Amino acid dipepetide frequency for Rheinheimera sp. D18

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.474AlaAla: 10.474 ± 0.145
1.01AlaCys: 1.01 ± 0.034
5.869AlaAsp: 5.869 ± 0.077
6.654AlaGlu: 6.654 ± 0.094
3.55AlaPhe: 3.55 ± 0.06
6.702AlaGly: 6.702 ± 0.1
1.671AlaHis: 1.671 ± 0.041
6.434AlaIle: 6.434 ± 0.075
5.175AlaLys: 5.175 ± 0.078
11.951AlaLeu: 11.951 ± 0.108
2.674AlaMet: 2.674 ± 0.057
4.088AlaAsn: 4.088 ± 0.078
3.29AlaPro: 3.29 ± 0.067
5.128AlaGln: 5.128 ± 0.079
4.22AlaArg: 4.22 ± 0.07
5.7AlaSer: 5.7 ± 0.083
4.868AlaThr: 4.868 ± 0.075
6.933AlaVal: 6.933 ± 0.091
0.963AlaTrp: 0.963 ± 0.033
2.587AlaTyr: 2.587 ± 0.054
0.0AlaXaa: 0.0 ± 0.0
Cys
0.904CysAla: 0.904 ± 0.034
0.168CysCys: 0.168 ± 0.013
0.525CysAsp: 0.525 ± 0.023
0.458CysGlu: 0.458 ± 0.024
0.438CysPhe: 0.438 ± 0.021
0.795CysGly: 0.795 ± 0.03
0.297CysHis: 0.297 ± 0.016
0.533CysIle: 0.533 ± 0.025
0.343CysLys: 0.343 ± 0.018
0.951CysLeu: 0.951 ± 0.032
0.169CysMet: 0.169 ± 0.012
0.381CysAsn: 0.381 ± 0.021
0.452CysPro: 0.452 ± 0.026
0.527CysGln: 0.527 ± 0.024
0.489CysArg: 0.489 ± 0.024
0.71CysSer: 0.71 ± 0.028
0.456CysThr: 0.456 ± 0.022
0.554CysVal: 0.554 ± 0.026
0.128CysTrp: 0.128 ± 0.011
0.355CysTyr: 0.355 ± 0.02
0.0CysXaa: 0.0 ± 0.0
Asp
5.027AspAla: 5.027 ± 0.07
0.456AspCys: 0.456 ± 0.023
2.887AspAsp: 2.887 ± 0.067
3.177AspGlu: 3.177 ± 0.059
2.226AspPhe: 2.226 ± 0.052
3.52AspGly: 3.52 ± 0.074
0.878AspHis: 0.878 ± 0.035
4.131AspIle: 4.131 ± 0.074
2.94AspLys: 2.94 ± 0.062
4.834AspLeu: 4.834 ± 0.075
1.271AspMet: 1.271 ± 0.037
2.573AspAsn: 2.573 ± 0.045
1.804AspPro: 1.804 ± 0.044
1.745AspGln: 1.745 ± 0.045
1.98AspArg: 1.98 ± 0.049
3.105AspSer: 3.105 ± 0.052
2.825AspThr: 2.825 ± 0.054
3.616AspVal: 3.616 ± 0.06
0.796AspTrp: 0.796 ± 0.025
1.941AspTyr: 1.941 ± 0.04
0.0AspXaa: 0.0 ± 0.0
Glu
4.508GluAla: 4.508 ± 0.081
0.362GluCys: 0.362 ± 0.017
2.085GluAsp: 2.085 ± 0.051
2.275GluGlu: 2.275 ± 0.062
2.221GluPhe: 2.221 ± 0.045
2.609GluGly: 2.609 ± 0.06
1.585GluHis: 1.585 ± 0.043
3.023GluIle: 3.023 ± 0.062
2.807GluLys: 2.807 ± 0.065
7.316GluLeu: 7.316 ± 0.105
1.256GluMet: 1.256 ± 0.044
1.93GluAsn: 1.93 ± 0.04
2.056GluPro: 2.056 ± 0.055
5.203GluGln: 5.203 ± 0.106
3.233GluArg: 3.233 ± 0.063
2.309GluSer: 2.309 ± 0.054
2.48GluThr: 2.48 ± 0.051
3.461GluVal: 3.461 ± 0.061
0.514GluTrp: 0.514 ± 0.025
1.579GluTyr: 1.579 ± 0.04
0.0GluXaa: 0.0 ± 0.0
Phe
4.134PheAla: 4.134 ± 0.074
0.49PheCys: 0.49 ± 0.023
2.524PheAsp: 2.524 ± 0.055
1.935PheGlu: 1.935 ± 0.045
1.628PhePhe: 1.628 ± 0.05
2.97PheGly: 2.97 ± 0.063
0.734PheHis: 0.734 ± 0.03
2.671PheIle: 2.671 ± 0.051
1.77PheLys: 1.77 ± 0.047
3.219PheLeu: 3.219 ± 0.064
0.962PheMet: 0.962 ± 0.037
2.185PheAsn: 2.185 ± 0.049
1.274PhePro: 1.274 ± 0.039
1.209PheGln: 1.209 ± 0.035
1.555PheArg: 1.555 ± 0.039
3.518PheSer: 3.518 ± 0.073
2.342PheThr: 2.342 ± 0.053
2.627PheVal: 2.627 ± 0.059
0.582PheTrp: 0.582 ± 0.031
1.373PheTyr: 1.373 ± 0.042
0.0PheXaa: 0.0 ± 0.0
Gly
5.481GlyAla: 5.481 ± 0.108
0.813GlyCys: 0.813 ± 0.034
3.265GlyAsp: 3.265 ± 0.058
3.601GlyGlu: 3.601 ± 0.07
3.174GlyPhe: 3.174 ± 0.064
4.383GlyGly: 4.383 ± 0.079
1.399GlyHis: 1.399 ± 0.042
4.393GlyIle: 4.393 ± 0.074
3.779GlyLys: 3.779 ± 0.066
6.667GlyLeu: 6.667 ± 0.088
1.786GlyMet: 1.786 ± 0.044
2.578GlyAsn: 2.578 ± 0.061
1.619GlyPro: 1.619 ± 0.043
2.835GlyGln: 2.835 ± 0.05
3.111GlyArg: 3.111 ± 0.063
4.014GlySer: 4.014 ± 0.069
3.115GlyThr: 3.115 ± 0.068
4.92GlyVal: 4.92 ± 0.078
0.95GlyTrp: 0.95 ± 0.037
2.468GlyTyr: 2.468 ± 0.055
0.0GlyXaa: 0.0 ± 0.0
His
1.824HisAla: 1.824 ± 0.048
0.315HisCys: 0.315 ± 0.017
1.026HisAsp: 1.026 ± 0.032
0.968HisGlu: 0.968 ± 0.033
1.017HisPhe: 1.017 ± 0.035
1.564HisGly: 1.564 ± 0.039
0.59HisHis: 0.59 ± 0.028
1.436HisIle: 1.436 ± 0.037
1.023HisLys: 1.023 ± 0.032
2.238HisLeu: 2.238 ± 0.049
0.435HisMet: 0.435 ± 0.024
1.005HisAsn: 1.005 ± 0.032
1.071HisPro: 1.071 ± 0.034
1.231HisGln: 1.231 ± 0.037
0.981HisArg: 0.981 ± 0.034
1.555HisSer: 1.555 ± 0.047
1.054HisThr: 1.054 ± 0.031
1.18HisVal: 1.18 ± 0.031
0.457HisTrp: 0.457 ± 0.024
0.909HisTyr: 0.909 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
6.958IleAla: 6.958 ± 0.101
0.619IleCys: 0.619 ± 0.027
3.682IleAsp: 3.682 ± 0.058
3.547IleGlu: 3.547 ± 0.073
2.003IlePhe: 2.003 ± 0.051
4.174IleGly: 4.174 ± 0.076
1.031IleHis: 1.031 ± 0.034
3.364IleIle: 3.364 ± 0.064
3.183IleLys: 3.183 ± 0.06
5.248IleLeu: 5.248 ± 0.081
1.22IleMet: 1.22 ± 0.037
3.083IleAsn: 3.083 ± 0.056
2.431IlePro: 2.431 ± 0.051
2.196IleGln: 2.196 ± 0.056
2.729IleArg: 2.729 ± 0.058
4.608IleSer: 4.608 ± 0.067
3.709IleThr: 3.709 ± 0.061
3.859IleVal: 3.859 ± 0.068
0.63IleTrp: 0.63 ± 0.024
1.58IleTyr: 1.58 ± 0.037
0.0IleXaa: 0.0 ± 0.0
Lys
5.187LysAla: 5.187 ± 0.084
0.268LysCys: 0.268 ± 0.018
2.299LysAsp: 2.299 ± 0.062
2.163LysGlu: 2.163 ± 0.064
1.45LysPhe: 1.45 ± 0.036
2.724LysGly: 2.724 ± 0.061
1.253LysHis: 1.253 ± 0.035
2.546LysIle: 2.546 ± 0.051
2.26LysLys: 2.26 ± 0.055
5.843LysLeu: 5.843 ± 0.079
1.188LysMet: 1.188 ± 0.034
1.827LysAsn: 1.827 ± 0.05
2.453LysPro: 2.453 ± 0.055
3.431LysGln: 3.431 ± 0.064
2.605LysArg: 2.605 ± 0.06
2.369LysSer: 2.369 ± 0.05
2.806LysThr: 2.806 ± 0.062
3.625LysVal: 3.625 ± 0.061
0.45LysTrp: 0.45 ± 0.022
1.169LysTyr: 1.169 ± 0.031
0.0LysXaa: 0.0 ± 0.0
Leu
12.873LeuAla: 12.873 ± 0.152
1.2LeuCys: 1.2 ± 0.032
5.671LeuAsp: 5.671 ± 0.078
5.483LeuGlu: 5.483 ± 0.08
4.3LeuPhe: 4.3 ± 0.081
6.996LeuGly: 6.996 ± 0.095
2.38LeuHis: 2.38 ± 0.053
6.16LeuIle: 6.16 ± 0.097
5.257LeuLys: 5.257 ± 0.076
13.744LeuLeu: 13.744 ± 0.181
2.629LeuMet: 2.629 ± 0.058
4.903LeuAsn: 4.903 ± 0.076
5.493LeuPro: 5.493 ± 0.092
6.476LeuGln: 6.476 ± 0.132
5.193LeuArg: 5.193 ± 0.085
8.071LeuSer: 8.071 ± 0.115
6.888LeuThr: 6.888 ± 0.095
7.467LeuVal: 7.467 ± 0.105
1.218LeuTrp: 1.218 ± 0.042
3.039LeuTyr: 3.039 ± 0.059
0.0LeuXaa: 0.0 ± 0.0
Met
2.625MetAla: 2.625 ± 0.055
0.174MetCys: 0.174 ± 0.013
0.913MetAsp: 0.913 ± 0.031
0.86MetGlu: 0.86 ± 0.035
0.814MetPhe: 0.814 ± 0.027
1.332MetGly: 1.332 ± 0.048
0.541MetHis: 0.541 ± 0.024
1.152MetIle: 1.152 ± 0.038
1.02MetLys: 1.02 ± 0.027
3.324MetLeu: 3.324 ± 0.062
0.575MetMet: 0.575 ± 0.024
0.817MetAsn: 0.817 ± 0.029
1.29MetPro: 1.29 ± 0.035
1.642MetGln: 1.642 ± 0.041
1.279MetArg: 1.279 ± 0.04
1.609MetSer: 1.609 ± 0.039
1.33MetThr: 1.33 ± 0.038
1.512MetVal: 1.512 ± 0.044
0.201MetTrp: 0.201 ± 0.016
0.514MetTyr: 0.514 ± 0.029
0.0MetXaa: 0.0 ± 0.0
Asn
4.014AsnAla: 4.014 ± 0.07
0.418AsnCys: 0.418 ± 0.021
2.13AsnAsp: 2.13 ± 0.049
2.093AsnGlu: 2.093 ± 0.052
1.541AsnPhe: 1.541 ± 0.041
2.891AsnGly: 2.891 ± 0.066
0.847AsnHis: 0.847 ± 0.027
2.75AsnIle: 2.75 ± 0.05
2.124AsnLys: 2.124 ± 0.041
4.107AsnLeu: 4.107 ± 0.064
1.025AsnMet: 1.025 ± 0.029
2.068AsnAsn: 2.068 ± 0.052
1.847AsnPro: 1.847 ± 0.052
2.058AsnGln: 2.058 ± 0.043
2.032AsnArg: 2.032 ± 0.043
2.78AsnSer: 2.78 ± 0.064
2.381AsnThr: 2.381 ± 0.051
2.595AsnVal: 2.595 ± 0.053
0.745AsnTrp: 0.745 ± 0.027
1.399AsnTyr: 1.399 ± 0.042
0.0AsnXaa: 0.0 ± 0.0
Pro
4.081ProAla: 4.081 ± 0.077
0.326ProCys: 0.326 ± 0.021
2.394ProAsp: 2.394 ± 0.051
3.012ProGlu: 3.012 ± 0.06
1.593ProPhe: 1.593 ± 0.046
2.394ProGly: 2.394 ± 0.052
0.802ProHis: 0.802 ± 0.028
2.273ProIle: 2.273 ± 0.05
1.804ProLys: 1.804 ± 0.039
4.573ProLeu: 4.573 ± 0.082
0.945ProMet: 0.945 ± 0.031
1.64ProAsn: 1.64 ± 0.04
1.175ProPro: 1.175 ± 0.042
1.954ProGln: 1.954 ± 0.051
1.468ProArg: 1.468 ± 0.044
2.274ProSer: 2.274 ± 0.046
2.029ProThr: 2.029 ± 0.046
3.264ProVal: 3.264 ± 0.061
0.527ProTrp: 0.527 ± 0.023
1.194ProTyr: 1.194 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
5.58GlnAla: 5.58 ± 0.087
0.406GlnCys: 0.406 ± 0.02
2.102GlnAsp: 2.102 ± 0.046
2.018GlnGlu: 2.018 ± 0.05
2.193GlnPhe: 2.193 ± 0.05
3.062GlnGly: 3.062 ± 0.061
2.022GlnHis: 2.022 ± 0.056
2.706GlnIle: 2.706 ± 0.052
2.108GlnLys: 2.108 ± 0.049
8.769GlnLeu: 8.769 ± 0.16
1.106GlnMet: 1.106 ± 0.032
1.762GlnAsn: 1.762 ± 0.046
2.408GlnPro: 2.408 ± 0.055
7.304GlnGln: 7.304 ± 0.201
3.268GlnArg: 3.268 ± 0.066
2.89GlnSer: 2.89 ± 0.059
2.371GlnThr: 2.371 ± 0.054
3.49GlnVal: 3.49 ± 0.07
0.803GlnTrp: 0.803 ± 0.033
1.782GlnTyr: 1.782 ± 0.041
0.0GlnXaa: 0.0 ± 0.0
Arg
3.843ArgAla: 3.843 ± 0.06
0.452ArgCys: 0.452 ± 0.022
2.481ArgAsp: 2.481 ± 0.054
2.709ArgGlu: 2.709 ± 0.057
2.334ArgPhe: 2.334 ± 0.056
2.68ArgGly: 2.68 ± 0.06
1.153ArgHis: 1.153 ± 0.035
3.009ArgIle: 3.009 ± 0.053
2.203ArgLys: 2.203 ± 0.045
5.796ArgLeu: 5.796 ± 0.078
1.106ArgMet: 1.106 ± 0.035
1.956ArgAsn: 1.956 ± 0.044
1.661ArgPro: 1.661 ± 0.043
2.945ArgGln: 2.945 ± 0.066
2.544ArgArg: 2.544 ± 0.064
2.801ArgSer: 2.801 ± 0.057
2.143ArgThr: 2.143 ± 0.046
3.072ArgVal: 3.072 ± 0.056
0.666ArgTrp: 0.666 ± 0.028
1.955ArgTyr: 1.955 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
6.564SerAla: 6.564 ± 0.075
0.618SerCys: 0.618 ± 0.028
3.212SerAsp: 3.212 ± 0.054
3.344SerGlu: 3.344 ± 0.065
2.758SerPhe: 2.758 ± 0.052
4.84SerGly: 4.84 ± 0.073
1.321SerHis: 1.321 ± 0.038
3.633SerIle: 3.633 ± 0.067
2.875SerLys: 2.875 ± 0.054
7.074SerLeu: 7.074 ± 0.096
1.487SerMet: 1.487 ± 0.044
2.479SerAsn: 2.479 ± 0.057
2.198SerPro: 2.198 ± 0.046
2.966SerGln: 2.966 ± 0.064
3.027SerArg: 3.027 ± 0.052
4.156SerSer: 4.156 ± 0.08
3.081SerThr: 3.081 ± 0.057
4.377SerVal: 4.377 ± 0.069
0.954SerTrp: 0.954 ± 0.029
1.991SerTyr: 1.991 ± 0.053
0.0SerXaa: 0.0 ± 0.0
Thr
5.615ThrAla: 5.615 ± 0.079
0.377ThrCys: 0.377 ± 0.021
3.113ThrAsp: 3.113 ± 0.056
3.443ThrGlu: 3.443 ± 0.066
1.69ThrPhe: 1.69 ± 0.041
3.855ThrGly: 3.855 ± 0.07
0.971ThrHis: 0.971 ± 0.031
2.872ThrIle: 2.872 ± 0.062
2.239ThrLys: 2.239 ± 0.051
6.514ThrLeu: 6.514 ± 0.088
1.031ThrMet: 1.031 ± 0.035
2.078ThrAsn: 2.078 ± 0.046
2.739ThrPro: 2.739 ± 0.062
2.406ThrGln: 2.406 ± 0.049
2.138ThrArg: 2.138 ± 0.054
3.143ThrSer: 3.143 ± 0.054
2.883ThrThr: 2.883 ± 0.063
3.692ThrVal: 3.692 ± 0.062
0.479ThrTrp: 0.479 ± 0.022
1.271ThrTyr: 1.271 ± 0.037
0.0ThrXaa: 0.0 ± 0.0
Val
6.842ValAla: 6.842 ± 0.1
0.69ValCys: 0.69 ± 0.028
3.559ValAsp: 3.559 ± 0.065
3.461ValGlu: 3.461 ± 0.062
2.807ValPhe: 2.807 ± 0.048
4.175ValGly: 4.175 ± 0.073
1.257ValHis: 1.257 ± 0.039
4.471ValIle: 4.471 ± 0.065
3.472ValLys: 3.472 ± 0.062
7.567ValLeu: 7.567 ± 0.095
1.794ValMet: 1.794 ± 0.042
2.947ValAsn: 2.947 ± 0.06
2.58ValPro: 2.58 ± 0.06
2.905ValGln: 2.905 ± 0.062
3.088ValArg: 3.088 ± 0.062
4.66ValSer: 4.66 ± 0.071
3.931ValThr: 3.931 ± 0.074
5.013ValVal: 5.013 ± 0.083
0.736ValTrp: 0.736 ± 0.029
1.779ValTyr: 1.779 ± 0.039
0.0ValXaa: 0.0 ± 0.0
Trp
0.832TrpAla: 0.832 ± 0.029
0.128TrpCys: 0.128 ± 0.011
0.517TrpAsp: 0.517 ± 0.026
0.339TrpGlu: 0.339 ± 0.02
0.633TrpPhe: 0.633 ± 0.028
0.592TrpGly: 0.592 ± 0.024
0.435TrpHis: 0.435 ± 0.022
0.534TrpIle: 0.534 ± 0.027
0.273TrpLys: 0.273 ± 0.017
2.161TrpLeu: 2.161 ± 0.06
0.275TrpMet: 0.275 ± 0.016
0.341TrpAsn: 0.341 ± 0.018
0.565TrpPro: 0.565 ± 0.029
1.643TrpGln: 1.643 ± 0.052
0.845TrpArg: 0.845 ± 0.033
0.632TrpSer: 0.632 ± 0.025
0.381TrpThr: 0.381 ± 0.019
0.731TrpVal: 0.731 ± 0.03
0.198TrpTrp: 0.198 ± 0.015
0.403TrpTyr: 0.403 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.575TyrAla: 2.575 ± 0.053
0.346TyrCys: 0.346 ± 0.02
1.478TyrAsp: 1.478 ± 0.042
1.272TyrGlu: 1.272 ± 0.04
1.333TyrPhe: 1.333 ± 0.042
2.093TyrGly: 2.093 ± 0.048
0.714TyrHis: 0.714 ± 0.029
1.763TyrIle: 1.763 ± 0.042
1.258TyrLys: 1.258 ± 0.037
3.544TyrLeu: 3.544 ± 0.065
0.59TyrMet: 0.59 ± 0.023
1.202TyrAsn: 1.202 ± 0.04
1.316TyrPro: 1.316 ± 0.035
2.346TyrGln: 2.346 ± 0.061
1.725TyrArg: 1.725 ± 0.046
2.052TyrSer: 2.052 ± 0.044
1.476TyrThr: 1.476 ± 0.033
1.746TyrVal: 1.746 ± 0.044
0.458TyrTrp: 0.458 ± 0.022
1.007TyrTyr: 1.007 ± 0.035
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2946 proteins (985838 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski