Amino acid dipepetide frequency for Bacillus sp. AFS037270

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.135AlaAla: 6.135 ± 0.08
0.642AlaCys: 0.642 ± 0.019
3.323AlaAsp: 3.323 ± 0.045
4.509AlaGlu: 4.509 ± 0.056
3.216AlaPhe: 3.216 ± 0.046
5.617AlaGly: 5.617 ± 0.063
1.215AlaHis: 1.215 ± 0.027
5.756AlaIle: 5.756 ± 0.063
4.736AlaLys: 4.736 ± 0.057
6.983AlaLeu: 6.983 ± 0.068
1.901AlaMet: 1.901 ± 0.035
2.915AlaAsn: 2.915 ± 0.04
2.2AlaPro: 2.2 ± 0.042
2.139AlaGln: 2.139 ± 0.036
2.497AlaArg: 2.497 ± 0.043
4.007AlaSer: 4.007 ± 0.046
3.257AlaThr: 3.257 ± 0.047
5.354AlaVal: 5.354 ± 0.06
0.666AlaTrp: 0.666 ± 0.021
2.246AlaTyr: 2.246 ± 0.038
0.0AlaXaa: 0.0 ± 0.0
Cys
0.457CysAla: 0.457 ± 0.018
0.11CysCys: 0.11 ± 0.008
0.386CysAsp: 0.386 ± 0.015
0.51CysGlu: 0.51 ± 0.016
0.378CysPhe: 0.378 ± 0.016
0.711CysGly: 0.711 ± 0.022
0.238CysHis: 0.238 ± 0.015
0.574CysIle: 0.574 ± 0.016
0.398CysLys: 0.398 ± 0.014
0.79CysLeu: 0.79 ± 0.022
0.206CysMet: 0.206 ± 0.01
0.336CysAsn: 0.336 ± 0.014
0.373CysPro: 0.373 ± 0.014
0.262CysGln: 0.262 ± 0.011
0.313CysArg: 0.313 ± 0.014
0.575CysSer: 0.575 ± 0.018
0.407CysThr: 0.407 ± 0.015
0.453CysVal: 0.453 ± 0.015
0.094CysTrp: 0.094 ± 0.008
0.28CysTyr: 0.28 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
3.035AspAla: 3.035 ± 0.038
0.397AspCys: 0.397 ± 0.015
2.136AspAsp: 2.136 ± 0.038
3.69AspGlu: 3.69 ± 0.047
2.472AspPhe: 2.472 ± 0.04
3.291AspGly: 3.291 ± 0.05
1.112AspHis: 1.112 ± 0.025
3.878AspIle: 3.878 ± 0.05
3.011AspLys: 3.011 ± 0.047
4.973AspLeu: 4.973 ± 0.058
1.231AspMet: 1.231 ± 0.025
1.773AspAsn: 1.773 ± 0.036
2.018AspPro: 2.018 ± 0.034
2.021AspGln: 2.021 ± 0.038
2.153AspArg: 2.153 ± 0.035
2.631AspSer: 2.631 ± 0.039
2.391AspThr: 2.391 ± 0.04
3.486AspVal: 3.486 ± 0.048
0.692AspTrp: 0.692 ± 0.021
2.136AspTyr: 2.136 ± 0.034
0.001AspXaa: 0.001 ± 0.0
Glu
4.867GluAla: 4.867 ± 0.059
0.379GluCys: 0.379 ± 0.016
3.309GluAsp: 3.309 ± 0.049
6.039GluGlu: 6.039 ± 0.076
2.661GluPhe: 2.661 ± 0.043
4.112GluGly: 4.112 ± 0.045
1.445GluHis: 1.445 ± 0.027
5.535GluIle: 5.535 ± 0.065
6.309GluLys: 6.309 ± 0.062
6.873GluLeu: 6.873 ± 0.071
2.098GluMet: 2.098 ± 0.035
3.594GluAsn: 3.594 ± 0.047
1.869GluPro: 1.869 ± 0.03
3.058GluGln: 3.058 ± 0.048
3.095GluArg: 3.095 ± 0.049
3.297GluSer: 3.297 ± 0.042
3.704GluThr: 3.704 ± 0.051
4.774GluVal: 4.774 ± 0.061
0.84GluTrp: 0.84 ± 0.022
2.232GluTyr: 2.232 ± 0.035
0.0GluXaa: 0.0 ± 0.0
Phe
3.121PheAla: 3.121 ± 0.048
0.4PheCys: 0.4 ± 0.015
2.299PheAsp: 2.299 ± 0.036
2.807PheGlu: 2.807 ± 0.038
2.48PhePhe: 2.48 ± 0.05
3.589PheGly: 3.589 ± 0.051
1.087PheHis: 1.087 ± 0.027
4.033PheIle: 4.033 ± 0.058
2.604PheLys: 2.604 ± 0.034
4.82PheLeu: 4.82 ± 0.072
1.186PheMet: 1.186 ± 0.026
2.045PheAsn: 2.045 ± 0.035
1.848PhePro: 1.848 ± 0.033
1.576PheGln: 1.576 ± 0.029
1.578PheArg: 1.578 ± 0.03
3.362PheSer: 3.362 ± 0.048
2.679PheThr: 2.679 ± 0.041
3.125PheVal: 3.125 ± 0.044
0.536PheTrp: 0.536 ± 0.017
1.787PheTyr: 1.787 ± 0.036
0.0PheXaa: 0.0 ± 0.0
Gly
4.981GlyAla: 4.981 ± 0.056
0.717GlyCys: 0.717 ± 0.022
3.054GlyAsp: 3.054 ± 0.037
4.267GlyGlu: 4.267 ± 0.049
3.64GlyPhe: 3.64 ± 0.053
5.111GlyGly: 5.111 ± 0.07
1.467GlyHis: 1.467 ± 0.032
6.381GlyIle: 6.381 ± 0.074
5.275GlyLys: 5.275 ± 0.065
6.967GlyLeu: 6.967 ± 0.072
2.125GlyMet: 2.125 ± 0.035
2.951GlyAsn: 2.951 ± 0.047
1.903GlyPro: 1.903 ± 0.036
2.228GlyGln: 2.228 ± 0.036
2.675GlyArg: 2.675 ± 0.035
4.165GlySer: 4.165 ± 0.052
4.223GlyThr: 4.223 ± 0.052
5.162GlyVal: 5.162 ± 0.059
0.945GlyTrp: 0.945 ± 0.032
2.904GlyTyr: 2.904 ± 0.043
0.0GlyXaa: 0.0 ± 0.0
His
1.222HisAla: 1.222 ± 0.029
0.197HisCys: 0.197 ± 0.01
0.991HisAsp: 0.991 ± 0.026
1.293HisGlu: 1.293 ± 0.024
1.129HisPhe: 1.129 ± 0.028
1.46HisGly: 1.46 ± 0.031
0.67HisHis: 0.67 ± 0.022
1.493HisIle: 1.493 ± 0.029
1.055HisLys: 1.055 ± 0.02
2.137HisLeu: 2.137 ± 0.037
0.476HisMet: 0.476 ± 0.018
0.814HisAsn: 0.814 ± 0.022
1.166HisPro: 1.166 ± 0.026
0.904HisGln: 0.904 ± 0.022
0.814HisArg: 0.814 ± 0.022
1.243HisSer: 1.243 ± 0.028
1.03HisThr: 1.03 ± 0.024
1.373HisVal: 1.373 ± 0.026
0.259HisTrp: 0.259 ± 0.013
0.903HisTyr: 0.903 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
5.921IleAla: 5.921 ± 0.069
0.733IleCys: 0.733 ± 0.021
4.057IleAsp: 4.057 ± 0.051
5.463IleGlu: 5.463 ± 0.063
3.556IlePhe: 3.556 ± 0.055
6.083IleGly: 6.083 ± 0.061
1.732IleHis: 1.732 ± 0.032
6.129IleIle: 6.129 ± 0.073
4.961IleLys: 4.961 ± 0.059
7.703IleLeu: 7.703 ± 0.08
1.905IleMet: 1.905 ± 0.038
3.732IleAsn: 3.732 ± 0.052
3.586IlePro: 3.586 ± 0.052
2.811IleGln: 2.811 ± 0.038
3.057IleArg: 3.057 ± 0.04
5.228IleSer: 5.228 ± 0.061
4.49IleThr: 4.49 ± 0.05
5.426IleVal: 5.426 ± 0.063
0.717IleTrp: 0.717 ± 0.021
2.552IleTyr: 2.552 ± 0.035
0.0IleXaa: 0.0 ± 0.0
Lys
4.691LysAla: 4.691 ± 0.06
0.355LysCys: 0.355 ± 0.017
3.779LysAsp: 3.779 ± 0.054
6.459LysGlu: 6.459 ± 0.062
2.11LysPhe: 2.11 ± 0.032
4.686LysGly: 4.686 ± 0.053
1.268LysHis: 1.268 ± 0.026
5.012LysIle: 5.012 ± 0.053
5.822LysLys: 5.822 ± 0.063
5.947LysLeu: 5.947 ± 0.057
2.204LysMet: 2.204 ± 0.04
3.645LysAsn: 3.645 ± 0.048
2.35LysPro: 2.35 ± 0.039
2.996LysGln: 2.996 ± 0.043
3.065LysArg: 3.065 ± 0.045
3.586LysSer: 3.586 ± 0.042
3.719LysThr: 3.719 ± 0.046
4.877LysVal: 4.877 ± 0.054
0.876LysTrp: 0.876 ± 0.021
2.275LysTyr: 2.275 ± 0.034
0.0LysXaa: 0.0 ± 0.0
Leu
7.257LeuAla: 7.257 ± 0.067
0.734LeuCys: 0.734 ± 0.02
4.647LeuAsp: 4.647 ± 0.052
6.439LeuGlu: 6.439 ± 0.063
4.928LeuPhe: 4.928 ± 0.066
6.786LeuGly: 6.786 ± 0.071
1.849LeuHis: 1.849 ± 0.034
7.509LeuIle: 7.509 ± 0.085
6.922LeuLys: 6.922 ± 0.068
10.164LeuLeu: 10.164 ± 0.102
2.541LeuMet: 2.541 ± 0.04
4.516LeuAsn: 4.516 ± 0.055
3.992LeuPro: 3.992 ± 0.041
3.469LeuGln: 3.469 ± 0.046
3.599LeuArg: 3.599 ± 0.051
6.835LeuSer: 6.835 ± 0.07
5.774LeuThr: 5.774 ± 0.057
6.54LeuVal: 6.54 ± 0.065
0.92LeuTrp: 0.92 ± 0.024
3.108LeuTyr: 3.108 ± 0.047
0.0LeuXaa: 0.0 ± 0.0
Met
2.085MetAla: 2.085 ± 0.035
0.156MetCys: 0.156 ± 0.01
1.429MetAsp: 1.429 ± 0.029
1.922MetGlu: 1.922 ± 0.036
1.077MetPhe: 1.077 ± 0.026
1.964MetGly: 1.964 ± 0.036
0.432MetHis: 0.432 ± 0.015
2.184MetIle: 2.184 ± 0.041
2.286MetLys: 2.286 ± 0.035
2.391MetLeu: 2.391 ± 0.035
0.808MetMet: 0.808 ± 0.021
1.479MetAsn: 1.479 ± 0.031
1.001MetPro: 1.001 ± 0.023
0.809MetGln: 0.809 ± 0.023
0.97MetArg: 0.97 ± 0.021
1.612MetSer: 1.612 ± 0.031
1.498MetThr: 1.498 ± 0.033
1.931MetVal: 1.931 ± 0.033
0.182MetTrp: 0.182 ± 0.01
0.706MetTyr: 0.706 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
2.745AsnAla: 2.745 ± 0.046
0.364AsnCys: 0.364 ± 0.015
2.213AsnAsp: 2.213 ± 0.035
3.315AsnGlu: 3.315 ± 0.059
1.842AsnPhe: 1.842 ± 0.033
3.608AsnGly: 3.608 ± 0.053
1.156AsnHis: 1.156 ± 0.027
3.522AsnIle: 3.522 ± 0.042
3.131AsnLys: 3.131 ± 0.044
4.214AsnLeu: 4.214 ± 0.05
1.217AsnMet: 1.217 ± 0.029
2.19AsnAsn: 2.19 ± 0.045
2.4AsnPro: 2.4 ± 0.039
2.205AsnGln: 2.205 ± 0.035
2.077AsnArg: 2.077 ± 0.037
2.622AsnSer: 2.622 ± 0.043
2.325AsnThr: 2.325 ± 0.041
2.95AsnVal: 2.95 ± 0.04
0.625AsnTrp: 0.625 ± 0.021
1.68AsnTyr: 1.68 ± 0.034
0.0AsnXaa: 0.0 ± 0.0
Pro
2.523ProAla: 2.523 ± 0.046
0.24ProCys: 0.24 ± 0.011
2.077ProAsp: 2.077 ± 0.034
3.027ProGlu: 3.027 ± 0.047
2.121ProPhe: 2.121 ± 0.031
2.462ProGly: 2.462 ± 0.039
0.791ProHis: 0.791 ± 0.021
3.022ProIle: 3.022 ± 0.039
2.295ProLys: 2.295 ± 0.033
3.613ProLeu: 3.613 ± 0.048
0.913ProMet: 0.913 ± 0.022
1.898ProAsn: 1.898 ± 0.034
0.989ProPro: 0.989 ± 0.028
1.129ProGln: 1.129 ± 0.029
1.144ProArg: 1.144 ± 0.023
2.355ProSer: 2.355 ± 0.041
1.989ProThr: 1.989 ± 0.036
2.896ProVal: 2.896 ± 0.042
0.457ProTrp: 0.457 ± 0.018
1.487ProTyr: 1.487 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
2.566GlnAla: 2.566 ± 0.042
0.218GlnCys: 0.218 ± 0.011
1.61GlnAsp: 1.61 ± 0.033
2.638GlnGlu: 2.638 ± 0.041
1.626GlnPhe: 1.626 ± 0.031
2.183GlnGly: 2.183 ± 0.037
0.737GlnHis: 0.737 ± 0.02
2.702GlnIle: 2.702 ± 0.038
2.778GlnLys: 2.778 ± 0.04
3.848GlnLeu: 3.848 ± 0.052
1.087GlnMet: 1.087 ± 0.025
1.771GlnAsn: 1.771 ± 0.036
1.244GlnPro: 1.244 ± 0.026
1.658GlnGln: 1.658 ± 0.038
1.376GlnArg: 1.376 ± 0.029
2.088GlnSer: 2.088 ± 0.037
2.062GlnThr: 2.062 ± 0.036
2.464GlnVal: 2.464 ± 0.036
0.427GlnTrp: 0.427 ± 0.016
1.381GlnTyr: 1.381 ± 0.028
0.001GlnXaa: 0.001 ± 0.001
Arg
2.335ArgAla: 2.335 ± 0.039
0.264ArgCys: 0.264 ± 0.012
1.954ArgAsp: 1.954 ± 0.035
2.959ArgGlu: 2.959 ± 0.044
1.913ArgPhe: 1.913 ± 0.031
2.345ArgGly: 2.345 ± 0.041
0.739ArgHis: 0.739 ± 0.019
3.098ArgIle: 3.098 ± 0.043
3.052ArgLys: 3.052 ± 0.041
3.905ArgLeu: 3.905 ± 0.053
1.153ArgMet: 1.153 ± 0.022
1.958ArgAsn: 1.958 ± 0.03
1.337ArgPro: 1.337 ± 0.027
1.418ArgGln: 1.418 ± 0.031
1.732ArgArg: 1.732 ± 0.034
2.131ArgSer: 2.131 ± 0.038
1.98ArgThr: 1.98 ± 0.034
2.604ArgVal: 2.604 ± 0.043
0.448ArgTrp: 0.448 ± 0.016
1.517ArgTyr: 1.517 ± 0.031
0.0ArgXaa: 0.0 ± 0.0
Ser
3.78SerAla: 3.78 ± 0.047
0.482SerCys: 0.482 ± 0.015
2.715SerAsp: 2.715 ± 0.043
3.743SerGlu: 3.743 ± 0.051
3.434SerPhe: 3.434 ± 0.048
4.597SerGly: 4.597 ± 0.055
1.267SerHis: 1.267 ± 0.024
5.113SerIle: 5.113 ± 0.06
3.955SerLys: 3.955 ± 0.047
6.29SerLeu: 6.29 ± 0.066
1.68SerMet: 1.68 ± 0.031
2.674SerAsn: 2.674 ± 0.044
2.237SerPro: 2.237 ± 0.032
1.985SerGln: 1.985 ± 0.035
2.271SerArg: 2.271 ± 0.032
4.113SerSer: 4.113 ± 0.057
3.267SerThr: 3.267 ± 0.052
4.105SerVal: 4.105 ± 0.051
0.704SerTrp: 0.704 ± 0.023
2.277SerTyr: 2.277 ± 0.032
0.0SerXaa: 0.0 ± 0.0
Thr
4.087ThrAla: 4.087 ± 0.056
0.418ThrCys: 0.418 ± 0.016
2.638ThrAsp: 2.638 ± 0.043
3.358ThrGlu: 3.358 ± 0.044
2.625ThrPhe: 2.625 ± 0.042
4.405ThrGly: 4.405 ± 0.055
1.02ThrHis: 1.02 ± 0.021
4.694ThrIle: 4.694 ± 0.056
3.423ThrLys: 3.423 ± 0.042
5.193ThrLeu: 5.193 ± 0.057
1.233ThrMet: 1.233 ± 0.024
2.616ThrAsn: 2.616 ± 0.042
2.361ThrPro: 2.361 ± 0.033
1.4ThrGln: 1.4 ± 0.029
1.804ThrArg: 1.804 ± 0.03
3.387ThrSer: 3.387 ± 0.043
3.014ThrThr: 3.014 ± 0.043
4.175ThrVal: 4.175 ± 0.057
0.598ThrTrp: 0.598 ± 0.02
1.879ThrTyr: 1.879 ± 0.033
0.0ThrXaa: 0.0 ± 0.0
Val
4.732ValAla: 4.732 ± 0.058
0.626ValCys: 0.626 ± 0.019
3.505ValAsp: 3.505 ± 0.043
4.632ValGlu: 4.632 ± 0.057
3.281ValPhe: 3.281 ± 0.049
4.741ValGly: 4.741 ± 0.053
1.313ValHis: 1.313 ± 0.028
5.836ValIle: 5.836 ± 0.062
4.768ValLys: 4.768 ± 0.058
6.779ValLeu: 6.779 ± 0.074
1.76ValMet: 1.76 ± 0.034
3.309ValAsn: 3.309 ± 0.045
2.805ValPro: 2.805 ± 0.042
2.306ValGln: 2.306 ± 0.038
2.575ValArg: 2.575 ± 0.043
4.556ValSer: 4.556 ± 0.058
4.16ValThr: 4.16 ± 0.048
4.932ValVal: 4.932 ± 0.068
0.69ValTrp: 0.69 ± 0.019
2.292ValTyr: 2.292 ± 0.032
0.0ValXaa: 0.0 ± 0.0
Trp
0.7TrpAla: 0.7 ± 0.022
0.088TrpCys: 0.088 ± 0.008
0.601TrpAsp: 0.601 ± 0.018
0.706TrpGlu: 0.706 ± 0.02
0.575TrpPhe: 0.575 ± 0.021
0.816TrpGly: 0.816 ± 0.028
0.224TrpHis: 0.224 ± 0.011
0.878TrpIle: 0.878 ± 0.021
0.825TrpLys: 0.825 ± 0.022
1.201TrpLeu: 1.201 ± 0.033
0.355TrpMet: 0.355 ± 0.016
0.631TrpAsn: 0.631 ± 0.019
0.275TrpPro: 0.275 ± 0.014
0.408TrpGln: 0.408 ± 0.015
0.451TrpArg: 0.451 ± 0.015
0.68TrpSer: 0.68 ± 0.021
0.534TrpThr: 0.534 ± 0.019
0.767TrpVal: 0.767 ± 0.022
0.162TrpTrp: 0.162 ± 0.01
0.389TrpTyr: 0.389 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.076TyrAla: 2.076 ± 0.033
0.334TyrCys: 0.334 ± 0.014
1.799TyrAsp: 1.799 ± 0.035
2.313TyrGlu: 2.313 ± 0.039
1.898TyrPhe: 1.898 ± 0.039
2.57TyrGly: 2.57 ± 0.04
0.869TyrHis: 0.869 ± 0.024
2.492TyrIle: 2.492 ± 0.04
2.118TyrLys: 2.118 ± 0.037
3.737TyrLeu: 3.737 ± 0.053
0.842TyrMet: 0.842 ± 0.022
1.555TyrAsn: 1.555 ± 0.032
1.475TyrPro: 1.475 ± 0.029
1.684TyrGln: 1.684 ± 0.029
1.598TyrArg: 1.598 ± 0.031
2.216TyrSer: 2.216 ± 0.034
1.822TyrThr: 1.822 ± 0.029
2.203TyrVal: 2.203 ± 0.033
0.429TyrTrp: 0.429 ± 0.014
1.432TyrTyr: 1.432 ± 0.031
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.001XaaPro: 0.001 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.001XaaSer: 0.001 ± 0.001
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5980 proteins (1805640 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski