Lutispora thermophila DSM 19022
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3409 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M6H2B0|A0A1M6H2B0_9CLOT Uncharacterized protein OS=Lutispora thermophila DSM 19022 OX=1122184 GN=SAMN02745176_02615 PE=4 SV=1
MM1 pKa = 7.3 QIIEE5 pKa = 4.15 GLYY8 pKa = 9.21 YY9 pKa = 10.87 SKK11 pKa = 10.93 DD12 pKa = 3.67 HH13 pKa = 6.38 EE14 pKa = 4.54 WVKK17 pKa = 11.07 VEE19 pKa = 3.84 GDD21 pKa = 3.19 KK22 pKa = 11.17 AYY24 pKa = 11.24 VGISDD29 pKa = 4.61 FAQDD33 pKa = 3.56 ALGDD37 pKa = 3.75 IVFVEE42 pKa = 4.55 LPEE45 pKa = 5.67 IGTNLIQGEE54 pKa = 4.28 ALGSIEE60 pKa = 4.3 SVKK63 pKa = 10.58 AASDD67 pKa = 3.93 VYY69 pKa = 10.92 CPVSGKK75 pKa = 9.78 VIKK78 pKa = 10.87 VNEE81 pKa = 4.15 ILVDD85 pKa = 4.69 DD86 pKa = 4.58 PALLNEE92 pKa = 4.68 DD93 pKa = 4.6 PYY95 pKa = 10.05 EE96 pKa = 3.8 NWMICVEE103 pKa = 4.2 LTEE106 pKa = 5.46 KK107 pKa = 10.96 DD108 pKa = 3.87 QLDD111 pKa = 3.57 QLMNSAEE118 pKa = 4.19 YY119 pKa = 10.81 EE120 pKa = 4.14 DD121 pKa = 4.41 FCNEE125 pKa = 3.74 EE126 pKa = 3.95 AA127 pKa = 4.93
Molecular weight: 14.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.808
IPC_protein 3.757
Toseland 3.567
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.63
Rodwell 3.592
Grimsley 3.478
Solomon 3.719
Lehninger 3.668
Nozaki 3.846
DTASelect 4.012
Thurlkill 3.605
EMBOSS 3.643
Sillero 3.872
Patrickios 0.947
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.783
Protein with the highest isoelectric point:
>tr|A0A1M6EVL7|A0A1M6EVL7_9CLOT L-fuculose-phosphate aldolase OS=Lutispora thermophila DSM 19022 OX=1122184 GN=SAMN02745176_01724 PE=4 SV=1
MM1 pKa = 7.79 LILTVTCFFRR11 pKa = 11.84 IQRR14 pKa = 11.84 PPTWKK19 pKa = 8.49 TRR21 pKa = 11.84 PFEE24 pKa = 3.91 VLARR28 pKa = 11.84 MVILRR33 pKa = 11.84 FLAPNWCVDD42 pKa = 3.22 AYY44 pKa = 10.23 ILFVPTANPEE54 pKa = 3.96 ASDD57 pKa = 3.62 FDD59 pKa = 4.42 LMEE62 pKa = 4.49 FLSPAVSTSVTPPSCTALMDD82 pKa = 4.18 CFHH85 pKa = 7.38 FFPSGPFSVRR95 pKa = 11.84 NRR97 pKa = 11.84 STVDD101 pKa = 2.76 SRR103 pKa = 11.84 TASAIAALMPFLSMSASCRR122 pKa = 11.84 SLFISLLVSPSSLMLYY138 pKa = 9.81 IYY140 pKa = 10.37 IPFRR144 pKa = 11.84 LSRR147 pKa = 11.84 EE148 pKa = 3.7 KK149 pKa = 10.73 RR150 pKa = 11.84 RR151 pKa = 11.84 GLDD154 pKa = 3.15 ATEE157 pKa = 4.28 KK158 pKa = 9.33 PVRR161 pKa = 11.84 AAVVVSTAPISTFPSASNSKK181 pKa = 10.01 NRR183 pKa = 11.84 IAALYY188 pKa = 9.81 RR189 pKa = 11.84 FRR191 pKa = 6.04
Molecular weight: 21.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 9.341
IPC_protein 9.926
Toseland 9.999
ProMoST 10.043
Dawson 10.248
Bjellqvist 10.043
Wikipedia 10.467
Rodwell 10.365
Grimsley 10.335
Solomon 10.321
Lehninger 10.277
Nozaki 10.175
DTASelect 9.999
Thurlkill 10.101
EMBOSS 10.438
Sillero 10.204
Patrickios 9.882
IPC_peptide 10.321
IPC2_peptide 9.443
IPC2.peptide.svr19 8.326
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3409
0
3409
1016382
39
2664
298.1
33.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.373 ± 0.05
1.14 ± 0.018
5.736 ± 0.031
7.465 ± 0.042
4.113 ± 0.029
6.567 ± 0.042
1.491 ± 0.017
9.654 ± 0.049
8.236 ± 0.041
8.991 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.941 ± 0.022
5.385 ± 0.04
3.101 ± 0.025
2.58 ± 0.024
3.963 ± 0.029
6.059 ± 0.033
4.73 ± 0.029
6.495 ± 0.031
0.75 ± 0.013
4.23 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here