Amino acid dipepetide frequency for Mycobacterium phage Phantastic

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.703AlaAla: 10.703 ± 1.195
0.697AlaCys: 0.697 ± 0.202
5.51AlaAsp: 5.51 ± 0.68
6.966AlaGlu: 6.966 ± 0.661
2.723AlaPhe: 2.723 ± 0.517
7.6AlaGly: 7.6 ± 0.835
1.963AlaHis: 1.963 ± 0.354
4.307AlaIle: 4.307 ± 0.621
4.877AlaLys: 4.877 ± 0.6
8.676AlaLeu: 8.676 ± 0.713
2.723AlaMet: 2.723 ± 0.433
2.533AlaAsn: 2.533 ± 0.4
5.383AlaPro: 5.383 ± 0.851
3.8AlaGln: 3.8 ± 0.46
5.763AlaArg: 5.763 ± 0.518
4.37AlaSer: 4.37 ± 0.481
5.193AlaThr: 5.193 ± 0.557
7.663AlaVal: 7.663 ± 0.684
1.9AlaTrp: 1.9 ± 0.325
2.217AlaTyr: 2.217 ± 0.44
0.0AlaXaa: 0.0 ± 0.0
Cys
0.507CysAla: 0.507 ± 0.177
0.127CysCys: 0.127 ± 0.132
0.443CysAsp: 0.443 ± 0.169
0.823CysGlu: 0.823 ± 0.196
0.127CysPhe: 0.127 ± 0.082
0.76CysGly: 0.76 ± 0.226
0.253CysHis: 0.253 ± 0.124
0.317CysIle: 0.317 ± 0.128
0.38CysLys: 0.38 ± 0.147
0.57CysLeu: 0.57 ± 0.246
0.127CysMet: 0.127 ± 0.084
0.507CysAsn: 0.507 ± 0.158
0.507CysPro: 0.507 ± 0.203
0.507CysGln: 0.507 ± 0.168
0.823CysArg: 0.823 ± 0.217
0.253CysSer: 0.253 ± 0.141
0.317CysThr: 0.317 ± 0.187
0.38CysVal: 0.38 ± 0.147
0.063CysTrp: 0.063 ± 0.067
0.443CysTyr: 0.443 ± 0.191
0.0CysXaa: 0.0 ± 0.0
Asp
6.27AspAla: 6.27 ± 0.666
0.633AspCys: 0.633 ± 0.199
4.307AspAsp: 4.307 ± 0.739
4.37AspGlu: 4.37 ± 0.506
2.533AspPhe: 2.533 ± 0.369
5.066AspGly: 5.066 ± 0.474
1.52AspHis: 1.52 ± 0.366
3.103AspIle: 3.103 ± 0.488
1.647AspLys: 1.647 ± 0.315
5.256AspLeu: 5.256 ± 0.599
1.013AspMet: 1.013 ± 0.252
2.343AspAsn: 2.343 ± 0.349
4.623AspPro: 4.623 ± 0.707
2.28AspGln: 2.28 ± 0.449
3.737AspArg: 3.737 ± 0.604
3.357AspSer: 3.357 ± 0.505
3.673AspThr: 3.673 ± 0.444
3.737AspVal: 3.737 ± 0.397
1.647AspTrp: 1.647 ± 0.293
2.153AspTyr: 2.153 ± 0.43
0.0AspXaa: 0.0 ± 0.0
Glu
7.03GluAla: 7.03 ± 0.66
0.57GluCys: 0.57 ± 0.193
4.623GluAsp: 4.623 ± 0.538
4.433GluGlu: 4.433 ± 0.556
2.66GluPhe: 2.66 ± 0.359
5.32GluGly: 5.32 ± 0.75
1.33GluHis: 1.33 ± 0.308
2.787GluIle: 2.787 ± 0.517
2.343GluLys: 2.343 ± 0.345
6.586GluLeu: 6.586 ± 0.688
1.773GluMet: 1.773 ± 0.374
2.217GluAsn: 2.217 ± 0.407
3.23GluPro: 3.23 ± 0.506
2.913GluGln: 2.913 ± 0.439
5.256GluArg: 5.256 ± 0.677
3.673GluSer: 3.673 ± 0.463
3.61GluThr: 3.61 ± 0.554
5.51GluVal: 5.51 ± 0.647
1.013GluTrp: 1.013 ± 0.239
2.913GluTyr: 2.913 ± 0.519
0.0GluXaa: 0.0 ± 0.0
Phe
3.737PheAla: 3.737 ± 0.5
0.253PheCys: 0.253 ± 0.149
3.167PheAsp: 3.167 ± 0.481
2.533PheGlu: 2.533 ± 0.412
0.507PhePhe: 0.507 ± 0.178
3.167PheGly: 3.167 ± 0.583
0.57PheHis: 0.57 ± 0.207
1.203PheIle: 1.203 ± 0.288
1.013PheLys: 1.013 ± 0.265
2.913PheLeu: 2.913 ± 0.558
0.507PheMet: 0.507 ± 0.217
1.71PheAsn: 1.71 ± 0.345
1.71PhePro: 1.71 ± 0.383
1.077PheGln: 1.077 ± 0.232
1.773PheArg: 1.773 ± 0.38
1.647PheSer: 1.647 ± 0.31
1.837PheThr: 1.837 ± 0.304
2.407PheVal: 2.407 ± 0.4
0.443PheTrp: 0.443 ± 0.149
0.887PheTyr: 0.887 ± 0.202
0.0PheXaa: 0.0 ± 0.0
Gly
6.016GlyAla: 6.016 ± 0.883
0.887GlyCys: 0.887 ± 0.257
5.383GlyAsp: 5.383 ± 0.684
4.877GlyGlu: 4.877 ± 0.651
3.483GlyPhe: 3.483 ± 0.471
7.283GlyGly: 7.283 ± 1.106
1.773GlyHis: 1.773 ± 0.341
3.737GlyIle: 3.737 ± 0.41
3.99GlyLys: 3.99 ± 0.496
6.713GlyLeu: 6.713 ± 0.796
2.09GlyMet: 2.09 ± 0.317
3.23GlyAsn: 3.23 ± 0.605
6.27GlyPro: 6.27 ± 2.869
3.293GlyGln: 3.293 ± 0.571
4.687GlyArg: 4.687 ± 0.568
4.75GlySer: 4.75 ± 0.689
5.256GlyThr: 5.256 ± 0.569
5.636GlyVal: 5.636 ± 0.542
1.457GlyTrp: 1.457 ± 0.264
2.723GlyTyr: 2.723 ± 0.419
0.0GlyXaa: 0.0 ± 0.0
His
1.963HisAla: 1.963 ± 0.327
0.127HisCys: 0.127 ± 0.091
1.583HisAsp: 1.583 ± 0.318
1.267HisGlu: 1.267 ± 0.333
0.697HisPhe: 0.697 ± 0.21
2.407HisGly: 2.407 ± 0.534
0.57HisHis: 0.57 ± 0.145
1.52HisIle: 1.52 ± 0.296
0.76HisLys: 0.76 ± 0.198
1.203HisLeu: 1.203 ± 0.349
0.38HisMet: 0.38 ± 0.161
0.57HisAsn: 0.57 ± 0.199
1.33HisPro: 1.33 ± 0.272
0.95HisGln: 0.95 ± 0.283
2.217HisArg: 2.217 ± 0.418
0.57HisSer: 0.57 ± 0.206
1.14HisThr: 1.14 ± 0.28
1.14HisVal: 1.14 ± 0.278
0.38HisTrp: 0.38 ± 0.179
0.95HisTyr: 0.95 ± 0.252
0.0HisXaa: 0.0 ± 0.0
Ile
5.066IleAla: 5.066 ± 0.619
0.443IleCys: 0.443 ± 0.149
3.42IleAsp: 3.42 ± 0.461
4.117IleGlu: 4.117 ± 0.51
1.583IlePhe: 1.583 ± 0.322
4.117IleGly: 4.117 ± 0.395
1.203IleHis: 1.203 ± 0.264
1.647IleIle: 1.647 ± 0.368
1.393IleLys: 1.393 ± 0.281
2.47IleLeu: 2.47 ± 0.388
0.697IleMet: 0.697 ± 0.283
1.773IleAsn: 1.773 ± 0.284
3.8IlePro: 3.8 ± 0.424
1.9IleGln: 1.9 ± 0.604
3.61IleArg: 3.61 ± 0.368
2.09IleSer: 2.09 ± 0.478
2.977IleThr: 2.977 ± 0.4
3.04IleVal: 3.04 ± 0.377
0.887IleTrp: 0.887 ± 0.235
1.33IleTyr: 1.33 ± 0.249
0.0IleXaa: 0.0 ± 0.0
Lys
4.813LysAla: 4.813 ± 0.631
0.253LysCys: 0.253 ± 0.126
2.343LysAsp: 2.343 ± 0.362
2.723LysGlu: 2.723 ± 0.411
0.95LysPhe: 0.95 ± 0.251
3.8LysGly: 3.8 ± 0.584
0.887LysHis: 0.887 ± 0.237
1.9LysIle: 1.9 ± 0.412
2.723LysLys: 2.723 ± 0.525
3.23LysLeu: 3.23 ± 0.414
0.95LysMet: 0.95 ± 0.279
1.33LysAsn: 1.33 ± 0.261
2.407LysPro: 2.407 ± 0.521
1.457LysGln: 1.457 ± 0.25
2.787LysArg: 2.787 ± 0.566
2.217LysSer: 2.217 ± 0.37
2.407LysThr: 2.407 ± 0.508
3.61LysVal: 3.61 ± 0.52
0.76LysTrp: 0.76 ± 0.218
1.013LysTyr: 1.013 ± 0.256
0.0LysXaa: 0.0 ± 0.0
Leu
7.6LeuAla: 7.6 ± 0.755
0.38LeuCys: 0.38 ± 0.155
5.383LeuAsp: 5.383 ± 0.552
6.396LeuGlu: 6.396 ± 0.699
2.28LeuPhe: 2.28 ± 0.381
6.523LeuGly: 6.523 ± 0.791
1.457LeuHis: 1.457 ± 0.36
4.37LeuIle: 4.37 ± 0.547
3.483LeuLys: 3.483 ± 0.451
5.89LeuLeu: 5.89 ± 0.627
2.343LeuMet: 2.343 ± 0.399
2.723LeuAsn: 2.723 ± 0.407
4.243LeuPro: 4.243 ± 0.459
2.66LeuGln: 2.66 ± 0.553
6.776LeuArg: 6.776 ± 0.73
4.433LeuSer: 4.433 ± 0.557
4.75LeuThr: 4.75 ± 0.516
5.193LeuVal: 5.193 ± 0.577
1.14LeuTrp: 1.14 ± 0.285
2.533LeuTyr: 2.533 ± 0.459
0.0LeuXaa: 0.0 ± 0.0
Met
3.167MetAla: 3.167 ± 0.46
0.0MetCys: 0.0 ± 0.0
1.71MetAsp: 1.71 ± 0.322
1.52MetGlu: 1.52 ± 0.262
0.76MetPhe: 0.76 ± 0.21
1.52MetGly: 1.52 ± 0.366
0.253MetHis: 0.253 ± 0.162
1.583MetIle: 1.583 ± 0.32
1.457MetLys: 1.457 ± 0.302
1.203MetLeu: 1.203 ± 0.277
0.57MetMet: 0.57 ± 0.185
1.203MetAsn: 1.203 ± 0.271
1.52MetPro: 1.52 ± 0.287
0.57MetGln: 0.57 ± 0.223
1.647MetArg: 1.647 ± 0.437
1.71MetSer: 1.71 ± 0.285
1.963MetThr: 1.963 ± 0.336
1.647MetVal: 1.647 ± 0.316
0.19MetTrp: 0.19 ± 0.12
0.633MetTyr: 0.633 ± 0.232
0.0MetXaa: 0.0 ± 0.0
Asn
3.547AsnAla: 3.547 ± 0.506
0.443AsnCys: 0.443 ± 0.143
1.9AsnAsp: 1.9 ± 0.335
1.837AsnGlu: 1.837 ± 0.304
1.267AsnPhe: 1.267 ± 0.323
3.293AsnGly: 3.293 ± 0.464
1.077AsnHis: 1.077 ± 0.249
2.153AsnIle: 2.153 ± 0.383
1.013AsnLys: 1.013 ± 0.249
2.09AsnLeu: 2.09 ± 0.327
0.887AsnMet: 0.887 ± 0.208
0.823AsnAsn: 0.823 ± 0.202
2.217AsnPro: 2.217 ± 0.37
1.14AsnGln: 1.14 ± 0.269
2.027AsnArg: 2.027 ± 0.477
1.52AsnSer: 1.52 ± 0.306
1.647AsnThr: 1.647 ± 0.258
2.85AsnVal: 2.85 ± 0.408
0.76AsnTrp: 0.76 ± 0.194
1.267AsnTyr: 1.267 ± 0.253
0.0AsnXaa: 0.0 ± 0.0
Pro
5.193ProAla: 5.193 ± 0.557
0.443ProCys: 0.443 ± 0.195
3.673ProAsp: 3.673 ± 0.464
3.927ProGlu: 3.927 ± 0.588
1.647ProPhe: 1.647 ± 0.326
5.066ProGly: 5.066 ± 0.801
1.393ProHis: 1.393 ± 0.269
2.533ProIle: 2.533 ± 0.338
2.723ProLys: 2.723 ± 0.527
4.307ProLeu: 4.307 ± 0.704
1.013ProMet: 1.013 ± 0.287
2.47ProAsn: 2.47 ± 0.477
2.723ProPro: 2.723 ± 0.536
3.737ProGln: 3.737 ± 1.994
3.103ProArg: 3.103 ± 0.49
2.66ProSer: 2.66 ± 0.345
3.547ProThr: 3.547 ± 0.556
4.623ProVal: 4.623 ± 0.565
0.95ProTrp: 0.95 ± 0.333
1.52ProTyr: 1.52 ± 0.401
0.0ProXaa: 0.0 ± 0.0
Gln
3.8GlnAla: 3.8 ± 0.688
0.127GlnCys: 0.127 ± 0.086
1.457GlnAsp: 1.457 ± 0.262
2.28GlnGlu: 2.28 ± 0.357
1.33GlnPhe: 1.33 ± 0.33
4.75GlnGly: 4.75 ± 2.677
0.887GlnHis: 0.887 ± 0.224
1.773GlnIle: 1.773 ± 0.334
1.33GlnLys: 1.33 ± 0.291
4.18GlnLeu: 4.18 ± 0.733
1.077GlnMet: 1.077 ± 0.304
0.633GlnAsn: 0.633 ± 0.228
1.963GlnPro: 1.963 ± 0.32
2.47GlnGln: 2.47 ± 0.531
2.723GlnArg: 2.723 ± 0.47
2.217GlnSer: 2.217 ± 0.403
1.583GlnThr: 1.583 ± 0.446
2.533GlnVal: 2.533 ± 0.435
0.76GlnTrp: 0.76 ± 0.198
1.013GlnTyr: 1.013 ± 0.225
0.0GlnXaa: 0.0 ± 0.0
Arg
5.763ArgAla: 5.763 ± 0.638
0.76ArgCys: 0.76 ± 0.274
3.673ArgAsp: 3.673 ± 0.502
5.193ArgGlu: 5.193 ± 0.641
2.217ArgPhe: 2.217 ± 0.36
4.56ArgGly: 4.56 ± 0.623
1.583ArgHis: 1.583 ± 0.319
3.8ArgIle: 3.8 ± 0.443
3.8ArgLys: 3.8 ± 0.588
5.193ArgLeu: 5.193 ± 0.545
2.47ArgMet: 2.47 ± 0.439
2.533ArgAsn: 2.533 ± 0.415
3.04ArgPro: 3.04 ± 0.494
2.027ArgGln: 2.027 ± 0.385
5.953ArgArg: 5.953 ± 0.765
3.99ArgSer: 3.99 ± 0.498
3.167ArgThr: 3.167 ± 0.489
5.636ArgVal: 5.636 ± 0.553
1.837ArgTrp: 1.837 ± 0.447
2.027ArgTyr: 2.027 ± 0.362
0.0ArgXaa: 0.0 ± 0.0
Ser
3.927SerAla: 3.927 ± 0.479
0.127SerCys: 0.127 ± 0.081
2.09SerAsp: 2.09 ± 0.421
4.117SerGlu: 4.117 ± 0.439
2.66SerPhe: 2.66 ± 0.436
5.51SerGly: 5.51 ± 0.717
1.393SerHis: 1.393 ± 0.315
2.407SerIle: 2.407 ± 0.344
2.28SerLys: 2.28 ± 0.47
4.117SerLeu: 4.117 ± 0.586
1.52SerMet: 1.52 ± 0.289
1.457SerAsn: 1.457 ± 0.342
2.217SerPro: 2.217 ± 0.326
1.837SerGln: 1.837 ± 0.477
3.8SerArg: 3.8 ± 0.512
2.47SerSer: 2.47 ± 0.529
2.217SerThr: 2.217 ± 0.375
4.243SerVal: 4.243 ± 0.43
1.267SerTrp: 1.267 ± 0.282
1.52SerTyr: 1.52 ± 0.288
0.0SerXaa: 0.0 ± 0.0
Thr
4.813ThrAla: 4.813 ± 0.464
0.823ThrCys: 0.823 ± 0.241
3.23ThrAsp: 3.23 ± 0.649
3.673ThrGlu: 3.673 ± 0.5
1.963ThrPhe: 1.963 ± 0.356
4.307ThrGly: 4.307 ± 0.618
0.887ThrHis: 0.887 ± 0.274
2.28ThrIle: 2.28 ± 0.468
2.09ThrLys: 2.09 ± 0.439
5.953ThrLeu: 5.953 ± 0.725
1.14ThrMet: 1.14 ± 0.204
1.52ThrAsn: 1.52 ± 0.391
3.863ThrPro: 3.863 ± 0.548
2.28ThrGln: 2.28 ± 0.424
3.8ThrArg: 3.8 ± 0.569
2.47ThrSer: 2.47 ± 0.444
2.723ThrThr: 2.723 ± 0.478
4.56ThrVal: 4.56 ± 0.523
1.077ThrTrp: 1.077 ± 0.287
1.52ThrTyr: 1.52 ± 0.274
0.0ThrXaa: 0.0 ± 0.0
Val
7.093ValAla: 7.093 ± 0.786
0.507ValCys: 0.507 ± 0.159
5.763ValAsp: 5.763 ± 0.759
5.51ValGlu: 5.51 ± 0.684
2.343ValPhe: 2.343 ± 0.482
5.193ValGly: 5.193 ± 0.714
1.71ValHis: 1.71 ± 0.381
3.293ValIle: 3.293 ± 0.461
3.547ValLys: 3.547 ± 0.559
5.573ValLeu: 5.573 ± 0.604
1.52ValMet: 1.52 ± 0.29
2.407ValAsn: 2.407 ± 0.405
4.053ValPro: 4.053 ± 0.567
2.09ValGln: 2.09 ± 0.289
5.13ValArg: 5.13 ± 0.718
4.623ValSer: 4.623 ± 0.545
4.18ValThr: 4.18 ± 0.502
6.396ValVal: 6.396 ± 0.516
1.013ValTrp: 1.013 ± 0.287
2.597ValTyr: 2.597 ± 0.429
0.0ValXaa: 0.0 ± 0.0
Trp
1.71TrpAla: 1.71 ± 0.391
0.317TrpCys: 0.317 ± 0.183
1.393TrpAsp: 1.393 ± 0.329
1.203TrpGlu: 1.203 ± 0.267
0.633TrpPhe: 0.633 ± 0.182
1.393TrpGly: 1.393 ± 0.308
0.38TrpHis: 0.38 ± 0.151
1.583TrpIle: 1.583 ± 0.354
0.697TrpLys: 0.697 ± 0.194
1.33TrpLeu: 1.33 ± 0.261
0.823TrpMet: 0.823 ± 0.178
0.57TrpAsn: 0.57 ± 0.18
0.697TrpPro: 0.697 ± 0.206
0.76TrpGln: 0.76 ± 0.216
0.887TrpArg: 0.887 ± 0.249
0.95TrpSer: 0.95 ± 0.283
1.14TrpThr: 1.14 ± 0.298
1.33TrpVal: 1.33 ± 0.297
0.697TrpTrp: 0.697 ± 0.192
0.443TrpTyr: 0.443 ± 0.163
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.85TyrAla: 2.85 ± 0.447
0.317TyrCys: 0.317 ± 0.14
2.28TyrAsp: 2.28 ± 0.515
2.027TyrGlu: 2.027 ± 0.379
0.697TyrPhe: 0.697 ± 0.224
1.9TyrGly: 1.9 ± 0.354
0.633TyrHis: 0.633 ± 0.208
1.267TyrIle: 1.267 ± 0.294
1.013TyrLys: 1.013 ± 0.294
3.103TyrLeu: 3.103 ± 0.386
1.14TyrMet: 1.14 ± 0.27
1.203TyrAsn: 1.203 ± 0.282
1.457TyrPro: 1.457 ± 0.261
1.14TyrGln: 1.14 ± 0.271
2.723TyrArg: 2.723 ± 0.467
1.203TyrSer: 1.203 ± 0.225
1.647TyrThr: 1.647 ± 0.311
2.407TyrVal: 2.407 ± 0.387
0.697TyrTrp: 0.697 ± 0.213
0.887TyrTyr: 0.887 ± 0.318
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 91 proteins (15791 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski