Amino acid dipepetide frequency for Amycolatopsis antarctica

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.391AlaAla: 20.391 ± 0.196
1.014AlaCys: 1.014 ± 0.028
8.625AlaAsp: 8.625 ± 0.09
9.998AlaGlu: 9.998 ± 0.091
3.349AlaPhe: 3.349 ± 0.049
14.259AlaGly: 14.259 ± 0.114
2.586AlaHis: 2.586 ± 0.044
4.226AlaIle: 4.226 ± 0.057
2.532AlaLys: 2.532 ± 0.055
13.718AlaLeu: 13.718 ± 0.126
2.484AlaMet: 2.484 ± 0.036
2.159AlaAsn: 2.159 ± 0.034
6.632AlaPro: 6.632 ± 0.075
3.468AlaGln: 3.468 ± 0.049
9.923AlaArg: 9.923 ± 0.091
5.837AlaSer: 5.837 ± 0.057
7.203AlaThr: 7.203 ± 0.071
11.931AlaVal: 11.931 ± 0.1
1.683AlaTrp: 1.683 ± 0.033
2.267AlaTyr: 2.267 ± 0.037
0.0AlaXaa: 0.0 ± 0.0
Cys
1.035CysAla: 1.035 ± 0.03
0.082CysCys: 0.082 ± 0.007
0.415CysAsp: 0.415 ± 0.015
0.402CysGlu: 0.402 ± 0.018
0.211CysPhe: 0.211 ± 0.01
0.899CysGly: 0.899 ± 0.027
0.182CysHis: 0.182 ± 0.01
0.149CysIle: 0.149 ± 0.01
0.102CysLys: 0.102 ± 0.008
0.665CysLeu: 0.665 ± 0.019
0.107CysMet: 0.107 ± 0.007
0.123CysAsn: 0.123 ± 0.008
0.459CysPro: 0.459 ± 0.017
0.141CysGln: 0.141 ± 0.01
0.594CysArg: 0.594 ± 0.018
0.469CysSer: 0.469 ± 0.021
0.462CysThr: 0.462 ± 0.019
0.691CysVal: 0.691 ± 0.023
0.115CysTrp: 0.115 ± 0.008
0.153CysTyr: 0.153 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
7.779AspAla: 7.779 ± 0.084
0.42AspCys: 0.42 ± 0.016
3.494AspAsp: 3.494 ± 0.053
4.267AspGlu: 4.267 ± 0.053
1.581AspPhe: 1.581 ± 0.027
6.587AspGly: 6.587 ± 0.074
1.32AspHis: 1.32 ± 0.028
1.932AspIle: 1.932 ± 0.034
0.994AspLys: 0.994 ± 0.024
6.397AspLeu: 6.397 ± 0.07
0.802AspMet: 0.802 ± 0.022
0.956AspAsn: 0.956 ± 0.028
4.675AspPro: 4.675 ± 0.064
1.609AspGln: 1.609 ± 0.038
5.075AspArg: 5.075 ± 0.061
2.843AspSer: 2.843 ± 0.049
3.367AspThr: 3.367 ± 0.046
5.074AspVal: 5.074 ± 0.061
1.015AspTrp: 1.015 ± 0.026
1.17AspTyr: 1.17 ± 0.032
0.0AspXaa: 0.0 ± 0.0
Glu
6.739GluAla: 6.739 ± 0.07
0.374GluCys: 0.374 ± 0.015
2.954GluAsp: 2.954 ± 0.04
2.956GluGlu: 2.956 ± 0.044
1.837GluPhe: 1.837 ± 0.032
4.063GluGly: 4.063 ± 0.049
1.784GluHis: 1.784 ± 0.034
2.436GluIle: 2.436 ± 0.043
1.212GluLys: 1.212 ± 0.027
7.92GluLeu: 7.92 ± 0.088
0.886GluMet: 0.886 ± 0.024
1.202GluAsn: 1.202 ± 0.03
3.739GluPro: 3.739 ± 0.053
2.582GluGln: 2.582 ± 0.054
5.754GluArg: 5.754 ± 0.054
2.841GluSer: 2.841 ± 0.04
3.005GluThr: 3.005 ± 0.041
5.147GluVal: 5.147 ± 0.053
0.808GluTrp: 0.808 ± 0.021
1.069GluTyr: 1.069 ± 0.025
0.0GluXaa: 0.0 ± 0.0
Phe
4.064PheAla: 4.064 ± 0.051
0.24PheCys: 0.24 ± 0.014
2.265PheAsp: 2.265 ± 0.048
1.457PheGlu: 1.457 ± 0.032
0.876PhePhe: 0.876 ± 0.024
3.295PheGly: 3.295 ± 0.05
0.625PheHis: 0.625 ± 0.016
0.664PheIle: 0.664 ± 0.021
0.345PheLys: 0.345 ± 0.014
2.543PheLeu: 2.543 ± 0.04
0.376PheMet: 0.376 ± 0.016
0.518PheAsn: 0.518 ± 0.019
1.367PhePro: 1.367 ± 0.028
0.617PheGln: 0.617 ± 0.02
1.828PheArg: 1.828 ± 0.033
1.433PheSer: 1.433 ± 0.028
1.912PheThr: 1.912 ± 0.034
2.429PheVal: 2.429 ± 0.042
0.399PheTrp: 0.399 ± 0.017
0.598PheTyr: 0.598 ± 0.017
0.0PheXaa: 0.0 ± 0.0
Gly
11.164GlyAla: 11.164 ± 0.097
0.82GlyCys: 0.82 ± 0.026
5.344GlyAsp: 5.344 ± 0.06
5.999GlyGlu: 5.999 ± 0.061
3.019GlyPhe: 3.019 ± 0.046
9.101GlyGly: 9.101 ± 0.114
2.266GlyHis: 2.266 ± 0.044
3.711GlyIle: 3.711 ± 0.043
2.306GlyLys: 2.306 ± 0.042
9.412GlyLeu: 9.412 ± 0.08
2.186GlyMet: 2.186 ± 0.041
1.934GlyAsn: 1.934 ± 0.045
5.053GlyPro: 5.053 ± 0.068
2.817GlyGln: 2.817 ± 0.051
7.51GlyArg: 7.51 ± 0.071
5.449GlySer: 5.449 ± 0.059
6.401GlyThr: 6.401 ± 0.068
8.256GlyVal: 8.256 ± 0.088
1.672GlyTrp: 1.672 ± 0.035
2.303GlyTyr: 2.303 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
2.581HisAla: 2.581 ± 0.04
0.208HisCys: 0.208 ± 0.012
1.328HisAsp: 1.328 ± 0.027
1.313HisGlu: 1.313 ± 0.028
0.578HisPhe: 0.578 ± 0.019
2.444HisGly: 2.444 ± 0.039
0.661HisHis: 0.661 ± 0.025
0.604HisIle: 0.604 ± 0.018
0.292HisLys: 0.292 ± 0.012
2.235HisLeu: 2.235 ± 0.04
0.28HisMet: 0.28 ± 0.013
0.39HisAsn: 0.39 ± 0.015
1.66HisPro: 1.66 ± 0.036
0.656HisGln: 0.656 ± 0.019
2.054HisArg: 2.054 ± 0.038
1.061HisSer: 1.061 ± 0.025
1.186HisThr: 1.186 ± 0.029
1.682HisVal: 1.682 ± 0.035
0.338HisTrp: 0.338 ± 0.014
0.47HisTyr: 0.47 ± 0.016
0.0HisXaa: 0.0 ± 0.0
Ile
5.3IleAla: 5.3 ± 0.069
0.238IleCys: 0.238 ± 0.012
2.508IleAsp: 2.508 ± 0.041
2.168IleGlu: 2.168 ± 0.035
0.723IlePhe: 0.723 ± 0.02
4.041IleGly: 4.041 ± 0.063
0.581IleHis: 0.581 ± 0.017
0.905IleIle: 0.905 ± 0.024
0.585IleLys: 0.585 ± 0.021
2.367IleLeu: 2.367 ± 0.039
0.428IleMet: 0.428 ± 0.018
0.716IleAsn: 0.716 ± 0.023
1.807IlePro: 1.807 ± 0.032
0.662IleGln: 0.662 ± 0.021
2.328IleArg: 2.328 ± 0.038
1.758IleSer: 1.758 ± 0.031
2.192IleThr: 2.192 ± 0.039
3.104IleVal: 3.104 ± 0.047
0.362IleTrp: 0.362 ± 0.016
0.548IleTyr: 0.548 ± 0.019
0.0IleXaa: 0.0 ± 0.0
Lys
2.261LysAla: 2.261 ± 0.049
0.089LysCys: 0.089 ± 0.008
0.999LysAsp: 0.999 ± 0.028
0.881LysGlu: 0.881 ± 0.023
0.452LysPhe: 0.452 ± 0.017
1.428LysGly: 1.428 ± 0.033
0.404LysHis: 0.404 ± 0.013
0.793LysIle: 0.793 ± 0.026
0.578LysLys: 0.578 ± 0.025
1.915LysLeu: 1.915 ± 0.038
0.343LysMet: 0.343 ± 0.013
0.41LysAsn: 0.41 ± 0.016
1.149LysPro: 1.149 ± 0.029
0.656LysGln: 0.656 ± 0.021
1.397LysArg: 1.397 ± 0.031
0.986LysSer: 0.986 ± 0.029
1.055LysThr: 1.055 ± 0.026
1.638LysVal: 1.638 ± 0.035
0.213LysTrp: 0.213 ± 0.011
0.363LysTyr: 0.363 ± 0.015
0.0LysXaa: 0.0 ± 0.0
Leu
15.718LeuAla: 15.718 ± 0.142
0.798LeuCys: 0.798 ± 0.024
7.276LeuAsp: 7.276 ± 0.077
4.446LeuGlu: 4.446 ± 0.055
2.679LeuPhe: 2.679 ± 0.039
9.889LeuGly: 9.889 ± 0.089
2.208LeuHis: 2.208 ± 0.044
3.208LeuIle: 3.208 ± 0.052
1.362LeuLys: 1.362 ± 0.034
11.252LeuLeu: 11.252 ± 0.138
1.359LeuMet: 1.359 ± 0.03
1.6LeuAsn: 1.6 ± 0.031
6.523LeuPro: 6.523 ± 0.078
1.832LeuGln: 1.832 ± 0.036
9.293LeuArg: 9.293 ± 0.087
5.43LeuSer: 5.43 ± 0.058
6.437LeuThr: 6.437 ± 0.061
9.159LeuVal: 9.159 ± 0.081
1.207LeuTrp: 1.207 ± 0.027
1.636LeuTyr: 1.636 ± 0.035
0.0LeuXaa: 0.0 ± 0.0
Met
2.08MetAla: 2.08 ± 0.033
0.122MetCys: 0.122 ± 0.008
0.839MetAsp: 0.839 ± 0.02
0.673MetGlu: 0.673 ± 0.019
0.527MetPhe: 0.527 ± 0.019
1.171MetGly: 1.171 ± 0.026
0.34MetHis: 0.34 ± 0.013
0.712MetIle: 0.712 ± 0.02
0.31MetLys: 0.31 ± 0.014
1.843MetLeu: 1.843 ± 0.032
0.264MetMet: 0.264 ± 0.011
0.402MetAsn: 0.402 ± 0.013
1.16MetPro: 1.16 ± 0.026
0.387MetGln: 0.387 ± 0.015
1.496MetArg: 1.496 ± 0.029
1.356MetSer: 1.356 ± 0.028
1.509MetThr: 1.509 ± 0.034
1.359MetVal: 1.359 ± 0.029
0.21MetTrp: 0.21 ± 0.013
0.284MetTyr: 0.284 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.322AsnAla: 2.322 ± 0.04
0.167AsnCys: 0.167 ± 0.011
0.99AsnAsp: 0.99 ± 0.026
0.944AsnGlu: 0.944 ± 0.025
0.493AsnPhe: 0.493 ± 0.018
1.929AsnGly: 1.929 ± 0.036
0.398AsnHis: 0.398 ± 0.015
0.607AsnIle: 0.607 ± 0.02
0.351AsnLys: 0.351 ± 0.018
1.814AsnLeu: 1.814 ± 0.039
0.301AsnMet: 0.301 ± 0.014
0.393AsnAsn: 0.393 ± 0.014
1.412AsnPro: 1.412 ± 0.033
0.534AsnGln: 0.534 ± 0.017
1.29AsnArg: 1.29 ± 0.03
0.924AsnSer: 0.924 ± 0.025
1.104AsnThr: 1.104 ± 0.024
1.467AsnVal: 1.467 ± 0.029
0.277AsnTrp: 0.277 ± 0.013
0.436AsnTyr: 0.436 ± 0.018
0.0AsnXaa: 0.0 ± 0.0
Pro
8.97ProAla: 8.97 ± 0.1
0.342ProCys: 0.342 ± 0.013
4.47ProAsp: 4.47 ± 0.058
4.634ProGlu: 4.634 ± 0.054
1.534ProPhe: 1.534 ± 0.03
6.959ProGly: 6.959 ± 0.087
1.203ProHis: 1.203 ± 0.028
1.632ProIle: 1.632 ± 0.034
1.075ProLys: 1.075 ± 0.025
4.963ProLeu: 4.963 ± 0.064
1.036ProMet: 1.036 ± 0.024
1.011ProAsn: 1.011 ± 0.027
3.697ProPro: 3.697 ± 0.075
1.487ProGln: 1.487 ± 0.036
3.957ProArg: 3.957 ± 0.049
3.166ProSer: 3.166 ± 0.044
2.845ProThr: 2.845 ± 0.047
5.586ProVal: 5.586 ± 0.069
0.828ProTrp: 0.828 ± 0.022
1.054ProTyr: 1.054 ± 0.024
0.0ProXaa: 0.0 ± 0.0
Gln
3.596GlnAla: 3.596 ± 0.054
0.167GlnCys: 0.167 ± 0.011
1.34GlnAsp: 1.34 ± 0.034
1.252GlnGlu: 1.252 ± 0.029
0.731GlnPhe: 0.731 ± 0.024
2.026GlnGly: 2.026 ± 0.04
0.643GlnHis: 0.643 ± 0.02
1.043GlnIle: 1.043 ± 0.024
0.447GlnLys: 0.447 ± 0.016
2.828GlnLeu: 2.828 ± 0.041
0.395GlnMet: 0.395 ± 0.015
0.523GlnAsn: 0.523 ± 0.018
1.736GlnPro: 1.736 ± 0.052
1.26GlnGln: 1.26 ± 0.044
2.525GlnArg: 2.525 ± 0.045
1.263GlnSer: 1.263 ± 0.032
1.224GlnThr: 1.224 ± 0.029
2.532GlnVal: 2.532 ± 0.041
0.424GlnTrp: 0.424 ± 0.015
0.548GlnTyr: 0.548 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
9.807ArgAla: 9.807 ± 0.082
0.585ArgCys: 0.585 ± 0.02
4.506ArgAsp: 4.506 ± 0.047
5.027ArgGlu: 5.027 ± 0.064
2.523ArgPhe: 2.523 ± 0.04
5.978ArgGly: 5.978 ± 0.069
1.961ArgHis: 1.961 ± 0.033
3.239ArgIle: 3.239 ± 0.044
1.637ArgLys: 1.637 ± 0.039
8.528ArgLeu: 8.528 ± 0.087
1.916ArgMet: 1.916 ± 0.035
1.494ArgAsn: 1.494 ± 0.032
4.912ArgPro: 4.912 ± 0.065
2.308ArgGln: 2.308 ± 0.046
7.753ArgArg: 7.753 ± 0.079
4.35ArgSer: 4.35 ± 0.05
5.239ArgThr: 5.239 ± 0.06
6.132ArgVal: 6.132 ± 0.061
1.436ArgTrp: 1.436 ± 0.037
1.853ArgTyr: 1.853 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
6.92SerAla: 6.92 ± 0.064
0.405SerCys: 0.405 ± 0.015
2.714SerAsp: 2.714 ± 0.06
2.593SerGlu: 2.593 ± 0.038
1.542SerPhe: 1.542 ± 0.03
6.263SerGly: 6.263 ± 0.074
0.903SerHis: 0.903 ± 0.022
1.663SerIle: 1.663 ± 0.033
0.95SerLys: 0.95 ± 0.025
4.69SerLeu: 4.69 ± 0.049
1.084SerMet: 1.084 ± 0.022
0.886SerAsn: 0.886 ± 0.026
3.148SerPro: 3.148 ± 0.051
1.176SerGln: 1.176 ± 0.028
3.879SerArg: 3.879 ± 0.045
2.849SerSer: 2.849 ± 0.061
3.392SerThr: 3.392 ± 0.044
4.553SerVal: 4.553 ± 0.054
0.918SerTrp: 0.918 ± 0.024
1.106SerTyr: 1.106 ± 0.026
0.0SerXaa: 0.0 ± 0.0
Thr
8.182ThrAla: 8.182 ± 0.076
0.392ThrCys: 0.392 ± 0.016
3.464ThrAsp: 3.464 ± 0.055
3.709ThrGlu: 3.709 ± 0.049
1.619ThrPhe: 1.619 ± 0.03
6.802ThrGly: 6.802 ± 0.067
1.089ThrHis: 1.089 ± 0.023
2.014ThrIle: 2.014 ± 0.033
1.032ThrLys: 1.032 ± 0.023
5.725ThrLeu: 5.725 ± 0.062
1.068ThrMet: 1.068 ± 0.026
0.993ThrAsn: 0.993 ± 0.024
3.821ThrPro: 3.821 ± 0.051
1.193ThrGln: 1.193 ± 0.027
3.843ThrArg: 3.843 ± 0.049
3.142ThrSer: 3.142 ± 0.042
3.701ThrThr: 3.701 ± 0.056
6.207ThrVal: 6.207 ± 0.067
0.826ThrTrp: 0.826 ± 0.021
1.096ThrTyr: 1.096 ± 0.026
0.0ThrXaa: 0.0 ± 0.0
Val
11.527ValAla: 11.527 ± 0.098
0.694ValCys: 0.694 ± 0.022
5.884ValAsp: 5.884 ± 0.067
4.887ValGlu: 4.887 ± 0.065
2.445ValPhe: 2.445 ± 0.039
6.733ValGly: 6.733 ± 0.067
2.069ValHis: 2.069 ± 0.037
2.826ValIle: 2.826 ± 0.048
1.354ValLys: 1.354 ± 0.032
10.532ValLeu: 10.532 ± 0.098
1.217ValMet: 1.217 ± 0.027
1.655ValAsn: 1.655 ± 0.035
5.522ValPro: 5.522 ± 0.073
1.946ValGln: 1.946 ± 0.037
7.542ValArg: 7.542 ± 0.081
4.586ValSer: 4.586 ± 0.049
5.655ValThr: 5.655 ± 0.063
8.378ValVal: 8.378 ± 0.092
1.049ValTrp: 1.049 ± 0.026
1.451ValTyr: 1.451 ± 0.03
0.0ValXaa: 0.0 ± 0.0
Trp
1.543TrpAla: 1.543 ± 0.031
0.138TrpCys: 0.138 ± 0.009
0.735TrpAsp: 0.735 ± 0.02
0.639TrpGlu: 0.639 ± 0.018
0.527TrpPhe: 0.527 ± 0.02
0.934TrpGly: 0.934 ± 0.025
0.369TrpHis: 0.369 ± 0.014
0.539TrpIle: 0.539 ± 0.021
0.274TrpLys: 0.274 ± 0.012
1.75TrpLeu: 1.75 ± 0.034
0.292TrpMet: 0.292 ± 0.014
0.409TrpAsn: 0.409 ± 0.013
0.849TrpPro: 0.849 ± 0.025
0.597TrpGln: 0.597 ± 0.018
1.345TrpArg: 1.345 ± 0.03
0.893TrpSer: 0.893 ± 0.025
0.984TrpThr: 0.984 ± 0.022
1.015TrpVal: 1.015 ± 0.024
0.345TrpTrp: 0.345 ± 0.014
0.27TrpTyr: 0.27 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.303TyrAla: 2.303 ± 0.035
0.16TyrCys: 0.16 ± 0.008
1.206TyrAsp: 1.206 ± 0.027
1.062TyrGlu: 1.062 ± 0.025
0.604TyrPhe: 0.604 ± 0.021
1.923TyrGly: 1.923 ± 0.034
0.429TyrHis: 0.429 ± 0.013
0.417TyrIle: 0.417 ± 0.016
0.272TyrLys: 0.272 ± 0.013
2.219TyrLeu: 2.219 ± 0.043
0.218TyrMet: 0.218 ± 0.012
0.381TyrAsn: 0.381 ± 0.017
1.126TyrPro: 1.126 ± 0.026
0.575TyrGln: 0.575 ± 0.019
1.799TyrArg: 1.799 ± 0.032
0.996TyrSer: 0.996 ± 0.026
1.043TyrThr: 1.043 ± 0.026
1.616TyrVal: 1.616 ± 0.033
0.324TyrTrp: 0.324 ± 0.012
0.399TyrTyr: 0.399 ± 0.016
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5495 proteins (1828082 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski