Amino acid dipepetide frequency for Clostridium sp. MD294

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.019AlaAla: 5.019 ± 0.116
0.853AlaCys: 0.853 ± 0.037
3.401AlaAsp: 3.401 ± 0.072
5.297AlaGlu: 5.297 ± 0.091
2.847AlaPhe: 2.847 ± 0.07
4.275AlaGly: 4.275 ± 0.086
0.851AlaHis: 0.851 ± 0.033
5.789AlaIle: 5.789 ± 0.096
5.36AlaLys: 5.36 ± 0.104
6.193AlaLeu: 6.193 ± 0.089
2.05AlaMet: 2.05 ± 0.063
2.878AlaAsn: 2.878 ± 0.073
1.692AlaPro: 1.692 ± 0.05
2.523AlaGln: 2.523 ± 0.08
1.942AlaArg: 1.942 ± 0.051
3.472AlaSer: 3.472 ± 0.071
3.564AlaThr: 3.564 ± 0.106
5.223AlaVal: 5.223 ± 0.105
0.443AlaTrp: 0.443 ± 0.023
2.309AlaTyr: 2.309 ± 0.056
0.0AlaXaa: 0.0 ± 0.0
Cys
0.854CysAla: 0.854 ± 0.036
0.378CysCys: 0.378 ± 0.021
0.752CysAsp: 0.752 ± 0.035
0.834CysGlu: 0.834 ± 0.031
0.765CysPhe: 0.765 ± 0.033
1.211CysGly: 1.211 ± 0.041
0.371CysHis: 0.371 ± 0.023
1.392CysIle: 1.392 ± 0.045
0.933CysLys: 0.933 ± 0.037
0.951CysLeu: 0.951 ± 0.032
0.379CysMet: 0.379 ± 0.021
0.762CysAsn: 0.762 ± 0.031
0.521CysPro: 0.521 ± 0.03
0.448CysGln: 0.448 ± 0.02
0.416CysArg: 0.416 ± 0.022
0.892CysSer: 0.892 ± 0.03
0.701CysThr: 0.701 ± 0.032
0.839CysVal: 0.839 ± 0.032
0.11CysTrp: 0.11 ± 0.012
0.722CysTyr: 0.722 ± 0.053
0.0CysXaa: 0.0 ± 0.0
Asp
3.126AspAla: 3.126 ± 0.076
0.67AspCys: 0.67 ± 0.028
2.492AspAsp: 2.492 ± 0.076
3.359AspGlu: 3.359 ± 0.079
2.735AspPhe: 2.735 ± 0.059
3.12AspGly: 3.12 ± 0.075
0.473AspHis: 0.473 ± 0.023
5.701AspIle: 5.701 ± 0.091
3.952AspLys: 3.952 ± 0.07
3.813AspLeu: 3.813 ± 0.071
1.819AspMet: 1.819 ± 0.045
2.556AspAsn: 2.556 ± 0.056
1.102AspPro: 1.102 ± 0.04
0.786AspGln: 0.786 ± 0.031
1.678AspArg: 1.678 ± 0.053
2.377AspSer: 2.377 ± 0.054
3.39AspThr: 3.39 ± 0.072
3.651AspVal: 3.651 ± 0.069
0.553AspTrp: 0.553 ± 0.029
2.368AspTyr: 2.368 ± 0.049
0.0AspXaa: 0.0 ± 0.0
Glu
4.604GluAla: 4.604 ± 0.079
0.795GluCys: 0.795 ± 0.038
3.433GluAsp: 3.433 ± 0.079
6.691GluGlu: 6.691 ± 0.122
2.529GluPhe: 2.529 ± 0.064
4.074GluGly: 4.074 ± 0.082
1.109GluHis: 1.109 ± 0.041
7.204GluIle: 7.204 ± 0.105
8.443GluLys: 8.443 ± 0.124
6.455GluLeu: 6.455 ± 0.096
2.481GluMet: 2.481 ± 0.062
5.632GluAsn: 5.632 ± 0.097
1.526GluPro: 1.526 ± 0.044
5.145GluGln: 5.145 ± 0.109
2.623GluArg: 2.623 ± 0.055
2.574GluSer: 2.574 ± 0.059
3.734GluThr: 3.734 ± 0.082
4.308GluVal: 4.308 ± 0.082
0.609GluTrp: 0.609 ± 0.04
3.319GluTyr: 3.319 ± 0.065
0.0GluXaa: 0.0 ± 0.0
Phe
2.7PheAla: 2.7 ± 0.061
0.817PheCys: 0.817 ± 0.033
2.565PheAsp: 2.565 ± 0.053
3.049PheGlu: 3.049 ± 0.064
2.057PhePhe: 2.057 ± 0.058
2.689PheGly: 2.689 ± 0.065
0.892PheHis: 0.892 ± 0.03
3.497PheIle: 3.497 ± 0.08
2.062PheLys: 2.062 ± 0.056
4.223PheLeu: 4.223 ± 0.092
1.112PheMet: 1.112 ± 0.037
1.497PheAsn: 1.497 ± 0.048
1.276PhePro: 1.276 ± 0.044
1.687PheGln: 1.687 ± 0.048
1.377PheArg: 1.377 ± 0.037
3.082PheSer: 3.082 ± 0.065
2.392PheThr: 2.392 ± 0.056
2.931PheVal: 2.931 ± 0.062
0.347PheTrp: 0.347 ± 0.019
1.989PheTyr: 1.989 ± 0.06
0.0PheXaa: 0.0 ± 0.0
Gly
4.264GlyAla: 4.264 ± 0.092
0.987GlyCys: 0.987 ± 0.04
2.966GlyAsp: 2.966 ± 0.064
4.47GlyGlu: 4.47 ± 0.081
2.709GlyPhe: 2.709 ± 0.057
4.225GlyGly: 4.225 ± 0.096
1.057GlyHis: 1.057 ± 0.042
6.158GlyIle: 6.158 ± 0.108
5.201GlyLys: 5.201 ± 0.078
4.463GlyLeu: 4.463 ± 0.09
1.92GlyMet: 1.92 ± 0.048
3.353GlyAsn: 3.353 ± 0.06
0.835GlyPro: 0.835 ± 0.065
1.893GlyGln: 1.893 ± 0.047
2.045GlyArg: 2.045 ± 0.057
3.36GlySer: 3.36 ± 0.066
3.901GlyThr: 3.901 ± 0.078
4.234GlyVal: 4.234 ± 0.097
0.527GlyTrp: 0.527 ± 0.025
2.86GlyTyr: 2.86 ± 0.059
0.0GlyXaa: 0.0 ± 0.0
His
0.801HisAla: 0.801 ± 0.03
0.381HisCys: 0.381 ± 0.024
0.676HisAsp: 0.676 ± 0.027
0.762HisGlu: 0.762 ± 0.035
0.916HisPhe: 0.916 ± 0.035
0.857HisGly: 0.857 ± 0.033
0.41HisHis: 0.41 ± 0.029
1.801HisIle: 1.801 ± 0.044
1.149HisLys: 1.149 ± 0.032
1.296HisLeu: 1.296 ± 0.037
0.501HisMet: 0.501 ± 0.024
0.936HisAsn: 0.936 ± 0.034
0.537HisPro: 0.537 ± 0.03
0.535HisGln: 0.535 ± 0.024
0.513HisArg: 0.513 ± 0.023
1.051HisSer: 1.051 ± 0.04
1.011HisThr: 1.011 ± 0.035
0.772HisVal: 0.772 ± 0.029
0.162HisTrp: 0.162 ± 0.016
0.78HisTyr: 0.78 ± 0.031
0.0HisXaa: 0.0 ± 0.0
Ile
6.722IleAla: 6.722 ± 0.106
1.476IleCys: 1.476 ± 0.045
4.897IleAsp: 4.897 ± 0.068
6.894IleGlu: 6.894 ± 0.096
3.443IlePhe: 3.443 ± 0.076
5.291IleGly: 5.291 ± 0.086
1.36IleHis: 1.36 ± 0.049
6.878IleIle: 6.878 ± 0.114
6.598IleLys: 6.598 ± 0.095
8.187IleLeu: 8.187 ± 0.128
2.195IleMet: 2.195 ± 0.05
4.123IleAsn: 4.123 ± 0.074
3.119IlePro: 3.119 ± 0.062
3.557IleGln: 3.557 ± 0.079
2.73IleArg: 2.73 ± 0.063
5.92IleSer: 5.92 ± 0.087
5.655IleThr: 5.655 ± 0.095
6.083IleVal: 6.083 ± 0.102
0.637IleTrp: 0.637 ± 0.029
3.237IleTyr: 3.237 ± 0.07
0.0IleXaa: 0.0 ± 0.0
Lys
5.309LysAla: 5.309 ± 0.099
0.862LysCys: 0.862 ± 0.035
3.879LysAsp: 3.879 ± 0.076
8.292LysGlu: 8.292 ± 0.129
2.203LysPhe: 2.203 ± 0.05
5.053LysGly: 5.053 ± 0.092
1.073LysHis: 1.073 ± 0.035
6.784LysIle: 6.784 ± 0.09
8.443LysLys: 8.443 ± 0.119
6.12LysLeu: 6.12 ± 0.095
2.565LysMet: 2.565 ± 0.057
5.562LysAsn: 5.562 ± 0.079
1.964LysPro: 1.964 ± 0.05
5.389LysGln: 5.389 ± 0.111
3.04LysArg: 3.04 ± 0.071
3.899LysSer: 3.899 ± 0.065
4.873LysThr: 4.873 ± 0.079
4.646LysVal: 4.646 ± 0.078
0.768LysTrp: 0.768 ± 0.03
3.474LysTyr: 3.474 ± 0.073
0.0LysXaa: 0.0 ± 0.0
Leu
5.079LeuAla: 5.079 ± 0.083
1.394LeuCys: 1.394 ± 0.049
3.983LeuAsp: 3.983 ± 0.085
6.851LeuGlu: 6.851 ± 0.103
4.295LeuPhe: 4.295 ± 0.097
4.928LeuGly: 4.928 ± 0.095
1.506LeuHis: 1.506 ± 0.042
5.759LeuIle: 5.759 ± 0.096
7.306LeuLys: 7.306 ± 0.104
8.426LeuLeu: 8.426 ± 0.134
1.994LeuMet: 1.994 ± 0.048
4.214LeuAsn: 4.214 ± 0.079
3.035LeuPro: 3.035 ± 0.065
3.974LeuGln: 3.974 ± 0.085
2.635LeuArg: 2.635 ± 0.056
6.078LeuSer: 6.078 ± 0.094
4.452LeuThr: 4.452 ± 0.075
4.16LeuVal: 4.16 ± 0.071
0.641LeuTrp: 0.641 ± 0.028
3.453LeuTyr: 3.453 ± 0.074
0.0LeuXaa: 0.0 ± 0.0
Met
2.025MetAla: 2.025 ± 0.05
0.302MetCys: 0.302 ± 0.018
1.376MetAsp: 1.376 ± 0.04
2.338MetGlu: 2.338 ± 0.05
0.951MetPhe: 0.951 ± 0.037
1.808MetGly: 1.808 ± 0.052
0.282MetHis: 0.282 ± 0.019
2.536MetIle: 2.536 ± 0.056
2.761MetLys: 2.761 ± 0.056
2.647MetLeu: 2.647 ± 0.06
0.741MetMet: 0.741 ± 0.032
1.496MetAsn: 1.496 ± 0.039
1.029MetPro: 1.029 ± 0.036
1.178MetGln: 1.178 ± 0.045
0.918MetArg: 0.918 ± 0.036
1.618MetSer: 1.618 ± 0.044
1.845MetThr: 1.845 ± 0.052
1.495MetVal: 1.495 ± 0.038
0.166MetTrp: 0.166 ± 0.013
0.876MetTyr: 0.876 ± 0.031
0.0MetXaa: 0.0 ± 0.0
Asn
3.426AsnAla: 3.426 ± 0.068
0.81AsnCys: 0.81 ± 0.036
2.792AsnAsp: 2.792 ± 0.06
3.657AsnGlu: 3.657 ± 0.08
2.076AsnPhe: 2.076 ± 0.06
3.804AsnGly: 3.804 ± 0.085
0.9AsnHis: 0.9 ± 0.03
6.076AsnIle: 6.076 ± 0.099
4.247AsnLys: 4.247 ± 0.075
3.742AsnLeu: 3.742 ± 0.06
1.623AsnMet: 1.623 ± 0.045
3.023AsnAsn: 3.023 ± 0.081
1.617AsnPro: 1.617 ± 0.043
1.839AsnGln: 1.839 ± 0.051
1.926AsnArg: 1.926 ± 0.049
2.908AsnSer: 2.908 ± 0.064
3.699AsnThr: 3.699 ± 0.094
3.466AsnVal: 3.466 ± 0.067
0.467AsnTrp: 0.467 ± 0.022
2.298AsnTyr: 2.298 ± 0.053
0.0AsnXaa: 0.0 ± 0.0
Pro
1.863ProAla: 1.863 ± 0.059
0.354ProCys: 0.354 ± 0.022
1.51ProAsp: 1.51 ± 0.048
2.557ProGlu: 2.557 ± 0.063
1.527ProPhe: 1.527 ± 0.041
1.275ProGly: 1.275 ± 0.047
0.512ProHis: 0.512 ± 0.023
2.433ProIle: 2.433 ± 0.056
2.113ProLys: 2.113 ± 0.052
2.362ProLeu: 2.362 ± 0.059
0.712ProMet: 0.712 ± 0.03
1.478ProAsn: 1.478 ± 0.045
0.786ProPro: 0.786 ± 0.037
1.2ProGln: 1.2 ± 0.039
0.708ProArg: 0.708 ± 0.027
1.809ProSer: 1.809 ± 0.042
1.577ProThr: 1.577 ± 0.059
2.066ProVal: 2.066 ± 0.036
0.174ProTrp: 0.174 ± 0.016
1.272ProTyr: 1.272 ± 0.039
0.0ProXaa: 0.0 ± 0.0
Gln
2.453GlnAla: 2.453 ± 0.057
0.54GlnCys: 0.54 ± 0.027
1.467GlnAsp: 1.467 ± 0.047
2.779GlnGlu: 2.779 ± 0.06
1.387GlnPhe: 1.387 ± 0.038
2.079GlnGly: 2.079 ± 0.061
0.657GlnHis: 0.657 ± 0.03
3.36GlnIle: 3.36 ± 0.08
5.93GlnLys: 5.93 ± 0.119
3.141GlnLeu: 3.141 ± 0.062
1.236GlnMet: 1.236 ± 0.039
3.382GlnAsn: 3.382 ± 0.092
1.066GlnPro: 1.066 ± 0.037
3.386GlnGln: 3.386 ± 0.115
1.531GlnArg: 1.531 ± 0.043
2.433GlnSer: 2.433 ± 0.058
2.532GlnThr: 2.532 ± 0.06
1.656GlnVal: 1.656 ± 0.046
0.436GlnTrp: 0.436 ± 0.024
2.271GlnTyr: 2.271 ± 0.071
0.0GlnXaa: 0.0 ± 0.0
Arg
1.99ArgAla: 1.99 ± 0.05
0.412ArgCys: 0.412 ± 0.024
1.421ArgAsp: 1.421 ± 0.042
2.634ArgGlu: 2.634 ± 0.072
1.395ArgPhe: 1.395 ± 0.041
1.863ArgGly: 1.863 ± 0.049
0.507ArgHis: 0.507 ± 0.022
2.859ArgIle: 2.859 ± 0.061
2.928ArgLys: 2.928 ± 0.06
2.7ArgLeu: 2.7 ± 0.063
1.05ArgMet: 1.05 ± 0.034
1.917ArgAsn: 1.917 ± 0.048
0.978ArgPro: 0.978 ± 0.036
1.443ArgGln: 1.443 ± 0.05
1.239ArgArg: 1.239 ± 0.046
1.549ArgSer: 1.549 ± 0.047
1.689ArgThr: 1.689 ± 0.045
2.038ArgVal: 2.038 ± 0.052
0.254ArgTrp: 0.254 ± 0.018
1.455ArgTyr: 1.455 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
3.755SerAla: 3.755 ± 0.075
0.722SerCys: 0.722 ± 0.033
2.98SerAsp: 2.98 ± 0.062
4.031SerGlu: 4.031 ± 0.073
2.652SerPhe: 2.652 ± 0.062
3.98SerGly: 3.98 ± 0.088
0.972SerHis: 0.972 ± 0.039
5.482SerIle: 5.482 ± 0.074
4.149SerLys: 4.149 ± 0.076
4.556SerLeu: 4.556 ± 0.091
1.528SerMet: 1.528 ± 0.046
2.951SerAsn: 2.951 ± 0.068
1.655SerPro: 1.655 ± 0.051
2.113SerGln: 2.113 ± 0.061
1.839SerArg: 1.839 ± 0.051
3.59SerSer: 3.59 ± 0.095
3.03SerThr: 3.03 ± 0.068
3.932SerVal: 3.932 ± 0.079
0.387SerTrp: 0.387 ± 0.018
2.36SerTyr: 2.36 ± 0.061
0.0SerXaa: 0.0 ± 0.0
Thr
4.684ThrAla: 4.684 ± 0.113
0.493ThrCys: 0.493 ± 0.027
2.949ThrAsp: 2.949 ± 0.075
4.71ThrGlu: 4.71 ± 0.093
2.451ThrPhe: 2.451 ± 0.053
3.961ThrGly: 3.961 ± 0.098
0.77ThrHis: 0.77 ± 0.027
5.44ThrIle: 5.44 ± 0.089
4.301ThrLys: 4.301 ± 0.083
5.055ThrLeu: 5.055 ± 0.084
1.537ThrMet: 1.537 ± 0.044
2.8ThrAsn: 2.8 ± 0.078
2.151ThrPro: 2.151 ± 0.057
2.24ThrGln: 2.24 ± 0.056
1.582ThrArg: 1.582 ± 0.043
3.061ThrSer: 3.061 ± 0.07
3.491ThrThr: 3.491 ± 0.088
4.557ThrVal: 4.557 ± 0.099
0.421ThrTrp: 0.421 ± 0.024
1.929ThrTyr: 1.929 ± 0.051
0.0ThrXaa: 0.0 ± 0.0
Val
4.398ValAla: 4.398 ± 0.105
0.997ValCys: 0.997 ± 0.039
3.165ValAsp: 3.165 ± 0.064
4.54ValGlu: 4.54 ± 0.09
2.767ValPhe: 2.767 ± 0.063
3.73ValGly: 3.73 ± 0.08
0.93ValHis: 0.93 ± 0.036
5.432ValIle: 5.432 ± 0.097
4.814ValLys: 4.814 ± 0.084
5.985ValLeu: 5.985 ± 0.089
1.796ValMet: 1.796 ± 0.043
3.013ValAsn: 3.013 ± 0.061
2.11ValPro: 2.11 ± 0.046
2.02ValGln: 2.02 ± 0.047
1.874ValArg: 1.874 ± 0.051
4.196ValSer: 4.196 ± 0.072
4.041ValThr: 4.041 ± 0.083
4.664ValVal: 4.664 ± 0.089
0.489ValTrp: 0.489 ± 0.025
2.457ValTyr: 2.457 ± 0.057
0.0ValXaa: 0.0 ± 0.0
Trp
0.488TrpAla: 0.488 ± 0.028
0.132TrpCys: 0.132 ± 0.014
0.428TrpAsp: 0.428 ± 0.026
0.621TrpGlu: 0.621 ± 0.029
0.371TrpPhe: 0.371 ± 0.024
0.551TrpGly: 0.551 ± 0.027
0.158TrpHis: 0.158 ± 0.016
0.649TrpIle: 0.649 ± 0.03
0.736TrpLys: 0.736 ± 0.031
0.725TrpLeu: 0.725 ± 0.034
0.136TrpMet: 0.136 ± 0.015
0.548TrpAsn: 0.548 ± 0.03
0.114TrpPro: 0.114 ± 0.013
0.431TrpGln: 0.431 ± 0.023
0.264TrpArg: 0.264 ± 0.017
0.395TrpSer: 0.395 ± 0.023
0.408TrpThr: 0.408 ± 0.023
0.364TrpVal: 0.364 ± 0.019
0.095TrpTrp: 0.095 ± 0.012
0.419TrpTyr: 0.419 ± 0.043
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.419TyrAla: 2.419 ± 0.051
0.858TyrCys: 0.858 ± 0.034
2.59TyrAsp: 2.59 ± 0.063
2.919TyrGlu: 2.919 ± 0.064
2.165TyrPhe: 2.165 ± 0.052
2.656TyrGly: 2.656 ± 0.061
1.058TyrHis: 1.058 ± 0.039
3.769TyrIle: 3.769 ± 0.077
2.77TyrLys: 2.77 ± 0.067
3.121TyrLeu: 3.121 ± 0.07
1.014TyrMet: 1.014 ± 0.04
2.274TyrAsn: 2.274 ± 0.064
1.179TyrPro: 1.179 ± 0.034
1.935TyrGln: 1.935 ± 0.049
1.44TyrArg: 1.44 ± 0.043
2.402TyrSer: 2.402 ± 0.064
2.597TyrThr: 2.597 ± 0.06
2.336TyrVal: 2.336 ± 0.056
0.348TyrTrp: 0.348 ± 0.021
2.23TyrTyr: 2.23 ± 0.077
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2792 proteins (835602 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski