Amycolatopsis antarctica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Amycolatopsis

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5495 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A263D1J2|A0A263D1J2_9PSEU Uncharacterized protein OS=Amycolatopsis antarctica OX=1854586 GN=CFN78_15040 PE=4 SV=1
MM1 pKa = 7.25KK2 pKa = 10.37RR3 pKa = 11.84ILAGTAAVVAAIAIPLLGTSVAQADD28 pKa = 3.63TGIAQSSPGDD38 pKa = 3.57DD39 pKa = 4.01GVWVLYY45 pKa = 10.61LDD47 pKa = 3.18QGYY50 pKa = 10.2IGWSGVASFADD61 pKa = 3.8ADD63 pKa = 3.86DD64 pKa = 4.1CAGYY68 pKa = 10.84GKK70 pKa = 10.3DD71 pKa = 5.05GEE73 pKa = 4.75DD74 pKa = 3.34EE75 pKa = 4.26GWWYY79 pKa = 10.61DD80 pKa = 3.82FACFDD85 pKa = 3.63ISDD88 pKa = 3.75GHH90 pKa = 5.93EE91 pKa = 3.95VPFDD95 pKa = 4.07PGHH98 pKa = 6.07RR99 pKa = 3.67

Molecular weight: 10.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A263CV24|A0A263CV24_9PSEU Uncharacterized protein OS=Amycolatopsis antarctica OX=1854586 GN=CFN78_27875 PE=4 SV=1
MM1 pKa = 7.75AISINKK7 pKa = 8.91KK8 pKa = 8.44RR9 pKa = 11.84TNKK12 pKa = 10.06SKK14 pKa = 10.23SASRR18 pKa = 11.84SRR20 pKa = 11.84RR21 pKa = 11.84QLRR24 pKa = 11.84IRR26 pKa = 11.84KK27 pKa = 8.71RR28 pKa = 11.84ISGTAVRR35 pKa = 11.84PRR37 pKa = 11.84LVVNRR42 pKa = 11.84SAA44 pKa = 4.34

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5495

0

5495

1828082

31

17227

332.7

35.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.428 ± 0.048

0.745 ± 0.013

6.136 ± 0.028

5.931 ± 0.026

2.785 ± 0.018

9.672 ± 0.031

2.202 ± 0.016

3.332 ± 0.024

1.801 ± 0.025

10.331 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.704 ± 0.015

1.816 ± 0.017

6.045 ± 0.031

2.65 ± 0.023

8.118 ± 0.038

5.163 ± 0.025

5.992 ± 0.02

8.795 ± 0.034

1.445 ± 0.013

1.907 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski