Amino acid dipepetide frequency for Serratia phage PS2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.459AlaAla: 5.459 ± 0.376
0.682AlaCys: 0.682 ± 0.116
5.118AlaAsp: 5.118 ± 0.321
5.592AlaGlu: 5.592 ± 0.354
2.294AlaPhe: 2.294 ± 0.199
4.815AlaGly: 4.815 ± 0.418
1.308AlaHis: 1.308 ± 0.153
4.454AlaIle: 4.454 ± 0.322
4.739AlaLys: 4.739 ± 0.304
5.952AlaLeu: 5.952 ± 0.347
2.047AlaMet: 2.047 ± 0.23
3.279AlaAsn: 3.279 ± 0.29
2.275AlaPro: 2.275 ± 0.205
2.635AlaGln: 2.635 ± 0.213
3.052AlaArg: 3.052 ± 0.21
3.772AlaSer: 3.772 ± 0.317
4.094AlaThr: 4.094 ± 0.374
4.777AlaVal: 4.777 ± 0.307
1.118AlaTrp: 1.118 ± 0.142
2.199AlaTyr: 2.199 ± 0.207
0.0AlaXaa: 0.0 ± 0.0
Cys
0.796CysAla: 0.796 ± 0.15
0.095CysCys: 0.095 ± 0.044
0.682CysAsp: 0.682 ± 0.11
0.777CysGlu: 0.777 ± 0.134
0.474CysPhe: 0.474 ± 0.093
0.701CysGly: 0.701 ± 0.115
0.209CysHis: 0.209 ± 0.058
0.758CysIle: 0.758 ± 0.133
0.72CysLys: 0.72 ± 0.144
0.626CysLeu: 0.626 ± 0.101
0.436CysMet: 0.436 ± 0.099
0.284CysAsn: 0.284 ± 0.07
0.55CysPro: 0.55 ± 0.101
0.265CysGln: 0.265 ± 0.063
0.493CysArg: 0.493 ± 0.102
0.72CysSer: 0.72 ± 0.135
0.739CysThr: 0.739 ± 0.115
0.853CysVal: 0.853 ± 0.127
0.095CysTrp: 0.095 ± 0.045
0.417CysTyr: 0.417 ± 0.1
0.0CysXaa: 0.0 ± 0.0
Asp
4.947AspAla: 4.947 ± 0.299
0.55AspCys: 0.55 ± 0.099
3.791AspAsp: 3.791 ± 0.325
4.682AspGlu: 4.682 ± 0.35
3.355AspPhe: 3.355 ± 0.225
4.796AspGly: 4.796 ± 0.32
1.005AspHis: 1.005 ± 0.127
4.568AspIle: 4.568 ± 0.318
4.341AspLys: 4.341 ± 0.299
4.928AspLeu: 4.928 ± 0.322
1.839AspMet: 1.839 ± 0.181
2.369AspAsn: 2.369 ± 0.216
2.559AspPro: 2.559 ± 0.235
2.35AspGln: 2.35 ± 0.199
2.294AspArg: 2.294 ± 0.213
3.753AspSer: 3.753 ± 0.247
3.336AspThr: 3.336 ± 0.192
4.208AspVal: 4.208 ± 0.27
1.308AspTrp: 1.308 ± 0.165
2.843AspTyr: 2.843 ± 0.289
0.0AspXaa: 0.0 ± 0.0
Glu
4.53GluAla: 4.53 ± 0.345
0.986GluCys: 0.986 ± 0.138
4.132GluAsp: 4.132 ± 0.302
4.663GluGlu: 4.663 ± 0.396
3.393GluPhe: 3.393 ± 0.23
4.037GluGly: 4.037 ± 0.319
1.308GluHis: 1.308 ± 0.131
5.27GluIle: 5.27 ± 0.36
4.985GluLys: 4.985 ± 0.323
6.862GluLeu: 6.862 ± 0.483
2.369GluMet: 2.369 ± 0.197
2.976GluAsn: 2.976 ± 0.226
2.123GluPro: 2.123 ± 0.204
2.786GluGln: 2.786 ± 0.225
3.184GluArg: 3.184 ± 0.28
4.454GluSer: 4.454 ± 0.293
3.545GluThr: 3.545 ± 0.248
4.834GluVal: 4.834 ± 0.302
1.118GluTrp: 1.118 ± 0.136
3.469GluTyr: 3.469 ± 0.268
0.0GluXaa: 0.0 ± 0.0
Phe
3.071PheAla: 3.071 ± 0.247
0.474PheCys: 0.474 ± 0.105
3.052PheAsp: 3.052 ± 0.252
3.45PheGlu: 3.45 ± 0.294
1.308PhePhe: 1.308 ± 0.162
3.052PheGly: 3.052 ± 0.235
0.891PheHis: 0.891 ± 0.138
2.749PheIle: 2.749 ± 0.245
3.355PheLys: 3.355 ± 0.267
2.483PheLeu: 2.483 ± 0.231
1.289PheMet: 1.289 ± 0.181
2.673PheAsn: 2.673 ± 0.227
1.099PhePro: 1.099 ± 0.146
1.422PheGln: 1.422 ± 0.163
2.009PheArg: 2.009 ± 0.197
2.749PheSer: 2.749 ± 0.24
2.502PheThr: 2.502 ± 0.214
2.692PheVal: 2.692 ± 0.239
0.588PheTrp: 0.588 ± 0.121
1.858PheTyr: 1.858 ± 0.176
0.0PheXaa: 0.0 ± 0.0
Gly
3.696GlyAla: 3.696 ± 0.344
0.701GlyCys: 0.701 ± 0.101
4.037GlyAsp: 4.037 ± 0.305
3.791GlyGlu: 3.791 ± 0.233
2.805GlyPhe: 2.805 ± 0.299
3.393GlyGly: 3.393 ± 0.441
1.043GlyHis: 1.043 ± 0.16
4.663GlyIle: 4.663 ± 0.288
5.137GlyLys: 5.137 ± 0.365
4.777GlyLeu: 4.777 ± 0.351
2.085GlyMet: 2.085 ± 0.201
3.071GlyAsn: 3.071 ± 0.291
1.706GlyPro: 1.706 ± 0.172
2.464GlyGln: 2.464 ± 0.255
3.147GlyArg: 3.147 ± 0.237
4.303GlySer: 4.303 ± 0.377
4.151GlyThr: 4.151 ± 0.378
3.734GlyVal: 3.734 ± 0.307
1.194GlyTrp: 1.194 ± 0.152
2.749GlyTyr: 2.749 ± 0.23
0.0GlyXaa: 0.0 ± 0.0
His
1.213HisAla: 1.213 ± 0.139
0.171HisCys: 0.171 ± 0.061
1.403HisAsp: 1.403 ± 0.149
1.573HisGlu: 1.573 ± 0.184
1.043HisPhe: 1.043 ± 0.131
1.137HisGly: 1.137 ± 0.163
0.417HisHis: 0.417 ± 0.078
1.213HisIle: 1.213 ± 0.175
1.479HisLys: 1.479 ± 0.185
1.63HisLeu: 1.63 ± 0.176
0.569HisMet: 0.569 ± 0.106
0.948HisAsn: 0.948 ± 0.142
0.986HisPro: 0.986 ± 0.159
0.588HisGln: 0.588 ± 0.104
0.853HisArg: 0.853 ± 0.135
1.156HisSer: 1.156 ± 0.13
0.929HisThr: 0.929 ± 0.119
1.27HisVal: 1.27 ± 0.151
0.341HisTrp: 0.341 ± 0.065
0.663HisTyr: 0.663 ± 0.09
0.0HisXaa: 0.0 ± 0.0
Ile
4.644IleAla: 4.644 ± 0.326
0.796IleCys: 0.796 ± 0.109
4.758IleAsp: 4.758 ± 0.323
5.023IleGlu: 5.023 ± 0.361
2.54IlePhe: 2.54 ± 0.229
3.374IleGly: 3.374 ± 0.269
1.46IleHis: 1.46 ± 0.165
3.658IleIle: 3.658 ± 0.277
5.023IleLys: 5.023 ± 0.306
4.019IleLeu: 4.019 ± 0.284
2.047IleMet: 2.047 ± 0.208
3.905IleAsn: 3.905 ± 0.272
2.767IlePro: 2.767 ± 0.208
2.559IleGln: 2.559 ± 0.224
3.62IleArg: 3.62 ± 0.311
4.265IleSer: 4.265 ± 0.304
4.473IleThr: 4.473 ± 0.343
5.004IleVal: 5.004 ± 0.293
0.758IleTrp: 0.758 ± 0.123
2.199IleTyr: 2.199 ± 0.2
0.0IleXaa: 0.0 ± 0.0
Lys
5.27LysAla: 5.27 ± 0.298
0.626LysCys: 0.626 ± 0.11
4.36LysAsp: 4.36 ± 0.275
5.175LysGlu: 5.175 ± 0.377
3.355LysPhe: 3.355 ± 0.268
4.132LysGly: 4.132 ± 0.258
1.535LysHis: 1.535 ± 0.18
5.061LysIle: 5.061 ± 0.271
4.511LysLys: 4.511 ± 0.284
6.16LysLeu: 6.16 ± 0.387
2.862LysMet: 2.862 ± 0.246
3.526LysAsn: 3.526 ± 0.287
2.559LysPro: 2.559 ± 0.265
2.464LysGln: 2.464 ± 0.239
3.203LysArg: 3.203 ± 0.277
5.004LysSer: 5.004 ± 0.319
4.019LysThr: 4.019 ± 0.337
4.53LysVal: 4.53 ± 0.281
1.118LysTrp: 1.118 ± 0.18
3.147LysTyr: 3.147 ± 0.257
0.0LysXaa: 0.0 ± 0.0
Leu
4.909LeuAla: 4.909 ± 0.33
0.834LeuCys: 0.834 ± 0.117
4.815LeuAsp: 4.815 ± 0.3
5.497LeuGlu: 5.497 ± 0.317
2.919LeuPhe: 2.919 ± 0.232
4.341LeuGly: 4.341 ± 0.337
1.668LeuHis: 1.668 ± 0.143
4.341LeuIle: 4.341 ± 0.286
6.388LeuLys: 6.388 ± 0.341
4.853LeuLeu: 4.853 ± 0.298
2.711LeuMet: 2.711 ± 0.224
4.568LeuAsn: 4.568 ± 0.313
3.412LeuPro: 3.412 ± 0.272
2.881LeuGln: 2.881 ± 0.195
3.62LeuArg: 3.62 ± 0.285
4.682LeuSer: 4.682 ± 0.352
4.758LeuThr: 4.758 ± 0.339
5.08LeuVal: 5.08 ± 0.296
0.682LeuTrp: 0.682 ± 0.11
2.654LeuTyr: 2.654 ± 0.214
0.0LeuXaa: 0.0 ± 0.0
Met
2.426MetAla: 2.426 ± 0.197
0.265MetCys: 0.265 ± 0.068
2.009MetAsp: 2.009 ± 0.206
1.592MetGlu: 1.592 ± 0.175
1.308MetPhe: 1.308 ± 0.173
1.27MetGly: 1.27 ± 0.154
0.815MetHis: 0.815 ± 0.136
2.028MetIle: 2.028 ± 0.186
3.45MetLys: 3.45 ± 0.25
2.275MetLeu: 2.275 ± 0.215
0.986MetMet: 0.986 ± 0.138
1.914MetAsn: 1.914 ± 0.171
1.232MetPro: 1.232 ± 0.153
1.08MetGln: 1.08 ± 0.179
1.327MetArg: 1.327 ± 0.163
2.199MetSer: 2.199 ± 0.174
2.009MetThr: 2.009 ± 0.196
1.479MetVal: 1.479 ± 0.17
0.322MetTrp: 0.322 ± 0.084
0.815MetTyr: 0.815 ± 0.127
0.0MetXaa: 0.0 ± 0.0
Asn
3.431AsnAla: 3.431 ± 0.282
0.626AsnCys: 0.626 ± 0.106
3.071AsnAsp: 3.071 ± 0.239
3.26AsnGlu: 3.26 ± 0.279
2.256AsnPhe: 2.256 ± 0.204
3.791AsnGly: 3.791 ± 0.275
1.005AsnHis: 1.005 ± 0.155
3.109AsnIle: 3.109 ± 0.215
2.957AsnLys: 2.957 ± 0.19
4.094AsnLeu: 4.094 ± 0.263
1.384AsnMet: 1.384 ± 0.177
2.749AsnAsn: 2.749 ± 0.218
2.313AsnPro: 2.313 ± 0.218
1.725AsnGln: 1.725 ± 0.196
2.426AsnArg: 2.426 ± 0.24
3.298AsnSer: 3.298 ± 0.196
2.597AsnThr: 2.597 ± 0.278
3.431AsnVal: 3.431 ± 0.265
0.644AsnTrp: 0.644 ± 0.122
2.123AsnTyr: 2.123 ± 0.206
0.0AsnXaa: 0.0 ± 0.0
Pro
2.54ProAla: 2.54 ± 0.26
0.36ProCys: 0.36 ± 0.097
2.483ProAsp: 2.483 ± 0.218
3.128ProGlu: 3.128 ± 0.221
1.46ProPhe: 1.46 ± 0.162
2.805ProGly: 2.805 ± 0.25
0.834ProHis: 0.834 ± 0.124
1.971ProIle: 1.971 ± 0.191
2.331ProLys: 2.331 ± 0.255
2.218ProLeu: 2.218 ± 0.224
0.796ProMet: 0.796 ± 0.117
2.142ProAsn: 2.142 ± 0.237
0.72ProPro: 0.72 ± 0.133
1.27ProGln: 1.27 ± 0.149
1.687ProArg: 1.687 ± 0.178
2.483ProSer: 2.483 ± 0.212
1.896ProThr: 1.896 ± 0.176
2.711ProVal: 2.711 ± 0.264
0.588ProTrp: 0.588 ± 0.113
1.592ProTyr: 1.592 ± 0.185
0.0ProXaa: 0.0 ± 0.0
Gln
2.502GlnAla: 2.502 ± 0.207
0.322GlnCys: 0.322 ± 0.081
2.066GlnAsp: 2.066 ± 0.228
2.237GlnGlu: 2.237 ± 0.219
2.104GlnPhe: 2.104 ± 0.218
2.237GlnGly: 2.237 ± 0.213
0.758GlnHis: 0.758 ± 0.11
2.843GlnIle: 2.843 ± 0.224
2.692GlnLys: 2.692 ± 0.228
2.957GlnLeu: 2.957 ± 0.248
1.118GlnMet: 1.118 ± 0.168
1.687GlnAsn: 1.687 ± 0.225
1.346GlnPro: 1.346 ± 0.159
1.175GlnGln: 1.175 ± 0.178
1.858GlnArg: 1.858 ± 0.176
2.331GlnSer: 2.331 ± 0.234
1.763GlnThr: 1.763 ± 0.191
2.654GlnVal: 2.654 ± 0.18
0.663GlnTrp: 0.663 ± 0.116
1.63GlnTyr: 1.63 ± 0.172
0.0GlnXaa: 0.0 ± 0.0
Arg
3.317ArgAla: 3.317 ± 0.276
0.455ArgCys: 0.455 ± 0.096
2.749ArgAsp: 2.749 ± 0.227
3.222ArgGlu: 3.222 ± 0.305
1.933ArgPhe: 1.933 ± 0.177
2.616ArgGly: 2.616 ± 0.237
0.948ArgHis: 0.948 ± 0.141
3.355ArgIle: 3.355 ± 0.262
3.355ArgLys: 3.355 ± 0.29
3.564ArgLeu: 3.564 ± 0.302
1.194ArgMet: 1.194 ± 0.165
2.521ArgAsn: 2.521 ± 0.189
1.441ArgPro: 1.441 ± 0.154
2.18ArgGln: 2.18 ± 0.199
2.331ArgArg: 2.331 ± 0.223
2.957ArgSer: 2.957 ± 0.258
2.749ArgThr: 2.749 ± 0.244
3.374ArgVal: 3.374 ± 0.247
0.682ArgTrp: 0.682 ± 0.119
1.63ArgTyr: 1.63 ± 0.17
0.0ArgXaa: 0.0 ± 0.0
Ser
4.398SerAla: 4.398 ± 0.314
0.682SerCys: 0.682 ± 0.117
4.151SerAsp: 4.151 ± 0.34
4.189SerGlu: 4.189 ± 0.292
2.73SerPhe: 2.73 ± 0.187
4.853SerGly: 4.853 ± 0.335
1.024SerHis: 1.024 ± 0.132
4.568SerIle: 4.568 ± 0.313
4.151SerLys: 4.151 ± 0.257
4.625SerLeu: 4.625 ± 0.284
1.99SerMet: 1.99 ± 0.184
2.73SerAsn: 2.73 ± 0.237
2.142SerPro: 2.142 ± 0.185
2.313SerGln: 2.313 ± 0.179
3.147SerArg: 3.147 ± 0.269
4.796SerSer: 4.796 ± 0.366
3.772SerThr: 3.772 ± 0.298
4.303SerVal: 4.303 ± 0.339
1.043SerTrp: 1.043 ± 0.16
2.483SerTyr: 2.483 ± 0.231
0.0SerXaa: 0.0 ± 0.0
Thr
4.682ThrAla: 4.682 ± 0.393
0.474ThrCys: 0.474 ± 0.092
2.938ThrAsp: 2.938 ± 0.297
3.848ThrGlu: 3.848 ± 0.271
2.407ThrPhe: 2.407 ± 0.203
4.113ThrGly: 4.113 ± 0.332
0.967ThrHis: 0.967 ± 0.136
4.132ThrIle: 4.132 ± 0.3
4.151ThrLys: 4.151 ± 0.294
4.966ThrLeu: 4.966 ± 0.278
1.213ThrMet: 1.213 ± 0.145
2.616ThrAsn: 2.616 ± 0.196
2.559ThrPro: 2.559 ± 0.209
1.896ThrGln: 1.896 ± 0.215
2.711ThrArg: 2.711 ± 0.196
3.241ThrSer: 3.241 ± 0.254
3.374ThrThr: 3.374 ± 0.262
4.606ThrVal: 4.606 ± 0.404
0.72ThrTrp: 0.72 ± 0.114
2.256ThrTyr: 2.256 ± 0.189
0.0ThrXaa: 0.0 ± 0.0
Val
4.36ValAla: 4.36 ± 0.327
0.815ValCys: 0.815 ± 0.125
4.0ValAsp: 4.0 ± 0.271
5.44ValGlu: 5.44 ± 0.31
2.54ValPhe: 2.54 ± 0.18
3.829ValGly: 3.829 ± 0.267
1.441ValHis: 1.441 ± 0.164
4.739ValIle: 4.739 ± 0.295
5.175ValLys: 5.175 ± 0.361
4.966ValLeu: 4.966 ± 0.296
2.161ValMet: 2.161 ± 0.232
3.488ValAsn: 3.488 ± 0.241
2.445ValPro: 2.445 ± 0.253
2.767ValGln: 2.767 ± 0.23
3.222ValArg: 3.222 ± 0.245
4.151ValSer: 4.151 ± 0.311
4.151ValThr: 4.151 ± 0.408
4.739ValVal: 4.739 ± 0.362
1.005ValTrp: 1.005 ± 0.116
2.502ValTyr: 2.502 ± 0.192
0.0ValXaa: 0.0 ± 0.0
Trp
0.891TrpAla: 0.891 ± 0.12
0.152TrpCys: 0.152 ± 0.053
1.043TrpAsp: 1.043 ± 0.142
1.194TrpGlu: 1.194 ± 0.159
0.758TrpPhe: 0.758 ± 0.118
0.663TrpGly: 0.663 ± 0.125
0.171TrpHis: 0.171 ± 0.057
0.91TrpIle: 0.91 ± 0.141
1.08TrpLys: 1.08 ± 0.146
0.91TrpLeu: 0.91 ± 0.136
0.663TrpMet: 0.663 ± 0.111
0.872TrpAsn: 0.872 ± 0.137
0.379TrpPro: 0.379 ± 0.079
0.569TrpGln: 0.569 ± 0.093
0.569TrpArg: 0.569 ± 0.122
1.08TrpSer: 1.08 ± 0.134
0.815TrpThr: 0.815 ± 0.124
1.137TrpVal: 1.137 ± 0.142
0.209TrpTrp: 0.209 ± 0.061
0.682TrpTyr: 0.682 ± 0.099
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.578TyrAla: 2.578 ± 0.212
0.626TyrCys: 0.626 ± 0.108
3.166TyrAsp: 3.166 ± 0.262
2.692TyrGlu: 2.692 ± 0.221
1.782TyrPhe: 1.782 ± 0.203
2.692TyrGly: 2.692 ± 0.228
0.777TyrHis: 0.777 ± 0.136
2.502TyrIle: 2.502 ± 0.212
2.597TyrLys: 2.597 ± 0.199
2.749TyrLeu: 2.749 ± 0.25
1.024TyrMet: 1.024 ± 0.136
1.971TyrAsn: 1.971 ± 0.191
1.403TyrPro: 1.403 ± 0.172
1.554TyrGln: 1.554 ± 0.169
1.82TyrArg: 1.82 ± 0.19
2.692TyrSer: 2.692 ± 0.232
2.199TyrThr: 2.199 ± 0.207
2.54TyrVal: 2.54 ± 0.237
0.55TyrTrp: 0.55 ± 0.106
1.516TyrTyr: 1.516 ± 0.221
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 275 proteins (52757 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski