Candidatus Syntrophocurvum alkaliphilum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Syntrophomonadaceae; Candidatus Syntrophocurvum

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2290 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I6D7F6|A0A6I6D7F6_9FIRM Glycosyl transferase group 2 family OS=Candidatus Syntrophocurvum alkaliphilum OX=2293317 GN=SYNTR_0004 PE=4 SV=1
MM1 pKa = 7.9KK2 pKa = 10.28YY3 pKa = 10.56GKK5 pKa = 9.52QGKK8 pKa = 9.18LFILFALIIFVNLIIIGCGPSDD30 pKa = 3.9DD31 pKa = 4.44PEE33 pKa = 4.64SVGEE37 pKa = 4.03VSVVDD42 pKa = 4.56LFNGQIIGIDD52 pKa = 3.45PGAGVMDD59 pKa = 4.01MAEE62 pKa = 4.14TALDD66 pKa = 3.75KK67 pKa = 11.59YY68 pKa = 11.1GLTDD72 pKa = 3.38AGYY75 pKa = 9.68EE76 pKa = 4.0LVEE79 pKa = 4.6GSDD82 pKa = 3.1ATMAAEE88 pKa = 4.82LDD90 pKa = 3.56RR91 pKa = 11.84AITNNEE97 pKa = 3.77WVVVTGWAPHH107 pKa = 5.26WKK109 pKa = 9.73FGKK112 pKa = 10.29YY113 pKa = 9.1DD114 pKa = 4.14LKK116 pKa = 11.04ILDD119 pKa = 4.69DD120 pKa = 4.11PLGVFGEE127 pKa = 4.56EE128 pKa = 3.58EE129 pKa = 4.35TINNIVRR136 pKa = 11.84LGLQDD141 pKa = 5.03DD142 pKa = 3.99MPEE145 pKa = 4.11VYY147 pKa = 10.21EE148 pKa = 5.19LLDD151 pKa = 3.26NHH153 pKa = 6.47FWTGEE158 pKa = 4.02DD159 pKa = 3.72LSAAMAIAEE168 pKa = 4.16EE169 pKa = 4.56SEE171 pKa = 4.37SAEE174 pKa = 3.71AAAAQWISEE183 pKa = 4.04NQVLVEE189 pKa = 3.96TWLPINFDD197 pKa = 3.62AEE199 pKa = 4.19ANAGQEE205 pKa = 4.02VTLLYY210 pKa = 10.78VEE212 pKa = 4.85WACASATTHH221 pKa = 6.87IIADD225 pKa = 3.37ILEE228 pKa = 4.65NIMGYY233 pKa = 8.25SVEE236 pKa = 4.02IMPVSASAMYY246 pKa = 9.45TGLATGVGDD255 pKa = 4.27AMVAAWLPSTHH266 pKa = 6.57SDD268 pKa = 3.38YY269 pKa = 10.86MKK271 pKa = 10.19QYY273 pKa = 9.78NYY275 pKa = 9.98QVEE278 pKa = 4.3DD279 pKa = 4.36LGPSMEE285 pKa = 4.13GAVLGLVVPEE295 pKa = 3.93YY296 pKa = 11.33VKK298 pKa = 10.23IDD300 pKa = 3.92SIEE303 pKa = 3.85EE304 pKa = 4.08MINN307 pKa = 3.08

Molecular weight: 33.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I6DIZ6|A0A6I6DIZ6_9FIRM CRISPR-associated endoribonuclease OS=Candidatus Syntrophocurvum alkaliphilum OX=2293317 GN=SYNTR_1211 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 9.51QPKK8 pKa = 8.32RR9 pKa = 11.84RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.28RR14 pKa = 11.84EE15 pKa = 3.54HH16 pKa = 6.3GFRR19 pKa = 11.84KK20 pKa = 9.99RR21 pKa = 11.84MSTQSGRR28 pKa = 11.84RR29 pKa = 11.84IIANRR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 10.04KK37 pKa = 10.26GRR39 pKa = 11.84KK40 pKa = 6.36TLSAA44 pKa = 4.15

Molecular weight: 5.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2290

0

2290

678767

29

2083

296.4

33.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.367 ± 0.051

0.988 ± 0.022

5.61 ± 0.046

7.63 ± 0.057

4.057 ± 0.037

6.514 ± 0.058

1.629 ± 0.022

9.343 ± 0.052

7.414 ± 0.053

9.568 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.454 ± 0.022

5.816 ± 0.048

3.42 ± 0.027

3.147 ± 0.025

3.895 ± 0.035

5.802 ± 0.036

5.13 ± 0.048

6.701 ± 0.044

0.796 ± 0.017

3.718 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski