Aureitalea sp. RR4-38
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2826 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S0HXP2|A0A2S0HXP2_9FLAO Uncharacterized protein OS=Aureitalea sp. RR4-38 OX=2094025 GN=C5O00_09530 PE=4 SV=1
MM1 pKa = 7.76 KK2 pKa = 10.26 ILIPSVLQVVLLFNFALIYY21 pKa = 10.51 GQAQFEE27 pKa = 4.34 NQAEE31 pKa = 4.3 SLGAGIAYY39 pKa = 10.29 GNIQLGAGVSFFDD52 pKa = 4.32 YY53 pKa = 11.33 NNDD56 pKa = 3.41 GLDD59 pKa = 4.84 DD60 pKa = 3.67 ITIPASNSRR69 pKa = 11.84 NFTFLKK75 pKa = 10.91 NMGGTFQEE83 pKa = 4.4 EE84 pKa = 4.16 NLGIDD89 pKa = 3.4 SNGFQSRR96 pKa = 11.84 QAMWVDD102 pKa = 4.15 FDD104 pKa = 4.93 NDD106 pKa = 3.21 GDD108 pKa = 3.93 NDD110 pKa = 3.82 FFATSDD116 pKa = 3.68 QDD118 pKa = 3.24 WSRR121 pKa = 11.84 LYY123 pKa = 10.81 QNNGDD128 pKa = 3.9 GTFTDD133 pKa = 3.16 ITLAAGLPTVIYY145 pKa = 8.37 DD146 pKa = 3.81 TYY148 pKa = 10.11 GAAWGDD154 pKa = 4.01 FNNDD158 pKa = 2.18 RR159 pKa = 11.84 HH160 pKa = 7.45 LDD162 pKa = 3.5 VFYY165 pKa = 10.87 SIRR168 pKa = 11.84 DD169 pKa = 3.55 EE170 pKa = 4.1 NQIIPNILYY179 pKa = 10.31 QNNGDD184 pKa = 3.78 GTFTNVTIAAGLEE197 pKa = 4.14 TTGYY201 pKa = 11.15 LSFCASFFDD210 pKa = 3.8 MDD212 pKa = 4.36 KK213 pKa = 11.5 DD214 pKa = 3.82 GDD216 pKa = 3.61 QDD218 pKa = 5.11 LYY220 pKa = 11.14 VANDD224 pKa = 3.93 KK225 pKa = 11.05 YY226 pKa = 11.4 DD227 pKa = 3.71 SPNLMYY233 pKa = 10.67 RR234 pKa = 11.84 NNGDD238 pKa = 3.44 GTFTNVSASSGTGIEE253 pKa = 3.94 IDD255 pKa = 4.26 AMSTTIGDD263 pKa = 3.88 YY264 pKa = 11.56 NNDD267 pKa = 3.25 GWLDD271 pKa = 3.34 IYY273 pKa = 9.74 VTNTEE278 pKa = 4.37 FTPPPPGVNGNVLFRR293 pKa = 11.84 NNADD297 pKa = 3.39 GTFTNVAASTGTIFNSVGWGAVFLDD322 pKa = 4.02 GDD324 pKa = 3.96 NEE326 pKa = 4.46 GFTDD330 pKa = 4.96 LYY332 pKa = 11.04 VSGEE336 pKa = 4.17 FEE338 pKa = 4.18 VDD340 pKa = 3.31 PVYY343 pKa = 10.83 LPSAYY348 pKa = 10.4 YY349 pKa = 10.22 EE350 pKa = 4.11 NQGDD354 pKa = 4.0 HH355 pKa = 6.22 TFVIPTGIGFEE366 pKa = 3.99 NDD368 pKa = 2.76 TRR370 pKa = 11.84 QSYY373 pKa = 10.96 GNAIGDD379 pKa = 4.04 IDD381 pKa = 4.27 NDD383 pKa = 4.45 GYY385 pKa = 10.47 PDD387 pKa = 3.25 IVVMNNNDD395 pKa = 3.26 QNIFLWKK402 pKa = 8.38 NTSIQNHH409 pKa = 5.04 NWLKK413 pKa = 10.86 VKK415 pKa = 11.08 LEE417 pKa = 3.99 GTQGNRR423 pKa = 11.84 MGIGSFIEE431 pKa = 4.01 VGTTHH436 pKa = 6.73 GTQLQFTVCGEE447 pKa = 4.37 GYY449 pKa = 10.46 LGQNSAYY456 pKa = 10.11 EE457 pKa = 4.1 FFGLNDD463 pKa = 3.41 ATNVDD468 pKa = 4.85 FVRR471 pKa = 11.84 VTWPSGIVDD480 pKa = 3.76 YY481 pKa = 11.46 FEE483 pKa = 5.25 DD484 pKa = 4.37 LNVNQHH490 pKa = 4.36 ITIIEE495 pKa = 4.19 GTGILSSDD503 pKa = 3.57 SASFAVFSMYY513 pKa = 10.41 PNPASEE519 pKa = 4.77 LVFLNFPSEE528 pKa = 4.25 AEE530 pKa = 4.04 RR531 pKa = 11.84 QLVVLDD537 pKa = 3.52 VTGKK541 pKa = 9.93 VVISEE546 pKa = 4.45 EE547 pKa = 4.05 IFGNTHH553 pKa = 6.05 QMDD556 pKa = 4.49 LSVISGGVYY565 pKa = 9.11 IVEE568 pKa = 3.98 VRR570 pKa = 11.84 FNGNVHH576 pKa = 6.09 RR577 pKa = 11.84 KK578 pKa = 9.71 KK579 pKa = 10.65 LIKK582 pKa = 10.15 HH583 pKa = 5.84
Molecular weight: 64.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.706
IPC2_protein 3.859
IPC_protein 3.897
Toseland 3.668
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.821
Rodwell 3.719
Grimsley 3.567
Solomon 3.884
Lehninger 3.846
Nozaki 3.999
DTASelect 4.266
Thurlkill 3.719
EMBOSS 3.834
Sillero 4.012
Patrickios 1.202
IPC_peptide 3.884
IPC2_peptide 3.986
IPC2.peptide.svr19 3.894
Protein with the highest isoelectric point:
>tr|A0A2S0HTP0|A0A2S0HTP0_9FLAO DNA gyrase subunit B OS=Aureitalea sp. RR4-38 OX=2094025 GN=gyrB PE=3 SV=1
MM1 pKa = 7.46 SFNSASFKK9 pKa = 10.55 AASLRR14 pKa = 11.84 ANSFIAASFSAASFIAASFNAASFNAASFKK44 pKa = 10.65 AASLRR49 pKa = 11.84 ANSFNAASFRR59 pKa = 11.84 AASLRR64 pKa = 11.84 AASFMAASFKK74 pKa = 10.78 AASFKK79 pKa = 10.77 AASLRR84 pKa = 11.84 AASFF88 pKa = 3.37
Molecular weight: 8.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.406
IPC2_protein 10.877
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.398
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.149
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.042
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2826
0
2826
970976
38
4750
343.6
38.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.757 ± 0.041
0.762 ± 0.017
5.857 ± 0.057
6.83 ± 0.053
5.045 ± 0.037
6.828 ± 0.052
1.79 ± 0.025
7.438 ± 0.039
6.686 ± 0.076
9.28 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.329 ± 0.024
5.63 ± 0.044
3.687 ± 0.035
3.264 ± 0.024
4.003 ± 0.042
6.401 ± 0.035
5.842 ± 0.073
6.493 ± 0.043
1.075 ± 0.017
4.002 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here