Rheinheimera sp. D18

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Chromatiaceae; Rheinheimera; unclassified Rheinheimera

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2946 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P6VT41|A0A4P6VT41_9GAMM DUF1289 domain-containing protein OS=Rheinheimera sp. D18 OX=2545632 GN=E0Z06_12310 PE=4 SV=1
MM1 pKa = 7.81KK2 pKa = 10.19IGLFYY7 pKa = 11.11GSTTCYY13 pKa = 10.16TEE15 pKa = 3.93IVAEE19 pKa = 4.75KK20 pKa = 9.74IQALIGADD28 pKa = 3.31TVEE31 pKa = 4.51LNNIKK36 pKa = 10.22HH37 pKa = 5.97IPLSKK42 pKa = 10.02MADD45 pKa = 3.47YY46 pKa = 7.75DD47 pKa = 3.88TLIFGLSTWDD57 pKa = 3.32FGEE60 pKa = 4.16IQEE63 pKa = 4.55DD64 pKa = 4.44WEE66 pKa = 4.35AHH68 pKa = 5.64WDD70 pKa = 4.24DD71 pKa = 4.43ISSVDD76 pKa = 4.2LNGKK80 pKa = 9.5IIAIYY85 pKa = 10.64GMGDD89 pKa = 3.26QIGYY93 pKa = 10.43AEE95 pKa = 4.23WFIDD99 pKa = 4.04AVGMLHH105 pKa = 7.43DD106 pKa = 5.13VIAPQQCSRR115 pKa = 11.84IGFWPSAGYY124 pKa = 10.75DD125 pKa = 4.21FIASKK130 pKa = 11.03ALTEE134 pKa = 4.71DD135 pKa = 3.77GEE137 pKa = 4.52LFYY140 pKa = 11.5GLALDD145 pKa = 6.2DD146 pKa = 4.35EE147 pKa = 4.64NQYY150 pKa = 11.82DD151 pKa = 4.1LTDD154 pKa = 3.2EE155 pKa = 4.69RR156 pKa = 11.84LNDD159 pKa = 3.64WVSQILQEE167 pKa = 4.73IAQQLL172 pKa = 3.54

Molecular weight: 19.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V1A7A8|A0A4V1A7A8_9GAMM Thiol reductant ABC exporter subunit CydD OS=Rheinheimera sp. D18 OX=2545632 GN=cydD PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.98RR4 pKa = 11.84TFQPSVLKK12 pKa = 10.46RR13 pKa = 11.84KK14 pKa = 9.37RR15 pKa = 11.84NHH17 pKa = 5.37GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.3NGRR29 pKa = 11.84AVLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.25GRR40 pKa = 11.84QRR42 pKa = 11.84LSAA45 pKa = 4.04

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2946

0

2946

985838

37

1938

334.6

37.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.994 ± 0.052

0.964 ± 0.017

5.173 ± 0.03

5.281 ± 0.042

3.97 ± 0.03

6.435 ± 0.045

2.188 ± 0.021

5.985 ± 0.028

4.791 ± 0.036

11.544 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.353 ± 0.02

4.184 ± 0.029

3.972 ± 0.023

5.815 ± 0.057

4.751 ± 0.027

6.295 ± 0.031

5.255 ± 0.028

6.773 ± 0.037

1.246 ± 0.02

3.03 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski