Pseudomonas sp. M30-35
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4347 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y0KRY8|A0A1Y0KRY8_9PSED GpE family phage tail protein OS=Pseudomonas sp. M30-35 OX=1981174 GN=B9K09_10030 PE=4 SV=1
MM1 pKa = 6.85 KK2 pKa = 10.14 TSIEE6 pKa = 4.29 VIDD9 pKa = 4.1 RR10 pKa = 11.84 ATSNNINMIADD21 pKa = 3.77 GNLAISLNTPSVVLVHH37 pKa = 6.63 GSVADD42 pKa = 3.3 VDD44 pKa = 3.94 RR45 pKa = 11.84 YY46 pKa = 10.78 EE47 pKa = 4.07 RR48 pKa = 11.84 TGNDD52 pKa = 3.34 LLIYY56 pKa = 9.41 MKK58 pKa = 10.31 DD59 pKa = 3.43 GSVIRR64 pKa = 11.84 CDD66 pKa = 4.14 GYY68 pKa = 10.46 FQVDD72 pKa = 3.7 EE73 pKa = 4.68 TEE75 pKa = 4.08 HH76 pKa = 7.51 HH77 pKa = 6.59 SEE79 pKa = 5.77 LVFQDD84 pKa = 3.78 DD85 pKa = 3.88 NSALTHH91 pKa = 6.46 ISFADD96 pKa = 3.8 MPEE99 pKa = 3.96 VAPDD103 pKa = 3.58 QIVPLQAQIEE113 pKa = 4.39 MLDD116 pKa = 3.45 KK117 pKa = 10.97 VDD119 pKa = 3.85 PLLYY123 pKa = 10.79 GSDD126 pKa = 3.67 DD127 pKa = 3.57 SSGYY131 pKa = 10.84 GLLGLMGAGLGGALVGAVLNNDD153 pKa = 3.34 EE154 pKa = 5.49 DD155 pKa = 4.08 GGSNKK160 pKa = 9.34 VVTIDD165 pKa = 3.35 NTLPPEE171 pKa = 4.25 TAPPTFIATDD181 pKa = 3.55 NQGQLQTLLVNGSKK195 pKa = 9.47 TDD197 pKa = 3.62 DD198 pKa = 3.81 LSPVFSGSGQAGANIQIVNVEE219 pKa = 4.18 GVVIASTQVGAGGTWTVTAPDD240 pKa = 3.45 QALGQHH246 pKa = 5.83 TYY248 pKa = 10.78 EE249 pKa = 4.49 VVQQSGGNSLSAGTVSFDD267 pKa = 3.06 IVGNTVEE274 pKa = 4.26 LTIDD278 pKa = 3.51 PVGGDD283 pKa = 3.5 NQIDD287 pKa = 3.92 EE288 pKa = 5.09 SEE290 pKa = 4.32 VSAALIVSGSATGLEE305 pKa = 4.37 TGTEE309 pKa = 4.46 VIVSIADD316 pKa = 3.32 KK317 pKa = 10.94 SYY319 pKa = 7.57 TTQIIDD325 pKa = 3.4 GGLWSLEE332 pKa = 4.04 LPSEE336 pKa = 4.67 VVAGLHH342 pKa = 6.57 DD343 pKa = 4.47 GFYY346 pKa = 9.35 TAQVSATDD354 pKa = 3.77 LAGNGHH360 pKa = 5.68 TASKK364 pKa = 10.27 GFSVDD369 pKa = 2.57 TSAPVVSINAVALDD383 pKa = 3.79 DD384 pKa = 3.94 VLNASEE390 pKa = 4.5 VSTTQTISGSVMGAAAGEE408 pKa = 4.42 TVTVNVGGTQYY419 pKa = 10.92 QGLLGIDD426 pKa = 3.74 NTWSVDD432 pKa = 3.13 IPSSMWTTIGDD443 pKa = 3.83 GALTISASTVDD454 pKa = 3.27 AAGNTGEE461 pKa = 4.13 ASRR464 pKa = 11.84 DD465 pKa = 3.11 ISIAAGLPGLRR476 pKa = 11.84 VATVSGDD483 pKa = 3.92 DD484 pKa = 3.61 IINAIEE490 pKa = 4.05 HH491 pKa = 6.16 AQDD494 pKa = 3.26 LTIFGTSTGLVAGQTVTATIDD515 pKa = 3.34 GKK517 pKa = 11.43 SYY519 pKa = 9.49 LTAINADD526 pKa = 3.73 GSWQVGLQASDD537 pKa = 3.12 VSKK540 pKa = 11.18 YY541 pKa = 7.43 MQGLLVIHH549 pKa = 6.46 VSSADD554 pKa = 3.29 ASGNPVAIEE563 pKa = 3.85 HH564 pKa = 6.29 QIMVDD569 pKa = 3.6 LADD572 pKa = 3.56 TAVSINAVALDD583 pKa = 3.98 NIINSVEE590 pKa = 4.02 QASDD594 pKa = 3.72 LLLSGATANVEE605 pKa = 3.87 AGQVVVINFAGHH617 pKa = 5.81 QYY619 pKa = 10.66 LATVAGDD626 pKa = 3.92 GGWSVVLPATDD637 pKa = 4.62 LGGLTDD643 pKa = 4.54 GYY645 pKa = 11.24 RR646 pKa = 11.84 NLEE649 pKa = 4.13 VQVTNSTGSVAHH661 pKa = 6.46 SEE663 pKa = 4.05 QLVQVDD669 pKa = 4.02 VTSPILVIGPAVSSGGLDD687 pKa = 3.44 SAHH690 pKa = 7.68 DD691 pKa = 4.14 DD692 pKa = 3.83 LLNALEE698 pKa = 4.31 VNQDD702 pKa = 3.88 LLVSGVTSAQVDD714 pKa = 3.57 QALTVSLNGQSYY726 pKa = 6.56 QTVVGGGGVWSVTIPSTNFSSAPDD750 pKa = 3.58 GALLITATTTDD761 pKa = 3.21 KK762 pKa = 11.38 AGNSVLQTRR771 pKa = 11.84 EE772 pKa = 3.98 LTVDD776 pKa = 3.52 TTAPVITINTVAGDD790 pKa = 3.71 NVIDD794 pKa = 3.99 GTEE797 pKa = 3.84 QKK799 pKa = 10.6 AGQLISGTADD809 pKa = 3.3 TEE811 pKa = 4.21 PGQVVEE817 pKa = 4.34 VTFNGRR823 pKa = 11.84 TYY825 pKa = 10.91 QSVVADD831 pKa = 4.38 GGAWSVDD838 pKa = 2.44 VDD840 pKa = 4.15 ARR842 pKa = 11.84 DD843 pKa = 4.33 FLDD846 pKa = 4.53 TPDD849 pKa = 3.62 GDD851 pKa = 4.27 YY852 pKa = 10.99 DD853 pKa = 3.48 ITASVVDD860 pKa = 3.72 KK861 pKa = 11.04 AGNAGATNGSVYY873 pKa = 10.53 LQADD877 pKa = 4.02 APTVTINSFTADD889 pKa = 4.35 DD890 pKa = 3.97 ILSKK894 pKa = 10.94 LEE896 pKa = 3.69 QGAPQTLSGTSSVDD910 pKa = 2.68 AGRR913 pKa = 11.84 VVTVTLNGKK922 pKa = 9.78 DD923 pKa = 3.36 YY924 pKa = 10.61 SAPVQGDD931 pKa = 3.73 HH932 pKa = 6.26 SWSVVLGTADD942 pKa = 3.87 LAALADD948 pKa = 3.76 SGVYY952 pKa = 10.38 SITATATDD960 pKa = 3.63 NVGNTGSSDD969 pKa = 3.07 HH970 pKa = 6.78 SFTVDD975 pKa = 3.31 LSGPLMTLTIDD986 pKa = 4.45 SITVDD991 pKa = 3.89 SGPSSSDD998 pKa = 3.33 FITNDD1003 pKa = 2.54 TSLAVQGTLGAEE1015 pKa = 4.4 LGAGEE1020 pKa = 4.97 LMQISLDD1027 pKa = 4.99 GGATWINLHH1036 pKa = 5.99 VSGTTWTYY1044 pKa = 10.4 VDD1046 pKa = 4.36 SRR1048 pKa = 11.84 TLGDD1052 pKa = 3.44 GTHH1055 pKa = 7.6 DD1056 pKa = 3.56 YY1057 pKa = 10.07 TVRR1060 pKa = 11.84 LIDD1063 pKa = 3.4 SLGNVGATASQLVTIDD1079 pKa = 3.52 TNAPTSTIAITSISDD1094 pKa = 3.41 DD1095 pKa = 3.33 TGSYY1099 pKa = 10.35 VDD1101 pKa = 5.29 YY1102 pKa = 9.65 ITNDD1106 pKa = 2.75 TSLTVNGTLSSPLAVDD1122 pKa = 3.85 EE1123 pKa = 4.59 LAQISLDD1130 pKa = 4.44 NGATWTALSIAGTTWSHH1147 pKa = 6.52 ADD1149 pKa = 3.71 DD1150 pKa = 3.75 RR1151 pKa = 11.84 TLVDD1155 pKa = 4.08 GSYY1158 pKa = 10.36 QYY1160 pKa = 9.55 TVRR1163 pKa = 11.84 VIDD1166 pKa = 3.53 VAGSIGSTYY1175 pKa = 8.16 TQTVIVDD1182 pKa = 3.89 TAAPVAAIHH1191 pKa = 6.77 IDD1193 pKa = 3.94 SITDD1197 pKa = 3.44 DD1198 pKa = 3.94 TGVSATDD1205 pKa = 4.39 YY1206 pKa = 9.43 ITSDD1210 pKa = 2.84 NTLVVNGTLSAALNTDD1226 pKa = 4.39 EE1227 pKa = 4.93 IAQISLDD1234 pKa = 3.74 GGAVWTALVTSGLAWNYY1251 pKa = 9.46 TDD1253 pKa = 5.81 GRR1255 pKa = 11.84 TLSDD1259 pKa = 3.52 GVHH1262 pKa = 6.46 TYY1264 pKa = 7.34 QVRR1267 pKa = 11.84 VVDD1270 pKa = 4.13 LAGNSGPVEE1279 pKa = 4.09 QQAVTVDD1286 pKa = 3.72 RR1287 pKa = 11.84 VAPTTSISIDD1297 pKa = 3.9 SISDD1301 pKa = 3.45 DD1302 pKa = 3.7 TGLSLTDD1309 pKa = 4.57 FITRR1313 pKa = 11.84 DD1314 pKa = 3.27 TSLTLSGTLGKK1325 pKa = 10.39 ALEE1328 pKa = 4.36 SDD1330 pKa = 3.78 EE1331 pKa = 4.97 LVQISIDD1338 pKa = 4.89 AGTTWITLTDD1348 pKa = 3.44 VTGTQWGYY1356 pKa = 9.95 IDD1358 pKa = 5.46 SRR1360 pKa = 11.84 VLTDD1364 pKa = 4.0 GNYY1367 pKa = 10.22 DD1368 pKa = 3.43 YY1369 pKa = 11.05 QLRR1372 pKa = 11.84 VIDD1375 pKa = 3.53 QAGNIGQTASQTVVVDD1391 pKa = 4.87 TIAPLEE1397 pKa = 3.93 QATITGYY1404 pKa = 11.16 DD1405 pKa = 3.61 DD1406 pKa = 4.62 DD1407 pKa = 3.86 QGARR1411 pKa = 11.84 QGIQTTHH1418 pKa = 7.02 NITDD1422 pKa = 3.38 DD1423 pKa = 3.85 TAPVLQGTLTAEE1435 pKa = 4.19 LQGGEE1440 pKa = 4.16 VLKK1443 pKa = 10.69 IFQNGIYY1450 pKa = 10.06 LGVATVSGGTWAYY1463 pKa = 10.95 ALTGLEE1469 pKa = 3.97 NANTYY1474 pKa = 10.54 DD1475 pKa = 3.55 YY1476 pKa = 11.41 SAVVTDD1482 pKa = 3.98 AAGNTTTSADD1492 pKa = 3.19 YY1493 pKa = 10.56 RR1494 pKa = 11.84 LNVDD1498 pKa = 3.4 TSVPSSVTFEE1508 pKa = 4.14 SQITSDD1514 pKa = 3.58 TTPVLRR1520 pKa = 11.84 GTILEE1525 pKa = 4.81 DD1526 pKa = 3.98 LSASQILYY1534 pKa = 8.49 ITVNGITYY1542 pKa = 10.38 SSSTSYY1548 pKa = 11.21 GDD1550 pKa = 3.16 GRR1552 pKa = 11.84 VVVDD1556 pKa = 3.36 TANRR1560 pKa = 11.84 TWYY1563 pKa = 9.73 VQIPSSDD1570 pKa = 3.29 NLGIDD1575 pKa = 3.6 TYY1577 pKa = 11.59 DD1578 pKa = 3.65 VIAKK1582 pKa = 8.79 VVNEE1586 pKa = 4.58 DD1587 pKa = 3.79 SNGSLSSEE1595 pKa = 4.42 TFSGAQVVIAEE1606 pKa = 4.39 PAIDD1610 pKa = 3.95 TQWAGDD1616 pKa = 3.75 APGEE1620 pKa = 4.06 RR1621 pKa = 11.84 TNNQEE1626 pKa = 3.8 GLSYY1630 pKa = 10.88 SIGEE1634 pKa = 3.96 NGLWNIVANTAVYY1647 pKa = 10.57 NSSDD1651 pKa = 3.77 LNTYY1655 pKa = 8.48 STDD1658 pKa = 3.31 VLAKK1662 pKa = 10.44 EE1663 pKa = 4.44 SVTGNTVNYY1672 pKa = 9.67 TYY1674 pKa = 11.11 FDD1676 pKa = 4.48 LDD1678 pKa = 3.51 RR1679 pKa = 11.84 DD1680 pKa = 3.34 GDD1682 pKa = 3.63 MDD1684 pKa = 4.03 IFATQTSYY1692 pKa = 10.45 TRR1694 pKa = 11.84 PFQYY1698 pKa = 9.16 WQNDD1702 pKa = 3.23 NGSYY1706 pKa = 8.71 TSKK1709 pKa = 9.55 TIEE1712 pKa = 3.95 YY1713 pKa = 9.13 GALDD1717 pKa = 4.09 TYY1719 pKa = 11.6 YY1720 pKa = 10.54 GGTVAYY1726 pKa = 10.1 DD1727 pKa = 3.41 RR1728 pKa = 11.84 TGNGYY1733 pKa = 10.49 LDD1735 pKa = 4.56 LVLGDD1740 pKa = 4.83 SNLDD1744 pKa = 3.75 SYY1746 pKa = 12.01 SSGWFNNNQDD1756 pKa = 3.35 GTFTQDD1762 pKa = 2.45 TVTRR1766 pKa = 11.84 GLTPSYY1772 pKa = 11.1 SFGASISGVDD1782 pKa = 3.5 INNDD1786 pKa = 2.97 GTVDD1790 pKa = 2.87 ITGQMNGGGGSNGYY1804 pKa = 7.26 TLGVITNNGDD1814 pKa = 2.73 GTMAIGQIVNNVFNDD1829 pKa = 3.36 GGGADD1834 pKa = 3.65 NVGTSMTWADD1844 pKa = 3.76 FNGDD1848 pKa = 3.5 GTLDD1852 pKa = 3.64 LYY1854 pKa = 11.43 LNQHH1858 pKa = 6.17 FAGANKK1864 pKa = 10.09 AAVLINNNGTLDD1876 pKa = 3.56 NFVEE1880 pKa = 4.79 VNAGPDD1886 pKa = 3.35 VRR1888 pKa = 11.84 SRR1890 pKa = 11.84 IALAVDD1896 pKa = 3.34 WDD1898 pKa = 4.35 HH1899 pKa = 7.51 NGTTDD1904 pKa = 2.99 IAKK1907 pKa = 9.58 MGGYY1911 pKa = 10.45 NNGDD1915 pKa = 4.15 TIQMILNNGNGLSWTNANLNYY1936 pKa = 10.21 SYY1938 pKa = 11.32 SSRR1941 pKa = 11.84 LTGAAAMDD1949 pKa = 4.32 YY1950 pKa = 10.47 DD1951 pKa = 3.47 WDD1953 pKa = 4.14 GAVDD1957 pKa = 6.23 LIGFTNNGHH1966 pKa = 5.55 SFVVKK1971 pKa = 9.17 NTNAVAEE1978 pKa = 4.62 GTSMHH1983 pKa = 6.49 LRR1985 pKa = 11.84 IVDD1988 pKa = 3.55 QNGINAFYY1996 pKa = 10.85 GNTVQLFDD2004 pKa = 3.75 SSGALVATQILNAQSGVGVNDD2025 pKa = 3.59 SSALLNFYY2033 pKa = 10.44 GLSASEE2039 pKa = 4.39 TYY2041 pKa = 10.42 SVNLIRR2047 pKa = 11.84 IVDD2050 pKa = 4.13 GVRR2053 pKa = 11.84 SDD2055 pKa = 3.35 VTEE2058 pKa = 4.2 TVNSGWGGLTTGDD2071 pKa = 3.24 ATHH2074 pKa = 6.83 NYY2076 pKa = 8.11 VLSVEE2081 pKa = 4.33 SDD2083 pKa = 2.96 SDD2085 pKa = 3.7 RR2086 pKa = 11.84 NNGVFVGSGYY2096 pKa = 10.79 NDD2098 pKa = 3.43 TFIATAGSDD2107 pKa = 3.58 TYY2109 pKa = 11.45 NGGGGWSYY2117 pKa = 11.43 SSEE2120 pKa = 3.92 HH2121 pKa = 5.69 GTWVAANGGMDD2132 pKa = 3.39 VVDD2135 pKa = 3.84 YY2136 pKa = 11.09 QLATNGVTVDD2146 pKa = 4.2 LSSTATQDD2154 pKa = 2.98 TGFNSAKK2161 pKa = 10.27 LVDD2164 pKa = 3.88 VEE2166 pKa = 4.75 GVLGSSFDD2174 pKa = 5.04 DD2175 pKa = 3.52 IFTGSAGDD2183 pKa = 3.46 NQFQGRR2189 pKa = 11.84 GGNDD2193 pKa = 4.55 LINIANGGHH2202 pKa = 7.34 DD2203 pKa = 3.59 SLIYY2207 pKa = 10.56 NLLSAVDD2214 pKa = 3.66 ATGGNGTDD2222 pKa = 4.08 AIAGFTVGTWEE2233 pKa = 4.22 GTNDD2237 pKa = 3.51 TDD2239 pKa = 5.73 RR2240 pKa = 11.84 IDD2242 pKa = 3.59 INEE2245 pKa = 4.35 LLHH2248 pKa = 5.8 SAGYY2252 pKa = 8.43 TGTGSASYY2260 pKa = 10.91 VDD2262 pKa = 5.02 GVATLDD2268 pKa = 3.48 TSAGNIEE2275 pKa = 5.17 DD2276 pKa = 4.07 YY2277 pKa = 11.35 LSVLQNGANTDD2288 pKa = 3.05 IQFDD2292 pKa = 3.82 HH2293 pKa = 7.59 DD2294 pKa = 4.24 GLGGAFDD2301 pKa = 4.03 PTTIASLSGVQVDD2314 pKa = 5.43 LATLLANHH2322 pKa = 6.16 QLLVAA2327 pKa = 5.06
Molecular weight: 241.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.696
IPC2_protein 3.605
IPC_protein 3.681
Toseland 3.427
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.643
Rodwell 3.49
Grimsley 3.325
Solomon 3.681
Lehninger 3.643
Nozaki 3.783
DTASelect 4.101
Thurlkill 3.49
EMBOSS 3.656
Sillero 3.795
Patrickios 0.858
IPC_peptide 3.668
IPC2_peptide 3.77
IPC2.peptide.svr19 3.715
Protein with the highest isoelectric point:
>tr|A0A1Y0KMC8|A0A1Y0KMC8_9PSED Pyrroline-5-carboxylate reductase OS=Pseudomonas sp. M30-35 OX=1981174 GN=proC PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.56 RR3 pKa = 11.84 TFQPSTIKK11 pKa = 10.52 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.94 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.16 NGRR28 pKa = 11.84 AVLSRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.38 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LAVV44 pKa = 3.42
Molecular weight: 5.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4347
0
4347
1427656
38
4652
328.4
36.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.64 ± 0.041
1.023 ± 0.013
5.371 ± 0.042
5.751 ± 0.039
3.716 ± 0.028
7.719 ± 0.047
2.138 ± 0.018
5.221 ± 0.029
3.75 ± 0.033
11.582 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.419 ± 0.021
3.326 ± 0.028
4.625 ± 0.026
4.679 ± 0.033
5.831 ± 0.033
6.24 ± 0.032
4.951 ± 0.038
7.052 ± 0.032
1.388 ± 0.016
2.58 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here