Lactobacillus similis DSM 23365 = JCM 2765

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Secundilactobacillus; Secundilactobacillus similis

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3000 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R2EZF4|A0A0R2EZF4_9LACO Purine nucleosidase OS=Lactobacillus similis DSM 23365 = JCM 2765 OX=1423804 GN=FD14_GL000927 PE=4 SV=1
MM1 pKa = 7.18VNVDD5 pKa = 4.07GLYY8 pKa = 10.6SDD10 pKa = 3.94WDD12 pKa = 4.34SIAKK16 pKa = 9.31TSIGSSNLAITGTGSHH32 pKa = 6.09VYY34 pKa = 9.89FYY36 pKa = 11.7VDD38 pKa = 3.31ASKK41 pKa = 10.85QSSAVKK47 pKa = 10.46LSDD50 pKa = 3.55YY51 pKa = 10.28NVKK54 pKa = 10.21IGSSTYY60 pKa = 10.22KK61 pKa = 10.16ISLSGSGEE69 pKa = 3.97NYY71 pKa = 9.67ILQGQDD77 pKa = 2.73QTTGNSTTLNDD88 pKa = 2.89IGAAVVKK95 pKa = 10.27DD96 pKa = 3.81NKK98 pKa = 10.34IEE100 pKa = 4.11ADD102 pKa = 3.16IDD104 pKa = 3.97LTKK107 pKa = 10.89LPEE110 pKa = 4.45TFNGMQTLTILNDD123 pKa = 3.59SLGSGEE129 pKa = 4.17ATSLNDD135 pKa = 3.36ISTTAATDD143 pKa = 3.72LANEE147 pKa = 4.24VAMPTLASGSSDD159 pKa = 3.56TIADD163 pKa = 3.75SSNVASATNSMTTSGITIDD182 pKa = 5.28GDD184 pKa = 3.64DD185 pKa = 4.15SDD187 pKa = 4.19WDD189 pKa = 3.73NVAKK193 pKa = 10.44IQTGYY198 pKa = 10.94SGTNDD203 pKa = 2.97VAVATDD209 pKa = 3.44SKK211 pKa = 11.11HH212 pKa = 6.15VYY214 pKa = 10.08FYY216 pKa = 11.91VNGTNSTSPVATNSYY231 pKa = 9.71QLNIGSNGYY240 pKa = 9.06YY241 pKa = 10.29VSLISNGDD249 pKa = 2.96GTYY252 pKa = 10.78NVQAQDD258 pKa = 3.45QSNNWVQLGTIGTATIKK275 pKa = 11.14NNVLEE280 pKa = 4.18AQINLSDD287 pKa = 4.67LPQKK291 pKa = 10.82YY292 pKa = 10.27SGVQTVSLSNQQVGPGTATNMVII315 pKa = 4.62

Molecular weight: 32.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R2FH44|A0A0R2FH44_9LACO Isochorismatase domain-containing protein OS=Lactobacillus similis DSM 23365 = JCM 2765 OX=1423804 GN=FD14_GL000590 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.69QPKK8 pKa = 8.59KK9 pKa = 7.38RR10 pKa = 11.84HH11 pKa = 5.81RR12 pKa = 11.84SRR14 pKa = 11.84VHH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 10.06RR21 pKa = 11.84MATSNGRR28 pKa = 11.84KK29 pKa = 8.51VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.39GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3000

0

3000

923721

44

3420

307.9

34.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.022 ± 0.051

0.461 ± 0.013

5.926 ± 0.047

4.946 ± 0.054

3.971 ± 0.034

6.866 ± 0.044

2.218 ± 0.026

6.351 ± 0.047

5.243 ± 0.048

9.841 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.564 ± 0.024

4.349 ± 0.037

3.856 ± 0.038

5.028 ± 0.044

4.194 ± 0.039

5.718 ± 0.042

7.437 ± 0.099

7.469 ± 0.041

1.092 ± 0.019

3.433 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski