Amino acid dipepetide frequency for Synechococcus phage S-B43

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.848AlaAla: 3.848 ± 0.393
0.489AlaCys: 0.489 ± 0.09
3.543AlaAsp: 3.543 ± 0.248
3.222AlaGlu: 3.222 ± 0.299
2.214AlaPhe: 2.214 ± 0.193
5.207AlaGly: 5.207 ± 0.346
0.748AlaHis: 0.748 ± 0.129
3.115AlaIle: 3.115 ± 0.198
3.207AlaLys: 3.207 ± 0.304
3.909AlaLeu: 3.909 ± 0.277
1.13AlaMet: 1.13 ± 0.177
3.787AlaAsn: 3.787 ± 0.265
2.749AlaPro: 2.749 ± 0.183
2.153AlaGln: 2.153 ± 0.227
2.291AlaArg: 2.291 ± 0.201
4.78AlaSer: 4.78 ± 0.315
5.024AlaThr: 5.024 ± 0.324
3.65AlaVal: 3.65 ± 0.299
0.473AlaTrp: 0.473 ± 0.099
2.504AlaTyr: 2.504 ± 0.176
0.0AlaXaa: 0.0 ± 0.0
Cys
0.641CysAla: 0.641 ± 0.114
0.076CysCys: 0.076 ± 0.041
0.672CysAsp: 0.672 ± 0.126
0.657CysGlu: 0.657 ± 0.136
0.321CysPhe: 0.321 ± 0.067
0.657CysGly: 0.657 ± 0.111
0.199CysHis: 0.199 ± 0.054
0.534CysIle: 0.534 ± 0.096
0.534CysLys: 0.534 ± 0.106
0.519CysLeu: 0.519 ± 0.112
0.275CysMet: 0.275 ± 0.071
0.321CysAsn: 0.321 ± 0.085
0.382CysPro: 0.382 ± 0.09
0.351CysGln: 0.351 ± 0.075
0.473CysArg: 0.473 ± 0.094
0.702CysSer: 0.702 ± 0.124
0.473CysThr: 0.473 ± 0.081
0.718CysVal: 0.718 ± 0.116
0.122CysTrp: 0.122 ± 0.048
0.412CysTyr: 0.412 ± 0.084
0.0CysXaa: 0.0 ± 0.0
Asp
3.894AspAla: 3.894 ± 0.219
0.794AspCys: 0.794 ± 0.127
4.291AspAsp: 4.291 ± 0.264
3.619AspGlu: 3.619 ± 0.244
3.314AspPhe: 3.314 ± 0.218
5.757AspGly: 5.757 ± 0.293
1.054AspHis: 1.054 ± 0.141
4.169AspIle: 4.169 ± 0.204
3.482AspLys: 3.482 ± 0.208
5.07AspLeu: 5.07 ± 0.31
1.344AspMet: 1.344 ± 0.193
4.062AspAsn: 4.062 ± 0.241
2.84AspPro: 2.84 ± 0.189
1.771AspGln: 1.771 ± 0.152
2.504AspArg: 2.504 ± 0.21
4.062AspSer: 4.062 ± 0.292
4.367AspThr: 4.367 ± 0.275
4.78AspVal: 4.78 ± 0.382
0.916AspTrp: 0.916 ± 0.137
3.543AspTyr: 3.543 ± 0.242
0.0AspXaa: 0.0 ± 0.0
Glu
2.932GluAla: 2.932 ± 0.231
0.55GluCys: 0.55 ± 0.089
4.016GluAsp: 4.016 ± 0.29
5.024GluGlu: 5.024 ± 0.602
2.962GluPhe: 2.962 ± 0.222
4.215GluGly: 4.215 ± 0.303
0.779GluHis: 0.779 ± 0.12
4.184GluIle: 4.184 ± 0.258
3.512GluLys: 3.512 ± 0.298
4.932GluLeu: 4.932 ± 0.316
1.497GluMet: 1.497 ± 0.18
3.726GluAsn: 3.726 ± 0.219
1.71GluPro: 1.71 ± 0.148
2.245GluGln: 2.245 ± 0.255
2.718GluArg: 2.718 ± 0.231
3.573GluSer: 3.573 ± 0.244
3.359GluThr: 3.359 ± 0.201
5.161GluVal: 5.161 ± 0.259
0.702GluTrp: 0.702 ± 0.102
2.81GluTyr: 2.81 ± 0.194
0.0GluXaa: 0.0 ± 0.0
Phe
2.52PheAla: 2.52 ± 0.228
0.504PheCys: 0.504 ± 0.096
3.634PheAsp: 3.634 ± 0.238
2.84PheGlu: 2.84 ± 0.193
1.435PhePhe: 1.435 ± 0.165
3.115PheGly: 3.115 ± 0.228
0.641PheHis: 0.641 ± 0.112
2.627PheIle: 2.627 ± 0.184
3.024PheLys: 3.024 ± 0.223
3.069PheLeu: 3.069 ± 0.236
0.993PheMet: 0.993 ± 0.13
2.764PheAsn: 2.764 ± 0.218
1.542PhePro: 1.542 ± 0.168
1.527PheGln: 1.527 ± 0.171
1.863PheArg: 1.863 ± 0.165
3.527PheSer: 3.527 ± 0.263
3.619PheThr: 3.619 ± 0.185
3.146PheVal: 3.146 ± 0.277
0.504PheTrp: 0.504 ± 0.091
1.97PheTyr: 1.97 ± 0.165
0.0PheXaa: 0.0 ± 0.0
Gly
5.131GlyAla: 5.131 ± 0.319
0.626GlyCys: 0.626 ± 0.116
4.978GlyAsp: 4.978 ± 0.333
4.047GlyGlu: 4.047 ± 0.277
3.527GlyPhe: 3.527 ± 0.317
6.444GlyGly: 6.444 ± 0.436
0.977GlyHis: 0.977 ± 0.152
5.879GlyIle: 5.879 ± 0.426
3.512GlyLys: 3.512 ± 0.298
4.566GlyLeu: 4.566 ± 0.28
1.481GlyMet: 1.481 ± 0.159
4.81GlyAsn: 4.81 ± 0.3
2.016GlyPro: 2.016 ± 0.165
2.565GlyGln: 2.565 ± 0.178
3.115GlyArg: 3.115 ± 0.22
6.994GlySer: 6.994 ± 0.383
7.192GlyThr: 7.192 ± 0.522
6.291GlyVal: 6.291 ± 0.459
0.931GlyTrp: 0.931 ± 0.129
3.787GlyTyr: 3.787 ± 0.312
0.0GlyXaa: 0.0 ± 0.0
His
0.519HisAla: 0.519 ± 0.095
0.26HisCys: 0.26 ± 0.083
0.748HisAsp: 0.748 ± 0.132
0.84HisGlu: 0.84 ± 0.143
0.87HisPhe: 0.87 ± 0.116
1.038HisGly: 1.038 ± 0.126
0.321HisHis: 0.321 ± 0.061
1.161HisIle: 1.161 ± 0.175
0.931HisLys: 0.931 ± 0.123
1.038HisLeu: 1.038 ± 0.142
0.244HisMet: 0.244 ± 0.065
0.825HisAsn: 0.825 ± 0.124
0.733HisPro: 0.733 ± 0.117
0.504HisGln: 0.504 ± 0.077
0.779HisArg: 0.779 ± 0.145
0.962HisSer: 0.962 ± 0.104
0.993HisThr: 0.993 ± 0.133
0.718HisVal: 0.718 ± 0.105
0.153HisTrp: 0.153 ± 0.043
0.702HisTyr: 0.702 ± 0.127
0.0HisXaa: 0.0 ± 0.0
Ile
3.543IleAla: 3.543 ± 0.219
0.611IleCys: 0.611 ± 0.115
4.932IleAsp: 4.932 ± 0.277
4.627IleGlu: 4.627 ± 0.343
2.26IlePhe: 2.26 ± 0.185
4.887IleGly: 4.887 ± 0.265
0.901IleHis: 0.901 ± 0.112
3.558IleIle: 3.558 ± 0.273
4.154IleLys: 4.154 ± 0.3
4.428IleLeu: 4.428 ± 0.238
1.054IleMet: 1.054 ± 0.182
3.894IleAsn: 3.894 ± 0.268
2.886IlePro: 2.886 ± 0.218
2.214IleGln: 2.214 ± 0.23
2.764IleArg: 2.764 ± 0.219
4.887IleSer: 4.887 ± 0.3
5.406IleThr: 5.406 ± 0.344
4.413IleVal: 4.413 ± 0.426
0.641IleTrp: 0.641 ± 0.114
2.504IleTyr: 2.504 ± 0.199
0.0IleXaa: 0.0 ± 0.0
Lys
2.764LysAla: 2.764 ± 0.231
0.672LysCys: 0.672 ± 0.141
3.436LysAsp: 3.436 ± 0.299
3.558LysGlu: 3.558 ± 0.357
2.886LysPhe: 2.886 ± 0.208
3.298LysGly: 3.298 ± 0.274
0.916LysHis: 0.916 ± 0.124
4.031LysIle: 4.031 ± 0.288
4.383LysLys: 4.383 ± 0.396
4.657LysLeu: 4.657 ± 0.284
1.435LysMet: 1.435 ± 0.213
3.054LysAsn: 3.054 ± 0.224
2.123LysPro: 2.123 ± 0.207
1.832LysGln: 1.832 ± 0.172
2.336LysArg: 2.336 ± 0.22
3.68LysSer: 3.68 ± 0.283
3.726LysThr: 3.726 ± 0.296
4.123LysVal: 4.123 ± 0.285
0.55LysTrp: 0.55 ± 0.108
2.794LysTyr: 2.794 ± 0.221
0.0LysXaa: 0.0 ± 0.0
Leu
3.863LeuAla: 3.863 ± 0.263
0.626LeuCys: 0.626 ± 0.127
5.589LeuAsp: 5.589 ± 0.277
4.398LeuGlu: 4.398 ± 0.315
2.764LeuPhe: 2.764 ± 0.186
4.825LeuGly: 4.825 ± 0.282
1.267LeuHis: 1.267 ± 0.169
3.634LeuIle: 3.634 ± 0.237
4.26LeuLys: 4.26 ± 0.322
4.398LeuLeu: 4.398 ± 0.294
1.512LeuMet: 1.512 ± 0.205
4.841LeuAsn: 4.841 ± 0.297
2.978LeuPro: 2.978 ± 0.207
2.932LeuGln: 2.932 ± 0.217
3.192LeuArg: 3.192 ± 0.215
5.574LeuSer: 5.574 ± 0.348
5.497LeuThr: 5.497 ± 0.314
4.413LeuVal: 4.413 ± 0.242
0.565LeuTrp: 0.565 ± 0.099
2.932LeuTyr: 2.932 ± 0.188
0.0LeuXaa: 0.0 ± 0.0
Met
1.359MetAla: 1.359 ± 0.175
0.305MetCys: 0.305 ± 0.084
1.099MetAsp: 1.099 ± 0.148
1.115MetGlu: 1.115 ± 0.183
0.718MetPhe: 0.718 ± 0.127
1.008MetGly: 1.008 ± 0.129
0.183MetHis: 0.183 ± 0.064
1.39MetIle: 1.39 ± 0.165
1.527MetLys: 1.527 ± 0.205
1.13MetLeu: 1.13 ± 0.133
0.534MetMet: 0.534 ± 0.114
1.161MetAsn: 1.161 ± 0.149
0.916MetPro: 0.916 ± 0.117
0.596MetGln: 0.596 ± 0.096
1.038MetArg: 1.038 ± 0.15
1.176MetSer: 1.176 ± 0.161
1.649MetThr: 1.649 ± 0.181
1.161MetVal: 1.161 ± 0.153
0.214MetTrp: 0.214 ± 0.054
0.794MetTyr: 0.794 ± 0.129
0.0MetXaa: 0.0 ± 0.0
Asn
3.375AsnAla: 3.375 ± 0.219
0.489AsnCys: 0.489 ± 0.09
3.466AsnAsp: 3.466 ± 0.267
3.192AsnGlu: 3.192 ± 0.216
3.115AsnPhe: 3.115 ± 0.226
4.887AsnGly: 4.887 ± 0.331
1.191AsnHis: 1.191 ± 0.177
4.352AsnIle: 4.352 ± 0.401
3.283AsnLys: 3.283 ± 0.246
4.917AsnLeu: 4.917 ± 0.299
0.962AsnMet: 0.962 ± 0.135
3.833AsnAsn: 3.833 ± 0.301
3.344AsnPro: 3.344 ± 0.231
2.397AsnGln: 2.397 ± 0.192
1.985AsnArg: 1.985 ± 0.178
4.673AsnSer: 4.673 ± 0.401
4.749AsnThr: 4.749 ± 0.426
4.703AsnVal: 4.703 ± 0.309
0.657AsnTrp: 0.657 ± 0.129
2.825AsnTyr: 2.825 ± 0.201
0.0AsnXaa: 0.0 ± 0.0
Pro
2.229ProAla: 2.229 ± 0.218
0.26ProCys: 0.26 ± 0.066
3.054ProAsp: 3.054 ± 0.22
2.779ProGlu: 2.779 ± 0.233
1.802ProPhe: 1.802 ± 0.158
3.146ProGly: 3.146 ± 0.206
0.489ProHis: 0.489 ± 0.104
2.26ProIle: 2.26 ± 0.182
2.352ProLys: 2.352 ± 0.226
2.764ProLeu: 2.764 ± 0.231
0.443ProMet: 0.443 ± 0.091
2.123ProAsn: 2.123 ± 0.191
1.756ProPro: 1.756 ± 0.201
1.542ProGln: 1.542 ± 0.179
1.39ProArg: 1.39 ± 0.15
3.008ProSer: 3.008 ± 0.21
2.993ProThr: 2.993 ± 0.207
2.81ProVal: 2.81 ± 0.228
0.489ProTrp: 0.489 ± 0.097
1.573ProTyr: 1.573 ± 0.146
0.0ProXaa: 0.0 ± 0.0
Gln
1.512GlnAla: 1.512 ± 0.16
0.321GlnCys: 0.321 ± 0.066
1.909GlnAsp: 1.909 ± 0.152
2.397GlnGlu: 2.397 ± 0.203
1.771GlnPhe: 1.771 ± 0.144
2.336GlnGly: 2.336 ± 0.195
0.489GlnHis: 0.489 ± 0.112
2.611GlnIle: 2.611 ± 0.203
1.985GlnLys: 1.985 ± 0.208
2.993GlnLeu: 2.993 ± 0.191
0.825GlnMet: 0.825 ± 0.118
2.153GlnAsn: 2.153 ± 0.222
1.344GlnPro: 1.344 ± 0.141
1.283GlnGln: 1.283 ± 0.153
1.802GlnArg: 1.802 ± 0.162
2.657GlnSer: 2.657 ± 0.204
2.199GlnThr: 2.199 ± 0.144
2.459GlnVal: 2.459 ± 0.186
0.305GlnTrp: 0.305 ± 0.063
1.787GlnTyr: 1.787 ± 0.155
0.0GlnXaa: 0.0 ± 0.0
Arg
2.245ArgAla: 2.245 ± 0.178
0.428ArgCys: 0.428 ± 0.095
1.985ArgAsp: 1.985 ± 0.182
2.428ArgGlu: 2.428 ± 0.21
2.245ArgPhe: 2.245 ± 0.2
2.917ArgGly: 2.917 ± 0.213
0.611ArgHis: 0.611 ± 0.096
2.871ArgIle: 2.871 ± 0.174
2.611ArgLys: 2.611 ± 0.274
2.84ArgLeu: 2.84 ± 0.248
1.008ArgMet: 1.008 ± 0.116
2.947ArgAsn: 2.947 ± 0.204
1.267ArgPro: 1.267 ± 0.157
1.405ArgGln: 1.405 ± 0.159
1.726ArgArg: 1.726 ± 0.175
2.199ArgSer: 2.199 ± 0.174
2.046ArgThr: 2.046 ± 0.225
3.314ArgVal: 3.314 ± 0.385
0.473ArgTrp: 0.473 ± 0.089
2.184ArgTyr: 2.184 ± 0.222
0.0ArgXaa: 0.0 ± 0.0
Ser
5.131SerAla: 5.131 ± 0.271
0.428SerCys: 0.428 ± 0.085
4.505SerAsp: 4.505 ± 0.268
3.695SerGlu: 3.695 ± 0.224
3.405SerPhe: 3.405 ± 0.195
7.925SerGly: 7.925 ± 0.487
1.054SerHis: 1.054 ± 0.144
5.039SerIle: 5.039 ± 0.291
3.359SerLys: 3.359 ± 0.208
5.161SerLeu: 5.161 ± 0.366
1.069SerMet: 1.069 ± 0.142
5.207SerAsn: 5.207 ± 0.316
2.489SerPro: 2.489 ± 0.193
2.657SerGln: 2.657 ± 0.204
2.535SerArg: 2.535 ± 0.236
6.475SerSer: 6.475 ± 0.449
6.123SerThr: 6.123 ± 0.389
5.604SerVal: 5.604 ± 0.455
0.657SerTrp: 0.657 ± 0.111
3.13SerTyr: 3.13 ± 0.281
0.0SerXaa: 0.0 ± 0.0
Thr
5.161ThrAla: 5.161 ± 0.382
0.397ThrCys: 0.397 ± 0.094
4.26ThrAsp: 4.26 ± 0.277
3.894ThrGlu: 3.894 ± 0.247
4.031ThrPhe: 4.031 ± 0.318
7.589ThrGly: 7.589 ± 0.531
0.779ThrHis: 0.779 ± 0.105
5.787ThrIle: 5.787 ± 0.425
3.176ThrLys: 3.176 ± 0.23
5.314ThrLeu: 5.314 ± 0.37
0.947ThrMet: 0.947 ± 0.105
4.657ThrAsn: 4.657 ± 0.368
3.405ThrPro: 3.405 ± 0.219
2.52ThrGln: 2.52 ± 0.18
2.229ThrArg: 2.229 ± 0.185
6.414ThrSer: 6.414 ± 0.457
7.315ThrThr: 7.315 ± 0.574
6.23ThrVal: 6.23 ± 0.318
0.87ThrTrp: 0.87 ± 0.108
3.115ThrTyr: 3.115 ± 0.224
0.0ThrXaa: 0.0 ± 0.0
Val
4.276ValAla: 4.276 ± 0.266
0.519ValCys: 0.519 ± 0.107
5.1ValAsp: 5.1 ± 0.307
4.734ValGlu: 4.734 ± 0.259
2.718ValPhe: 2.718 ± 0.228
6.169ValGly: 6.169 ± 0.521
0.733ValHis: 0.733 ± 0.129
4.092ValIle: 4.092 ± 0.241
3.894ValLys: 3.894 ± 0.215
4.413ValLeu: 4.413 ± 0.287
1.176ValMet: 1.176 ± 0.164
4.398ValAsn: 4.398 ± 0.33
2.611ValPro: 2.611 ± 0.241
2.443ValGln: 2.443 ± 0.175
3.085ValArg: 3.085 ± 0.27
6.627ValSer: 6.627 ± 0.394
7.299ValThr: 7.299 ± 0.551
5.482ValVal: 5.482 ± 0.32
0.519ValTrp: 0.519 ± 0.085
2.779ValTyr: 2.779 ± 0.19
0.0ValXaa: 0.0 ± 0.0
Trp
0.596TrpAla: 0.596 ± 0.103
0.153TrpCys: 0.153 ± 0.041
0.977TrpAsp: 0.977 ± 0.131
0.702TrpGlu: 0.702 ± 0.127
0.366TrpPhe: 0.366 ± 0.084
0.672TrpGly: 0.672 ± 0.114
0.229TrpHis: 0.229 ± 0.056
0.702TrpIle: 0.702 ± 0.106
0.733TrpLys: 0.733 ± 0.139
0.641TrpLeu: 0.641 ± 0.1
0.305TrpMet: 0.305 ± 0.083
0.825TrpAsn: 0.825 ± 0.107
0.26TrpPro: 0.26 ± 0.066
0.321TrpGln: 0.321 ± 0.07
0.489TrpArg: 0.489 ± 0.102
0.596TrpSer: 0.596 ± 0.111
0.596TrpThr: 0.596 ± 0.091
0.596TrpVal: 0.596 ± 0.116
0.183TrpTrp: 0.183 ± 0.053
0.504TrpTyr: 0.504 ± 0.105
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.504TyrAla: 2.504 ± 0.193
0.534TyrCys: 0.534 ± 0.105
3.436TyrAsp: 3.436 ± 0.237
2.84TyrGlu: 2.84 ± 0.211
2.138TyrPhe: 2.138 ± 0.227
2.779TyrGly: 2.779 ± 0.248
0.779TyrHis: 0.779 ± 0.097
2.565TyrIle: 2.565 ± 0.215
2.336TyrLys: 2.336 ± 0.221
3.298TyrLeu: 3.298 ± 0.239
0.825TyrMet: 0.825 ± 0.12
3.008TyrAsn: 3.008 ± 0.209
1.909TyrPro: 1.909 ± 0.167
1.878TyrGln: 1.878 ± 0.203
1.435TyrArg: 1.435 ± 0.148
3.085TyrSer: 3.085 ± 0.18
3.466TyrThr: 3.466 ± 0.227
3.314TyrVal: 3.314 ± 0.222
0.519TyrTrp: 0.519 ± 0.106
2.199TyrTyr: 2.199 ± 0.177
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 262 proteins (65487 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski