Amino acid dipepetide frequency for Aureitalea sp. RR4-38

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.075AlaAla: 5.075 ± 0.104
0.588AlaCys: 0.588 ± 0.029
3.544AlaAsp: 3.544 ± 0.071
4.422AlaGlu: 4.422 ± 0.076
3.289AlaPhe: 3.289 ± 0.052
4.826AlaGly: 4.826 ± 0.093
1.167AlaHis: 1.167 ± 0.038
5.43AlaIle: 5.43 ± 0.085
4.134AlaLys: 4.134 ± 0.08
6.36AlaLeu: 6.36 ± 0.1
1.776AlaMet: 1.776 ± 0.048
3.581AlaAsn: 3.581 ± 0.068
2.023AlaPro: 2.023 ± 0.053
2.534AlaGln: 2.534 ± 0.061
2.619AlaArg: 2.619 ± 0.053
4.329AlaSer: 4.329 ± 0.075
3.993AlaThr: 3.993 ± 0.107
4.673AlaVal: 4.673 ± 0.07
0.698AlaTrp: 0.698 ± 0.047
2.511AlaTyr: 2.511 ± 0.062
0.0AlaXaa: 0.0 ± 0.0
Cys
0.463CysAla: 0.463 ± 0.022
0.107CysCys: 0.107 ± 0.011
0.595CysAsp: 0.595 ± 0.099
0.506CysGlu: 0.506 ± 0.024
0.375CysPhe: 0.375 ± 0.023
0.642CysGly: 0.642 ± 0.032
0.188CysHis: 0.188 ± 0.033
0.515CysIle: 0.515 ± 0.025
0.421CysLys: 0.421 ± 0.021
0.617CysLeu: 0.617 ± 0.027
0.17CysMet: 0.17 ± 0.014
0.39CysAsn: 0.39 ± 0.022
0.337CysPro: 0.337 ± 0.026
0.203CysGln: 0.203 ± 0.032
0.242CysArg: 0.242 ± 0.018
0.557CysSer: 0.557 ± 0.035
0.46CysThr: 0.46 ± 0.027
0.468CysVal: 0.468 ± 0.026
0.068CysTrp: 0.068 ± 0.009
0.298CysTyr: 0.298 ± 0.02
0.0CysXaa: 0.0 ± 0.0
Asp
3.915AspAla: 3.915 ± 0.071
0.495AspCys: 0.495 ± 0.06
3.196AspAsp: 3.196 ± 0.092
3.965AspGlu: 3.965 ± 0.089
3.332AspPhe: 3.332 ± 0.072
4.505AspGly: 4.505 ± 0.195
1.212AspHis: 1.212 ± 0.037
4.398AspIle: 4.398 ± 0.085
3.503AspLys: 3.503 ± 0.073
5.463AspLeu: 5.463 ± 0.075
1.231AspMet: 1.231 ± 0.038
3.1AspAsn: 3.1 ± 0.08
2.805AspPro: 2.805 ± 0.162
1.555AspGln: 1.555 ± 0.039
2.292AspArg: 2.292 ± 0.049
3.404AspSer: 3.404 ± 0.057
3.164AspThr: 3.164 ± 0.101
3.644AspVal: 3.644 ± 0.069
0.728AspTrp: 0.728 ± 0.028
2.664AspTyr: 2.664 ± 0.048
0.0AspXaa: 0.0 ± 0.0
Glu
5.032GluAla: 5.032 ± 0.109
0.349GluCys: 0.349 ± 0.024
3.973GluAsp: 3.973 ± 0.057
5.472GluGlu: 5.472 ± 0.12
3.113GluPhe: 3.113 ± 0.068
4.12GluGly: 4.12 ± 0.069
1.198GluHis: 1.198 ± 0.039
5.717GluIle: 5.717 ± 0.088
5.257GluLys: 5.257 ± 0.112
6.235GluLeu: 6.235 ± 0.106
1.756GluMet: 1.756 ± 0.041
4.287GluAsn: 4.287 ± 0.069
1.698GluPro: 1.698 ± 0.041
2.304GluGln: 2.304 ± 0.048
2.857GluArg: 2.857 ± 0.069
3.316GluSer: 3.316 ± 0.064
3.863GluThr: 3.863 ± 0.072
4.714GluVal: 4.714 ± 0.071
0.628GluTrp: 0.628 ± 0.026
2.413GluTyr: 2.413 ± 0.059
0.0GluXaa: 0.0 ± 0.0
Phe
2.99PheAla: 2.99 ± 0.06
0.388PheCys: 0.388 ± 0.021
3.252PheAsp: 3.252 ± 0.065
3.344PheGlu: 3.344 ± 0.061
2.59PhePhe: 2.59 ± 0.061
3.641PheGly: 3.641 ± 0.072
0.863PheHis: 0.863 ± 0.033
3.625PheIle: 3.625 ± 0.081
3.08PheLys: 3.08 ± 0.069
4.592PheLeu: 4.592 ± 0.084
1.071PheMet: 1.071 ± 0.037
2.942PheAsn: 2.942 ± 0.064
1.81PhePro: 1.81 ± 0.046
1.517PheGln: 1.517 ± 0.041
2.015PheArg: 2.015 ± 0.048
3.774PheSer: 3.774 ± 0.071
3.178PheThr: 3.178 ± 0.069
3.09PheVal: 3.09 ± 0.065
0.563PheTrp: 0.563 ± 0.028
2.123PheTyr: 2.123 ± 0.057
0.0PheXaa: 0.0 ± 0.0
Gly
4.477GlyAla: 4.477 ± 0.08
0.625GlyCys: 0.625 ± 0.036
4.091GlyAsp: 4.091 ± 0.138
4.008GlyGlu: 4.008 ± 0.086
3.632GlyPhe: 3.632 ± 0.068
5.123GlyGly: 5.123 ± 0.123
1.178GlyHis: 1.178 ± 0.04
5.479GlyIle: 5.479 ± 0.094
4.571GlyLys: 4.571 ± 0.088
5.861GlyLeu: 5.861 ± 0.087
1.864GlyMet: 1.864 ± 0.041
3.95GlyAsn: 3.95 ± 0.112
1.716GlyPro: 1.716 ± 0.062
1.924GlyGln: 1.924 ± 0.049
2.466GlyArg: 2.466 ± 0.056
4.518GlySer: 4.518 ± 0.096
4.475GlyThr: 4.475 ± 0.124
4.551GlyVal: 4.551 ± 0.081
0.85GlyTrp: 0.85 ± 0.031
2.921GlyTyr: 2.921 ± 0.065
0.0GlyXaa: 0.0 ± 0.0
His
1.021HisAla: 1.021 ± 0.037
0.157HisCys: 0.157 ± 0.011
0.883HisAsp: 0.883 ± 0.036
1.003HisGlu: 1.003 ± 0.037
1.109HisPhe: 1.109 ± 0.033
1.149HisGly: 1.149 ± 0.038
0.468HisHis: 0.468 ± 0.023
1.362HisIle: 1.362 ± 0.038
1.157HisLys: 1.157 ± 0.039
1.845HisLeu: 1.845 ± 0.047
0.361HisMet: 0.361 ± 0.019
0.91HisAsn: 0.91 ± 0.036
1.001HisPro: 1.001 ± 0.04
0.639HisGln: 0.639 ± 0.028
0.784HisArg: 0.784 ± 0.028
1.052HisSer: 1.052 ± 0.036
1.0HisThr: 1.0 ± 0.034
0.958HisVal: 0.958 ± 0.037
0.207HisTrp: 0.207 ± 0.016
0.84HisTyr: 0.84 ± 0.031
0.0HisXaa: 0.0 ± 0.0
Ile
5.619IleAla: 5.619 ± 0.08
0.596IleCys: 0.596 ± 0.028
4.809IleAsp: 4.809 ± 0.068
5.263IleGlu: 5.263 ± 0.076
3.484IlePhe: 3.484 ± 0.068
4.835IleGly: 4.835 ± 0.09
1.283IleHis: 1.283 ± 0.042
5.154IleIle: 5.154 ± 0.097
4.557IleLys: 4.557 ± 0.086
6.726IleLeu: 6.726 ± 0.109
1.417IleMet: 1.417 ± 0.038
3.835IleAsn: 3.835 ± 0.075
3.301IlePro: 3.301 ± 0.064
2.27IleGln: 2.27 ± 0.052
2.955IleArg: 2.955 ± 0.07
5.617IleSer: 5.617 ± 0.084
4.613IleThr: 4.613 ± 0.09
4.723IleVal: 4.723 ± 0.072
0.671IleTrp: 0.671 ± 0.028
2.646IleTyr: 2.646 ± 0.044
0.0IleXaa: 0.0 ± 0.0
Lys
4.445LysAla: 4.445 ± 0.091
0.322LysCys: 0.322 ± 0.021
4.112LysAsp: 4.112 ± 0.092
5.397LysGlu: 5.397 ± 0.103
2.548LysPhe: 2.548 ± 0.055
4.103LysGly: 4.103 ± 0.094
1.209LysHis: 1.209 ± 0.04
4.928LysIle: 4.928 ± 0.091
5.74LysLys: 5.74 ± 0.121
6.053LysLeu: 6.053 ± 0.095
1.766LysMet: 1.766 ± 0.047
3.858LysAsn: 3.858 ± 0.072
2.197LysPro: 2.197 ± 0.061
2.426LysGln: 2.426 ± 0.058
2.968LysArg: 2.968 ± 0.065
3.636LysSer: 3.636 ± 0.073
3.708LysThr: 3.708 ± 0.076
4.145LysVal: 4.145 ± 0.078
0.676LysTrp: 0.676 ± 0.027
2.621LysTyr: 2.621 ± 0.056
0.0LysXaa: 0.0 ± 0.0
Leu
6.153LeuAla: 6.153 ± 0.094
0.73LeuCys: 0.73 ± 0.044
5.341LeuAsp: 5.341 ± 0.092
6.079LeuGlu: 6.079 ± 0.096
4.83LeuPhe: 4.83 ± 0.083
6.016LeuGly: 6.016 ± 0.09
1.624LeuHis: 1.624 ± 0.043
6.696LeuIle: 6.696 ± 0.1
6.821LeuLys: 6.821 ± 0.112
9.304LeuLeu: 9.304 ± 0.145
2.14LeuMet: 2.14 ± 0.055
5.081LeuAsn: 5.081 ± 0.084
3.639LeuPro: 3.639 ± 0.064
3.336LeuGln: 3.336 ± 0.07
3.771LeuArg: 3.771 ± 0.077
6.607LeuSer: 6.607 ± 0.091
4.837LeuThr: 4.837 ± 0.069
5.712LeuVal: 5.712 ± 0.079
0.874LeuTrp: 0.874 ± 0.036
3.211LeuTyr: 3.211 ± 0.067
0.0LeuXaa: 0.0 ± 0.0
Met
1.816MetAla: 1.816 ± 0.05
0.127MetCys: 0.127 ± 0.012
1.4MetAsp: 1.4 ± 0.04
1.63MetGlu: 1.63 ± 0.049
0.849MetPhe: 0.849 ± 0.034
1.616MetGly: 1.616 ± 0.042
0.433MetHis: 0.433 ± 0.018
1.607MetIle: 1.607 ± 0.045
2.246MetLys: 2.246 ± 0.048
2.054MetLeu: 2.054 ± 0.045
0.583MetMet: 0.583 ± 0.025
1.273MetAsn: 1.273 ± 0.033
0.863MetPro: 0.863 ± 0.035
0.875MetGln: 0.875 ± 0.03
1.008MetArg: 1.008 ± 0.031
1.354MetSer: 1.354 ± 0.037
1.137MetThr: 1.137 ± 0.035
1.497MetVal: 1.497 ± 0.04
0.166MetTrp: 0.166 ± 0.013
0.758MetTyr: 0.758 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
3.716AsnAla: 3.716 ± 0.069
0.469AsnCys: 0.469 ± 0.028
3.36AsnAsp: 3.36 ± 0.11
3.612AsnGlu: 3.612 ± 0.063
2.907AsnPhe: 2.907 ± 0.064
3.99AsnGly: 3.99 ± 0.121
0.819AsnHis: 0.819 ± 0.027
4.077AsnIle: 4.077 ± 0.064
3.441AsnLys: 3.441 ± 0.073
4.789AsnLeu: 4.789 ± 0.077
1.281AsnMet: 1.281 ± 0.04
3.35AsnAsn: 3.35 ± 0.09
2.82AsnPro: 2.82 ± 0.084
1.672AsnGln: 1.672 ± 0.063
2.311AsnArg: 2.311 ± 0.05
3.597AsnSer: 3.597 ± 0.067
3.547AsnThr: 3.547 ± 0.078
3.356AsnVal: 3.356 ± 0.092
0.749AsnTrp: 0.749 ± 0.033
2.442AsnTyr: 2.442 ± 0.051
0.0AsnXaa: 0.0 ± 0.0
Pro
2.412ProAla: 2.412 ± 0.069
0.261ProCys: 0.261 ± 0.033
2.53ProAsp: 2.53 ± 0.085
3.044ProGlu: 3.044 ± 0.056
1.88ProPhe: 1.88 ± 0.041
2.553ProGly: 2.553 ± 0.072
0.678ProHis: 0.678 ± 0.026
2.426ProIle: 2.426 ± 0.05
2.236ProLys: 2.236 ± 0.055
3.197ProLeu: 3.197 ± 0.073
0.83ProMet: 0.83 ± 0.031
2.183ProAsn: 2.183 ± 0.063
1.157ProPro: 1.157 ± 0.056
1.268ProGln: 1.268 ± 0.046
1.165ProArg: 1.165 ± 0.041
2.267ProSer: 2.267 ± 0.049
2.037ProThr: 2.037 ± 0.068
2.913ProVal: 2.913 ± 0.071
0.374ProTrp: 0.374 ± 0.02
1.465ProTyr: 1.465 ± 0.04
0.0ProXaa: 0.0 ± 0.0
Gln
2.226GlnAla: 2.226 ± 0.075
0.236GlnCys: 0.236 ± 0.022
1.645GlnAsp: 1.645 ± 0.047
2.103GlnGlu: 2.103 ± 0.053
1.634GlnPhe: 1.634 ± 0.039
1.916GlnGly: 1.916 ± 0.049
0.597GlnHis: 0.597 ± 0.024
2.319GlnIle: 2.319 ± 0.062
2.316GlnLys: 2.316 ± 0.058
3.666GlnLeu: 3.666 ± 0.076
0.824GlnMet: 0.824 ± 0.031
1.807GlnAsn: 1.807 ± 0.051
1.206GlnPro: 1.206 ± 0.039
1.55GlnGln: 1.55 ± 0.044
1.39GlnArg: 1.39 ± 0.037
1.766GlnSer: 1.766 ± 0.042
1.798GlnThr: 1.798 ± 0.058
1.942GlnVal: 1.942 ± 0.054
0.392GlnTrp: 0.392 ± 0.019
1.305GlnTyr: 1.305 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
2.369ArgAla: 2.369 ± 0.056
0.23ArgCys: 0.23 ± 0.015
2.197ArgAsp: 2.197 ± 0.053
2.762ArgGlu: 2.762 ± 0.065
2.046ArgPhe: 2.046 ± 0.053
2.256ArgGly: 2.256 ± 0.054
0.732ArgHis: 0.732 ± 0.026
3.259ArgIle: 3.259 ± 0.058
3.188ArgLys: 3.188 ± 0.069
3.707ArgLeu: 3.707 ± 0.069
1.084ArgMet: 1.084 ± 0.035
2.356ArgAsn: 2.356 ± 0.052
1.287ArgPro: 1.287 ± 0.044
1.211ArgGln: 1.211 ± 0.037
1.823ArgArg: 1.823 ± 0.049
2.649ArgSer: 2.649 ± 0.051
2.142ArgThr: 2.142 ± 0.049
2.415ArgVal: 2.415 ± 0.053
0.457ArgTrp: 0.457 ± 0.023
1.856ArgTyr: 1.856 ± 0.045
0.0ArgXaa: 0.0 ± 0.0
Ser
3.932SerAla: 3.932 ± 0.065
0.636SerCys: 0.636 ± 0.029
3.327SerAsp: 3.327 ± 0.078
4.855SerGlu: 4.855 ± 0.084
3.681SerPhe: 3.681 ± 0.068
5.149SerGly: 5.149 ± 0.097
1.042SerHis: 1.042 ± 0.034
4.556SerIle: 4.556 ± 0.071
3.833SerLys: 3.833 ± 0.077
6.089SerLeu: 6.089 ± 0.09
1.46SerMet: 1.46 ± 0.043
3.41SerAsn: 3.41 ± 0.07
2.248SerPro: 2.248 ± 0.052
1.936SerGln: 1.936 ± 0.05
2.538SerArg: 2.538 ± 0.057
4.115SerSer: 4.115 ± 0.076
3.577SerThr: 3.577 ± 0.067
4.142SerVal: 4.142 ± 0.065
0.78SerTrp: 0.78 ± 0.032
2.7SerTyr: 2.7 ± 0.071
0.0SerXaa: 0.0 ± 0.0
Thr
4.234ThrAla: 4.234 ± 0.102
0.368ThrCys: 0.368 ± 0.028
3.516ThrAsp: 3.516 ± 0.121
3.66ThrGlu: 3.66 ± 0.071
2.965ThrPhe: 2.965 ± 0.077
4.402ThrGly: 4.402 ± 0.096
1.084ThrHis: 1.084 ± 0.036
4.464ThrIle: 4.464 ± 0.088
2.938ThrLys: 2.938 ± 0.064
5.353ThrLeu: 5.353 ± 0.08
1.072ThrMet: 1.072 ± 0.036
3.192ThrAsn: 3.192 ± 0.114
2.581ThrPro: 2.581 ± 0.09
1.828ThrGln: 1.828 ± 0.057
2.059ThrArg: 2.059 ± 0.05
3.753ThrSer: 3.753 ± 0.073
3.667ThrThr: 3.667 ± 0.12
4.27ThrVal: 4.27 ± 0.143
0.601ThrTrp: 0.601 ± 0.033
2.414ThrTyr: 2.414 ± 0.063
0.0ThrXaa: 0.0 ± 0.0
Val
4.489ValAla: 4.489 ± 0.073
0.544ValCys: 0.544 ± 0.024
3.85ValAsp: 3.85 ± 0.078
4.123ValGlu: 4.123 ± 0.074
3.243ValPhe: 3.243 ± 0.066
4.037ValGly: 4.037 ± 0.071
1.059ValHis: 1.059 ± 0.034
5.04ValIle: 5.04 ± 0.075
4.029ValLys: 4.029 ± 0.073
6.152ValLeu: 6.152 ± 0.097
1.513ValMet: 1.513 ± 0.048
3.598ValAsn: 3.598 ± 0.068
2.391ValPro: 2.391 ± 0.054
1.856ValGln: 1.856 ± 0.041
2.408ValArg: 2.408 ± 0.064
4.54ValSer: 4.54 ± 0.095
4.2ValThr: 4.2 ± 0.159
4.642ValVal: 4.642 ± 0.093
0.645ValTrp: 0.645 ± 0.026
2.572ValTyr: 2.572 ± 0.057
0.0ValXaa: 0.0 ± 0.0
Trp
0.668TrpAla: 0.668 ± 0.029
0.073TrpCys: 0.073 ± 0.01
0.657TrpAsp: 0.657 ± 0.032
0.728TrpGlu: 0.728 ± 0.03
0.574TrpPhe: 0.574 ± 0.025
0.663TrpGly: 0.663 ± 0.029
0.24TrpHis: 0.24 ± 0.019
0.768TrpIle: 0.768 ± 0.03
0.747TrpLys: 0.747 ± 0.031
1.003TrpLeu: 1.003 ± 0.033
0.34TrpMet: 0.34 ± 0.018
0.691TrpAsn: 0.691 ± 0.029
0.281TrpPro: 0.281 ± 0.019
0.381TrpGln: 0.381 ± 0.021
0.464TrpArg: 0.464 ± 0.025
0.614TrpSer: 0.614 ± 0.025
0.542TrpThr: 0.542 ± 0.028
0.701TrpVal: 0.701 ± 0.034
0.159TrpTrp: 0.159 ± 0.013
0.454TrpTyr: 0.454 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.519TyrAla: 2.519 ± 0.06
0.321TyrCys: 0.321 ± 0.018
2.293TyrAsp: 2.293 ± 0.051
2.325TyrGlu: 2.325 ± 0.058
2.367TyrPhe: 2.367 ± 0.056
2.737TyrGly: 2.737 ± 0.053
0.829TyrHis: 0.829 ± 0.033
2.556TyrIle: 2.556 ± 0.05
2.644TyrLys: 2.644 ± 0.066
3.74TyrLeu: 3.74 ± 0.077
0.75TyrMet: 0.75 ± 0.028
2.507TyrAsn: 2.507 ± 0.063
1.514TyrPro: 1.514 ± 0.042
1.356TyrGln: 1.356 ± 0.035
1.892TyrArg: 1.892 ± 0.048
2.544TyrSer: 2.544 ± 0.054
2.483TyrThr: 2.483 ± 0.087
2.374TyrVal: 2.374 ± 0.046
0.462TyrTrp: 0.462 ± 0.021
1.811TyrTyr: 1.811 ± 0.05
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2826 proteins (970976 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski