Amino acid dipepetide frequency for Aspergillus sp. HF37

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.447AlaAla: 9.447 ± 0.072	1.018AlaCys: 1.018 ± 0.019	4.824AlaAsp: 4.824 ± 0.038	5.458AlaGlu: 5.458 ± 0.05	3.134AlaPhe: 3.134 ± 0.031	6.415AlaGly: 6.415 ± 0.043	1.883AlaHis: 1.883 ± 0.023	3.828AlaIle: 3.828 ± 0.033	3.807AlaLys: 3.807 ± 0.039	7.633AlaLeu: 7.633 ± 0.056	2.027AlaMet: 2.027 ± 0.022	3.073AlaAsn: 3.073 ± 0.028	5.103AlaPro: 5.103 ± 0.055	3.517AlaGln: 3.517 ± 0.038	5.411AlaArg: 5.411 ± 0.038	7.48AlaSer: 7.48 ± 0.051	4.853AlaThr: 4.853 ± 0.038	5.729AlaVal: 5.729 ± 0.047	1.117AlaTrp: 1.117 ± 0.021	2.121AlaTyr: 2.121 ± 0.022	0.0AlaXaa: 0.0 ± 0.0
Cys	0.918CysAla: 0.918 ± 0.017	0.218CysCys: 0.218 ± 0.009	0.653CysAsp: 0.653 ± 0.013	0.588CysGlu: 0.588 ± 0.013	0.499CysPhe: 0.499 ± 0.012	0.898CysGly: 0.898 ± 0.019	0.309CysHis: 0.309 ± 0.009	0.611CysIle: 0.611 ± 0.014	0.424CysLys: 0.424 ± 0.012	1.206CysLeu: 1.206 ± 0.02	0.268CysMet: 0.268 ± 0.008	0.41CysAsn: 0.41 ± 0.011	0.645CysPro: 0.645 ± 0.016	0.434CysGln: 0.434 ± 0.011	0.767CysArg: 0.767 ± 0.016	0.867CysSer: 0.867 ± 0.016	0.61CysThr: 0.61 ± 0.014	0.77CysVal: 0.77 ± 0.015	0.185CysTrp: 0.185 ± 0.008	0.337CysTyr: 0.337 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	5.171AspAla: 5.171 ± 0.042	0.604AspCys: 0.604 ± 0.013	4.376AspAsp: 4.376 ± 0.048	4.69AspGlu: 4.69 ± 0.043	2.232AspPhe: 2.232 ± 0.025	4.341AspGly: 4.341 ± 0.043	1.304AspHis: 1.304 ± 0.021	2.922AspIle: 2.922 ± 0.029	2.271AspLys: 2.271 ± 0.024	5.146AspLeu: 5.146 ± 0.04	1.366AspMet: 1.366 ± 0.017	1.935AspAsn: 1.935 ± 0.022	3.7AspPro: 3.7 ± 0.032	2.068AspGln: 2.068 ± 0.025	3.484AspArg: 3.484 ± 0.035	4.46AspSer: 4.46 ± 0.038	3.022AspThr: 3.022 ± 0.029	3.924AspVal: 3.924 ± 0.033	0.875AspTrp: 0.875 ± 0.017	1.644AspTyr: 1.644 ± 0.021	0.0AspXaa: 0.0 ± 0.0
Glu	5.313GluAla: 5.313 ± 0.048	0.612GluCys: 0.612 ± 0.014	4.303GluAsp: 4.303 ± 0.041	5.426GluGlu: 5.426 ± 0.067	2.025GluPhe: 2.025 ± 0.024	3.97GluGly: 3.97 ± 0.035	1.456GluHis: 1.456 ± 0.021	2.762GluIle: 2.762 ± 0.026	3.536GluLys: 3.536 ± 0.038	5.16GluLeu: 5.16 ± 0.047	1.558GluMet: 1.558 ± 0.023	2.432GluAsn: 2.432 ± 0.027	3.118GluPro: 3.118 ± 0.042	2.618GluGln: 2.618 ± 0.035	4.211GluArg: 4.211 ± 0.041	4.763GluSer: 4.763 ± 0.043	3.46GluThr: 3.46 ± 0.032	3.573GluVal: 3.573 ± 0.034	0.859GluTrp: 0.859 ± 0.015	1.663GluTyr: 1.663 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	3.111PheAla: 3.111 ± 0.03	0.549PheCys: 0.549 ± 0.013	2.329PheAsp: 2.329 ± 0.022	2.113PheGlu: 2.113 ± 0.024	1.551PhePhe: 1.551 ± 0.021	2.926PheGly: 2.926 ± 0.036	0.933PheHis: 0.933 ± 0.017	1.616PheIle: 1.616 ± 0.025	1.364PheLys: 1.364 ± 0.02	3.473PheLeu: 3.473 ± 0.035	0.822PheMet: 0.822 ± 0.014	1.353PheAsn: 1.353 ± 0.021	2.009PhePro: 2.009 ± 0.026	1.386PheGln: 1.386 ± 0.019	2.083PheArg: 2.083 ± 0.025	2.973PheSer: 2.973 ± 0.032	1.999PheThr: 1.999 ± 0.024	2.48PheVal: 2.48 ± 0.028	0.613PheTrp: 0.613 ± 0.014	1.099PheTyr: 1.099 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	5.58GlyAla: 5.58 ± 0.043	0.881GlyCys: 0.881 ± 0.016	4.054GlyAsp: 4.054 ± 0.034	4.079GlyGlu: 4.079 ± 0.036	2.854GlyPhe: 2.854 ± 0.028	6.098GlyGly: 6.098 ± 0.067	1.778GlyHis: 1.778 ± 0.024	3.359GlyIle: 3.359 ± 0.033	3.445GlyLys: 3.445 ± 0.038	6.162GlyLeu: 6.162 ± 0.042	1.793GlyMet: 1.793 ± 0.024	2.542GlyAsn: 2.542 ± 0.026	3.654GlyPro: 3.654 ± 0.037	2.833GlyGln: 2.833 ± 0.032	4.594GlyArg: 4.594 ± 0.036	6.049GlySer: 6.049 ± 0.049	3.857GlyThr: 3.857 ± 0.032	4.586GlyVal: 4.586 ± 0.037	1.124GlyTrp: 1.124 ± 0.018	2.112GlyTyr: 2.112 ± 0.026	0.0GlyXaa: 0.0 ± 0.0
His	2.036HisAla: 2.036 ± 0.023	0.315HisCys: 0.315 ± 0.009	1.409HisAsp: 1.409 ± 0.022	1.409HisGlu: 1.409 ± 0.02	0.937HisPhe: 0.937 ± 0.016	1.844HisGly: 1.844 ± 0.024	0.831HisHis: 0.831 ± 0.017	1.108HisIle: 1.108 ± 0.019	0.809HisLys: 0.809 ± 0.013	2.246HisLeu: 2.246 ± 0.027	0.49HisMet: 0.49 ± 0.011	0.811HisAsn: 0.811 ± 0.016	1.78HisPro: 1.78 ± 0.022	0.956HisGln: 0.956 ± 0.015	1.679HisArg: 1.679 ± 0.023	1.864HisSer: 1.864 ± 0.025	1.173HisThr: 1.173 ± 0.018	1.508HisVal: 1.508 ± 0.019	0.337HisTrp: 0.337 ± 0.01	0.68HisTyr: 0.68 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	3.823IleAla: 3.823 ± 0.04	0.67IleCys: 0.67 ± 0.014	2.625IleAsp: 2.625 ± 0.027	2.584IleGlu: 2.584 ± 0.022	1.793IlePhe: 1.793 ± 0.024	2.985IleGly: 2.985 ± 0.036	1.152IleHis: 1.152 ± 0.017	1.995IleIle: 1.995 ± 0.027	1.791IleLys: 1.791 ± 0.026	4.1IleLeu: 4.1 ± 0.045	0.928IleMet: 0.928 ± 0.017	1.567IleAsn: 1.567 ± 0.022	2.881IlePro: 2.881 ± 0.028	1.756IleGln: 1.756 ± 0.022	2.689IleArg: 2.689 ± 0.029	3.38IleSer: 3.38 ± 0.035	2.363IleThr: 2.363 ± 0.026	2.924IleVal: 2.924 ± 0.031	0.631IleTrp: 0.631 ± 0.014	1.277IleTyr: 1.277 ± 0.02	0.0IleXaa: 0.0 ± 0.0
Lys	3.96LysAla: 3.96 ± 0.039	0.448LysCys: 0.448 ± 0.011	2.627LysAsp: 2.627 ± 0.031	3.155LysGlu: 3.155 ± 0.038	1.325LysPhe: 1.325 ± 0.017	2.955LysGly: 2.955 ± 0.031	1.097LysHis: 1.097 ± 0.017	1.91LysIle: 1.91 ± 0.027	2.964LysLys: 2.964 ± 0.052	3.745LysLeu: 3.745 ± 0.034	0.996LysMet: 0.996 ± 0.017	1.585LysAsn: 1.585 ± 0.022	2.834LysPro: 2.834 ± 0.029	1.836LysGln: 1.836 ± 0.023	3.423LysArg: 3.423 ± 0.04	3.356LysSer: 3.356 ± 0.03	2.526LysThr: 2.526 ± 0.029	2.587LysVal: 2.587 ± 0.033	0.589LysTrp: 0.589 ± 0.015	1.229LysTyr: 1.229 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	7.782LeuAla: 7.782 ± 0.051	1.138LeuCys: 1.138 ± 0.019	5.375LeuAsp: 5.375 ± 0.04	5.354LeuGlu: 5.354 ± 0.041	3.314LeuPhe: 3.314 ± 0.036	6.073LeuGly: 6.073 ± 0.044	2.246LeuHis: 2.246 ± 0.025	3.458LeuIle: 3.458 ± 0.039	3.806LeuLys: 3.806 ± 0.035	8.151LeuLeu: 8.151 ± 0.062	1.791LeuMet: 1.791 ± 0.022	3.06LeuAsn: 3.06 ± 0.028	5.331LeuPro: 5.331 ± 0.043	3.795LeuGln: 3.795 ± 0.037	6.063LeuArg: 6.063 ± 0.044	7.365LeuSer: 7.365 ± 0.047	4.388LeuThr: 4.388 ± 0.036	5.564LeuVal: 5.564 ± 0.046	1.112LeuTrp: 1.112 ± 0.017	2.305LeuTyr: 2.305 ± 0.026	0.0LeuXaa: 0.0 ± 0.0
Met	2.259MetAla: 2.259 ± 0.023	0.236MetCys: 0.236 ± 0.008	1.397MetAsp: 1.397 ± 0.019	1.368MetGlu: 1.368 ± 0.019	0.765MetPhe: 0.765 ± 0.016	1.596MetGly: 1.596 ± 0.024	0.516MetHis: 0.516 ± 0.011	0.934MetIle: 0.934 ± 0.017	1.012MetLys: 1.012 ± 0.016	1.858MetLeu: 1.858 ± 0.023	0.573MetMet: 0.573 ± 0.012	0.843MetAsn: 0.843 ± 0.015	1.357MetPro: 1.357 ± 0.018	0.918MetGln: 0.918 ± 0.017	1.367MetArg: 1.367 ± 0.019	1.899MetSer: 1.899 ± 0.022	1.266MetThr: 1.266 ± 0.019	1.425MetVal: 1.425 ± 0.02	0.25MetTrp: 0.25 ± 0.008	0.511MetTyr: 0.511 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	3.282AsnAla: 3.282 ± 0.031	0.417AsnCys: 0.417 ± 0.012	1.928AsnAsp: 1.928 ± 0.023	2.041AsnGlu: 2.041 ± 0.027	1.295AsnPhe: 1.295 ± 0.018	2.922AsnGly: 2.922 ± 0.036	0.851AsnHis: 0.851 ± 0.016	1.785AsnIle: 1.785 ± 0.019	1.418AsnLys: 1.418 ± 0.017	3.145AsnLeu: 3.145 ± 0.029	0.84AsnMet: 0.84 ± 0.015	1.34AsnAsn: 1.34 ± 0.022	2.59AsnPro: 2.59 ± 0.027	1.387AsnGln: 1.387 ± 0.022	2.07AsnArg: 2.07 ± 0.025	2.57AsnSer: 2.57 ± 0.029	2.066AsnThr: 2.066 ± 0.026	2.376AsnVal: 2.376 ± 0.026	0.519AsnTrp: 0.519 ± 0.011	0.998AsnTyr: 0.998 ± 0.017	0.0AsnXaa: 0.0 ± 0.0
Pro	5.909ProAla: 5.909 ± 0.043	0.491ProCys: 0.491 ± 0.012	3.639ProAsp: 3.639 ± 0.033	4.126ProGlu: 4.126 ± 0.038	2.134ProPhe: 2.134 ± 0.025	4.462ProGly: 4.462 ± 0.043	1.366ProHis: 1.366 ± 0.022	2.18ProIle: 2.18 ± 0.026	2.628ProLys: 2.628 ± 0.031	4.58ProLeu: 4.58 ± 0.036	1.15ProMet: 1.15 ± 0.02	2.231ProAsn: 2.231 ± 0.026	5.46ProPro: 5.46 ± 0.067	2.609ProGln: 2.609 ± 0.034	3.738ProArg: 3.738 ± 0.042	6.571ProSer: 6.571 ± 0.058	3.761ProThr: 3.761 ± 0.037	3.768ProVal: 3.768 ± 0.03	0.75ProTrp: 0.75 ± 0.013	1.506ProTyr: 1.506 ± 0.025	0.0ProXaa: 0.0 ± 0.0
Gln	3.442GlnAla: 3.442 ± 0.033	0.419GlnCys: 0.419 ± 0.012	2.116GlnAsp: 2.116 ± 0.023	2.465GlnGlu: 2.465 ± 0.03	1.345GlnPhe: 1.345 ± 0.02	2.576GlnGly: 2.576 ± 0.03	1.099GlnHis: 1.099 ± 0.017	1.714GlnIle: 1.714 ± 0.021	2.013GlnLys: 2.013 ± 0.024	3.445GlnLeu: 3.445 ± 0.034	0.928GlnMet: 0.928 ± 0.016	1.622GlnAsn: 1.622 ± 0.022	2.857GlnPro: 2.857 ± 0.039	2.337GlnGln: 2.337 ± 0.042	2.832GlnArg: 2.832 ± 0.03	3.307GlnSer: 3.307 ± 0.033	2.265GlnThr: 2.265 ± 0.028	2.231GlnVal: 2.231 ± 0.026	0.542GlnTrp: 0.542 ± 0.011	1.079GlnTyr: 1.079 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	5.097ArgAla: 5.097 ± 0.04	0.719ArgCys: 0.719 ± 0.016	3.784ArgAsp: 3.784 ± 0.042	4.248ArgGlu: 4.248 ± 0.043	2.329ArgPhe: 2.329 ± 0.027	4.134ArgGly: 4.134 ± 0.04	1.649ArgHis: 1.649 ± 0.024	2.866ArgIle: 2.866 ± 0.03	3.471ArgLys: 3.471 ± 0.035	5.75ArgLeu: 5.75 ± 0.044	1.462ArgMet: 1.462 ± 0.021	2.405ArgAsn: 2.405 ± 0.026	3.833ArgPro: 3.833 ± 0.041	2.771ArgGln: 2.771 ± 0.029	5.742ArgArg: 5.742 ± 0.058	5.191ArgSer: 5.191 ± 0.05	3.34ArgThr: 3.34 ± 0.032	3.769ArgVal: 3.769 ± 0.033	0.944ArgTrp: 0.944 ± 0.016	1.698ArgTyr: 1.698 ± 0.019	0.0ArgXaa: 0.0 ± 0.0
Ser	7.211SerAla: 7.211 ± 0.054	0.823SerCys: 0.823 ± 0.014	4.51SerAsp: 4.51 ± 0.042	4.411SerGlu: 4.411 ± 0.038	3.048SerPhe: 3.048 ± 0.034	5.914SerGly: 5.914 ± 0.046	2.016SerHis: 2.016 ± 0.023	3.518SerIle: 3.518 ± 0.032	3.642SerLys: 3.642 ± 0.034	7.294SerLeu: 7.294 ± 0.046	1.792SerMet: 1.792 ± 0.022	2.889SerAsn: 2.889 ± 0.03	5.798SerPro: 5.798 ± 0.051	3.443SerGln: 3.443 ± 0.036	5.487SerArg: 5.487 ± 0.048	8.808SerSer: 8.808 ± 0.074	5.153SerThr: 5.153 ± 0.044	4.836SerVal: 4.836 ± 0.034	1.069SerTrp: 1.069 ± 0.018	1.978SerTyr: 1.978 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	5.119ThrAla: 5.119 ± 0.036	0.639ThrCys: 0.639 ± 0.013	2.858ThrAsp: 2.858 ± 0.025	3.02ThrGlu: 3.02 ± 0.032	1.977ThrPhe: 1.977 ± 0.024	4.188ThrGly: 4.188 ± 0.036	1.2ThrHis: 1.2 ± 0.018	2.603ThrIle: 2.603 ± 0.029	2.333ThrLys: 2.333 ± 0.027	4.842ThrLeu: 4.842 ± 0.041	1.189ThrMet: 1.189 ± 0.016	1.82ThrAsn: 1.82 ± 0.023	4.225ThrPro: 4.225 ± 0.041	1.962ThrGln: 1.962 ± 0.023	3.089ThrArg: 3.089 ± 0.033	4.819ThrSer: 4.819 ± 0.039	3.634ThrThr: 3.634 ± 0.037	3.64ThrVal: 3.64 ± 0.032	0.747ThrTrp: 0.747 ± 0.014	1.425ThrTyr: 1.425 ± 0.019	0.0ThrXaa: 0.0 ± 0.0
Val	5.177ValAla: 5.177 ± 0.044	0.849ValCys: 0.849 ± 0.016	4.204ValAsp: 4.204 ± 0.036	3.917ValGlu: 3.917 ± 0.038	2.542ValPhe: 2.542 ± 0.029	4.198ValGly: 4.198 ± 0.039	1.486ValHis: 1.486 ± 0.019	2.753ValIle: 2.753 ± 0.032	2.718ValLys: 2.718 ± 0.027	5.732ValLeu: 5.732 ± 0.045	1.371ValMet: 1.371 ± 0.018	2.311ValAsn: 2.311 ± 0.023	3.801ValPro: 3.801 ± 0.029	2.431ValGln: 2.431 ± 0.025	3.806ValArg: 3.806 ± 0.031	4.932ValSer: 4.932 ± 0.038	3.314ValThr: 3.314 ± 0.032	4.446ValVal: 4.446 ± 0.047	0.873ValTrp: 0.873 ± 0.015	1.771ValTyr: 1.771 ± 0.024	0.0ValXaa: 0.0 ± 0.0
Trp	1.107TrpAla: 1.107 ± 0.018	0.177TrpCys: 0.177 ± 0.007	0.895TrpAsp: 0.895 ± 0.016	0.846TrpGlu: 0.846 ± 0.015	0.514TrpPhe: 0.514 ± 0.012	0.883TrpGly: 0.883 ± 0.017	0.336TrpHis: 0.336 ± 0.009	0.697TrpIle: 0.697 ± 0.013	0.722TrpLys: 0.722 ± 0.013	1.246TrpLeu: 1.246 ± 0.02	0.384TrpMet: 0.384 ± 0.009	0.587TrpAsn: 0.587 ± 0.014	0.577TrpPro: 0.577 ± 0.014	0.533TrpGln: 0.533 ± 0.012	0.973TrpArg: 0.973 ± 0.018	1.005TrpSer: 1.005 ± 0.017	0.816TrpThr: 0.816 ± 0.017	0.855TrpVal: 0.855 ± 0.012	0.252TrpTrp: 0.252 ± 0.007	0.375TrpTyr: 0.375 ± 0.011	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.132TyrAla: 2.132 ± 0.025	0.393TyrCys: 0.393 ± 0.011	1.632TyrAsp: 1.632 ± 0.023	1.523TyrGlu: 1.523 ± 0.022	1.168TyrPhe: 1.168 ± 0.019	2.053TyrGly: 2.053 ± 0.028	0.713TyrHis: 0.713 ± 0.014	1.294TyrIle: 1.294 ± 0.019	0.974TyrLys: 0.974 ± 0.018	2.554TyrLeu: 2.554 ± 0.03	0.622TyrMet: 0.622 ± 0.013	1.026TyrAsn: 1.026 ± 0.018	1.508TyrPro: 1.508 ± 0.02	1.043TyrGln: 1.043 ± 0.017	1.694TyrArg: 1.694 ± 0.023	1.982TyrSer: 1.982 ± 0.026	1.463TyrThr: 1.463 ± 0.023	1.641TyrVal: 1.641 ± 0.022	0.393TyrTrp: 0.393 ± 0.011	0.905TyrTyr: 0.905 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 8895 proteins (3904314 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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