Mycobacterium phage Phantastic

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A023W6B2|A0A023W6B2_9CAUD Uncharacterized protein OS=Mycobacterium phage Phantastic OX=1486426 GN=63 PE=4 SV=1
MM1 pKa = 7.61TLPKK5 pKa = 10.51NLTSHH10 pKa = 6.86SDD12 pKa = 3.79DD13 pKa = 3.97IDD15 pKa = 3.13WDD17 pKa = 3.85AVHH20 pKa = 7.43DD21 pKa = 4.1YY22 pKa = 10.92VYY24 pKa = 10.98EE25 pKa = 5.18GDD27 pKa = 4.83DD28 pKa = 4.06DD29 pKa = 4.96NEE31 pKa = 4.05

Molecular weight: 3.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A023W662|A0A023W662_9CAUD Tail protein OS=Mycobacterium phage Phantastic OX=1486426 GN=2 PE=4 SV=1
MM1 pKa = 7.93RR2 pKa = 11.84IFVLGEE8 pKa = 3.56RR9 pKa = 11.84RR10 pKa = 11.84IEE12 pKa = 4.19SHH14 pKa = 6.15RR15 pKa = 11.84HH16 pKa = 4.83DD17 pKa = 4.7PRR19 pKa = 11.84NEE21 pKa = 3.89QYY23 pKa = 11.29GVTSGEE29 pKa = 3.83RR30 pKa = 11.84HH31 pKa = 4.83VGPRR35 pKa = 11.84EE36 pKa = 3.82RR37 pKa = 11.84SRR39 pKa = 11.84VDD41 pKa = 2.72HH42 pKa = 6.79HH43 pKa = 7.68GSDD46 pKa = 3.41TRR48 pKa = 11.84NATEE52 pKa = 3.54GSMPFRR58 pKa = 11.84SAPLFLPTDD67 pKa = 4.1LTSHH71 pKa = 6.94KK72 pKa = 10.45GMVMNKK78 pKa = 6.67QQRR81 pKa = 11.84VYY83 pKa = 11.2LDD85 pKa = 3.19GKK87 pKa = 10.61VYY89 pKa = 10.18ICRR92 pKa = 11.84GTNIVRR98 pKa = 11.84VRR100 pKa = 3.33

Molecular weight: 11.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

91

0

91

15791

25

1011

173.5

19.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.556 ± 0.421

0.823 ± 0.124

6.333 ± 0.264

6.979 ± 0.333

3.306 ± 0.196

8.353 ± 0.551

2.172 ± 0.189

4.756 ± 0.171

4.256 ± 0.234

8.055 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.482 ± 0.153

3.375 ± 0.174

5.725 ± 0.356

3.888 ± 0.404

6.922 ± 0.335

5.117 ± 0.214

5.497 ± 0.216

7.454 ± 0.227

1.893 ± 0.158

3.059 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski