Amino acid dipepetide frequency for Lactobacillus similis DSM 23365 = JCM 2765

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.501AlaAla: 8.501 ± 0.117	0.392AlaCys: 0.392 ± 0.022	6.031AlaAsp: 6.031 ± 0.088	4.72AlaGlu: 4.72 ± 0.087	3.401AlaPhe: 3.401 ± 0.071	6.613AlaGly: 6.613 ± 0.102	1.835AlaHis: 1.835 ± 0.051	6.156AlaIle: 6.156 ± 0.099	5.377AlaLys: 5.377 ± 0.094	8.597AlaLeu: 8.597 ± 0.113	2.449AlaMet: 2.449 ± 0.058	3.937AlaAsn: 3.937 ± 0.074	2.849AlaPro: 2.849 ± 0.069	4.035AlaGln: 4.035 ± 0.067	2.957AlaArg: 2.957 ± 0.061	4.873AlaSer: 4.873 ± 0.089	7.261AlaThr: 7.261 ± 0.152	6.621AlaVal: 6.621 ± 0.097	0.766AlaTrp: 0.766 ± 0.034	2.843AlaTyr: 2.843 ± 0.065	0.001AlaXaa: 0.001 ± 0.001
Cys	0.333CysAla: 0.333 ± 0.021	0.048CysCys: 0.048 ± 0.008	0.266CysAsp: 0.266 ± 0.018	0.237CysGlu: 0.237 ± 0.016	0.217CysPhe: 0.217 ± 0.015	0.462CysGly: 0.462 ± 0.025	0.155CysHis: 0.155 ± 0.015	0.252CysIle: 0.252 ± 0.018	0.12CysLys: 0.12 ± 0.012	0.514CysLeu: 0.514 ± 0.026	0.108CysMet: 0.108 ± 0.009	0.15CysAsn: 0.15 ± 0.012	0.223CysPro: 0.223 ± 0.017	0.252CysGln: 0.252 ± 0.018	0.207CysArg: 0.207 ± 0.016	0.242CysSer: 0.242 ± 0.015	0.252CysThr: 0.252 ± 0.015	0.325CysVal: 0.325 ± 0.021	0.074CysTrp: 0.074 ± 0.009	0.17CysTyr: 0.17 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	5.309AspAla: 5.309 ± 0.086	0.311AspCys: 0.311 ± 0.02	4.0AspAsp: 4.0 ± 0.09	3.757AspGlu: 3.757 ± 0.076	2.492AspPhe: 2.492 ± 0.053	4.081AspGly: 4.081 ± 0.106	1.586AspHis: 1.586 ± 0.042	3.212AspIle: 3.212 ± 0.066	2.784AspLys: 2.784 ± 0.061	5.508AspLeu: 5.508 ± 0.1	1.448AspMet: 1.448 ± 0.042	2.382AspAsn: 2.382 ± 0.06	2.48AspPro: 2.48 ± 0.06	3.545AspGln: 3.545 ± 0.075	2.598AspArg: 2.598 ± 0.053	2.885AspSer: 2.885 ± 0.078	3.345AspThr: 3.345 ± 0.077	4.252AspVal: 4.252 ± 0.071	0.82AspTrp: 0.82 ± 0.03	2.46AspTyr: 2.46 ± 0.063	0.0AspXaa: 0.0 ± 0.0
Glu	4.795GluAla: 4.795 ± 0.088	0.165GluCys: 0.165 ± 0.015	2.456GluAsp: 2.456 ± 0.06	2.368GluGlu: 2.368 ± 0.063	1.801GluPhe: 1.801 ± 0.056	2.502GluGly: 2.502 ± 0.052	1.11GluHis: 1.11 ± 0.04	3.266GluIle: 3.266 ± 0.075	2.807GluLys: 2.807 ± 0.065	5.532GluLeu: 5.532 ± 0.103	1.531GluMet: 1.531 ± 0.043	2.216GluAsn: 2.216 ± 0.049	1.903GluPro: 1.903 ± 0.06	2.726GluGln: 2.726 ± 0.055	2.646GluArg: 2.646 ± 0.059	2.505GluSer: 2.505 ± 0.057	3.894GluThr: 3.894 ± 0.08	3.416GluVal: 3.416 ± 0.066	0.503GluTrp: 0.503 ± 0.023	1.323GluTyr: 1.323 ± 0.041	0.0GluXaa: 0.0 ± 0.0
Phe	3.165PheAla: 3.165 ± 0.06	0.262PheCys: 0.262 ± 0.018	2.966PheAsp: 2.966 ± 0.068	2.194PheGlu: 2.194 ± 0.05	1.66PhePhe: 1.66 ± 0.047	3.262PheGly: 3.262 ± 0.065	0.734PheHis: 0.734 ± 0.028	2.404PheIle: 2.404 ± 0.061	2.277PheLys: 2.277 ± 0.055	3.438PheLeu: 3.438 ± 0.075	0.993PheMet: 0.993 ± 0.034	2.04PheAsn: 2.04 ± 0.048	1.322PhePro: 1.322 ± 0.042	1.482PheGln: 1.482 ± 0.041	1.328PheArg: 1.328 ± 0.036	2.688PheSer: 2.688 ± 0.056	2.516PheThr: 2.516 ± 0.063	2.879PheVal: 2.879 ± 0.063	0.586PheTrp: 0.586 ± 0.025	1.512PheTyr: 1.512 ± 0.041	0.001PheXaa: 0.001 ± 0.001
Gly	5.305GlyAla: 5.305 ± 0.087	0.39GlyCys: 0.39 ± 0.022	3.844GlyAsp: 3.844 ± 0.07	3.394GlyGlu: 3.394 ± 0.069	3.04GlyPhe: 3.04 ± 0.056	4.537GlyGly: 4.537 ± 0.106	1.635GlyHis: 1.635 ± 0.043	5.378GlyIle: 5.378 ± 0.085	3.883GlyLys: 3.883 ± 0.08	6.879GlyLeu: 6.879 ± 0.094	1.877GlyMet: 1.877 ± 0.051	2.931GlyAsn: 2.931 ± 0.07	1.675GlyPro: 1.675 ± 0.05	3.196GlyGln: 3.196 ± 0.066	2.722GlyArg: 2.722 ± 0.068	3.901GlySer: 3.901 ± 0.079	4.965GlyThr: 4.965 ± 0.123	5.358GlyVal: 5.358 ± 0.073	0.855GlyTrp: 0.855 ± 0.028	2.899GlyTyr: 2.899 ± 0.065	0.002GlyXaa: 0.002 ± 0.002
His	1.681HisAla: 1.681 ± 0.048	0.118HisCys: 0.118 ± 0.011	1.393HisAsp: 1.393 ± 0.043	1.144HisGlu: 1.144 ± 0.038	1.109HisPhe: 1.109 ± 0.037	1.532HisGly: 1.532 ± 0.047	0.82HisHis: 0.82 ± 0.038	1.191HisIle: 1.191 ± 0.036	0.766HisLys: 0.766 ± 0.031	2.279HisLeu: 2.279 ± 0.053	0.509HisMet: 0.509 ± 0.022	0.833HisAsn: 0.833 ± 0.03	1.103HisPro: 1.103 ± 0.041	1.398HisGln: 1.398 ± 0.036	1.154HisArg: 1.154 ± 0.045	0.999HisSer: 0.999 ± 0.034	1.128HisThr: 1.128 ± 0.04	1.706HisVal: 1.706 ± 0.044	0.29HisTrp: 0.29 ± 0.018	1.025HisTyr: 1.025 ± 0.038	0.0HisXaa: 0.0 ± 0.0
Ile	6.045IleAla: 6.045 ± 0.098	0.39IleCys: 0.39 ± 0.023	4.144IleAsp: 4.144 ± 0.078	3.305IleGlu: 3.305 ± 0.07	2.405IlePhe: 2.405 ± 0.065	5.138IleGly: 5.138 ± 0.099	1.258IleHis: 1.258 ± 0.036	3.946IleIle: 3.946 ± 0.083	3.366IleLys: 3.366 ± 0.07	5.556IleLeu: 5.556 ± 0.09	1.481IleMet: 1.481 ± 0.04	3.067IleAsn: 3.067 ± 0.054	2.35IlePro: 2.35 ± 0.054	2.618IleGln: 2.618 ± 0.055	2.493IleArg: 2.493 ± 0.054	4.05IleSer: 4.05 ± 0.063	4.504IleThr: 4.504 ± 0.085	4.882IleVal: 4.882 ± 0.088	0.597IleTrp: 0.597 ± 0.027	1.91IleTyr: 1.91 ± 0.049	0.001IleXaa: 0.001 ± 0.001
Lys	4.877LysAla: 4.877 ± 0.087	0.113LysCys: 0.113 ± 0.01	2.622LysAsp: 2.622 ± 0.071	2.358LysGlu: 2.358 ± 0.06	1.583LysPhe: 1.583 ± 0.045	2.828LysGly: 2.828 ± 0.064	1.171LysHis: 1.171 ± 0.038	2.975LysIle: 2.975 ± 0.064	3.111LysLys: 3.111 ± 0.079	5.228LysLeu: 5.228 ± 0.088	1.637LysMet: 1.637 ± 0.039	2.35LysAsn: 2.35 ± 0.054	2.114LysPro: 2.114 ± 0.055	3.435LysGln: 3.435 ± 0.074	3.031LysArg: 3.031 ± 0.06	2.87LysSer: 2.87 ± 0.068	4.089LysThr: 4.089 ± 0.087	3.668LysVal: 3.668 ± 0.08	0.631LysTrp: 0.631 ± 0.026	1.741LysTyr: 1.741 ± 0.051	0.001LysXaa: 0.001 ± 0.001
Leu	9.855LeuAla: 9.855 ± 0.131	0.459LeuCys: 0.459 ± 0.026	5.293LeuAsp: 5.293 ± 0.099	3.923LeuGlu: 3.923 ± 0.074	3.868LeuPhe: 3.868 ± 0.084	6.832LeuGly: 6.832 ± 0.09	1.721LeuHis: 1.721 ± 0.042	6.667LeuIle: 6.667 ± 0.105	5.703LeuLys: 5.703 ± 0.084	9.329LeuLeu: 9.329 ± 0.136	2.687LeuMet: 2.687 ± 0.059	4.557LeuAsn: 4.557 ± 0.071	4.442LeuPro: 4.442 ± 0.08	3.885LeuGln: 3.885 ± 0.08	3.81LeuArg: 3.81 ± 0.08	6.451LeuSer: 6.451 ± 0.103	8.267LeuThr: 8.267 ± 0.123	7.102LeuVal: 7.102 ± 0.098	0.892LeuTrp: 0.892 ± 0.035	2.669LeuTyr: 2.669 ± 0.06	0.002LeuXaa: 0.002 ± 0.001
Met	2.582MetAla: 2.582 ± 0.061	0.119MetCys: 0.119 ± 0.011	1.235MetAsp: 1.235 ± 0.042	0.868MetGlu: 0.868 ± 0.031	0.828MetPhe: 0.828 ± 0.037	1.837MetGly: 1.837 ± 0.046	0.432MetHis: 0.432 ± 0.021	1.858MetIle: 1.858 ± 0.044	1.542MetLys: 1.542 ± 0.041	2.29MetLeu: 2.29 ± 0.056	0.828MetMet: 0.828 ± 0.029	1.172MetAsn: 1.172 ± 0.034	1.137MetPro: 1.137 ± 0.035	1.126MetGln: 1.126 ± 0.036	0.987MetArg: 0.987 ± 0.03	1.643MetSer: 1.643 ± 0.044	2.411MetThr: 2.411 ± 0.042	1.92MetVal: 1.92 ± 0.051	0.212MetTrp: 0.212 ± 0.014	0.612MetTyr: 0.612 ± 0.026	0.001MetXaa: 0.001 ± 0.001
Asn	3.631AsnAla: 3.631 ± 0.078	0.245AsnCys: 0.245 ± 0.019	2.662AsnAsp: 2.662 ± 0.052	2.277AsnGlu: 2.277 ± 0.056	1.683AsnPhe: 1.683 ± 0.043	3.571AsnGly: 3.571 ± 0.072	1.191AsnHis: 1.191 ± 0.039	2.208AsnIle: 2.208 ± 0.052	1.863AsnLys: 1.863 ± 0.056	3.997AsnLeu: 3.997 ± 0.072	1.041AsnMet: 1.041 ± 0.034	1.866AsnAsn: 1.866 ± 0.049	2.076AsnPro: 2.076 ± 0.049	2.725AsnGln: 2.725 ± 0.065	2.086AsnArg: 2.086 ± 0.054	2.153AsnSer: 2.153 ± 0.065	2.548AsnThr: 2.548 ± 0.061	3.321AsnVal: 3.321 ± 0.061	0.644AsnTrp: 0.644 ± 0.029	1.703AsnTyr: 1.703 ± 0.052	0.001AsnXaa: 0.001 ± 0.001
Pro	3.773ProAla: 3.773 ± 0.079	0.116ProCys: 0.116 ± 0.011	2.699ProAsp: 2.699 ± 0.071	2.886ProGlu: 2.886 ± 0.061	1.608ProPhe: 1.608 ± 0.042	2.416ProGly: 2.416 ± 0.057	0.732ProHis: 0.732 ± 0.029	2.46ProIle: 2.46 ± 0.051	2.22ProLys: 2.22 ± 0.059	3.43ProLeu: 3.43 ± 0.07	0.821ProMet: 0.821 ± 0.034	1.787ProAsn: 1.787 ± 0.045	0.611ProPro: 0.611 ± 0.029	1.721ProGln: 1.721 ± 0.047	1.12ProArg: 1.12 ± 0.038	2.071ProSer: 2.071 ± 0.056	3.098ProThr: 3.098 ± 0.086	3.398ProVal: 3.398 ± 0.074	0.371ProTrp: 0.371 ± 0.02	1.225ProTyr: 1.225 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	4.755GlnAla: 4.755 ± 0.071	0.146GlnCys: 0.146 ± 0.013	2.026GlnAsp: 2.026 ± 0.045	1.992GlnGlu: 1.992 ± 0.05	2.081GlnPhe: 2.081 ± 0.059	2.336GlnGly: 2.336 ± 0.052	1.277GlnHis: 1.277 ± 0.036	2.987GlnIle: 2.987 ± 0.071	2.266GlnLys: 2.266 ± 0.053	6.624GlnLeu: 6.624 ± 0.14	1.232GlnMet: 1.232 ± 0.037	1.876GlnAsn: 1.876 ± 0.047	2.211GlnPro: 2.211 ± 0.056	3.421GlnGln: 3.421 ± 0.097	2.805GlnArg: 2.805 ± 0.073	2.814GlnSer: 2.814 ± 0.067	3.706GlnThr: 3.706 ± 0.081	3.66GlnVal: 3.66 ± 0.07	0.461GlnTrp: 0.461 ± 0.022	1.608GlnTyr: 1.608 ± 0.042	0.0GlnXaa: 0.0 ± 0.0
Arg	3.014ArgAla: 3.014 ± 0.055	0.189ArgCys: 0.189 ± 0.019	2.362ArgAsp: 2.362 ± 0.059	2.448ArgGlu: 2.448 ± 0.064	1.937ArgPhe: 1.937 ± 0.049	2.329ArgGly: 2.329 ± 0.048	1.185ArgHis: 1.185 ± 0.04	2.568ArgIle: 2.568 ± 0.055	2.107ArgLys: 2.107 ± 0.054	4.697ArgLeu: 4.697 ± 0.089	1.119ArgMet: 1.119 ± 0.038	1.743ArgAsn: 1.743 ± 0.051	1.545ArgPro: 1.545 ± 0.044	2.776ArgGln: 2.776 ± 0.071	2.208ArgArg: 2.208 ± 0.058	1.985ArgSer: 1.985 ± 0.051	2.373ArgThr: 2.373 ± 0.047	2.999ArgVal: 2.999 ± 0.069	0.483ArgTrp: 0.483 ± 0.025	1.87ArgTyr: 1.87 ± 0.052	0.001ArgXaa: 0.001 ± 0.001
Ser	4.907SerAla: 4.907 ± 0.082	0.237SerCys: 0.237 ± 0.017	3.549SerAsp: 3.549 ± 0.068	2.929SerGlu: 2.929 ± 0.077	2.486SerPhe: 2.486 ± 0.053	4.624SerGly: 4.624 ± 0.082	1.238SerHis: 1.238 ± 0.038	3.489SerIle: 3.489 ± 0.07	2.894SerLys: 2.894 ± 0.067	5.514SerLeu: 5.514 ± 0.093	1.419SerMet: 1.419 ± 0.047	2.352SerAsn: 2.352 ± 0.054	1.869SerPro: 1.869 ± 0.051	2.829SerGln: 2.829 ± 0.052	2.351SerArg: 2.351 ± 0.057	3.49SerSer: 3.49 ± 0.097	4.035SerThr: 4.035 ± 0.114	4.273SerVal: 4.273 ± 0.076	0.671SerTrp: 0.671 ± 0.027	2.019SerTyr: 2.019 ± 0.054	0.0SerXaa: 0.0 ± 0.0
Thr	7.039ThrAla: 7.039 ± 0.174	0.245ThrCys: 0.245 ± 0.018	4.718ThrAsp: 4.718 ± 0.107	3.387ThrGlu: 3.387 ± 0.085	2.581ThrPhe: 2.581 ± 0.051	5.468ThrGly: 5.468 ± 0.136	1.479ThrHis: 1.479 ± 0.046	4.941ThrIle: 4.941 ± 0.108	3.686ThrLys: 3.686 ± 0.077	6.775ThrLeu: 6.775 ± 0.093	1.585ThrMet: 1.585 ± 0.04	3.06ThrAsn: 3.06 ± 0.075	3.673ThrPro: 3.673 ± 0.104	3.176ThrGln: 3.176 ± 0.073	2.328ThrArg: 2.328 ± 0.051	4.287ThrSer: 4.287 ± 0.107	6.622ThrThr: 6.622 ± 0.248	6.059ThrVal: 6.059 ± 0.18	0.687ThrTrp: 0.687 ± 0.026	2.569ThrTyr: 2.569 ± 0.08	0.0ThrXaa: 0.0 ± 0.0
Val	7.351ValAla: 7.351 ± 0.101	0.42ValCys: 0.42 ± 0.021	4.441ValAsp: 4.441 ± 0.07	3.28ValGlu: 3.28 ± 0.065	2.725ValPhe: 2.725 ± 0.067	5.186ValGly: 5.186 ± 0.093	1.292ValHis: 1.292 ± 0.035	5.242ValIle: 5.242 ± 0.09	3.985ValLys: 3.985 ± 0.065	6.854ValLeu: 6.854 ± 0.107	1.942ValMet: 1.942 ± 0.053	3.339ValAsn: 3.339 ± 0.057	3.214ValPro: 3.214 ± 0.065	2.778ValGln: 2.778 ± 0.054	2.573ValArg: 2.573 ± 0.06	4.794ValSer: 4.794 ± 0.087	6.482ValThr: 6.482 ± 0.173	5.766ValVal: 5.766 ± 0.092	0.709ValTrp: 0.709 ± 0.028	2.318ValTyr: 2.318 ± 0.055	0.001ValXaa: 0.001 ± 0.001
Trp	0.725TrpAla: 0.725 ± 0.029	0.069TrpCys: 0.069 ± 0.009	0.464TrpAsp: 0.464 ± 0.023	0.375TrpGlu: 0.375 ± 0.018	0.53TrpPhe: 0.53 ± 0.022	0.68TrpGly: 0.68 ± 0.029	0.366TrpHis: 0.366 ± 0.022	0.645TrpIle: 0.645 ± 0.026	0.317TrpLys: 0.317 ± 0.019	1.626TrpLeu: 1.626 ± 0.057	0.249TrpMet: 0.249 ± 0.018	0.425TrpAsn: 0.425 ± 0.022	0.358TrpPro: 0.358 ± 0.022	0.845TrpGln: 0.845 ± 0.036	0.607TrpArg: 0.607 ± 0.029	0.668TrpSer: 0.668 ± 0.022	0.618TrpThr: 0.618 ± 0.026	0.758TrpVal: 0.758 ± 0.031	0.201TrpTrp: 0.201 ± 0.016	0.393TrpTyr: 0.393 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.57TyrAla: 2.57 ± 0.054	0.175TyrCys: 0.175 ± 0.014	2.083TyrAsp: 2.083 ± 0.056	1.622TyrGlu: 1.622 ± 0.05	1.675TyrPhe: 1.675 ± 0.043	2.429TyrGly: 2.429 ± 0.056	0.961TyrHis: 0.961 ± 0.031	1.662TyrIle: 1.662 ± 0.046	1.358TyrLys: 1.358 ± 0.043	3.746TyrLeu: 3.746 ± 0.069	0.682TyrMet: 0.682 ± 0.024	1.41TyrAsn: 1.41 ± 0.043	1.408TyrPro: 1.408 ± 0.049	2.312TyrGln: 2.312 ± 0.054	1.924TyrArg: 1.924 ± 0.05	1.805TyrSer: 1.805 ± 0.048	2.251TyrThr: 2.251 ± 0.089	2.328TyrVal: 2.328 ± 0.055	0.468TyrTrp: 0.468 ± 0.025	1.463TyrTyr: 1.463 ± 0.047	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.001XaaAsn: 0.001 ± 0.001	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.002XaaArg: 0.002 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.14XaaXaa: 0.14 ± 0.054

Statistics based on 3000 proteins (923721 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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