Synechococcus phage S-B43

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 262 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3M5D7|A0A3G3M5D7_9CAUD Uncharacterized protein OS=Synechococcus phage S-B43 OX=2484638 PE=4 SV=1
MM1 pKa = 7.7SNSTYY6 pKa = 10.88NGWSNYY12 pKa = 6.09EE13 pKa = 3.85TWNAALWIGNDD24 pKa = 2.88EE25 pKa = 4.19FLYY28 pKa = 9.44NTAKK32 pKa = 10.79ACVTYY37 pKa = 10.66RR38 pKa = 11.84EE39 pKa = 4.41GLEE42 pKa = 4.36SVWDD46 pKa = 4.07RR47 pKa = 11.84FILCMNNIGDD57 pKa = 4.07FTTGDD62 pKa = 3.55GVRR65 pKa = 11.84WDD67 pKa = 4.73DD68 pKa = 3.76PNIDD72 pKa = 3.73VNEE75 pKa = 4.1MNEE78 pKa = 4.19MLWEE82 pKa = 4.1LL83 pKa = 3.89

Molecular weight: 9.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514ABP9|A0A514ABP9_9CAUD Uncharacterized protein OS=Synechococcus phage S-B43 OX=2484638 PE=4 SV=1
MM1 pKa = 7.72SSFATSNLSKK11 pKa = 10.46IKK13 pKa = 10.25PKK15 pKa = 10.61LRR17 pKa = 11.84TTGRR21 pKa = 11.84VSGNFGKK28 pKa = 10.51NKK30 pKa = 9.5VRR32 pKa = 11.84TNGNNDD38 pKa = 3.08LGYY41 pKa = 9.51TSRR44 pKa = 11.84QSITPIAKK52 pKa = 10.1RR53 pKa = 11.84SDD55 pKa = 3.2YY56 pKa = 8.94CQRR59 pKa = 11.84MINLYY64 pKa = 8.83HH65 pKa = 5.55TTEE68 pKa = 4.8DD69 pKa = 3.44DD70 pKa = 3.17NMRR73 pKa = 11.84RR74 pKa = 11.84FAYY77 pKa = 9.62TEE79 pKa = 3.46LHH81 pKa = 6.14RR82 pKa = 11.84LNFFKK87 pKa = 10.94

Molecular weight: 10.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

262

0

262

65487

29

4337

250.0

27.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.804 ± 0.159

0.904 ± 0.096

6.563 ± 0.106

6.061 ± 0.242

4.486 ± 0.103

7.887 ± 0.229

1.434 ± 0.117

6.456 ± 0.102

5.626 ± 0.296

7.006 ± 0.121

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.907 ± 0.158

6.239 ± 0.186

3.926 ± 0.109

3.514 ± 0.083

3.975 ± 0.143

7.565 ± 0.236

7.809 ± 0.325

7.308 ± 0.198

1.044 ± 0.089

4.485 ± 0.11
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski