Pseudomonas sp. M30-35

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; unclassified Pseudomonas

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4347 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1Y0KRY8|A0A1Y0KRY8_9PSED GpE family phage tail protein OS=Pseudomonas sp. M30-35 OX=1981174 GN=B9K09_10030 PE=4 SV=1
MM1 pKa = 6.85KK2 pKa = 10.14TSIEE6 pKa = 4.29VIDD9 pKa = 4.1RR10 pKa = 11.84ATSNNINMIADD21 pKa = 3.77GNLAISLNTPSVVLVHH37 pKa = 6.63GSVADD42 pKa = 3.3VDD44 pKa = 3.94RR45 pKa = 11.84YY46 pKa = 10.78EE47 pKa = 4.07RR48 pKa = 11.84TGNDD52 pKa = 3.34LLIYY56 pKa = 9.41MKK58 pKa = 10.31DD59 pKa = 3.43GSVIRR64 pKa = 11.84CDD66 pKa = 4.14GYY68 pKa = 10.46FQVDD72 pKa = 3.7EE73 pKa = 4.68TEE75 pKa = 4.08HH76 pKa = 7.51HH77 pKa = 6.59SEE79 pKa = 5.77LVFQDD84 pKa = 3.78DD85 pKa = 3.88NSALTHH91 pKa = 6.46ISFADD96 pKa = 3.8MPEE99 pKa = 3.96VAPDD103 pKa = 3.58QIVPLQAQIEE113 pKa = 4.39MLDD116 pKa = 3.45KK117 pKa = 10.97VDD119 pKa = 3.85PLLYY123 pKa = 10.79GSDD126 pKa = 3.67DD127 pKa = 3.57SSGYY131 pKa = 10.84GLLGLMGAGLGGALVGAVLNNDD153 pKa = 3.34EE154 pKa = 5.49DD155 pKa = 4.08GGSNKK160 pKa = 9.34VVTIDD165 pKa = 3.35NTLPPEE171 pKa = 4.25TAPPTFIATDD181 pKa = 3.55NQGQLQTLLVNGSKK195 pKa = 9.47TDD197 pKa = 3.62DD198 pKa = 3.81LSPVFSGSGQAGANIQIVNVEE219 pKa = 4.18GVVIASTQVGAGGTWTVTAPDD240 pKa = 3.45QALGQHH246 pKa = 5.83TYY248 pKa = 10.78EE249 pKa = 4.49VVQQSGGNSLSAGTVSFDD267 pKa = 3.06IVGNTVEE274 pKa = 4.26LTIDD278 pKa = 3.51PVGGDD283 pKa = 3.5NQIDD287 pKa = 3.92EE288 pKa = 5.09SEE290 pKa = 4.32VSAALIVSGSATGLEE305 pKa = 4.37TGTEE309 pKa = 4.46VIVSIADD316 pKa = 3.32KK317 pKa = 10.94SYY319 pKa = 7.57TTQIIDD325 pKa = 3.4GGLWSLEE332 pKa = 4.04LPSEE336 pKa = 4.67VVAGLHH342 pKa = 6.57DD343 pKa = 4.47GFYY346 pKa = 9.35TAQVSATDD354 pKa = 3.77LAGNGHH360 pKa = 5.68TASKK364 pKa = 10.27GFSVDD369 pKa = 2.57TSAPVVSINAVALDD383 pKa = 3.79DD384 pKa = 3.94VLNASEE390 pKa = 4.5VSTTQTISGSVMGAAAGEE408 pKa = 4.42TVTVNVGGTQYY419 pKa = 10.92QGLLGIDD426 pKa = 3.74NTWSVDD432 pKa = 3.13IPSSMWTTIGDD443 pKa = 3.83GALTISASTVDD454 pKa = 3.27AAGNTGEE461 pKa = 4.13ASRR464 pKa = 11.84DD465 pKa = 3.11ISIAAGLPGLRR476 pKa = 11.84VATVSGDD483 pKa = 3.92DD484 pKa = 3.61IINAIEE490 pKa = 4.05HH491 pKa = 6.16AQDD494 pKa = 3.26LTIFGTSTGLVAGQTVTATIDD515 pKa = 3.34GKK517 pKa = 11.43SYY519 pKa = 9.49LTAINADD526 pKa = 3.73GSWQVGLQASDD537 pKa = 3.12VSKK540 pKa = 11.18YY541 pKa = 7.43MQGLLVIHH549 pKa = 6.46VSSADD554 pKa = 3.29ASGNPVAIEE563 pKa = 3.85HH564 pKa = 6.29QIMVDD569 pKa = 3.6LADD572 pKa = 3.56TAVSINAVALDD583 pKa = 3.98NIINSVEE590 pKa = 4.02QASDD594 pKa = 3.72LLLSGATANVEE605 pKa = 3.87AGQVVVINFAGHH617 pKa = 5.81QYY619 pKa = 10.66LATVAGDD626 pKa = 3.92GGWSVVLPATDD637 pKa = 4.62LGGLTDD643 pKa = 4.54GYY645 pKa = 11.24RR646 pKa = 11.84NLEE649 pKa = 4.13VQVTNSTGSVAHH661 pKa = 6.46SEE663 pKa = 4.05QLVQVDD669 pKa = 4.02VTSPILVIGPAVSSGGLDD687 pKa = 3.44SAHH690 pKa = 7.68DD691 pKa = 4.14DD692 pKa = 3.83LLNALEE698 pKa = 4.31VNQDD702 pKa = 3.88LLVSGVTSAQVDD714 pKa = 3.57QALTVSLNGQSYY726 pKa = 6.56QTVVGGGGVWSVTIPSTNFSSAPDD750 pKa = 3.58GALLITATTTDD761 pKa = 3.21KK762 pKa = 11.38AGNSVLQTRR771 pKa = 11.84EE772 pKa = 3.98LTVDD776 pKa = 3.52TTAPVITINTVAGDD790 pKa = 3.71NVIDD794 pKa = 3.99GTEE797 pKa = 3.84QKK799 pKa = 10.6AGQLISGTADD809 pKa = 3.3TEE811 pKa = 4.21PGQVVEE817 pKa = 4.34VTFNGRR823 pKa = 11.84TYY825 pKa = 10.91QSVVADD831 pKa = 4.38GGAWSVDD838 pKa = 2.44VDD840 pKa = 4.15ARR842 pKa = 11.84DD843 pKa = 4.33FLDD846 pKa = 4.53TPDD849 pKa = 3.62GDD851 pKa = 4.27YY852 pKa = 10.99DD853 pKa = 3.48ITASVVDD860 pKa = 3.72KK861 pKa = 11.04AGNAGATNGSVYY873 pKa = 10.53LQADD877 pKa = 4.02APTVTINSFTADD889 pKa = 4.35DD890 pKa = 3.97ILSKK894 pKa = 10.94LEE896 pKa = 3.69QGAPQTLSGTSSVDD910 pKa = 2.68AGRR913 pKa = 11.84VVTVTLNGKK922 pKa = 9.78DD923 pKa = 3.36YY924 pKa = 10.61SAPVQGDD931 pKa = 3.73HH932 pKa = 6.26SWSVVLGTADD942 pKa = 3.87LAALADD948 pKa = 3.76SGVYY952 pKa = 10.38SITATATDD960 pKa = 3.63NVGNTGSSDD969 pKa = 3.07HH970 pKa = 6.78SFTVDD975 pKa = 3.31LSGPLMTLTIDD986 pKa = 4.45SITVDD991 pKa = 3.89SGPSSSDD998 pKa = 3.33FITNDD1003 pKa = 2.54TSLAVQGTLGAEE1015 pKa = 4.4LGAGEE1020 pKa = 4.97LMQISLDD1027 pKa = 4.99GGATWINLHH1036 pKa = 5.99VSGTTWTYY1044 pKa = 10.4VDD1046 pKa = 4.36SRR1048 pKa = 11.84TLGDD1052 pKa = 3.44GTHH1055 pKa = 7.6DD1056 pKa = 3.56YY1057 pKa = 10.07TVRR1060 pKa = 11.84LIDD1063 pKa = 3.4SLGNVGATASQLVTIDD1079 pKa = 3.52TNAPTSTIAITSISDD1094 pKa = 3.41DD1095 pKa = 3.33TGSYY1099 pKa = 10.35VDD1101 pKa = 5.29YY1102 pKa = 9.65ITNDD1106 pKa = 2.75TSLTVNGTLSSPLAVDD1122 pKa = 3.85EE1123 pKa = 4.59LAQISLDD1130 pKa = 4.44NGATWTALSIAGTTWSHH1147 pKa = 6.52ADD1149 pKa = 3.71DD1150 pKa = 3.75RR1151 pKa = 11.84TLVDD1155 pKa = 4.08GSYY1158 pKa = 10.36QYY1160 pKa = 9.55TVRR1163 pKa = 11.84VIDD1166 pKa = 3.53VAGSIGSTYY1175 pKa = 8.16TQTVIVDD1182 pKa = 3.89TAAPVAAIHH1191 pKa = 6.77IDD1193 pKa = 3.94SITDD1197 pKa = 3.44DD1198 pKa = 3.94TGVSATDD1205 pKa = 4.39YY1206 pKa = 9.43ITSDD1210 pKa = 2.84NTLVVNGTLSAALNTDD1226 pKa = 4.39EE1227 pKa = 4.93IAQISLDD1234 pKa = 3.74GGAVWTALVTSGLAWNYY1251 pKa = 9.46TDD1253 pKa = 5.81GRR1255 pKa = 11.84TLSDD1259 pKa = 3.52GVHH1262 pKa = 6.46TYY1264 pKa = 7.34QVRR1267 pKa = 11.84VVDD1270 pKa = 4.13LAGNSGPVEE1279 pKa = 4.09QQAVTVDD1286 pKa = 3.72RR1287 pKa = 11.84VAPTTSISIDD1297 pKa = 3.9SISDD1301 pKa = 3.45DD1302 pKa = 3.7TGLSLTDD1309 pKa = 4.57FITRR1313 pKa = 11.84DD1314 pKa = 3.27TSLTLSGTLGKK1325 pKa = 10.39ALEE1328 pKa = 4.36SDD1330 pKa = 3.78EE1331 pKa = 4.97LVQISIDD1338 pKa = 4.89AGTTWITLTDD1348 pKa = 3.44VTGTQWGYY1356 pKa = 9.95IDD1358 pKa = 5.46SRR1360 pKa = 11.84VLTDD1364 pKa = 4.0GNYY1367 pKa = 10.22DD1368 pKa = 3.43YY1369 pKa = 11.05QLRR1372 pKa = 11.84VIDD1375 pKa = 3.53QAGNIGQTASQTVVVDD1391 pKa = 4.87TIAPLEE1397 pKa = 3.93QATITGYY1404 pKa = 11.16DD1405 pKa = 3.61DD1406 pKa = 4.62DD1407 pKa = 3.86QGARR1411 pKa = 11.84QGIQTTHH1418 pKa = 7.02NITDD1422 pKa = 3.38DD1423 pKa = 3.85TAPVLQGTLTAEE1435 pKa = 4.19LQGGEE1440 pKa = 4.16VLKK1443 pKa = 10.69IFQNGIYY1450 pKa = 10.06LGVATVSGGTWAYY1463 pKa = 10.95ALTGLEE1469 pKa = 3.97NANTYY1474 pKa = 10.54DD1475 pKa = 3.55YY1476 pKa = 11.41SAVVTDD1482 pKa = 3.98AAGNTTTSADD1492 pKa = 3.19YY1493 pKa = 10.56RR1494 pKa = 11.84LNVDD1498 pKa = 3.4TSVPSSVTFEE1508 pKa = 4.14SQITSDD1514 pKa = 3.58TTPVLRR1520 pKa = 11.84GTILEE1525 pKa = 4.81DD1526 pKa = 3.98LSASQILYY1534 pKa = 8.49ITVNGITYY1542 pKa = 10.38SSSTSYY1548 pKa = 11.21GDD1550 pKa = 3.16GRR1552 pKa = 11.84VVVDD1556 pKa = 3.36TANRR1560 pKa = 11.84TWYY1563 pKa = 9.73VQIPSSDD1570 pKa = 3.29NLGIDD1575 pKa = 3.6TYY1577 pKa = 11.59DD1578 pKa = 3.65VIAKK1582 pKa = 8.79VVNEE1586 pKa = 4.58DD1587 pKa = 3.79SNGSLSSEE1595 pKa = 4.42TFSGAQVVIAEE1606 pKa = 4.39PAIDD1610 pKa = 3.95TQWAGDD1616 pKa = 3.75APGEE1620 pKa = 4.06RR1621 pKa = 11.84TNNQEE1626 pKa = 3.8GLSYY1630 pKa = 10.88SIGEE1634 pKa = 3.96NGLWNIVANTAVYY1647 pKa = 10.57NSSDD1651 pKa = 3.77LNTYY1655 pKa = 8.48STDD1658 pKa = 3.31VLAKK1662 pKa = 10.44EE1663 pKa = 4.44SVTGNTVNYY1672 pKa = 9.67TYY1674 pKa = 11.11FDD1676 pKa = 4.48LDD1678 pKa = 3.51RR1679 pKa = 11.84DD1680 pKa = 3.34GDD1682 pKa = 3.63MDD1684 pKa = 4.03IFATQTSYY1692 pKa = 10.45TRR1694 pKa = 11.84PFQYY1698 pKa = 9.16WQNDD1702 pKa = 3.23NGSYY1706 pKa = 8.71TSKK1709 pKa = 9.55TIEE1712 pKa = 3.95YY1713 pKa = 9.13GALDD1717 pKa = 4.09TYY1719 pKa = 11.6YY1720 pKa = 10.54GGTVAYY1726 pKa = 10.1DD1727 pKa = 3.41RR1728 pKa = 11.84TGNGYY1733 pKa = 10.49LDD1735 pKa = 4.56LVLGDD1740 pKa = 4.83SNLDD1744 pKa = 3.75SYY1746 pKa = 12.01SSGWFNNNQDD1756 pKa = 3.35GTFTQDD1762 pKa = 2.45TVTRR1766 pKa = 11.84GLTPSYY1772 pKa = 11.1SFGASISGVDD1782 pKa = 3.5INNDD1786 pKa = 2.97GTVDD1790 pKa = 2.87ITGQMNGGGGSNGYY1804 pKa = 7.26TLGVITNNGDD1814 pKa = 2.73GTMAIGQIVNNVFNDD1829 pKa = 3.36GGGADD1834 pKa = 3.65NVGTSMTWADD1844 pKa = 3.76FNGDD1848 pKa = 3.5GTLDD1852 pKa = 3.64LYY1854 pKa = 11.43LNQHH1858 pKa = 6.17FAGANKK1864 pKa = 10.09AAVLINNNGTLDD1876 pKa = 3.56NFVEE1880 pKa = 4.79VNAGPDD1886 pKa = 3.35VRR1888 pKa = 11.84SRR1890 pKa = 11.84IALAVDD1896 pKa = 3.34WDD1898 pKa = 4.35HH1899 pKa = 7.51NGTTDD1904 pKa = 2.99IAKK1907 pKa = 9.58MGGYY1911 pKa = 10.45NNGDD1915 pKa = 4.15TIQMILNNGNGLSWTNANLNYY1936 pKa = 10.21SYY1938 pKa = 11.32SSRR1941 pKa = 11.84LTGAAAMDD1949 pKa = 4.32YY1950 pKa = 10.47DD1951 pKa = 3.47WDD1953 pKa = 4.14GAVDD1957 pKa = 6.23LIGFTNNGHH1966 pKa = 5.55SFVVKK1971 pKa = 9.17NTNAVAEE1978 pKa = 4.62GTSMHH1983 pKa = 6.49LRR1985 pKa = 11.84IVDD1988 pKa = 3.55QNGINAFYY1996 pKa = 10.85GNTVQLFDD2004 pKa = 3.75SSGALVATQILNAQSGVGVNDD2025 pKa = 3.59SSALLNFYY2033 pKa = 10.44GLSASEE2039 pKa = 4.39TYY2041 pKa = 10.42SVNLIRR2047 pKa = 11.84IVDD2050 pKa = 4.13GVRR2053 pKa = 11.84SDD2055 pKa = 3.35VTEE2058 pKa = 4.2TVNSGWGGLTTGDD2071 pKa = 3.24ATHH2074 pKa = 6.83NYY2076 pKa = 8.11VLSVEE2081 pKa = 4.33SDD2083 pKa = 2.96SDD2085 pKa = 3.7RR2086 pKa = 11.84NNGVFVGSGYY2096 pKa = 10.79NDD2098 pKa = 3.43TFIATAGSDD2107 pKa = 3.58TYY2109 pKa = 11.45NGGGGWSYY2117 pKa = 11.43SSEE2120 pKa = 3.92HH2121 pKa = 5.69GTWVAANGGMDD2132 pKa = 3.39VVDD2135 pKa = 3.84YY2136 pKa = 11.09QLATNGVTVDD2146 pKa = 4.2LSSTATQDD2154 pKa = 2.98TGFNSAKK2161 pKa = 10.27LVDD2164 pKa = 3.88VEE2166 pKa = 4.75GVLGSSFDD2174 pKa = 5.04DD2175 pKa = 3.52IFTGSAGDD2183 pKa = 3.46NQFQGRR2189 pKa = 11.84GGNDD2193 pKa = 4.55LINIANGGHH2202 pKa = 7.34DD2203 pKa = 3.59SLIYY2207 pKa = 10.56NLLSAVDD2214 pKa = 3.66ATGGNGTDD2222 pKa = 4.08AIAGFTVGTWEE2233 pKa = 4.22GTNDD2237 pKa = 3.51TDD2239 pKa = 5.73RR2240 pKa = 11.84IDD2242 pKa = 3.59INEE2245 pKa = 4.35LLHH2248 pKa = 5.8SAGYY2252 pKa = 8.43TGTGSASYY2260 pKa = 10.91VDD2262 pKa = 5.02GVATLDD2268 pKa = 3.48TSAGNIEE2275 pKa = 5.17DD2276 pKa = 4.07YY2277 pKa = 11.35LSVLQNGANTDD2288 pKa = 3.05IQFDD2292 pKa = 3.82HH2293 pKa = 7.59DD2294 pKa = 4.24GLGGAFDD2301 pKa = 4.03PTTIASLSGVQVDD2314 pKa = 5.43LATLLANHH2322 pKa = 6.16QLLVAA2327 pKa = 5.06

Molecular weight:
241.01 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1Y0KMC8|A0A1Y0KMC8_9PSED Pyrroline-5-carboxylate reductase OS=Pseudomonas sp. M30-35 OX=1981174 GN=proC PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.38GRR39 pKa = 11.84ARR41 pKa = 11.84LAVV44 pKa = 3.42

Molecular weight:
5.07 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4347

0

4347

1427656

38

4652

328.4

36.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.64 ± 0.041

1.023 ± 0.013

5.371 ± 0.042

5.751 ± 0.039

3.716 ± 0.028

7.719 ± 0.047

2.138 ± 0.018

5.221 ± 0.029

3.75 ± 0.033

11.582 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.419 ± 0.021

3.326 ± 0.028

4.625 ± 0.026

4.679 ± 0.033

5.831 ± 0.033

6.24 ± 0.032

4.951 ± 0.038

7.052 ± 0.032

1.388 ± 0.016

2.58 ± 0.018

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski