Apolygus lucorum (Plant bug) (Lygocoris lucorum)
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 21887 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6A4J2M2|A0A6A4J2M2_APOLU Uncharacterized protein OS=Apolygus lucorum OX=248454 GN=GE061_10146 PE=4 SV=1
MM1 pKa = 7.4 SAGFFNFGLVSRR13 pKa = 11.84 GEE15 pKa = 4.41 KK16 pKa = 9.62 ILHH19 pKa = 5.07 QCNANKK25 pKa = 10.46 SVDD28 pKa = 3.35 ADD30 pKa = 3.35 EE31 pKa = 6.12 CYY33 pKa = 11.23 DD34 pKa = 3.7 HH35 pKa = 7.22 LLTNDD40 pKa = 4.44 EE41 pKa = 4.46 ISSAADD47 pKa = 3.4 NLCAALAEE55 pKa = 4.44 EE56 pKa = 5.15 GFLPEE61 pKa = 6.1 DD62 pKa = 4.65 DD63 pKa = 5.16 LPTIAGSDD71 pKa = 3.11 IFSNPGVLDD80 pKa = 4.18 AADD83 pKa = 4.21 DD84 pKa = 3.57 AHH86 pKa = 7.01 NNVMEE91 pKa = 5.38 PGDD94 pKa = 3.55 EE95 pKa = 4.04 HH96 pKa = 7.57 KK97 pKa = 11.05 INIVPDD103 pKa = 3.59 SSEE106 pKa = 4.04 TTEE109 pKa = 4.13 LRR111 pKa = 11.84 SSYY114 pKa = 9.86 EE115 pKa = 4.04 LEE117 pKa = 4.95 DD118 pKa = 5.41 ADD120 pKa = 3.93 MDD122 pKa = 4.27 QNDD125 pKa = 3.23 VEE127 pKa = 4.63 YY128 pKa = 10.79 VVEE131 pKa = 4.61 SEE133 pKa = 4.44 QEE135 pKa = 3.94 LEE137 pKa = 4.01 ATQLEE142 pKa = 4.98 VCANN146 pKa = 3.59
Molecular weight: 15.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.726
IPC2_protein 3.668
IPC_protein 3.656
Toseland 3.452
ProMoST 3.808
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.554
Rodwell 3.49
Grimsley 3.363
Solomon 3.63
Lehninger 3.579
Nozaki 3.757
DTASelect 3.948
Thurlkill 3.503
EMBOSS 3.567
Sillero 3.77
Patrickios 0.985
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.722
Protein with the highest isoelectric point:
>tr|A0A6A4KCV8|A0A6A4KCV8_APOLU Uncharacterized protein OS=Apolygus lucorum OX=248454 GN=GE061_11958 PE=3 SV=1
CC1 pKa = 7.02 AAWGIARR8 pKa = 11.84 GIAWGIAWGIARR20 pKa = 11.84 GIARR24 pKa = 11.84 GIARR28 pKa = 11.84 GIAWGIARR36 pKa = 11.84 GIARR40 pKa = 11.84 GIARR44 pKa = 11.84 GIARR48 pKa = 11.84 GIARR52 pKa = 11.84 GIARR56 pKa = 11.84 GIARR60 pKa = 11.84 GIARR64 pKa = 11.84 GIAWGIAWGIARR76 pKa = 11.84 GIAWGIARR84 pKa = 11.84 GIAWGIAWGIARR96 pKa = 11.84 GIARR100 pKa = 11.84 GIARR104 pKa = 11.84 GIARR108 pKa = 11.84 GIARR112 pKa = 11.84 GIARR116 pKa = 11.84 GIARR120 pKa = 11.84 GIARR124 pKa = 11.84 GIARR128 pKa = 11.84 GIARR132 pKa = 11.84 GIARR136 pKa = 11.84 GIAWGIARR144 pKa = 11.84 GIARR148 pKa = 11.84 GIGGG152 pKa = 3.66
Molecular weight: 15.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.316
IPC_protein 12.939
Toseland 13.1
ProMoST 13.598
Dawson 13.1
Bjellqvist 13.1
Wikipedia 13.583
Rodwell 12.603
Grimsley 13.144
Solomon 13.598
Lehninger 13.495
Nozaki 13.1
DTASelect 13.1
Thurlkill 13.1
EMBOSS 13.598
Sillero 13.1
Patrickios 12.31
IPC_peptide 13.598
IPC2_peptide 12.588
IPC2.peptide.svr19 9.269
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
21887
0
21887
9312152
35
8678
425.5
47.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.222 ± 0.019
1.929 ± 0.012
5.515 ± 0.017
6.811 ± 0.025
3.742 ± 0.013
5.892 ± 0.026
2.397 ± 0.008
5.152 ± 0.014
6.514 ± 0.025
8.896 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.352 ± 0.008
4.447 ± 0.014
5.811 ± 0.027
3.956 ± 0.014
5.565 ± 0.016
8.404 ± 0.025
5.796 ± 0.019
6.482 ± 0.017
1.169 ± 0.006
2.947 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here