Amino acid dipepetide frequency for Salmonella phage PMBT28

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.385AlaAla: 10.385 ± 1.226
1.005AlaCys: 1.005 ± 0.253
6.432AlaAsp: 6.432 ± 0.448
6.633AlaGlu: 6.633 ± 0.663
2.814AlaPhe: 2.814 ± 0.496
6.834AlaGly: 6.834 ± 0.699
1.407AlaHis: 1.407 ± 0.294
4.489AlaIle: 4.489 ± 0.537
6.432AlaLys: 6.432 ± 0.811
8.375AlaLeu: 8.375 ± 0.998
4.087AlaMet: 4.087 ± 0.668
4.02AlaAsn: 4.02 ± 0.699
3.015AlaPro: 3.015 ± 0.44
3.484AlaGln: 3.484 ± 0.461
4.422AlaArg: 4.422 ± 0.595
4.824AlaSer: 4.824 ± 0.478
7.102AlaThr: 7.102 ± 0.767
6.365AlaVal: 6.365 ± 0.709
1.474AlaTrp: 1.474 ± 0.396
3.484AlaTyr: 3.484 ± 0.448
0.0AlaXaa: 0.0 ± 0.0
Cys
0.67CysAla: 0.67 ± 0.211
0.134CysCys: 0.134 ± 0.1
0.938CysAsp: 0.938 ± 0.274
0.871CysGlu: 0.871 ± 0.227
0.201CysPhe: 0.201 ± 0.106
0.67CysGly: 0.67 ± 0.23
0.268CysHis: 0.268 ± 0.128
0.335CysIle: 0.335 ± 0.15
0.469CysLys: 0.469 ± 0.2
0.469CysLeu: 0.469 ± 0.201
0.402CysMet: 0.402 ± 0.175
0.335CysAsn: 0.335 ± 0.13
0.536CysPro: 0.536 ± 0.158
0.469CysGln: 0.469 ± 0.18
1.072CysArg: 1.072 ± 0.262
0.335CysSer: 0.335 ± 0.14
0.536CysThr: 0.536 ± 0.175
0.469CysVal: 0.469 ± 0.181
0.201CysTrp: 0.201 ± 0.116
0.268CysTyr: 0.268 ± 0.118
0.0CysXaa: 0.0 ± 0.0
Asp
5.695AspAla: 5.695 ± 0.784
0.804AspCys: 0.804 ± 0.27
3.953AspAsp: 3.953 ± 0.503
3.618AspGlu: 3.618 ± 0.552
2.68AspPhe: 2.68 ± 0.374
5.963AspGly: 5.963 ± 0.658
1.273AspHis: 1.273 ± 0.219
3.551AspIle: 3.551 ± 0.477
3.149AspLys: 3.149 ± 0.39
6.097AspLeu: 6.097 ± 0.693
1.742AspMet: 1.742 ± 0.305
1.876AspAsn: 1.876 ± 0.476
3.015AspPro: 3.015 ± 0.428
2.01AspGln: 2.01 ± 0.313
3.35AspArg: 3.35 ± 0.561
2.546AspSer: 2.546 ± 0.47
3.819AspThr: 3.819 ± 0.432
3.886AspVal: 3.886 ± 0.529
1.206AspTrp: 1.206 ± 0.3
2.01AspTyr: 2.01 ± 0.392
0.0AspXaa: 0.0 ± 0.0
Glu
6.767GluAla: 6.767 ± 0.778
0.603GluCys: 0.603 ± 0.23
3.216GluAsp: 3.216 ± 0.467
4.556GluGlu: 4.556 ± 0.654
2.546GluPhe: 2.546 ± 0.456
3.216GluGly: 3.216 ± 0.409
1.876GluHis: 1.876 ± 0.405
3.082GluIle: 3.082 ± 0.383
3.015GluLys: 3.015 ± 0.504
6.499GluLeu: 6.499 ± 0.518
1.675GluMet: 1.675 ± 0.32
2.546GluAsn: 2.546 ± 0.367
2.68GluPro: 2.68 ± 0.407
3.283GluGln: 3.283 ± 0.464
3.819GluArg: 3.819 ± 0.641
3.015GluSer: 3.015 ± 0.412
2.814GluThr: 2.814 ± 0.37
4.958GluVal: 4.958 ± 0.575
1.139GluTrp: 1.139 ± 0.268
2.479GluTyr: 2.479 ± 0.421
0.0GluXaa: 0.0 ± 0.0
Phe
2.278PheAla: 2.278 ± 0.427
0.335PheCys: 0.335 ± 0.154
2.412PheAsp: 2.412 ± 0.387
2.613PheGlu: 2.613 ± 0.491
0.804PhePhe: 0.804 ± 0.246
2.881PheGly: 2.881 ± 0.483
0.469PheHis: 0.469 ± 0.166
2.345PheIle: 2.345 ± 0.395
2.01PheLys: 2.01 ± 0.312
2.345PheLeu: 2.345 ± 0.326
1.005PheMet: 1.005 ± 0.284
1.608PheAsn: 1.608 ± 0.332
1.809PhePro: 1.809 ± 0.251
0.804PheGln: 0.804 ± 0.308
1.809PheArg: 1.809 ± 0.371
1.876PheSer: 1.876 ± 0.309
2.546PheThr: 2.546 ± 0.358
2.747PheVal: 2.747 ± 0.487
0.536PheTrp: 0.536 ± 0.146
0.871PheTyr: 0.871 ± 0.193
0.0PheXaa: 0.0 ± 0.0
Gly
6.499GlyAla: 6.499 ± 0.747
0.804GlyCys: 0.804 ± 0.276
4.824GlyAsp: 4.824 ± 0.516
4.422GlyGlu: 4.422 ± 0.416
2.479GlyPhe: 2.479 ± 0.354
6.901GlyGly: 6.901 ± 0.637
1.876GlyHis: 1.876 ± 0.316
3.685GlyIle: 3.685 ± 0.533
4.221GlyLys: 4.221 ± 0.524
6.633GlyLeu: 6.633 ± 0.572
2.412GlyMet: 2.412 ± 0.408
3.149GlyAsn: 3.149 ± 0.448
3.015GlyPro: 3.015 ± 0.534
3.886GlyGln: 3.886 ± 0.555
3.551GlyArg: 3.551 ± 0.441
5.829GlySer: 5.829 ± 0.702
6.231GlyThr: 6.231 ± 0.785
4.02GlyVal: 4.02 ± 0.55
1.474GlyTrp: 1.474 ± 0.274
3.082GlyTyr: 3.082 ± 0.478
0.0GlyXaa: 0.0 ± 0.0
His
1.474HisAla: 1.474 ± 0.327
0.402HisCys: 0.402 ± 0.146
0.737HisAsp: 0.737 ± 0.234
1.206HisGlu: 1.206 ± 0.29
0.737HisPhe: 0.737 ± 0.254
2.01HisGly: 2.01 ± 0.404
0.268HisHis: 0.268 ± 0.127
0.737HisIle: 0.737 ± 0.214
0.871HisLys: 0.871 ± 0.209
1.407HisLeu: 1.407 ± 0.354
0.603HisMet: 0.603 ± 0.227
0.536HisAsn: 0.536 ± 0.159
1.005HisPro: 1.005 ± 0.234
1.005HisGln: 1.005 ± 0.219
1.206HisArg: 1.206 ± 0.292
0.938HisSer: 0.938 ± 0.253
1.072HisThr: 1.072 ± 0.366
1.474HisVal: 1.474 ± 0.32
0.603HisTrp: 0.603 ± 0.174
0.871HisTyr: 0.871 ± 0.236
0.0HisXaa: 0.0 ± 0.0
Ile
4.69IleAla: 4.69 ± 0.406
0.402IleCys: 0.402 ± 0.14
4.422IleAsp: 4.422 ± 0.522
4.623IleGlu: 4.623 ± 0.487
1.675IlePhe: 1.675 ± 0.311
3.417IleGly: 3.417 ± 0.454
1.407IleHis: 1.407 ± 0.311
2.077IleIle: 2.077 ± 0.427
2.546IleLys: 2.546 ± 0.404
3.149IleLeu: 3.149 ± 0.537
0.536IleMet: 0.536 ± 0.165
3.484IleAsn: 3.484 ± 0.361
2.345IlePro: 2.345 ± 0.394
1.608IleGln: 1.608 ± 0.378
3.953IleArg: 3.953 ± 0.558
2.077IleSer: 2.077 ± 0.294
3.819IleThr: 3.819 ± 0.818
3.551IleVal: 3.551 ± 0.498
0.603IleTrp: 0.603 ± 0.189
1.943IleTyr: 1.943 ± 0.304
0.0IleXaa: 0.0 ± 0.0
Lys
5.561LysAla: 5.561 ± 0.798
0.402LysCys: 0.402 ± 0.162
2.546LysAsp: 2.546 ± 0.464
4.221LysGlu: 4.221 ± 0.62
1.541LysPhe: 1.541 ± 0.286
4.891LysGly: 4.891 ± 0.615
0.67LysHis: 0.67 ± 0.202
2.747LysIle: 2.747 ± 0.406
3.551LysLys: 3.551 ± 0.468
4.221LysLeu: 4.221 ± 0.555
1.876LysMet: 1.876 ± 0.352
2.01LysAsn: 2.01 ± 0.343
2.613LysPro: 2.613 ± 0.542
1.742LysGln: 1.742 ± 0.351
2.546LysArg: 2.546 ± 0.373
2.278LysSer: 2.278 ± 0.292
3.082LysThr: 3.082 ± 0.461
3.618LysVal: 3.618 ± 0.408
0.804LysTrp: 0.804 ± 0.245
1.809LysTyr: 1.809 ± 0.323
0.0LysXaa: 0.0 ± 0.0
Leu
8.844LeuAla: 8.844 ± 0.841
0.938LeuCys: 0.938 ± 0.212
6.231LeuAsp: 6.231 ± 0.748
4.891LeuGlu: 4.891 ± 0.684
2.881LeuPhe: 2.881 ± 0.382
5.695LeuGly: 5.695 ± 0.565
0.871LeuHis: 0.871 ± 0.216
4.288LeuIle: 4.288 ± 0.638
4.288LeuLys: 4.288 ± 0.519
6.298LeuLeu: 6.298 ± 0.665
3.015LeuMet: 3.015 ± 0.453
4.288LeuAsn: 4.288 ± 0.571
3.551LeuPro: 3.551 ± 0.517
2.412LeuGln: 2.412 ± 0.42
5.025LeuArg: 5.025 ± 0.656
4.757LeuSer: 4.757 ± 0.687
5.293LeuThr: 5.293 ± 0.556
5.36LeuVal: 5.36 ± 0.57
1.273LeuTrp: 1.273 ± 0.347
2.211LeuTyr: 2.211 ± 0.398
0.0LeuXaa: 0.0 ± 0.0
Met
3.618MetAla: 3.618 ± 0.546
0.201MetCys: 0.201 ± 0.114
1.474MetAsp: 1.474 ± 0.379
2.278MetGlu: 2.278 ± 0.363
1.139MetPhe: 1.139 ± 0.2
1.474MetGly: 1.474 ± 0.303
0.804MetHis: 0.804 ± 0.215
1.206MetIle: 1.206 ± 0.268
1.876MetLys: 1.876 ± 0.363
2.278MetLeu: 2.278 ± 0.347
0.469MetMet: 0.469 ± 0.139
1.474MetAsn: 1.474 ± 0.3
0.737MetPro: 0.737 ± 0.215
1.273MetGln: 1.273 ± 0.315
2.144MetArg: 2.144 ± 0.35
1.943MetSer: 1.943 ± 0.339
2.278MetThr: 2.278 ± 0.396
1.742MetVal: 1.742 ± 0.355
0.469MetTrp: 0.469 ± 0.188
1.407MetTyr: 1.407 ± 0.333
0.0MetXaa: 0.0 ± 0.0
Asn
3.484AsnAla: 3.484 ± 0.581
0.335AsnCys: 0.335 ± 0.149
2.613AsnAsp: 2.613 ± 0.422
2.814AsnGlu: 2.814 ± 0.427
1.072AsnPhe: 1.072 ± 0.214
3.685AsnGly: 3.685 ± 0.553
0.804AsnHis: 0.804 ± 0.238
2.345AsnIle: 2.345 ± 0.356
2.211AsnLys: 2.211 ± 0.349
3.015AsnLeu: 3.015 ± 0.468
1.072AsnMet: 1.072 ± 0.288
2.077AsnAsn: 2.077 ± 0.461
2.479AsnPro: 2.479 ± 0.406
1.675AsnGln: 1.675 ± 0.278
2.144AsnArg: 2.144 ± 0.27
2.345AsnSer: 2.345 ± 0.422
2.881AsnThr: 2.881 ± 0.526
2.68AsnVal: 2.68 ± 0.666
1.206AsnTrp: 1.206 ± 0.272
1.474AsnTyr: 1.474 ± 0.36
0.0AsnXaa: 0.0 ± 0.0
Pro
4.087ProAla: 4.087 ± 0.448
0.268ProCys: 0.268 ± 0.158
3.417ProAsp: 3.417 ± 0.527
3.082ProGlu: 3.082 ± 0.515
1.943ProPhe: 1.943 ± 0.412
3.484ProGly: 3.484 ± 0.514
0.469ProHis: 0.469 ± 0.177
2.144ProIle: 2.144 ± 0.377
1.809ProLys: 1.809 ± 0.407
2.412ProLeu: 2.412 ± 0.395
1.541ProMet: 1.541 ± 0.337
2.479ProAsn: 2.479 ± 0.488
2.01ProPro: 2.01 ± 0.387
1.072ProGln: 1.072 ± 0.237
2.278ProArg: 2.278 ± 0.362
2.278ProSer: 2.278 ± 0.368
3.484ProThr: 3.484 ± 0.589
3.417ProVal: 3.417 ± 0.598
0.536ProTrp: 0.536 ± 0.216
1.206ProTyr: 1.206 ± 0.258
0.0ProXaa: 0.0 ± 0.0
Gln
3.283GlnAla: 3.283 ± 0.531
0.402GlnCys: 0.402 ± 0.154
2.01GlnAsp: 2.01 ± 0.338
1.742GlnGlu: 1.742 ± 0.312
1.407GlnPhe: 1.407 ± 0.338
2.68GlnGly: 2.68 ± 0.388
1.072GlnHis: 1.072 ± 0.251
2.814GlnIle: 2.814 ± 0.386
1.742GlnLys: 1.742 ± 0.286
2.613GlnLeu: 2.613 ± 0.522
1.206GlnMet: 1.206 ± 0.277
1.675GlnAsn: 1.675 ± 0.331
2.211GlnPro: 2.211 ± 0.399
1.541GlnGln: 1.541 ± 0.354
2.412GlnArg: 2.412 ± 0.471
2.144GlnSer: 2.144 ± 0.397
1.943GlnThr: 1.943 ± 0.31
2.278GlnVal: 2.278 ± 0.372
1.072GlnTrp: 1.072 ± 0.33
1.139GlnTyr: 1.139 ± 0.266
0.0GlnXaa: 0.0 ± 0.0
Arg
6.231ArgAla: 6.231 ± 0.75
0.335ArgCys: 0.335 ± 0.154
3.618ArgAsp: 3.618 ± 0.434
2.881ArgGlu: 2.881 ± 0.467
2.479ArgPhe: 2.479 ± 0.349
3.819ArgGly: 3.819 ± 0.506
1.541ArgHis: 1.541 ± 0.278
3.283ArgIle: 3.283 ± 0.494
3.283ArgLys: 3.283 ± 0.494
4.891ArgLeu: 4.891 ± 0.574
1.541ArgMet: 1.541 ± 0.312
2.211ArgAsn: 2.211 ± 0.365
1.742ArgPro: 1.742 ± 0.316
1.943ArgGln: 1.943 ± 0.335
3.685ArgArg: 3.685 ± 0.517
3.417ArgSer: 3.417 ± 0.536
2.881ArgThr: 2.881 ± 0.496
4.288ArgVal: 4.288 ± 0.615
1.474ArgTrp: 1.474 ± 0.321
1.675ArgTyr: 1.675 ± 0.361
0.0ArgXaa: 0.0 ± 0.0
Ser
4.02SerAla: 4.02 ± 0.506
0.67SerCys: 0.67 ± 0.192
3.216SerAsp: 3.216 ± 0.525
2.881SerGlu: 2.881 ± 0.374
1.809SerPhe: 1.809 ± 0.416
5.628SerGly: 5.628 ± 0.726
1.072SerHis: 1.072 ± 0.248
2.814SerIle: 2.814 ± 0.552
2.278SerLys: 2.278 ± 0.491
4.355SerLeu: 4.355 ± 0.542
1.809SerMet: 1.809 ± 0.361
2.211SerAsn: 2.211 ± 0.368
2.345SerPro: 2.345 ± 0.45
1.675SerGln: 1.675 ± 0.252
3.417SerArg: 3.417 ± 0.359
2.345SerSer: 2.345 ± 0.39
2.881SerThr: 2.881 ± 0.469
3.551SerVal: 3.551 ± 0.417
0.67SerTrp: 0.67 ± 0.282
2.345SerTyr: 2.345 ± 0.342
0.0SerXaa: 0.0 ± 0.0
Thr
6.566ThrAla: 6.566 ± 0.702
0.469ThrCys: 0.469 ± 0.201
3.618ThrAsp: 3.618 ± 0.434
3.685ThrGlu: 3.685 ± 0.403
2.278ThrPhe: 2.278 ± 0.285
5.695ThrGly: 5.695 ± 0.556
0.938ThrHis: 0.938 ± 0.258
4.355ThrIle: 4.355 ± 0.503
2.881ThrLys: 2.881 ± 0.47
5.293ThrLeu: 5.293 ± 0.932
1.876ThrMet: 1.876 ± 0.331
1.34ThrAsn: 1.34 ± 0.322
3.551ThrPro: 3.551 ± 0.608
2.211ThrGln: 2.211 ± 0.492
3.953ThrArg: 3.953 ± 0.48
3.216ThrSer: 3.216 ± 0.633
4.288ThrThr: 4.288 ± 0.527
4.623ThrVal: 4.623 ± 0.597
1.273ThrTrp: 1.273 ± 0.285
2.546ThrTyr: 2.546 ± 0.397
0.0ThrXaa: 0.0 ± 0.0
Val
8.375ValAla: 8.375 ± 0.723
0.603ValCys: 0.603 ± 0.214
4.154ValAsp: 4.154 ± 0.454
3.953ValGlu: 3.953 ± 0.57
2.278ValPhe: 2.278 ± 0.447
4.623ValGly: 4.623 ± 0.66
1.206ValHis: 1.206 ± 0.317
3.819ValIle: 3.819 ± 0.546
3.216ValLys: 3.216 ± 0.425
5.829ValLeu: 5.829 ± 0.558
2.01ValMet: 2.01 ± 0.387
2.814ValAsn: 2.814 ± 0.427
3.015ValPro: 3.015 ± 0.567
2.948ValGln: 2.948 ± 0.464
3.618ValArg: 3.618 ± 0.565
2.948ValSer: 2.948 ± 0.513
3.886ValThr: 3.886 ± 0.404
4.623ValVal: 4.623 ± 0.748
1.407ValTrp: 1.407 ± 0.254
1.742ValTyr: 1.742 ± 0.285
0.0ValXaa: 0.0 ± 0.0
Trp
2.211TrpAla: 2.211 ± 0.321
0.067TrpCys: 0.067 ± 0.074
0.804TrpAsp: 0.804 ± 0.214
1.139TrpGlu: 1.139 ± 0.228
0.536TrpPhe: 0.536 ± 0.22
1.34TrpGly: 1.34 ± 0.418
0.402TrpHis: 0.402 ± 0.162
0.469TrpIle: 0.469 ± 0.148
0.737TrpLys: 0.737 ± 0.195
2.211TrpLeu: 2.211 ± 0.495
0.469TrpMet: 0.469 ± 0.203
0.938TrpAsn: 0.938 ± 0.276
0.67TrpPro: 0.67 ± 0.271
1.072TrpGln: 1.072 ± 0.245
1.005TrpArg: 1.005 ± 0.251
1.206TrpSer: 1.206 ± 0.237
1.005TrpThr: 1.005 ± 0.279
0.938TrpVal: 0.938 ± 0.259
0.469TrpTrp: 0.469 ± 0.193
1.005TrpTyr: 1.005 ± 0.276
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.345TyrAla: 2.345 ± 0.464
0.469TyrCys: 0.469 ± 0.165
1.608TyrAsp: 1.608 ± 0.33
1.675TyrGlu: 1.675 ± 0.239
0.804TyrPhe: 0.804 ± 0.203
4.087TyrGly: 4.087 ± 0.566
0.469TyrHis: 0.469 ± 0.157
1.742TyrIle: 1.742 ± 0.352
2.144TyrLys: 2.144 ± 0.306
4.02TyrLeu: 4.02 ± 0.518
0.871TyrMet: 0.871 ± 0.245
1.34TyrAsn: 1.34 ± 0.233
1.072TyrPro: 1.072 ± 0.35
1.273TyrGln: 1.273 ± 0.332
1.809TyrArg: 1.809 ± 0.385
1.742TyrSer: 1.742 ± 0.404
2.814TyrThr: 2.814 ± 0.509
2.479TyrVal: 2.479 ± 0.393
0.804TyrTrp: 0.804 ± 0.233
0.871TyrTyr: 0.871 ± 0.266
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (14927 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski