Human respirovirus 3
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|T1UFE7|T1UFE7_9MONO Nucleocapsid OS=Human respirovirus 3 OX=11216 GN=N PE=3 SV=1
MM1 pKa = 7.66 LSLFDD6 pKa = 3.51 TFNARR11 pKa = 11.84 RR12 pKa = 11.84 QEE14 pKa = 4.42 NITKK18 pKa = 10.13 SAGGAIIPGQKK29 pKa = 8.49 NTVSIFALGPTITDD43 pKa = 3.78 DD44 pKa = 3.84 NEE46 pKa = 4.12 KK47 pKa = 8.4 MTLALLFLSHH57 pKa = 6.91 SLDD60 pKa = 3.36 NEE62 pKa = 4.0 KK63 pKa = 10.13 QHH65 pKa = 5.9 AQRR68 pKa = 11.84 AGFLVSLLSMAYY80 pKa = 10.11 ANPEE84 pKa = 4.11 LYY86 pKa = 9.54 LTTNGSNADD95 pKa = 3.65 VKK97 pKa = 10.62 YY98 pKa = 10.03 VIYY101 pKa = 9.75 MIEE104 pKa = 4.4 KK105 pKa = 9.19 DD106 pKa = 4.42 LKK108 pKa = 7.46 RR109 pKa = 11.84 QKK111 pKa = 10.92 YY112 pKa = 9.39 GGFVVKK118 pKa = 9.19 TRR120 pKa = 11.84 EE121 pKa = 3.85 MIYY124 pKa = 10.74 EE125 pKa = 4.24 KK126 pKa = 8.71 TTDD129 pKa = 3.53 WIFGSDD135 pKa = 3.59 LDD137 pKa = 4.24 YY138 pKa = 11.4 DD139 pKa = 4.09 QEE141 pKa = 4.43 TMLQNGRR148 pKa = 11.84 NNSTIEE154 pKa = 4.1 DD155 pKa = 3.8 LVHH158 pKa = 5.73 TFGYY162 pKa = 9.16 PSCLGAIIIQIWIVLVKK179 pKa = 10.55 AITSISGLRR188 pKa = 11.84 KK189 pKa = 9.95 GFFTRR194 pKa = 11.84 LEE196 pKa = 4.02 AFRR199 pKa = 11.84 QDD201 pKa = 3.09 GTVQAGLVLSGDD213 pKa = 3.79 TVDD216 pKa = 4.06 QIGSIMRR223 pKa = 11.84 SQQSLVTLMVEE234 pKa = 4.35 TLITMNTSRR243 pKa = 11.84 NDD245 pKa = 2.98 LTTIEE250 pKa = 4.61 KK251 pKa = 10.41 NIQIVGNYY259 pKa = 8.94 IRR261 pKa = 11.84 DD262 pKa = 3.47 AGLASFFNTIRR273 pKa = 11.84 YY274 pKa = 9.22 GIEE277 pKa = 3.27 TRR279 pKa = 11.84 MAALTLSTLRR289 pKa = 11.84 PDD291 pKa = 3.44 INRR294 pKa = 11.84 LKK296 pKa = 11.05 ALMEE300 pKa = 4.81 LYY302 pKa = 10.37 LSKK305 pKa = 10.93 GPRR308 pKa = 11.84 APFICILRR316 pKa = 11.84 DD317 pKa = 4.01 PIHH320 pKa = 7.17 GEE322 pKa = 4.01 FAPGNYY328 pKa = 8.42 PAIWSYY334 pKa = 11.86 AMGVAVVQNRR344 pKa = 11.84 AMQQYY349 pKa = 7.78 VTGRR353 pKa = 11.84 SYY355 pKa = 11.82 LDD357 pKa = 2.77 IDD359 pKa = 3.9 MFQLGQAVARR369 pKa = 11.84 DD370 pKa = 4.05 AEE372 pKa = 4.34 AQMSSTLEE380 pKa = 4.14 DD381 pKa = 3.65 EE382 pKa = 5.37 LGVTHH387 pKa = 6.45 EE388 pKa = 4.81 AKK390 pKa = 10.53 EE391 pKa = 4.05 SLKK394 pKa = 10.74 RR395 pKa = 11.84 HH396 pKa = 4.97 IRR398 pKa = 11.84 NINSSDD404 pKa = 3.39 TSFHH408 pKa = 6.63 KK409 pKa = 9.67 PTGGSAIEE417 pKa = 3.91 MAIDD421 pKa = 3.52 EE422 pKa = 4.56 EE423 pKa = 4.66 PEE425 pKa = 4.04 QFEE428 pKa = 4.06 NRR430 pKa = 11.84 ADD432 pKa = 3.69 QEE434 pKa = 4.25 QDD436 pKa = 2.96 GGPQSSIIQYY446 pKa = 10.37 AWAEE450 pKa = 4.29 GNRR453 pKa = 11.84 SDD455 pKa = 5.87 DD456 pKa = 3.59 RR457 pKa = 11.84 TEE459 pKa = 3.74 QATEE463 pKa = 3.58 SDD465 pKa = 4.0 NIKK468 pKa = 9.5 TEE470 pKa = 3.81 QQNIRR475 pKa = 11.84 DD476 pKa = 4.04 RR477 pKa = 11.84 LNKK480 pKa = 9.99 RR481 pKa = 11.84 LNDD484 pKa = 3.55 KK485 pKa = 10.24 KK486 pKa = 10.89 KK487 pKa = 10.46 QGSQPPTNPTNRR499 pKa = 11.84 TNQDD503 pKa = 3.38 EE504 pKa = 4.24 IDD506 pKa = 3.84 DD507 pKa = 4.33 LFNAFGSNN515 pKa = 3.14
Molecular weight: 57.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.154
IPC2_protein 5.181
IPC_protein 5.13
Toseland 5.08
ProMoST 5.283
Dawson 5.13
Bjellqvist 5.258
Wikipedia 5.016
Rodwell 5.041
Grimsley 5.003
Solomon 5.13
Lehninger 5.092
Nozaki 5.258
DTASelect 5.436
Thurlkill 5.092
EMBOSS 5.067
Sillero 5.334
Patrickios 4.279
IPC_peptide 5.143
IPC2_peptide 5.321
IPC2.peptide.svr19 5.367
Protein with the highest isoelectric point:
>tr|T1UFE7|T1UFE7_9MONO Nucleocapsid OS=Human respirovirus 3 OX=11216 GN=N PE=3 SV=1
MM1 pKa = 7.77 SITNSAIYY9 pKa = 8.25 TFPEE13 pKa = 4.1 SSFSEE18 pKa = 4.16 NGHH21 pKa = 6.56 IEE23 pKa = 4.0 PLPLKK28 pKa = 10.87 VNEE31 pKa = 3.85 QRR33 pKa = 11.84 KK34 pKa = 8.51 AVPHH38 pKa = 5.81 IRR40 pKa = 11.84 VAKK43 pKa = 9.94 IGNPPKK49 pKa = 10.43 HH50 pKa = 6.24 GSRR53 pKa = 11.84 YY54 pKa = 10.04 LDD56 pKa = 3.28 VFLLGFFEE64 pKa = 4.38 MEE66 pKa = 4.46 RR67 pKa = 11.84 IKK69 pKa = 11.03 DD70 pKa = 3.49 KK71 pKa = 11.28 YY72 pKa = 11.22 GSVNDD77 pKa = 4.42 LDD79 pKa = 4.65 SDD81 pKa = 4.03 PSYY84 pKa = 10.74 KK85 pKa = 10.39 VCGSGSLPIGLAKK98 pKa = 9.84 YY99 pKa = 8.27 TGNDD103 pKa = 3.24 QEE105 pKa = 4.84 LLQAATKK112 pKa = 10.69 LDD114 pKa = 3.32 IEE116 pKa = 4.43 VRR118 pKa = 11.84 RR119 pKa = 11.84 TVKK122 pKa = 10.28 AKK124 pKa = 10.32 EE125 pKa = 3.87 MVVYY129 pKa = 8.58 TVQNIKK135 pKa = 10.55 PEE137 pKa = 4.47 LYY139 pKa = 8.73 PWSSRR144 pKa = 11.84 LRR146 pKa = 11.84 KK147 pKa = 10.12 GMLFDD152 pKa = 4.35 ANKK155 pKa = 10.02 VALAPQCLPLDD166 pKa = 3.63 RR167 pKa = 11.84 SIKK170 pKa = 9.94 FRR172 pKa = 11.84 VIFVNCTAIGSITLFKK188 pKa = 10.26 IPKK191 pKa = 9.75 SMASLSLPNTISINLQVHH209 pKa = 6.54 IKK211 pKa = 9.74 TGVQTDD217 pKa = 3.52 SKK219 pKa = 11.19 GVVQILDD226 pKa = 3.61 EE227 pKa = 4.79 KK228 pKa = 11.28 GEE230 pKa = 3.98 KK231 pKa = 9.96 SLNFMVHH238 pKa = 6.48 LGLIKK243 pKa = 10.42 RR244 pKa = 11.84 KK245 pKa = 8.58 VGRR248 pKa = 11.84 MYY250 pKa = 10.77 SVEE253 pKa = 3.94 YY254 pKa = 10.17 CKK256 pKa = 10.57 QKK258 pKa = 9.94 IEE260 pKa = 3.94 KK261 pKa = 8.72 MRR263 pKa = 11.84 LIFSLGLVGGISLHH277 pKa = 5.86 VNATGSISKK286 pKa = 8.54 TLASQLVFKK295 pKa = 11.15 RR296 pKa = 11.84 EE297 pKa = 3.37 ICYY300 pKa = 10.21 PLMDD304 pKa = 5.92 LNPHH308 pKa = 6.45 LNLVIWASSVEE319 pKa = 4.04 ITRR322 pKa = 11.84 VDD324 pKa = 4.73 AIFQPSLPGEE334 pKa = 3.75 FRR336 pKa = 11.84 YY337 pKa = 10.34 YY338 pKa = 10.39 PNIIAKK344 pKa = 9.88 GVGKK348 pKa = 10.33 IKK350 pKa = 10.11 QWNN353 pKa = 3.35
Molecular weight: 39.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.084
IPC2_protein 8.96
IPC_protein 8.843
Toseland 9.677
ProMoST 9.37
Dawson 9.911
Bjellqvist 9.589
Wikipedia 10.058
Rodwell 10.35
Grimsley 9.97
Solomon 9.926
Lehninger 9.897
Nozaki 9.75
DTASelect 9.56
Thurlkill 9.765
EMBOSS 10.101
Sillero 9.838
Patrickios 7.6
IPC_peptide 9.926
IPC2_peptide 8.141
IPC2.peptide.svr19 8.045
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
5386
199
2233
673.3
76.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.233 ± 0.466
1.355 ± 0.215
6.127 ± 0.398
5.997 ± 0.462
2.822 ± 0.52
5.217 ± 0.354
1.875 ± 0.159
9.005 ± 0.573
6.907 ± 0.465
9.543 ± 1.011
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.321 ± 0.184
6.424 ± 0.306
3.695 ± 0.254
4.475 ± 0.971
4.976 ± 0.443
8.708 ± 0.622
6.944 ± 0.854
4.846 ± 0.406
1.133 ± 0.223
3.398 ± 0.567
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here