Aspergillus sp. HF37
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8895 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3M2T9P4|A0A3M2T9P4_9EURO Uncharacterized protein OS=Aspergillus sp. HF37 OX=1960876 GN=PHISP_02649 PE=4 SV=1
MM1 pKa = 7.72 LLPYY5 pKa = 10.14 FGVVSIGQKK14 pKa = 9.28 QDD16 pKa = 2.86 NRR18 pKa = 11.84 SCDD21 pKa = 3.32 KK22 pKa = 11.03 QKK24 pKa = 11.36 NNWEE28 pKa = 3.98 IFLEE32 pKa = 5.18 GGDD35 pKa = 3.67 TDD37 pKa = 4.86 ANPPSVAAQYY47 pKa = 10.63 TNGDD51 pKa = 3.26 IFDD54 pKa = 4.19 IPFPLDD60 pKa = 4.52 DD61 pKa = 5.16 ILWDD65 pKa = 3.74 YY66 pKa = 10.95 QGPLMAHH73 pKa = 6.84 DD74 pKa = 4.89 HH75 pKa = 5.29 QQSALPADD83 pKa = 4.17 GQYY86 pKa = 10.85 HH87 pKa = 6.17 VDD89 pKa = 4.57 PEE91 pKa = 4.65 FGWSEE96 pKa = 3.71 IQLPFDD102 pKa = 3.25 WAVFSS107 pKa = 4.34
Molecular weight: 12.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.732
IPC2_protein 3.859
IPC_protein 3.834
Toseland 3.605
ProMoST 4.024
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.821
Rodwell 3.668
Grimsley 3.528
Solomon 3.834
Lehninger 3.795
Nozaki 3.973
DTASelect 4.253
Thurlkill 3.681
EMBOSS 3.834
Sillero 3.961
Patrickios 1.926
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.868
Protein with the highest isoelectric point:
>tr|A0A3M2SSD2|A0A3M2SSD2_9EURO Uncharacterized protein (Fragment) OS=Aspergillus sp. HF37 OX=1960876 GN=PHISP_08661 PE=4 SV=1
TT1 pKa = 7.1 LPSGRR6 pKa = 11.84 RR7 pKa = 11.84 FACASSRR14 pKa = 11.84 ARR16 pKa = 11.84 VASWRR21 pKa = 11.84 HH22 pKa = 4.71 RR23 pKa = 11.84 SRR25 pKa = 11.84 HH26 pKa = 3.62 GMRR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 PMTKK36 pKa = 8.79 RR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 PNRR42 pKa = 11.84 FTTRR46 pKa = 11.84 FPMLRR51 pKa = 11.84 SS52 pKa = 3.4
Molecular weight: 6.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.495
IPC2_protein 11.096
IPC_protein 12.72
Toseland 12.881
ProMoST 13.378
Dawson 12.881
Bjellqvist 12.881
Wikipedia 13.349
Rodwell 12.398
Grimsley 12.91
Solomon 13.378
Lehninger 13.276
Nozaki 12.881
DTASelect 12.881
Thurlkill 12.881
EMBOSS 13.378
Sillero 12.881
Patrickios 12.135
IPC_peptide 13.378
IPC2_peptide 12.369
IPC2.peptide.svr19 9.166
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8895
0
8895
3904314
50
5715
438.9
48.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.788 ± 0.022
1.162 ± 0.01
5.954 ± 0.018
6.282 ± 0.026
3.678 ± 0.015
7.143 ± 0.024
2.422 ± 0.012
4.392 ± 0.018
4.515 ± 0.022
8.747 ± 0.031
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.235 ± 0.01
3.584 ± 0.014
6.306 ± 0.031
4.063 ± 0.017
6.52 ± 0.022
8.364 ± 0.027
5.533 ± 0.015
6.263 ± 0.021
1.378 ± 0.01
2.671 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here