0.089	2.166AlaMet: 2.166 ± 0.043	2.746AlaAsn: 2.746 ± 0.053	2.296AlaPro: 2.296 ± 0.047	3.043AlaGln: 3.043 ± 0.054	3.453AlaArg: 3.453 ± 0.043	4.129AlaSer: 4.129 ± 0.06	3.863AlaThr: 3.863 ± 0.058	5.741AlaVal: 5.741 ± 0.068	0.794AlaTrp: 0.794 ± 0.023	2.306AlaTyr: 2.306 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	0.74CysAla: 0.74 ± 0.024	0.214CysCys: 0.214 ± 0.013	0.509CysAsp: 0.509 ± 0.02	0.597CysGlu: 0.597 ± 0.022	0.419CysPhe: 0.419 ± 0.019	1.201CysGly: 1.201 ± 0.033	0.296CysHis: 0.296 ± 0.018	0.725CysIle: 0.725 ± 0.026	0.557CysLys: 0.557 ± 0.022	1.059CysLeu: 1.059 ± 0.027	0.288CysMet: 0.288 ± 0.016	0.414CysAsn: 0.414 ± 0.018	0.675CysPro: 0.675 ± 0.026	0.462CysGln: 0.462 ± 0.016	0.559CysArg: 0.559 ± 0.021	0.765CysSer: 0.765 ± 0.025	0.574CysThr: 0.574 ± 0.02	0.714CysVal: 0.714 ± 0.024	0.102CysTrp: 0.102 ± 0.007	0.375CysTyr: 0.375 ± 0.015	0.001CysXaa: 0.001 ± 0.001
Asp	3.191AspAla: 3.191 ± 0.055	0.553AspCys: 0.553 ± 0.018	2.107AspAsp: 2.107 ± 0.047	3.401AspGlu: 3.401 ± 0.059	2.3AspPhe: 2.3 ± 0.043	3.155AspGly: 3.155 ± 0.054	0.939AspHis: 0.939 ± 0.025	3.877AspIle: 3.877 ± 0.059	2.909AspLys: 2.909 ± 0.043	5.409AspLeu: 5.409 ± 0.054	1.282AspMet: 1.282 ± 0.03	1.763AspAsn: 1.763 ± 0.036	2.118AspPro: 2.118 ± 0.038	1.957AspGln: 1.957 ± 0.039	2.379AspArg: 2.379 ± 0.046	2.74AspSer: 2.74 ± 0.052	2.26AspThr: 2.26 ± 0.047	3.473AspVal: 3.473 ± 0.052	0.624AspTrp: 0.624 ± 0.02	2.003AspTyr: 2.003 ± 0.035	0.0AspXaa: 0.0 ± 0.0
Glu	5.108GluAla: 5.108 ± 0.074	0.562GluCys: 0.562 ± 0.021	3.186GluAsp: 3.186 ± 0.051	5.382GluGlu: 5.382 ± 0.073	2.532GluPhe: 2.532 ± 0.048	4.385GluGly: 4.385 ± 0.067	1.293GluHis: 1.293 ± 0.035	5.521GluIle: 5.521 ± 0.069	4.984GluLys: 4.984 ± 0.072	7.059GluLeu: 7.059 ± 0.08	1.978GluMet: 1.978 ± 0.036	3.04GluAsn: 3.04 ± 0.046	1.91GluPro: 1.91 ± 0.04	2.903GluGln: 2.903 ± 0.05	3.809GluArg: 3.809 ± 0.074	3.38GluSer: 3.38 ± 0.05	3.379GluThr: 3.379 ± 0.047	4.986GluVal: 4.986 ± 0.073	0.744GluTrp: 0.744 ± 0.024	2.057GluTyr: 2.057 ± 0.045	0.0GluXaa: 0.0 ± 0.0
Phe	2.995PheAla: 2.995 ± 0.045	0.549PheCys: 0.549 ± 0.022	2.023PheAsp: 2.023 ± 0.036	2.422PheGlu: 2.422 ± 0.046	1.822PhePhe: 1.822 ± 0.043	3.212PheGly: 3.212 ± 0.054	0.708PheHis: 0.708 ± 0.022	2.896PheIle: 2.896 ± 0.055	2.206PheLys: 2.206 ± 0.041	4.254PheLeu: 4.254 ± 0.069	1.128PheMet: 1.128 ± 0.032	1.689PheAsn: 1.689 ± 0.035	1.677PhePro: 1.677 ± 0.032	1.369PheGln: 1.369 ± 0.03	1.752PheArg: 1.752 ± 0.038	2.935PheSer: 2.935 ± 0.047	2.167PheThr: 2.167 ± 0.042	2.778PheVal: 2.778 ± 0.054	0.526PheTrp: 0.526 ± 0.02	1.399PheTyr: 1.399 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	5.421GlyAla: 5.421 ± 0.073	1.058GlyCys: 1.058 ± 0.034	3.208GlyAsp: 3.208 ± 0.056	4.527GlyGlu: 4.527 ± 0.065	3.314GlyPhe: 3.314 ± 0.052	5.464GlyGly: 5.464 ± 0.087	1.401GlyHis: 1.401 ± 0.036	6.378GlyIle: 6.378 ± 0.07	5.164GlyLys: 5.164 ± 0.063	7.585GlyLeu: 7.585 ± 0.087	2.368GlyMet: 2.368 ± 0.042	2.846GlyAsn: 2.846 ± 0.052	1.968GlyPro: 1.968 ± 0.044	2.77GlyGln: 2.77 ± 0.052	3.242GlyArg: 3.242 ± 0.055	4.242GlySer: 4.242 ± 0.065	4.284GlyThr: 4.284 ± 0.059	5.704GlyVal: 5.704 ± 0.077	0.88GlyTrp: 0.88 ± 0.025	2.8GlyTyr: 2.8 ± 0.046	0.0GlyXaa: 0.0 ± 0.0
His	1.22HisAla: 1.22 ± 0.031	0.3HisCys: 0.3 ± 0.014	0.862HisAsp: 0.862 ± 0.022	1.181HisGlu: 1.181 ± 0.03	0.853HisPhe: 0.853 ± 0.026	1.343HisGly: 1.343 ± 0.029	0.454HisHis: 0.454 ± 0.02	1.176HisIle: 1.176 ± 0.03	0.944HisLys: 0.944 ± 0.023	1.988HisLeu: 1.988 ± 0.043	0.478HisMet: 0.478 ± 0.018	0.772HisAsn: 0.772 ± 0.024	1.091HisPro: 1.091 ± 0.026	0.784HisGln: 0.784 ± 0.025	0.934HisArg: 0.934 ± 0.029	1.166HisSer: 1.166 ± 0.03	0.994HisThr: 0.994 ± 0.026	1.126HisVal: 1.126 ± 0.027	0.252HisTrp: 0.252 ± 0.013	0.706HisTyr: 0.706 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	6.013IleAla: 6.013 ± 0.073	0.81IleCys: 0.81 ± 0.025	3.793IleAsp: 3.793 ± 0.052	4.798IleGlu: 4.798 ± 0.056	2.893IlePhe: 2.893 ± 0.047	5.583IleGly: 5.583 ± 0.068	1.429IleHis: 1.429 ± 0.033	5.426IleIle: 5.426 ± 0.075	4.264IleLys: 4.264 ± 0.059	7.754IleLeu: 7.754 ± 0.088	1.839IleMet: 1.839 ± 0.038	3.203IleAsn: 3.203 ± 0.049	3.586IlePro: 3.586 ± 0.057	2.683IleGln: 2.683 ± 0.046	3.484IleArg: 3.484 ± 0.049	5.018IleSer: 5.018 ± 0.068	4.047IleThr: 4.047 ± 0.061	5.199IleVal: 5.199 ± 0.061	0.663IleTrp: 0.663 ± 0.023	2.212IleTyr: 2.212 ± 0.043	0.0IleXaa: 0.0 ± 0.0
Lys	4.757LysAla: 4.757 ± 0.071	0.521LysCys: 0.521 ± 0.02	3.213LysAsp: 3.213 ± 0.048	4.954LysGlu: 4.954 ± 0.06	1.93LysPhe: 1.93 ± 0.043	4.32LysGly: 4.32 ± 0.064	1.032LysHis: 1.032 ± 0.031	4.683LysIle: 4.683 ± 0.06	4.048LysLys: 4.048 ± 0.061	5.751LysLeu: 5.751 ± 0.063	1.799LysMet: 1.799 ± 0.038	2.732LysAsn: 2.732 ± 0.048	2.248LysPro: 2.248 ± 0.038	2.367LysGln: 2.367 ± 0.043	2.994LysArg: 2.994 ± 0.05	3.39LysSer: 3.39 ± 0.052	3.499LysThr: 3.499 ± 0.047	4.739LysVal: 4.739 ± 0.056	0.578LysTrp: 0.578 ± 0.02	1.927LysTyr: 1.927 ± 0.044	0.0LysXaa: 0.0 ± 0.0
Leu	8.655LeuAla: 8.655 ± 0.086	1.059LeuCys: 1.059 ± 0.03	5.219LeuAsp: 5.219 ± 0.06	7.135LeuGlu: 7.135 ± 0.084	4.084LeuPhe: 4.084 ± 0.069	7.954LeuGly: 7.954 ± 0.082	1.777LeuHis: 1.777 ± 0.039	7.302LeuIle: 7.302 ± 0.09	6.61LeuLys: 6.61 ± 0.075	10.376LeuLeu: 10.376 ± 0.119	2.686LeuMet: 2.686 ± 0.047	4.511LeuAsn: 4.511 ± 0.056	4.402LeuPro: 4.402 ± 0.058	3.647LeuGln: 3.647 ± 0.054	4.961LeuArg: 4.961 ± 0.068	7.14LeuSer: 7.14 ± 0.084	5.887LeuThr: 5.887 ± 0.069	7.032LeuVal: 7.032 ± 0.08	1.035LeuTrp: 1.035 ± 0.024	2.84LeuTyr: 2.84 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	2.484MetAla: 2.484 ± 0.044	0.249MetCys: 0.249 ± 0.013	1.527MetAsp: 1.527 ± 0.029	1.859MetGlu: 1.859 ± 0.042	0.906MetPhe: 0.906 ± 0.028	2.233MetGly: 2.233 ± 0.038	0.457MetHis: 0.457 ± 0.019	1.916MetIle: 1.916 ± 0.04	1.791MetLys: 1.791 ± 0.036	2.57MetLeu: 2.57 ± 0.042	0.813MetMet: 0.813 ± 0.026	1.235MetAsn: 1.235 ± 0.031	1.08MetPro: 1.08 ± 0.027	0.894MetGln: 0.894 ± 0.025	1.273MetArg: 1.273 ± 0.03	1.714MetSer: 1.714 ± 0.033	1.576MetThr: 1.576 ± 0.034	1.975MetVal: 1.975 ± 0.039	0.173MetTrp: 0.173 ± 0.011	0.653MetTyr: 0.653 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	2.732AsnAla: 2.732 ± 0.044	0.506AsnCys: 0.506 ± 0.02	1.855AsnAsp: 1.855 ± 0.042	2.453AsnGlu: 2.453 ± 0.037	1.601AsnPhe: 1.601 ± 0.033	2.871AsnGly: 2.871 ± 0.055	0.865AsnHis: 0.865 ± 0.025	3.028AsnIle: 3.028 ± 0.046	2.403AsnLys: 2.403 ± 0.048	4.361AsnLeu: 4.361 ± 0.06	1.078AsnMet: 1.078 ± 0.028	1.785AsnAsn: 1.785 ± 0.046	2.336AsnPro: 2.336 ± 0.043	1.715AsnGln: 1.715 ± 0.037	1.961AsnArg: 1.961 ± 0.04	2.563AsnSer: 2.563 ± 0.049	2.069AsnThr: 2.069 ± 0.042	2.826AsnVal: 2.826 ± 0.046	0.503AsnTrp: 0.503 ± 0.02	1.577AsnTyr: 1.577 ± 0.033	0.0AsnXaa: 0.0 ± 0.0
Pro	2.522ProAla: 2.522 ± 0.051	0.394ProCys: 0.394 ± 0.018	2.171ProAsp: 2.171 ± 0.041	3.25ProGlu: 3.25 ± 0.045	1.731ProPhe: 1.731 ± 0.037	2.827ProGly: 2.827 ± 0.047	0.768ProHis: 0.768 ± 0.022	2.814ProIle: 2.814 ± 0.042	2.2ProLys: 2.2 ± 0.041	4.011ProLeu: 4.011 ± 0.054	0.98ProMet: 0.98 ± 0.026	1.642ProAsn: 1.642 ± 0.036	1.319ProPro: 1.319 ± 0.031	1.522ProGln: 1.522 ± 0.033	1.534ProArg: 1.534 ± 0.037	2.481ProSer: 2.481 ± 0.041	2.167ProThr: 2.167 ± 0.042	3.067ProVal: 3.067 ± 0.051	0.475ProTrp: 0.475 ± 0.019	1.341ProTyr: 1.341 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	3.226GlnAla: 3.226 ± 0.056	0.377GlnCys: 0.377 ± 0.015	1.83GlnAsp: 1.83 ± 0.036	3.041GlnGlu: 3.041 ± 0.057	1.3GlnPhe: 1.3 ± 0.029	2.913GlnGly: 2.913 ± 0.056	0.646GlnHis: 0.646 ± 0.022	2.826GlnIle: 2.826 ± 0.045	2.53GlnLys: 2.53 ± 0.048	3.609GlnLeu: 3.609 ± 0.058	1.086GlnMet: 1.086 ± 0.029	1.514GlnAsn: 1.514 ± 0.036	1.235GlnPro: 1.235 ± 0.036	1.558GlnGln: 1.558 ± 0.038	1.906GlnArg: 1.906 ± 0.038	2.133GlnSer: 2.133 ± 0.044	2.169GlnThr: 2.169 ± 0.049	2.797GlnVal: 2.797 ± 0.049	0.44GlnTrp: 0.44 ± 0.015	1.13GlnTyr: 1.13 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	3.104ArgAla: 3.104 ± 0.051	0.524ArgCys: 0.524 ± 0.021	2.251ArgAsp: 2.251 ± 0.046	3.743ArgGlu: 3.743 ± 0.059	1.985ArgPhe: 1.985 ± 0.039	2.965ArgGly: 2.965 ± 0.049	0.887ArgHis: 0.887 ± 0.024	3.63ArgIle: 3.63 ± 0.05	3.048ArgLys: 3.048 ± 0.039	5.218ArgLeu: 5.218 ± 0.069	1.383ArgMet: 1.383 ± 0.027	1.88ArgAsn: 1.88 ± 0.038	1.617ArgPro: 1.617 ± 0.036	1.968ArgGln: 1.968 ± 0.037	2.406ArgArg: 2.406 ± 0.044	2.496ArgSer: 2.496 ± 0.046	2.305ArgThr: 2.305 ± 0.041	3.441ArgVal: 3.441 ± 0.047	0.557ArgTrp: 0.557 ± 0.023	1.62ArgTyr: 1.62 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	4.175SerAla: 4.175 ± 0.055	0.682SerCys: 0.682 ± 0.023	2.825SerAsp: 2.825 ± 0.043	3.828SerGlu: 3.828 ± 0.055	2.694SerPhe: 2.694 ± 0.042	4.995SerGly: 4.995 ± 0.063	1.096SerHis: 1.096 ± 0.029	4.523SerIle: 4.523 ± 0.056	3.661SerLys: 3.661 ± 0.051	6.57SerLeu: 6.57 ± 0.076	1.716SerMet: 1.716 ± 0.037	2.415SerAsn: 2.415 ± 0.041	2.467SerPro: 2.467 ± 0.039	2.378SerGln: 2.378 ± 0.044	2.722SerArg: 2.722 ± 0.044	4.063SerSer: 4.063 ± 0.068	3.153SerThr: 3.153 ± 0.05	4.183SerVal: 4.183 ± 0.056	0.714SerTrp: 0.714 ± 0.024	1.968SerTyr: 1.968 ± 0.044	0.0SerXaa: 0.0 ± 0.0
Thr	4.099ThrAla: 4.099 ± 0.068	0.559ThrCys: 0.559 ± 0.022	2.569ThrAsp: 2.569 ± 0.05	3.208ThrGlu: 3.208 ± 0.057	2.17ThrPhe: 2.17 ± 0.039	4.704ThrGly: 4.704 ± 0.062	1.029ThrHis: 1.029 ± 0.025	4.005ThrIle: 4.005 ± 0.054	2.874ThrLys: 2.874 ± 0.049	5.851ThrLeu: 5.851 ± 0.076	1.36ThrMet: 1.36 ± 0.034	2.097ThrAsn: 2.097 ± 0.048	2.524ThrPro: 2.524 ± 0.054	1.88ThrGln: 1.88 ± 0.037	2.126ThrArg: 2.126 ± 0.042	3.194ThrSer: 3.194 ± 0.053	3.024ThrThr: 3.024 ± 0.063	4.115ThrVal: 4.115 ± 0.062	0.593ThrTrp: 0.593 ± 0.023	1.658ThrTyr: 1.658 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	5.626ValAla: 5.626 ± 0.07	0.837ValCys: 0.837 ± 0.026	3.59ValAsp: 3.59 ± 0.054	4.755ValGlu: 4.755 ± 0.061	3.051ValPhe: 3.051 ± 0.05	5.18ValGly: 5.18 ± 0.073	1.235ValHis: 1.235 ± 0.03	5.629ValIle: 5.629 ± 0.067	4.149ValLys: 4.149 ± 0.051	7.557ValLeu: 7.557 ± 0.081	1.966ValMet: 1.966 ± 0.036	2.988ValAsn: 2.988 ± 0.046	2.798ValPro: 2.798 ± 0.049	2.498ValGln: 2.498 ± 0.045	3.271ValArg: 3.271 ± 0.054	4.604ValSer: 4.604 ± 0.061	4.05ValThr: 4.05 ± 0.067	5.468ValVal: 5.468 ± 0.073	0.717ValTrp: 0.717 ± 0.024	2.124ValTyr: 2.124 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	0.78TrpAla: 0.78 ± 0.026	0.1TrpCys: 0.1 ± 0.01	0.569TrpAsp: 0.569 ± 0.022	0.737TrpGlu: 0.737 ± 0.02	0.408TrpPhe: 0.408 ± 0.019	0.84TrpGly: 0.84 ± 0.026	0.203TrpHis: 0.203 ± 0.011	0.766TrpIle: 0.766 ± 0.022	0.644TrpLys: 0.644 ± 0.024	1.205TrpLeu: 1.205 ± 0.033	0.285TrpMet: 0.285 ± 0.015	0.529TrpAsn: 0.529 ± 0.019	0.379TrpPro: 0.379 ± 0.016	0.546TrpGln: 0.546 ± 0.022	0.539TrpArg: 0.539 ± 0.024	0.616TrpSer: 0.616 ± 0.023	0.509TrpThr: 0.509 ± 0.021	0.783TrpVal: 0.783 ± 0.028	0.161TrpTrp: 0.161 ± 0.01	0.316TrpTyr: 0.316 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.28TyrAla: 2.28 ± 0.041	0.462TyrCys: 0.462 ± 0.019	1.697TyrAsp: 1.697 ± 0.047	1.935TyrGlu: 1.935 ± 0.043	1.483TyrPhe: 1.483 ± 0.036	2.527TyrGly: 2.527 ± 0.044	0.737TyrHis: 0.737 ± 0.021	2.011TyrIle: 2.011 ± 0.036	1.745TyrLys: 1.745 ± 0.035	3.563TyrLeu: 3.563 ± 0.059	0.698TyrMet: 0.698 ± 0.021	1.286TyrAsn: 1.286 ± 0.033	1.488TyrPro: 1.488 ± 0.032	1.321TyrGln: 1.321 ± 0.031	1.72TyrArg: 1.72 ± 0.039	2.059TyrSer: 2.059 ± 0.042	1.662TyrThr: 1.662 ± 0.034	1.952TyrVal: 1.952 ± 0.042	0.386TyrTrp: 0.386 ± 0.018	1.209TyrTyr: 1.209 ± 0.033	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5500 proteins (1418861 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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