Gyrovirus Tu789
Average proteome isoelectric point is 7.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U5UAJ3|U5UAJ3_9VIRU Apoptin OS=Gyrovirus Tu789 OX=1415628 PE=3 SV=1
MM1 pKa = 7.57 SSGGVEE7 pKa = 3.92 STPPGEE13 pKa = 4.05 TRR15 pKa = 11.84 PAGGSEE21 pKa = 4.1 SPFRR25 pKa = 11.84 RR26 pKa = 11.84 EE27 pKa = 3.91 GQRR30 pKa = 11.84 GPSGAGYY37 pKa = 9.29 PGEE40 pKa = 4.26 TLTPSPRR47 pKa = 11.84 GHH49 pKa = 5.22 VRR51 pKa = 11.84 KK52 pKa = 9.96 HH53 pKa = 5.59 IDD55 pKa = 4.23 GIVASNKK62 pKa = 9.1 FVSVGWDD69 pKa = 3.08 SLQRR73 pKa = 11.84 DD74 pKa = 4.39 PNWTRR79 pKa = 11.84 QCYY82 pKa = 9.25 NRR84 pKa = 11.84 AIASWLRR91 pKa = 11.84 NCARR95 pKa = 11.84 THH97 pKa = 7.67 DD98 pKa = 4.69 EE99 pKa = 4.4 ICNCGQWRR107 pKa = 11.84 RR108 pKa = 11.84 HH109 pKa = 4.91 WFQEE113 pKa = 4.31 CAGLEE118 pKa = 4.22 DD119 pKa = 5.15 ASSQTEE125 pKa = 4.03 DD126 pKa = 3.33 PRR128 pKa = 11.84 AQEE131 pKa = 3.71 DD132 pKa = 3.66 LRR134 pKa = 11.84 RR135 pKa = 11.84 LRR137 pKa = 11.84 EE138 pKa = 3.79 SGAAAKK144 pKa = 10.27 RR145 pKa = 11.84 KK146 pKa = 9.47 LDD148 pKa = 4.0 YY149 pKa = 10.43 IDD151 pKa = 3.87 RR152 pKa = 11.84 CKK154 pKa = 10.58 KK155 pKa = 10.06 RR156 pKa = 11.84 PKK158 pKa = 9.0 TVTWFDD164 pKa = 3.24 TGEE167 pKa = 4.11 EE168 pKa = 4.16 PDD170 pKa = 3.62 VPEE173 pKa = 4.95 GFFTPSEE180 pKa = 4.47 DD181 pKa = 3.43 EE182 pKa = 4.79 DD183 pKa = 6.34 GYY185 pKa = 10.88 DD186 pKa = 3.43 TDD188 pKa = 3.51 VDD190 pKa = 3.64 EE191 pKa = 5.35 DD192 pKa = 4.07 AVPGGVNFDD201 pKa = 3.95 MRR203 pKa = 11.84 VEE205 pKa = 4.26 DD206 pKa = 4.09 PFLNALRR213 pKa = 11.84 GGSTTRR219 pKa = 11.84 IQGPTWW225 pKa = 3.22
Molecular weight: 25.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.151
IPC2_protein 5.194
IPC_protein 5.118
Toseland 5.054
ProMoST 5.27
Dawson 5.118
Bjellqvist 5.245
Wikipedia 5.003
Rodwell 5.029
Grimsley 4.978
Solomon 5.118
Lehninger 5.08
Nozaki 5.245
DTASelect 5.41
Thurlkill 5.067
EMBOSS 5.041
Sillero 5.308
Patrickios 4.291
IPC_peptide 5.13
IPC2_peptide 5.308
IPC2.peptide.svr19 5.313
Protein with the highest isoelectric point:
>tr|U5UA55|U5UA55_9VIRU Dual specificity protein phosphatase VP2 OS=Gyrovirus Tu789 OX=1415628 PE=3 SV=1
MM1 pKa = 6.6 VRR3 pKa = 11.84 YY4 pKa = 9.35 RR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 ARR9 pKa = 11.84 RR10 pKa = 11.84 PRR12 pKa = 11.84 GVFYY16 pKa = 11.07 SLGRR20 pKa = 11.84 RR21 pKa = 11.84 GWIRR25 pKa = 11.84 HH26 pKa = 3.82 RR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 TWWGKK38 pKa = 8.44 FRR40 pKa = 11.84 HH41 pKa = 5.05 ARR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 SFSQRR50 pKa = 11.84 VKK52 pKa = 10.66 RR53 pKa = 11.84 RR54 pKa = 11.84 FHH56 pKa = 6.18 NPHH59 pKa = 6.89 PGTYY63 pKa = 9.85 LVRR66 pKa = 11.84 LPRR69 pKa = 11.84 PQTTLTIFFQGIVYY83 pKa = 9.57 FPTAKK88 pKa = 9.68 MYY90 pKa = 10.85 ASRR93 pKa = 11.84 NQLADD98 pKa = 3.63 LNKK101 pKa = 9.23 ITTCRR106 pKa = 11.84 VGLMNINFRR115 pKa = 11.84 EE116 pKa = 4.14 FLLAALPLDD125 pKa = 4.57 AYY127 pKa = 10.77 SKK129 pKa = 10.87 LGGPIVGPQYY139 pKa = 11.15 VSGGGSSAGTHH150 pKa = 7.04 DD151 pKa = 3.34 EE152 pKa = 4.4 TMWPFTARR160 pKa = 11.84 PVTNKK165 pKa = 9.83 QPGASPAEE173 pKa = 3.89 WWRR176 pKa = 11.84 WALLLMFPRR185 pKa = 11.84 EE186 pKa = 3.78 PNMFFRR192 pKa = 11.84 QPQQPTLEE200 pKa = 4.06 QMGEE204 pKa = 4.01 IFGGWQLYY212 pKa = 8.37 RR213 pKa = 11.84 HH214 pKa = 6.77 IKK216 pKa = 8.46 TKK218 pKa = 9.5 VAIMAAQDD226 pKa = 3.32 GGAFSPVASLVSQNNYY242 pKa = 6.08 FWRR245 pKa = 11.84 RR246 pKa = 11.84 QEE248 pKa = 3.83 RR249 pKa = 11.84 GTPKK253 pKa = 10.0 TGEE256 pKa = 3.9 PPMTGMIRR264 pKa = 11.84 RR265 pKa = 11.84 GTGSLEE271 pKa = 3.89 SSKK274 pKa = 10.8 PADD277 pKa = 3.74 EE278 pKa = 4.79 YY279 pKa = 11.38 YY280 pKa = 10.3 IPANPPNPPDD290 pKa = 3.38 WPGEE294 pKa = 4.03 SSAVIDD300 pKa = 3.91 TPYY303 pKa = 10.56 KK304 pKa = 9.35 IAAVRR309 pKa = 11.84 SKK311 pKa = 11.03 SYY313 pKa = 10.74 VYY315 pKa = 11.39 SNMLFPSFSALSALGAAWAFPPGRR339 pKa = 11.84 RR340 pKa = 11.84 SVGRR344 pKa = 11.84 GSFNRR349 pKa = 11.84 HH350 pKa = 5.65 TITGAGDD357 pKa = 3.53 PQGKK361 pKa = 9.08 KK362 pKa = 8.82 WLTLVPKK369 pKa = 9.85 KK370 pKa = 10.95 VEE372 pKa = 4.33 WITDD376 pKa = 3.41 DD377 pKa = 3.76 TMTQSEE383 pKa = 4.91 IDD385 pKa = 3.45 TTIATIYY392 pKa = 8.98 VAQGAPKK399 pKa = 9.73 ASGYY403 pKa = 10.5 KK404 pKa = 9.78 FQTFQGPLVDD414 pKa = 5.69 DD415 pKa = 4.75 PLSVQPWAVMRR426 pKa = 11.84 VKK428 pKa = 10.67 SVWQLGNLRR437 pKa = 11.84 RR438 pKa = 11.84 PYY440 pKa = 9.58 PWDD443 pKa = 3.54 VNWYY447 pKa = 9.18 NQVADD452 pKa = 3.51 WW453 pKa = 4.03
Molecular weight: 51.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.31
IPC2_protein 10.058
IPC_protein 11.038
Toseland 10.979
ProMoST 10.979
Dawson 11.067
Bjellqvist 10.877
Wikipedia 11.374
Rodwell 11.125
Grimsley 11.125
Solomon 11.286
Lehninger 11.228
Nozaki 10.965
DTASelect 10.877
Thurlkill 10.979
EMBOSS 11.418
Sillero 11.008
Patrickios 10.804
IPC_peptide 11.301
IPC2_peptide 10.028
IPC2.peptide.svr19 8.334
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
789
111
453
263.0
29.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.478 ± 0.476
1.394 ± 0.944
4.816 ± 1.359
4.563 ± 1.397
3.802 ± 1.045
8.745 ± 0.689
1.521 ± 0.145
3.676 ± 0.362
3.676 ± 0.417
6.337 ± 1.264
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.028 ± 0.659
3.295 ± 0.202
7.858 ± 0.942
4.056 ± 0.71
10.266 ± 0.103
7.605 ± 1.458
7.351 ± 1.265
5.577 ± 0.253
3.169 ± 0.839
2.788 ± 0.995
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here