Clostridium sp. MD294

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; unclassified Clostridium

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2792 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|N1ZF63|N1ZF63_9CLOT Uncharacterized protein OS=Clostridium sp. MD294 OX=97138 GN=C820_01881 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 10.42KK3 pKa = 9.98RR4 pKa = 11.84VLGLLLCSVMAFSMFAAGCGGSNNAPAPEE33 pKa = 3.91QTEE36 pKa = 4.15NGQNTSTSEE45 pKa = 4.03GGLKK49 pKa = 9.76IAIVSSPSGVDD60 pKa = 3.39DD61 pKa = 5.34GSFNQNNYY69 pKa = 10.33EE70 pKa = 4.37GIQSFIANHH79 pKa = 6.99PDD81 pKa = 3.21ASVTPVKK88 pKa = 10.6EE89 pKa = 4.07EE90 pKa = 4.16SGDD93 pKa = 3.34TAAAVQAVADD103 pKa = 3.93IVADD107 pKa = 3.66YY108 pKa = 11.18NVIVCCGFQFAGIGAITQDD127 pKa = 3.49NPNVNFILVDD137 pKa = 4.03SNPTDD142 pKa = 3.46AQGQEE147 pKa = 3.88INTEE151 pKa = 3.98NIYY154 pKa = 11.54AMTFAEE160 pKa = 4.3QEE162 pKa = 4.14SGFYY166 pKa = 10.56AGVAAALEE174 pKa = 4.35TQSNKK179 pKa = 9.18VAVVNGIAYY188 pKa = 7.57PSNVNYY194 pKa = 10.33QYY196 pKa = 11.48GFEE199 pKa = 4.28SGVNYY204 pKa = 10.62ANKK207 pKa = 10.24NLGTSAEE214 pKa = 4.24VVEE217 pKa = 4.34IASYY221 pKa = 11.2AGTDD225 pKa = 3.3VTGANVGGNYY235 pKa = 10.07VGTFADD241 pKa = 3.87EE242 pKa = 4.14ATGKK246 pKa = 10.26VVGNALINEE255 pKa = 4.55GCDD258 pKa = 3.16ILFVAAGGSGNGVFTAAKK276 pKa = 8.39EE277 pKa = 3.91ASNVKK282 pKa = 10.5VIGCDD287 pKa = 2.83VDD289 pKa = 4.4QYY291 pKa = 12.01DD292 pKa = 4.75DD293 pKa = 4.02GANGDD298 pKa = 4.13SNIVLTSVLKK308 pKa = 11.26VMDD311 pKa = 4.18KK312 pKa = 10.98NVEE315 pKa = 4.0KK316 pKa = 10.71QLNAVIDD323 pKa = 3.88GTFTGKK329 pKa = 10.1NDD331 pKa = 4.1LLGADD336 pKa = 4.16TDD338 pKa = 3.78STGYY342 pKa = 10.3VKK344 pKa = 10.65QEE346 pKa = 3.54GRR348 pKa = 11.84NQLSADD354 pKa = 4.21TITKK358 pKa = 9.99LDD360 pKa = 3.65EE361 pKa = 4.22VYY363 pKa = 10.97EE364 pKa = 4.03LVKK367 pKa = 11.06NGTVVPAANFNGILPEE383 pKa = 4.35DD384 pKa = 3.87LGG386 pKa = 3.98

Molecular weight: 39.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|N1ZMS6|N1ZMS6_9CLOT Uncharacterized protein OS=Clostridium sp. MD294 OX=97138 GN=C820_00687 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.07VHH16 pKa = 5.95GFRR19 pKa = 11.84KK20 pKa = 10.0RR21 pKa = 11.84MSTANGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2792

0

2792

835602

21

2163

299.3

33.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.598 ± 0.053

1.423 ± 0.024

4.972 ± 0.036

7.729 ± 0.054

4.224 ± 0.043

6.143 ± 0.05

1.585 ± 0.019

8.9 ± 0.051

8.42 ± 0.049

8.416 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.839 ± 0.023

5.44 ± 0.043

2.829 ± 0.026

4.372 ± 0.048

3.232 ± 0.03

5.667 ± 0.037

5.858 ± 0.054

6.342 ± 0.04

0.803 ± 0.015

4.208 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski