Peptoniphilus coxii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellales; Peptoniphilaceae; Peptoniphilus

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1860 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A134AKK1|A0A134AKK1_9FIRM Cyclic nucleotide-binding domain protein OS=Peptoniphilus coxii OX=755172 GN=HMPREF1863_00253 PE=4 SV=1
MM1 pKa = 7.54LNLKK5 pKa = 10.11EE6 pKa = 4.28GEE8 pKa = 4.19KK9 pKa = 10.38EE10 pKa = 4.14VAVEE14 pKa = 3.86LLEE17 pKa = 4.24YY18 pKa = 9.79EE19 pKa = 4.5YY20 pKa = 10.67PPGWGAGMPYY30 pKa = 9.3DD31 pKa = 4.05TDD33 pKa = 4.32YY34 pKa = 11.02IICKK38 pKa = 9.09IHH40 pKa = 6.83GRR42 pKa = 11.84DD43 pKa = 3.45GDD45 pKa = 4.23DD46 pKa = 3.34EE47 pKa = 5.44FEE49 pKa = 5.75LEE51 pKa = 3.83DD52 pKa = 3.54SYY54 pKa = 11.94FRR56 pKa = 11.84FSEE59 pKa = 3.93IEE61 pKa = 4.29SIYY64 pKa = 9.6EE65 pKa = 3.85NCVEE69 pKa = 4.05ILDD72 pKa = 4.2GEE74 pKa = 4.82SYY76 pKa = 10.93GFEE79 pKa = 4.5AIIEE83 pKa = 4.2NPDD86 pKa = 2.94LGFYY90 pKa = 10.71VEE92 pKa = 5.72IEE94 pKa = 4.39DD95 pKa = 4.64GDD97 pKa = 4.35LAVHH101 pKa = 6.17FQLAGEE107 pKa = 4.64DD108 pKa = 3.69LFSVCEE114 pKa = 3.9LMNPVEE120 pKa = 4.22FRR122 pKa = 11.84EE123 pKa = 4.27MVDD126 pKa = 5.46SIDD129 pKa = 3.39EE130 pKa = 4.2DD131 pKa = 3.66LRR133 pKa = 11.84SIARR137 pKa = 11.84MM138 pKa = 3.49

Molecular weight: 15.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A134AKX9|A0A134AKX9_9FIRM Uncharacterized protein OS=Peptoniphilus coxii OX=755172 GN=HMPREF1863_00083 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 9.46QPKK8 pKa = 8.47RR9 pKa = 11.84KK10 pKa = 8.48QRR12 pKa = 11.84KK13 pKa = 7.82RR14 pKa = 11.84EE15 pKa = 3.67HH16 pKa = 6.2GFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84GGRR28 pKa = 11.84KK29 pKa = 7.54VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.08GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1860

0

1860

564815

37

2850

303.7

34.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.309 ± 0.059

0.846 ± 0.019

6.311 ± 0.056

7.68 ± 0.077

4.322 ± 0.046

7.238 ± 0.05

1.886 ± 0.026

7.462 ± 0.056

7.616 ± 0.084

9.269 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.872 ± 0.035

4.279 ± 0.049

3.45 ± 0.035

2.637 ± 0.035

4.721 ± 0.057

5.736 ± 0.047

5.105 ± 0.051

6.849 ± 0.05

0.7 ± 0.019

3.712 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski