Salmonella phage PMBT28

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2L0V196|A0A2L0V196_9CAUD Uncharacterized protein OS=Salmonella phage PMBT28 OX=2081904 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 10.53GIYY5 pKa = 9.25QVVGSDD11 pKa = 3.69GVVLTAIAAEE21 pKa = 4.28TEE23 pKa = 4.24EE24 pKa = 4.13QARR27 pKa = 11.84AAMAEE32 pKa = 4.15FEE34 pKa = 4.49AADD37 pKa = 4.01LLYY40 pKa = 11.47VNDD43 pKa = 3.73TTLDD47 pKa = 3.34HH48 pKa = 6.96GEE50 pKa = 4.15LYY52 pKa = 10.8EE53 pKa = 4.92LGNDD57 pKa = 3.51EE58 pKa = 4.6VGSLIIIAVADD69 pKa = 3.78EE70 pKa = 4.4AEE72 pKa = 4.2ARR74 pKa = 11.84ATADD78 pKa = 3.01GMGEE82 pKa = 4.16LFEE85 pKa = 3.93EE86 pKa = 5.41HH87 pKa = 6.49YY88 pKa = 10.63DD89 pKa = 3.37IRR91 pKa = 11.84HH92 pKa = 4.96VATFF96 pKa = 3.66

Molecular weight: 10.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2L0V193|A0A2L0V193_9CAUD Uncharacterized protein OS=Salmonella phage PMBT28 OX=2081904 PE=4 SV=1
MM1 pKa = 7.02HH2 pKa = 7.25QLMNLIASVTTMEE15 pKa = 4.17VAFGFVLAVLLALFLVPMYY34 pKa = 10.35ILHH37 pKa = 5.44CTGRR41 pKa = 11.84NLLANNVGRR50 pKa = 11.84VAHH53 pKa = 6.33RR54 pKa = 11.84EE55 pKa = 3.76WMFGSTVIIYY65 pKa = 9.46SRR67 pKa = 11.84WPISKK72 pKa = 9.33GWKK75 pKa = 8.54GCSARR80 pKa = 11.84KK81 pKa = 7.86KK82 pKa = 8.46TSRR85 pKa = 11.84SCMLTMGTVQIEE97 pKa = 4.52LIRR100 pKa = 11.84LCRR103 pKa = 11.84SQAPIYY109 pKa = 10.09RR110 pKa = 11.84ATYY113 pKa = 9.24KK114 pKa = 9.77QAKK117 pKa = 8.23KK118 pKa = 9.87HH119 pKa = 5.1AA120 pKa = 4.45

Molecular weight: 13.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

14927

39

1066

219.5

24.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.707 ± 0.35

0.965 ± 0.12

6.224 ± 0.2

6.378 ± 0.308

3.41 ± 0.207

7.898 ± 0.17

1.916 ± 0.181

5.232 ± 0.188

5.272 ± 0.295

8.24 ± 0.234

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.028 ± 0.127

4.241 ± 0.225

4.341 ± 0.255

3.738 ± 0.149

5.721 ± 0.285

5.205 ± 0.269

6.465 ± 0.276

6.706 ± 0.297

1.869 ± 0.186

3.443 ± 0.235
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski