Amino acid dipepetide frequency for Lactobacillus frumenti DSM 13145

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.94AlaAla: 6.94 ± 0.158
0.515AlaCys: 0.515 ± 0.033
5.183AlaAsp: 5.183 ± 0.131
3.964AlaGlu: 3.964 ± 0.079
3.128AlaPhe: 3.128 ± 0.093
5.942AlaGly: 5.942 ± 0.108
1.975AlaHis: 1.975 ± 0.071
5.734AlaIle: 5.734 ± 0.115
5.375AlaLys: 5.375 ± 0.11
7.342AlaLeu: 7.342 ± 0.153
2.144AlaMet: 2.144 ± 0.068
4.022AlaAsn: 4.022 ± 0.1
2.371AlaPro: 2.371 ± 0.068
3.665AlaGln: 3.665 ± 0.096
3.099AlaArg: 3.099 ± 0.074
4.222AlaSer: 4.222 ± 0.093
4.707AlaThr: 4.707 ± 0.12
5.803AlaVal: 5.803 ± 0.116
0.751AlaTrp: 0.751 ± 0.048
2.471AlaTyr: 2.471 ± 0.074
0.0AlaXaa: 0.0 ± 0.0
Cys
0.442CysAla: 0.442 ± 0.033
0.067CysCys: 0.067 ± 0.012
0.329CysAsp: 0.329 ± 0.028
0.226CysGlu: 0.226 ± 0.019
0.289CysPhe: 0.289 ± 0.024
0.586CysGly: 0.586 ± 0.038
0.254CysHis: 0.254 ± 0.027
0.329CysIle: 0.329 ± 0.027
0.168CysLys: 0.168 ± 0.02
0.668CysLeu: 0.668 ± 0.037
0.117CysMet: 0.117 ± 0.013
0.186CysAsn: 0.186 ± 0.017
0.293CysPro: 0.293 ± 0.028
0.299CysGln: 0.299 ± 0.025
0.262CysArg: 0.262 ± 0.027
0.337CysSer: 0.337 ± 0.025
0.283CysThr: 0.283 ± 0.025
0.367CysVal: 0.367 ± 0.028
0.103CysTrp: 0.103 ± 0.016
0.242CysTyr: 0.242 ± 0.02
0.0CysXaa: 0.0 ± 0.0
Asp
4.069AspAla: 4.069 ± 0.092
0.329AspCys: 0.329 ± 0.024
4.212AspAsp: 4.212 ± 0.118
3.845AspGlu: 3.845 ± 0.113
2.552AspPhe: 2.552 ± 0.079
4.026AspGly: 4.026 ± 0.109
2.229AspHis: 2.229 ± 0.071
3.477AspIle: 3.477 ± 0.089
3.477AspLys: 3.477 ± 0.093
5.668AspLeu: 5.668 ± 0.12
1.47AspMet: 1.47 ± 0.059
2.992AspAsn: 2.992 ± 0.109
2.665AspPro: 2.665 ± 0.131
3.802AspGln: 3.802 ± 0.102
2.726AspArg: 2.726 ± 0.081
2.966AspSer: 2.966 ± 0.092
2.633AspThr: 2.633 ± 0.076
3.895AspVal: 3.895 ± 0.097
0.773AspTrp: 0.773 ± 0.045
2.478AspTyr: 2.478 ± 0.076
0.0AspXaa: 0.0 ± 0.0
Glu
3.681GluAla: 3.681 ± 0.094
0.232GluCys: 0.232 ± 0.023
2.782GluAsp: 2.782 ± 0.077
2.954GluGlu: 2.954 ± 0.094
1.843GluPhe: 1.843 ± 0.06
2.498GluGly: 2.498 ± 0.07
1.565GluHis: 1.565 ± 0.062
3.346GluIle: 3.346 ± 0.074
3.311GluLys: 3.311 ± 0.1
5.171GluLeu: 5.171 ± 0.126
1.718GluMet: 1.718 ± 0.065
2.429GluAsn: 2.429 ± 0.076
1.615GluPro: 1.615 ± 0.066
2.97GluGln: 2.97 ± 0.08
2.587GluArg: 2.587 ± 0.083
2.292GluSer: 2.292 ± 0.071
2.482GluThr: 2.482 ± 0.078
3.402GluVal: 3.402 ± 0.093
0.521GluTrp: 0.521 ± 0.034
1.759GluTyr: 1.759 ± 0.062
0.0GluXaa: 0.0 ± 0.0
Phe
3.362PheAla: 3.362 ± 0.099
0.315PheCys: 0.315 ± 0.023
2.99PheAsp: 2.99 ± 0.085
1.738PheGlu: 1.738 ± 0.064
1.803PhePhe: 1.803 ± 0.076
3.122PheGly: 3.122 ± 0.088
1.052PheHis: 1.052 ± 0.046
2.891PheIle: 2.891 ± 0.105
2.488PheLys: 2.488 ± 0.076
3.433PheLeu: 3.433 ± 0.104
1.104PheMet: 1.104 ± 0.05
2.201PheAsn: 2.201 ± 0.064
1.472PhePro: 1.472 ± 0.051
1.426PheGln: 1.426 ± 0.055
1.492PheArg: 1.492 ± 0.052
2.609PheSer: 2.609 ± 0.078
2.439PheThr: 2.439 ± 0.068
2.879PheVal: 2.879 ± 0.096
0.515PheTrp: 0.515 ± 0.035
1.514PheTyr: 1.514 ± 0.058
0.0PheXaa: 0.0 ± 0.0
Gly
4.846GlyAla: 4.846 ± 0.125
0.472GlyCys: 0.472 ± 0.034
3.689GlyAsp: 3.689 ± 0.104
3.112GlyGlu: 3.112 ± 0.083
2.928GlyPhe: 2.928 ± 0.078
4.285GlyGly: 4.285 ± 0.104
2.1GlyHis: 2.1 ± 0.072
5.318GlyIle: 5.318 ± 0.109
4.608GlyLys: 4.608 ± 0.108
6.421GlyLeu: 6.421 ± 0.137
2.163GlyMet: 2.163 ± 0.075
2.976GlyAsn: 2.976 ± 0.126
1.71GlyPro: 1.71 ± 0.062
3.394GlyGln: 3.394 ± 0.111
2.974GlyArg: 2.974 ± 0.093
3.774GlySer: 3.774 ± 0.091
4.139GlyThr: 4.139 ± 0.118
4.586GlyVal: 4.586 ± 0.122
1.018GlyTrp: 1.018 ± 0.09
2.74GlyTyr: 2.74 ± 0.099
0.0GlyXaa: 0.0 ± 0.0
His
1.686HisAla: 1.686 ± 0.057
0.174HisCys: 0.174 ± 0.019
1.749HisAsp: 1.749 ± 0.062
1.413HisGlu: 1.413 ± 0.06
1.478HisPhe: 1.478 ± 0.057
1.995HisGly: 1.995 ± 0.071
1.335HisHis: 1.335 ± 0.052
1.526HisIle: 1.526 ± 0.066
1.211HisLys: 1.211 ± 0.052
2.722HisLeu: 2.722 ± 0.085
0.606HisMet: 0.606 ± 0.033
1.343HisAsn: 1.343 ± 0.051
1.484HisPro: 1.484 ± 0.062
1.884HisGln: 1.884 ± 0.063
1.464HisArg: 1.464 ± 0.052
1.518HisSer: 1.518 ± 0.06
1.205HisThr: 1.205 ± 0.045
1.823HisVal: 1.823 ± 0.066
0.416HisTrp: 0.416 ± 0.037
1.367HisTyr: 1.367 ± 0.057
0.0HisXaa: 0.0 ± 0.0
Ile
5.981IleAla: 5.981 ± 0.128
0.561IleCys: 0.561 ± 0.031
4.428IleAsp: 4.428 ± 0.097
3.192IleGlu: 3.192 ± 0.091
2.734IlePhe: 2.734 ± 0.109
4.822IleGly: 4.822 ± 0.134
1.69IleHis: 1.69 ± 0.063
5.117IleIle: 5.117 ± 0.127
4.202IleLys: 4.202 ± 0.098
5.785IleLeu: 5.785 ± 0.161
1.795IleMet: 1.795 ± 0.062
3.976IleAsn: 3.976 ± 0.096
2.694IlePro: 2.694 ± 0.077
2.556IleGln: 2.556 ± 0.074
2.603IleArg: 2.603 ± 0.067
4.232IleSer: 4.232 ± 0.101
4.164IleThr: 4.164 ± 0.083
5.147IleVal: 5.147 ± 0.109
0.66IleTrp: 0.66 ± 0.042
2.308IleTyr: 2.308 ± 0.071
0.0IleXaa: 0.0 ± 0.0
Lys
4.404LysAla: 4.404 ± 0.103
0.22LysCys: 0.22 ± 0.022
3.455LysAsp: 3.455 ± 0.09
3.554LysGlu: 3.554 ± 0.089
1.989LysPhe: 1.989 ± 0.068
3.249LysGly: 3.249 ± 0.094
1.531LysHis: 1.531 ± 0.051
3.845LysIle: 3.845 ± 0.095
4.658LysLys: 4.658 ± 0.13
5.575LysLeu: 5.575 ± 0.111
2.346LysMet: 2.346 ± 0.067
3.055LysAsn: 3.055 ± 0.082
2.362LysPro: 2.362 ± 0.069
3.826LysGln: 3.826 ± 0.099
3.114LysArg: 3.114 ± 0.093
3.037LysSer: 3.037 ± 0.089
3.384LysThr: 3.384 ± 0.09
4.18LysVal: 4.18 ± 0.103
0.723LysTrp: 0.723 ± 0.041
2.502LysTyr: 2.502 ± 0.068
0.0LysXaa: 0.0 ± 0.0
Leu
8.402LeuAla: 8.402 ± 0.143
0.549LeuCys: 0.549 ± 0.034
5.323LeuAsp: 5.323 ± 0.122
3.802LeuGlu: 3.802 ± 0.088
3.772LeuPhe: 3.772 ± 0.111
6.36LeuGly: 6.36 ± 0.12
2.346LeuHis: 2.346 ± 0.07
6.744LeuIle: 6.744 ± 0.166
5.841LeuLys: 5.841 ± 0.126
8.896LeuLeu: 8.896 ± 0.209
2.696LeuMet: 2.696 ± 0.076
4.8LeuAsn: 4.8 ± 0.098
4.186LeuPro: 4.186 ± 0.094
4.091LeuGln: 4.091 ± 0.097
3.956LeuArg: 3.956 ± 0.104
6.062LeuSer: 6.062 ± 0.116
6.413LeuThr: 6.413 ± 0.12
6.716LeuVal: 6.716 ± 0.153
0.874LeuTrp: 0.874 ± 0.048
2.69LeuTyr: 2.69 ± 0.077
0.0LeuXaa: 0.0 ± 0.0
Met
2.623MetAla: 2.623 ± 0.083
0.157MetCys: 0.157 ± 0.017
1.551MetAsp: 1.551 ± 0.061
1.272MetGlu: 1.272 ± 0.053
1.024MetPhe: 1.024 ± 0.049
1.85MetGly: 1.85 ± 0.072
0.575MetHis: 0.575 ± 0.033
2.106MetIle: 2.106 ± 0.071
2.165MetLys: 2.165 ± 0.071
2.399MetLeu: 2.399 ± 0.071
1.016MetMet: 1.016 ± 0.052
1.522MetAsn: 1.522 ± 0.047
1.006MetPro: 1.006 ± 0.042
1.082MetGln: 1.082 ± 0.05
1.113MetArg: 1.113 ± 0.045
1.724MetSer: 1.724 ± 0.057
1.959MetThr: 1.959 ± 0.061
2.005MetVal: 2.005 ± 0.071
0.236MetTrp: 0.236 ± 0.019
0.769MetTyr: 0.769 ± 0.045
0.0MetXaa: 0.0 ± 0.0
Asn
3.721AsnAla: 3.721 ± 0.096
0.267AsnCys: 0.267 ± 0.025
3.334AsnAsp: 3.334 ± 0.096
2.544AsnGlu: 2.544 ± 0.075
1.852AsnPhe: 1.852 ± 0.061
4.014AsnGly: 4.014 ± 0.142
1.654AsnHis: 1.654 ± 0.056
2.908AsnIle: 2.908 ± 0.083
2.873AsnLys: 2.873 ± 0.084
3.931AsnLeu: 3.931 ± 0.089
1.383AsnMet: 1.383 ± 0.045
3.043AsnAsn: 3.043 ± 0.14
2.268AsnPro: 2.268 ± 0.104
2.712AsnGln: 2.712 ± 0.091
2.165AsnArg: 2.165 ± 0.065
2.764AsnSer: 2.764 ± 0.1
2.371AsnThr: 2.371 ± 0.078
3.354AsnVal: 3.354 ± 0.107
0.729AsnTrp: 0.729 ± 0.041
1.981AsnTyr: 1.981 ± 0.074
0.0AsnXaa: 0.0 ± 0.0
Pro
3.206ProAla: 3.206 ± 0.075
0.145ProCys: 0.145 ± 0.018
2.686ProAsp: 2.686 ± 0.126
2.572ProGlu: 2.572 ± 0.08
1.67ProPhe: 1.67 ± 0.063
2.421ProGly: 2.421 ± 0.105
1.016ProHis: 1.016 ± 0.046
2.373ProIle: 2.373 ± 0.072
1.987ProLys: 1.987 ± 0.067
3.639ProLeu: 3.639 ± 0.105
0.874ProMet: 0.874 ± 0.036
1.825ProAsn: 1.825 ± 0.089
0.63ProPro: 0.63 ± 0.036
1.93ProGln: 1.93 ± 0.073
1.341ProArg: 1.341 ± 0.055
2.116ProSer: 2.116 ± 0.064
2.734ProThr: 2.734 ± 0.186
3.277ProVal: 3.277 ± 0.103
0.37ProTrp: 0.37 ± 0.025
1.337ProTyr: 1.337 ± 0.055
0.0ProXaa: 0.0 ± 0.0
Gln
4.071GlnAla: 4.071 ± 0.103
0.172GlnCys: 0.172 ± 0.019
2.259GlnAsp: 2.259 ± 0.071
2.294GlnGlu: 2.294 ± 0.073
1.9GlnPhe: 1.9 ± 0.065
2.542GlnGly: 2.542 ± 0.081
1.484GlnHis: 1.484 ± 0.058
3.574GlnIle: 3.574 ± 0.089
2.879GlnLys: 2.879 ± 0.09
6.021GlnLeu: 6.021 ± 0.16
1.516GlnMet: 1.516 ± 0.058
2.064GlnAsn: 2.064 ± 0.076
2.183GlnPro: 2.183 ± 0.101
3.465GlnGln: 3.465 ± 0.119
2.774GlnArg: 2.774 ± 0.079
2.696GlnSer: 2.696 ± 0.095
2.914GlnThr: 2.914 ± 0.102
3.737GlnVal: 3.737 ± 0.083
0.634GlnTrp: 0.634 ± 0.037
1.88GlnTyr: 1.88 ± 0.062
0.0GlnXaa: 0.0 ± 0.0
Arg
2.954ArgAla: 2.954 ± 0.083
0.202ArgCys: 0.202 ± 0.023
2.356ArgAsp: 2.356 ± 0.076
2.263ArgGlu: 2.263 ± 0.081
1.955ArgPhe: 1.955 ± 0.066
2.572ArgGly: 2.572 ± 0.074
1.545ArgHis: 1.545 ± 0.058
2.835ArgIle: 2.835 ± 0.075
2.506ArgLys: 2.506 ± 0.082
4.422ArgLeu: 4.422 ± 0.111
1.246ArgMet: 1.246 ± 0.058
1.938ArgAsn: 1.938 ± 0.057
1.728ArgPro: 1.728 ± 0.063
3.081ArgGln: 3.081 ± 0.094
2.536ArgArg: 2.536 ± 0.08
2.154ArgSer: 2.154 ± 0.069
2.161ArgThr: 2.161 ± 0.067
2.974ArgVal: 2.974 ± 0.086
0.493ArgTrp: 0.493 ± 0.03
1.747ArgTyr: 1.747 ± 0.057
0.0ArgXaa: 0.0 ± 0.0
Ser
4.64SerAla: 4.64 ± 0.131
0.246SerCys: 0.246 ± 0.023
3.112SerAsp: 3.112 ± 0.091
2.587SerGlu: 2.587 ± 0.078
2.675SerPhe: 2.675 ± 0.078
4.311SerGly: 4.311 ± 0.11
1.508SerHis: 1.508 ± 0.06
3.645SerIle: 3.645 ± 0.088
3.134SerLys: 3.134 ± 0.09
5.64SerLeu: 5.64 ± 0.129
1.454SerMet: 1.454 ± 0.063
2.637SerAsn: 2.637 ± 0.103
2.076SerPro: 2.076 ± 0.078
2.902SerGln: 2.902 ± 0.092
2.427SerArg: 2.427 ± 0.072
3.947SerSer: 3.947 ± 0.159
3.32SerThr: 3.32 ± 0.086
3.643SerVal: 3.643 ± 0.078
0.711SerTrp: 0.711 ± 0.042
2.019SerTyr: 2.019 ± 0.063
0.0SerXaa: 0.0 ± 0.0
Thr
4.844ThrAla: 4.844 ± 0.112
0.315ThrCys: 0.315 ± 0.028
3.481ThrAsp: 3.481 ± 0.113
2.478ThrGlu: 2.478 ± 0.083
2.356ThrPhe: 2.356 ± 0.074
4.636ThrGly: 4.636 ± 0.133
1.207ThrHis: 1.207 ± 0.048
4.632ThrIle: 4.632 ± 0.097
3.622ThrLys: 3.622 ± 0.095
4.977ThrLeu: 4.977 ± 0.109
1.599ThrMet: 1.599 ± 0.058
3.059ThrAsn: 3.059 ± 0.091
2.74ThrPro: 2.74 ± 0.151
1.955ThrGln: 1.955 ± 0.095
1.969ThrArg: 1.969 ± 0.063
3.398ThrSer: 3.398 ± 0.092
3.865ThrThr: 3.865 ± 0.128
4.67ThrVal: 4.67 ± 0.14
0.628ThrTrp: 0.628 ± 0.037
1.963ThrTyr: 1.963 ± 0.075
0.0ThrXaa: 0.0 ± 0.0
Val
6.203ValAla: 6.203 ± 0.132
0.491ValCys: 0.491 ± 0.035
4.584ValAsp: 4.584 ± 0.134
3.34ValGlu: 3.34 ± 0.087
2.425ValPhe: 2.425 ± 0.072
4.884ValGly: 4.884 ± 0.111
1.71ValHis: 1.71 ± 0.062
5.482ValIle: 5.482 ± 0.121
4.448ValLys: 4.448 ± 0.083
6.35ValLeu: 6.35 ± 0.156
1.819ValMet: 1.819 ± 0.063
3.713ValAsn: 3.713 ± 0.094
2.916ValPro: 2.916 ± 0.093
2.801ValGln: 2.801 ± 0.077
2.617ValArg: 2.617 ± 0.079
4.054ValSer: 4.054 ± 0.097
4.796ValThr: 4.796 ± 0.168
5.466ValVal: 5.466 ± 0.134
0.687ValTrp: 0.687 ± 0.04
2.15ValTyr: 2.15 ± 0.065
0.0ValXaa: 0.0 ± 0.0
Trp
0.8TrpAla: 0.8 ± 0.052
0.109TrpCys: 0.109 ± 0.013
0.561TrpAsp: 0.561 ± 0.039
0.39TrpGlu: 0.39 ± 0.029
0.614TrpPhe: 0.614 ± 0.04
0.672TrpGly: 0.672 ± 0.035
0.333TrpHis: 0.333 ± 0.028
0.747TrpIle: 0.747 ± 0.042
0.489TrpLys: 0.489 ± 0.032
1.476TrpLeu: 1.476 ± 0.07
0.321TrpMet: 0.321 ± 0.025
0.479TrpAsn: 0.479 ± 0.037
0.325TrpPro: 0.325 ± 0.029
0.886TrpGln: 0.886 ± 0.058
0.575TrpArg: 0.575 ± 0.034
0.678TrpSer: 0.678 ± 0.036
0.571TrpThr: 0.571 ± 0.037
0.664TrpVal: 0.664 ± 0.038
0.25TrpTrp: 0.25 ± 0.021
0.66TrpTyr: 0.66 ± 0.088
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.476TyrAla: 2.476 ± 0.073
0.309TyrCys: 0.309 ± 0.028
2.221TyrAsp: 2.221 ± 0.066
1.617TyrGlu: 1.617 ± 0.055
1.856TyrPhe: 1.856 ± 0.087
2.467TyrGly: 2.467 ± 0.072
1.29TyrHis: 1.29 ± 0.052
2.019TyrIle: 2.019 ± 0.072
1.569TyrLys: 1.569 ± 0.075
3.984TyrLeu: 3.984 ± 0.089
0.767TyrMet: 0.767 ± 0.041
1.611TyrAsn: 1.611 ± 0.065
1.424TyrPro: 1.424 ± 0.057
2.332TyrGln: 2.332 ± 0.087
1.872TyrArg: 1.872 ± 0.077
2.058TyrSer: 2.058 ± 0.064
1.85TyrThr: 1.85 ± 0.077
2.35TyrVal: 2.35 ± 0.085
0.505TyrTrp: 0.505 ± 0.035
1.64TyrTyr: 1.64 ± 0.101
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1641 proteins (504748 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski