Serratia phage PS2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Muldoonvirus; Serratia virus PS2

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 275 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A023W543|A0A023W543_9CAUD Uncharacterized protein OS=Serratia phage PS2 OX=1481112 GN=PS2_134 PE=4 SV=1
MM1 pKa = 7.83AIAICIKK8 pKa = 10.51SEE10 pKa = 3.94SCDD13 pKa = 3.5KK14 pKa = 11.11YY15 pKa = 10.76IYY17 pKa = 10.47AYY19 pKa = 8.84DD20 pKa = 3.35TGTPVEE26 pKa = 4.44TIKK29 pKa = 11.21EE30 pKa = 4.05EE31 pKa = 4.13LLGDD35 pKa = 3.63MDD37 pKa = 4.7MFAPICEE44 pKa = 4.26WEE46 pKa = 4.26YY47 pKa = 11.52SVSQSSMDD55 pKa = 3.49VNGDD59 pKa = 3.17IDD61 pKa = 4.49NMMSGIFDD69 pKa = 4.02ASWEE73 pKa = 4.21GRR75 pKa = 3.57

Molecular weight: 8.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A023W6M2|A0A023W6M2_9CAUD Dihydrofolate reductase OS=Serratia phage PS2 OX=1481112 GN=frd PE=3 SV=1
MM1 pKa = 7.13TMKK4 pKa = 10.33HH5 pKa = 6.03LPLIVSVLMATLTAAMLGITMTLGAVEE32 pKa = 5.3AISHH36 pKa = 5.32RR37 pKa = 11.84TFAEE41 pKa = 3.86LLGWSGLFVVIGLVASRR58 pKa = 11.84RR59 pKa = 11.84IKK61 pKa = 10.69

Molecular weight: 6.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

275

0

275

52757

20

1295

191.8

21.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.966 ± 0.172

1.069 ± 0.061

6.382 ± 0.108

7.102 ± 0.174

4.276 ± 0.115

6.265 ± 0.191

1.958 ± 0.075

6.689 ± 0.12

7.262 ± 0.183

7.561 ± 0.156

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.08 ± 0.093

5.029 ± 0.131

3.601 ± 0.098

3.694 ± 0.103

4.657 ± 0.099

6.409 ± 0.142

5.787 ± 0.179

6.795 ± 0.139

1.475 ± 0.062

3.943 ± 0.106
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski