Parasulfuritortus cantonensis
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2975 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V2NW73|A0A4V2NW73_9PROT Histidine kinase OS=Parasulfuritortus cantonensis OX=2528202 GN=EZJ19_05430 PE=4 SV=1
MM1 pKa = 7.2 GTGLLNISLSGLNAAMAGIRR21 pKa = 11.84 TVQQNIANANTAGYY35 pKa = 7.37 TRR37 pKa = 11.84 QEE39 pKa = 4.44 VVQSASTPQYY49 pKa = 10.89 SGGGYY54 pKa = 10.14 LGTGVQVDD62 pKa = 4.18 TVRR65 pKa = 11.84 RR66 pKa = 11.84 IYY68 pKa = 10.83 DD69 pKa = 3.23 QFLTSQTQNYY79 pKa = 6.08 QTQLSAAEE87 pKa = 4.76 AYY89 pKa = 10.12 SSYY92 pKa = 11.05 ASEE95 pKa = 3.93 VDD97 pKa = 3.27 ALLGGSSTGLSTPLQTFFSAVSEE120 pKa = 4.39 VANDD124 pKa = 3.78 PTSLTARR131 pKa = 11.84 QQMISTANSLASRR144 pKa = 11.84 FNLLADD150 pKa = 3.62 NLQSIKK156 pKa = 10.17 TEE158 pKa = 3.89 INSEE162 pKa = 4.0 VSTIATQINSYY173 pKa = 10.9 ADD175 pKa = 3.43 QIQSLNAEE183 pKa = 4.14 IARR186 pKa = 11.84 AQGSGQTPNDD196 pKa = 3.89 LLDD199 pKa = 4.03 QRR201 pKa = 11.84 DD202 pKa = 3.66 QLVNEE207 pKa = 4.61 LGKK210 pKa = 10.54 LVNVSSIQQSDD221 pKa = 3.6 GTVAVMIGNGQPLVVGTAVRR241 pKa = 11.84 HH242 pKa = 4.46 VVAVSDD248 pKa = 4.31 SADD251 pKa = 3.4 PTQTILAMEE260 pKa = 4.93 IPNSTATEE268 pKa = 4.25 VIDD271 pKa = 4.25 PSSITSGSLGGLFAFRR287 pKa = 11.84 DD288 pKa = 3.84 EE289 pKa = 4.34 VLNPSIADD297 pKa = 4.02 LDD299 pKa = 3.78 QLAQSLADD307 pKa = 3.69 AFNSQHH313 pKa = 5.77 EE314 pKa = 4.26 AGYY317 pKa = 10.46 DD318 pKa = 3.48 LNGAAGTAFFSYY330 pKa = 10.41 SATSPAGTLAVVISQASQVAAASQGVSAAADD361 pKa = 3.52 AGNSGDD367 pKa = 3.65 ASIGAVTLSEE377 pKa = 4.37 TVTRR381 pKa = 11.84 PLADD385 pKa = 3.19 APYY388 pKa = 8.54 TLSYY392 pKa = 10.74 NAGTVTVTDD401 pKa = 4.1 ASANVVGSFTYY412 pKa = 10.32 TSGATMTFDD421 pKa = 4.97 GISVAITDD429 pKa = 4.07 GSGGIADD436 pKa = 4.57 GDD438 pKa = 4.0 TFTIDD443 pKa = 3.05 NSGAYY448 pKa = 9.57 SGPGDD453 pKa = 3.77 NGNALLLAGLQNEE466 pKa = 4.29 AVIGSSTLSEE476 pKa = 4.46 YY477 pKa = 9.63 NTALVGRR484 pKa = 11.84 NATYY488 pKa = 11.12 ANSADD493 pKa = 3.75 TNVSNFTSLYY503 pKa = 7.31 QQSYY507 pKa = 11.39 DD508 pKa = 3.44 SLQSVSGVNLDD519 pKa = 3.63 EE520 pKa = 4.59 EE521 pKa = 4.55 AVKK524 pKa = 10.7 LIQLQQAYY532 pKa = 8.59 QAAAKK537 pKa = 9.6 AIQVSSTLFDD547 pKa = 3.61 AVLGAMQQ554 pKa = 4.42
Molecular weight: 56.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.783
IPC_protein 3.808
Toseland 3.579
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.732
Rodwell 3.63
Grimsley 3.478
Solomon 3.795
Lehninger 3.745
Nozaki 3.91
DTASelect 4.164
Thurlkill 3.63
EMBOSS 3.745
Sillero 3.923
Patrickios 0.973
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.815
Protein with the highest isoelectric point:
>tr|A0A4R1B8X1|A0A4R1B8X1_9PROT tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase OS=Parasulfuritortus cantonensis OX=2528202 GN=miaB PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 9.97 QPSVVRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.57 RR14 pKa = 11.84 THH16 pKa = 5.82 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MKK23 pKa = 8.8 TKK25 pKa = 10.36 GGRR28 pKa = 11.84 AVINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.58 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2975
0
2975
945077
26
2409
317.7
34.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.877 ± 0.079
0.979 ± 0.019
5.814 ± 0.042
5.712 ± 0.044
3.417 ± 0.027
8.463 ± 0.043
2.308 ± 0.023
4.268 ± 0.035
3.233 ± 0.042
11.139 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.397 ± 0.021
2.603 ± 0.029
5.143 ± 0.034
3.333 ± 0.026
7.364 ± 0.051
4.719 ± 0.034
4.677 ± 0.052
7.538 ± 0.038
1.419 ± 0.02
2.595 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here