Sulfurospirillum sp. UBA12182
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2326 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D3W5W6|A0A2D3W5W6_9PROT Ketol-acid reductoisomerase (NADP(+)) OS=Sulfurospirillum sp. UBA12182 OX=2015905 GN=ilvC PE=3 SV=1
MM1 pKa = 6.89 NTSFYY6 pKa = 11.29 NGISGIKK13 pKa = 9.1 SHH15 pKa = 6.71 QFGLDD20 pKa = 2.82 VWADD24 pKa = 3.65 NISNISTIGYY34 pKa = 8.71 KK35 pKa = 10.0 AANPEE40 pKa = 3.8 FSTIFSTTLANSYY53 pKa = 11.22 SDD55 pKa = 3.41 PTSNNQGLGSRR66 pKa = 11.84 AQATTLNMSQGIFQNTDD83 pKa = 2.46 NVFDD87 pKa = 4.58 LSIGGEE93 pKa = 4.17 GWFGVQSLEE102 pKa = 3.77 NQTYY106 pKa = 6.17 YY107 pKa = 10.49 TRR109 pKa = 11.84 AGSFSMDD116 pKa = 2.9 TDD118 pKa = 4.27 GYY120 pKa = 11.44 LVDD123 pKa = 5.15 AYY125 pKa = 10.88 GNYY128 pKa = 10.74 LLGTLGGNVTPTSLSDD144 pKa = 3.87 RR145 pKa = 11.84 ILNEE149 pKa = 3.24 FGTYY153 pKa = 10.15 YY154 pKa = 10.86 KK155 pKa = 10.74 VGNTQEE161 pKa = 4.85 LGTPYY166 pKa = 10.25 SISALEE172 pKa = 5.19 DD173 pKa = 2.97 ITLSSVEE180 pKa = 4.21 SQSKK184 pKa = 10.52 INLPDD189 pKa = 3.1 ILYY192 pKa = 9.28 FPPEE196 pKa = 3.35 ATTYY200 pKa = 10.94 VKK202 pKa = 10.79 YY203 pKa = 10.09 QANLNPKK210 pKa = 9.57 INIGSTKK217 pKa = 9.69 IDD219 pKa = 4.97 LDD221 pKa = 3.74 TLDD224 pKa = 4.39 IGTPAIDD231 pKa = 3.85 TVNEE235 pKa = 4.1 TLSISGTVSNTTALQNPQSGDD256 pKa = 3.6 VVILTVTDD264 pKa = 3.72 EE265 pKa = 4.53 NGNSVDD271 pKa = 3.09 ITAEE275 pKa = 4.05 LTPLSSTDD283 pKa = 3.28 STLVWSFSNSDD294 pKa = 3.08 ISDD297 pKa = 4.23 LDD299 pKa = 3.66 TSTSLTITAQLQTEE313 pKa = 4.16 QEE315 pKa = 4.24 IPNVEE320 pKa = 4.16 HH321 pKa = 6.45 FTTEE325 pKa = 3.86 IISPEE330 pKa = 4.09 GEE332 pKa = 3.83 KK333 pKa = 11.03 DD334 pKa = 3.93 FIDD337 pKa = 3.3 MTFTKK342 pKa = 10.22 RR343 pKa = 11.84 VPQSALEE350 pKa = 4.36 TTWDD354 pKa = 3.46 AQIQILSFYY363 pKa = 9.62 EE364 pKa = 4.19 DD365 pKa = 4.05 YY366 pKa = 11.15 VIQTYY371 pKa = 10.43 DD372 pKa = 3.19 PSVTYY377 pKa = 10.63 DD378 pKa = 3.22 PTLYY382 pKa = 10.65 NVNLEE387 pKa = 4.4 TNQVTKK393 pKa = 10.35 IYY395 pKa = 10.79 DD396 pKa = 3.35 PTLYY400 pKa = 10.71 KK401 pKa = 10.38 VDD403 pKa = 3.84 LGTNKK408 pKa = 9.45 VYY410 pKa = 10.66 EE411 pKa = 5.04 IIDD414 pKa = 3.86 SQIGDD419 pKa = 3.59 ATFAGAGEE427 pKa = 5.05 LIASNMPTLSNGGTPLSINIGTPYY451 pKa = 10.73 AEE453 pKa = 4.29 EE454 pKa = 4.05 NGVIISNGYY463 pKa = 10.4 DD464 pKa = 3.17 GMVSHH469 pKa = 7.34 VDD471 pKa = 3.35 LDD473 pKa = 3.54 KK474 pKa = 11.45 ARR476 pKa = 11.84 YY477 pKa = 8.25 SEE479 pKa = 3.9 KK480 pKa = 10.62 DD481 pKa = 3.35 GYY483 pKa = 11.53 VEE485 pKa = 4.64 GLLKK489 pKa = 10.57 AYY491 pKa = 10.29 GMDD494 pKa = 3.08 GRR496 pKa = 11.84 GNVIAEE502 pKa = 4.09 FTNGRR507 pKa = 11.84 SAPIAKK513 pKa = 9.41 IALYY517 pKa = 10.65 HH518 pKa = 6.2 FMNDD522 pKa = 2.92 QGLEE526 pKa = 4.28 KK527 pKa = 10.74 VSSTLFSASANSGDD541 pKa = 4.12 AIFYY545 pKa = 10.09 TDD547 pKa = 4.14 ANGNPILGSAIYY559 pKa = 10.34 SNRR562 pKa = 11.84 LEE564 pKa = 4.21 GSNVSMATALTEE576 pKa = 4.51 LIVMQKK582 pKa = 10.76 AFDD585 pKa = 3.92 ASAKK589 pKa = 10.62 SITTSDD595 pKa = 3.52 QMIQNAINMKK605 pKa = 10.24 AA606 pKa = 3.04
Molecular weight: 65.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.756
IPC2_protein 3.973
IPC_protein 3.986
Toseland 3.77
ProMoST 4.126
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.884
Rodwell 3.808
Grimsley 3.681
Solomon 3.961
Lehninger 3.923
Nozaki 4.075
DTASelect 4.304
Thurlkill 3.808
EMBOSS 3.897
Sillero 4.101
Patrickios 1.469
IPC_peptide 3.961
IPC2_peptide 4.088
IPC2.peptide.svr19 3.986
Protein with the highest isoelectric point:
>tr|A0A2D3W5G9|A0A2D3W5G9_9PROT TonB-dependent receptor OS=Sulfurospirillum sp. UBA12182 OX=2015905 GN=CFH82_10390 PE=3 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.04 QPHH8 pKa = 4.86 NTPRR12 pKa = 11.84 KK13 pKa = 7.31 RR14 pKa = 11.84 THH16 pKa = 5.88 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MKK23 pKa = 8.63 TKK25 pKa = 9.65 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VIAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.76 GRR39 pKa = 11.84 KK40 pKa = 8.68 RR41 pKa = 11.84 LSVV44 pKa = 3.2
Molecular weight: 5.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2326
0
2326
698452
20
1506
300.3
33.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.56 ± 0.048
1.016 ± 0.021
5.309 ± 0.036
7.507 ± 0.053
5.389 ± 0.044
5.854 ± 0.053
1.857 ± 0.022
8.508 ± 0.047
8.79 ± 0.053
10.23 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.446 ± 0.023
4.96 ± 0.045
2.97 ± 0.03
3.174 ± 0.027
3.301 ± 0.034
6.628 ± 0.041
4.722 ± 0.034
6.227 ± 0.042
0.726 ± 0.016
3.827 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here