Lactobacillus frumenti DSM 13145

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Limosilactobacillus; Limosilactobacillus frumenti

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1641 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R1PDR8|A0A0R1PDR8_9LACO HxlR family transcriptional regulator OS=Lactobacillus frumenti DSM 13145 OX=1423746 GN=FD27_GL000527 PE=4 SV=1
MM1 pKa = 7.66AAQIYY6 pKa = 9.16PYY8 pKa = 9.98LTFEE12 pKa = 4.17NAKK15 pKa = 10.15EE16 pKa = 3.85AMDD19 pKa = 4.03YY20 pKa = 10.69YY21 pKa = 10.55VQNFGATILYY31 pKa = 7.49HH32 pKa = 6.4QPLSQQQAEE41 pKa = 4.23DD42 pKa = 3.62LGLNLDD48 pKa = 4.06NLANTIFRR56 pKa = 11.84GAFSIAGQKK65 pKa = 9.45LICADD70 pKa = 3.23ATMASPQASSLISIMLDD87 pKa = 3.77FYY89 pKa = 11.54DD90 pKa = 5.61DD91 pKa = 3.5EE92 pKa = 7.05GEE94 pKa = 4.05AKK96 pKa = 10.36EE97 pKa = 4.06LFKK100 pKa = 11.22LLSSSDD106 pKa = 3.71EE107 pKa = 3.89QRR109 pKa = 11.84VTIPFGPQNGGQMGQVIDD127 pKa = 4.23KK128 pKa = 11.18YY129 pKa = 10.28GITWIIVTGSLEE141 pKa = 4.01DD142 pKa = 3.35

Molecular weight: 15.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R1PJB3|A0A0R1PJB3_9LACO Sortase OS=Lactobacillus frumenti DSM 13145 OX=1423746 GN=FD27_GL001525 PE=4 SV=1
MM1 pKa = 7.44FLRR4 pKa = 11.84AKK6 pKa = 9.74GLSIRR11 pKa = 11.84AVALWLGRR19 pKa = 11.84NPSTISRR26 pKa = 11.84EE27 pKa = 4.31LKK29 pKa = 9.96RR30 pKa = 11.84CAGNYY35 pKa = 9.8SPSKK39 pKa = 10.64ADD41 pKa = 3.19NDD43 pKa = 3.89YY44 pKa = 10.51HH45 pKa = 7.29QKK47 pKa = 10.27RR48 pKa = 11.84QNCHH52 pKa = 5.5KK53 pKa = 10.55KK54 pKa = 10.26RR55 pKa = 11.84LLDD58 pKa = 3.24SHH60 pKa = 6.44PQLRR64 pKa = 11.84RR65 pKa = 11.84QIVHH69 pKa = 6.68YY70 pKa = 10.39ILDD73 pKa = 3.98LHH75 pKa = 6.81WSPEE79 pKa = 4.06QITARR84 pKa = 11.84FNKK87 pKa = 7.91EE88 pKa = 3.86HH89 pKa = 5.62QWCVSYY95 pKa = 9.22NTIYY99 pKa = 10.55RR100 pKa = 11.84HH101 pKa = 6.45IYY103 pKa = 7.76QHH105 pKa = 6.4NLGEE109 pKa = 4.71KK110 pKa = 8.59YY111 pKa = 10.24SSRR114 pKa = 11.84GDD116 pKa = 3.37TGIQRR121 pKa = 11.84HH122 pKa = 5.81LRR124 pKa = 11.84HH125 pKa = 5.83KK126 pKa = 10.42HH127 pKa = 3.72RR128 pKa = 11.84TRR130 pKa = 11.84HH131 pKa = 4.86SKK133 pKa = 7.37NTRR136 pKa = 11.84RR137 pKa = 11.84HH138 pKa = 5.84RR139 pKa = 11.84EE140 pKa = 3.68VQTDD144 pKa = 3.66YY145 pKa = 11.39ISIHH149 pKa = 5.77EE150 pKa = 4.45RR151 pKa = 11.84PGFINQRR158 pKa = 3.41

Molecular weight: 19.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1641

0

1641

504748

39

4898

307.6

34.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.935 ± 0.062

0.585 ± 0.019

6.029 ± 0.068

4.916 ± 0.056

4.084 ± 0.051

6.725 ± 0.057

2.84 ± 0.031

6.865 ± 0.066

6.101 ± 0.061

9.452 ± 0.086

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.815 ± 0.039

4.987 ± 0.073

3.815 ± 0.046

5.106 ± 0.063

4.367 ± 0.054

5.664 ± 0.051

5.839 ± 0.071

7.094 ± 0.049

1.16 ± 0.03

3.622 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski