Amino acid dipepetide frequency for Pseudomonas sp. M30-35

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.691AlaAla: 11.691 ± 0.136
1.246AlaCys: 1.246 ± 0.031
5.824AlaAsp: 5.824 ± 0.073
6.932AlaGlu: 6.932 ± 0.079
3.704AlaPhe: 3.704 ± 0.054
8.477AlaGly: 8.477 ± 0.095
2.076AlaHis: 2.076 ± 0.04
5.426AlaIle: 5.426 ± 0.074
4.208AlaLys: 4.208 ± 0.067
12.877AlaLeu: 12.877 ± 0.114
2.866AlaMet: 2.866 ± 0.049
3.242AlaAsn: 3.242 ± 0.051
4.255AlaPro: 4.255 ± 0.06
5.232AlaGln: 5.232 ± 0.077
5.916AlaArg: 5.916 ± 0.061
6.215AlaSer: 6.215 ± 0.066
4.914AlaThr: 4.914 ± 0.068
7.346AlaVal: 7.346 ± 0.089
1.454AlaTrp: 1.454 ± 0.037
2.503AlaTyr: 2.503 ± 0.038
0.0AlaXaa: 0.0 ± 0.0
Cys
1.137CysAla: 1.137 ± 0.029
0.152CysCys: 0.152 ± 0.012
0.54CysAsp: 0.54 ± 0.022
0.55CysGlu: 0.55 ± 0.023
0.361CysPhe: 0.361 ± 0.017
0.929CysGly: 0.929 ± 0.03
0.275CysHis: 0.275 ± 0.015
0.512CysIle: 0.512 ± 0.017
0.326CysLys: 0.326 ± 0.016
1.083CysLeu: 1.083 ± 0.032
0.251CysMet: 0.251 ± 0.016
0.302CysAsn: 0.302 ± 0.014
0.5CysPro: 0.5 ± 0.02
0.392CysGln: 0.392 ± 0.02
0.544CysArg: 0.544 ± 0.02
0.712CysSer: 0.712 ± 0.026
0.515CysThr: 0.515 ± 0.019
0.734CysVal: 0.734 ± 0.026
0.153CysTrp: 0.153 ± 0.01
0.257CysTyr: 0.257 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
5.529AspAla: 5.529 ± 0.066
0.539AspCys: 0.539 ± 0.018
3.003AspAsp: 3.003 ± 0.06
3.392AspGlu: 3.392 ± 0.051
2.115AspPhe: 2.115 ± 0.038
4.268AspGly: 4.268 ± 0.075
1.054AspHis: 1.054 ± 0.026
2.981AspIle: 2.981 ± 0.048
2.153AspLys: 2.153 ± 0.049
5.757AspLeu: 5.757 ± 0.071
1.264AspMet: 1.264 ± 0.03
1.846AspAsn: 1.846 ± 0.043
2.578AspPro: 2.578 ± 0.046
2.309AspGln: 2.309 ± 0.047
2.761AspArg: 2.761 ± 0.047
3.247AspSer: 3.247 ± 0.048
2.513AspThr: 2.513 ± 0.059
3.698AspVal: 3.698 ± 0.056
0.95AspTrp: 0.95 ± 0.027
1.751AspTyr: 1.751 ± 0.034
0.0AspXaa: 0.0 ± 0.0
Glu
5.966GluAla: 5.966 ± 0.074
0.423GluCys: 0.423 ± 0.015
2.542GluAsp: 2.542 ± 0.042
2.956GluGlu: 2.956 ± 0.063
1.961GluPhe: 1.961 ± 0.042
3.768GluGly: 3.768 ± 0.06
1.665GluHis: 1.665 ± 0.037
2.874GluIle: 2.874 ± 0.05
2.22GluLys: 2.22 ± 0.041
7.194GluLeu: 7.194 ± 0.085
1.376GluMet: 1.376 ± 0.033
1.751GluAsn: 1.751 ± 0.04
2.51GluPro: 2.51 ± 0.047
4.083GluGln: 4.083 ± 0.063
4.383GluArg: 4.383 ± 0.068
2.979GluSer: 2.979 ± 0.047
2.707GluThr: 2.707 ± 0.045
4.163GluVal: 4.163 ± 0.061
0.697GluTrp: 0.697 ± 0.023
1.291GluTyr: 1.291 ± 0.028
0.0GluXaa: 0.0 ± 0.0
Phe
4.227PheAla: 4.227 ± 0.06
0.464PheCys: 0.464 ± 0.018
2.449PheAsp: 2.449 ± 0.044
2.151PheGlu: 2.151 ± 0.042
1.479PhePhe: 1.479 ± 0.038
3.123PheGly: 3.123 ± 0.06
0.677PheHis: 0.677 ± 0.021
2.123PheIle: 2.123 ± 0.045
1.439PheLys: 1.439 ± 0.034
3.203PheLeu: 3.203 ± 0.052
0.908PheMet: 0.908 ± 0.028
1.523PheAsn: 1.523 ± 0.032
1.402PhePro: 1.402 ± 0.032
1.188PheGln: 1.188 ± 0.025
1.675PheArg: 1.675 ± 0.038
2.861PheSer: 2.861 ± 0.046
2.054PheThr: 2.054 ± 0.047
2.531PheVal: 2.531 ± 0.046
0.563PheTrp: 0.563 ± 0.02
1.116PheTyr: 1.116 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
7.15GlyAla: 7.15 ± 0.093
0.972GlyCys: 0.972 ± 0.03
3.918GlyAsp: 3.918 ± 0.077
4.472GlyGlu: 4.472 ± 0.06
3.314GlyPhe: 3.314 ± 0.051
5.96GlyGly: 5.96 ± 0.157
1.673GlyHis: 1.673 ± 0.036
4.421GlyIle: 4.421 ± 0.062
3.455GlyLys: 3.455 ± 0.044
9.012GlyLeu: 9.012 ± 0.087
2.191GlyMet: 2.191 ± 0.042
2.521GlyAsn: 2.521 ± 0.068
2.417GlyPro: 2.417 ± 0.042
3.373GlyGln: 3.373 ± 0.063
4.217GlyArg: 4.217 ± 0.06
4.668GlySer: 4.668 ± 0.076
3.771GlyThr: 3.771 ± 0.068
5.957GlyVal: 5.957 ± 0.078
1.251GlyTrp: 1.251 ± 0.037
2.473GlyTyr: 2.473 ± 0.049
0.0GlyXaa: 0.0 ± 0.0
His
2.124HisAla: 2.124 ± 0.043
0.307HisCys: 0.307 ± 0.015
1.087HisAsp: 1.087 ± 0.029
1.114HisGlu: 1.114 ± 0.029
0.931HisPhe: 0.931 ± 0.029
1.713HisGly: 1.713 ± 0.039
0.549HisHis: 0.549 ± 0.023
1.119HisIle: 1.119 ± 0.025
0.738HisLys: 0.738 ± 0.02
2.364HisLeu: 2.364 ± 0.05
0.523HisMet: 0.523 ± 0.021
0.735HisAsn: 0.735 ± 0.025
1.284HisPro: 1.284 ± 0.037
0.981HisGln: 0.981 ± 0.026
1.152HisArg: 1.152 ± 0.031
1.309HisSer: 1.309 ± 0.033
0.934HisThr: 0.934 ± 0.028
1.331HisVal: 1.331 ± 0.033
0.401HisTrp: 0.401 ± 0.014
0.687HisTyr: 0.687 ± 0.024
0.0HisXaa: 0.0 ± 0.0
Ile
6.281IleAla: 6.281 ± 0.076
0.604IleCys: 0.604 ± 0.02
3.58IleAsp: 3.58 ± 0.052
3.715IleGlu: 3.715 ± 0.06
1.705IlePhe: 1.705 ± 0.037
4.763IleGly: 4.763 ± 0.069
0.939IleHis: 0.939 ± 0.028
2.506IleIle: 2.506 ± 0.052
2.062IleLys: 2.062 ± 0.039
4.436IleLeu: 4.436 ± 0.06
0.951IleMet: 0.951 ± 0.027
2.122IleAsn: 2.122 ± 0.039
2.332IlePro: 2.332 ± 0.044
1.711IleGln: 1.711 ± 0.036
2.775IleArg: 2.775 ± 0.053
3.635IleSer: 3.635 ± 0.055
2.903IleThr: 2.903 ± 0.054
3.381IleVal: 3.381 ± 0.053
0.547IleTrp: 0.547 ± 0.021
1.267IleTyr: 1.267 ± 0.028
0.0IleXaa: 0.0 ± 0.0
Lys
4.236LysAla: 4.236 ± 0.062
0.198LysCys: 0.198 ± 0.012
1.85LysAsp: 1.85 ± 0.042
1.811LysGlu: 1.811 ± 0.038
1.049LysPhe: 1.049 ± 0.028
2.674LysGly: 2.674 ± 0.055
0.851LysHis: 0.851 ± 0.024
1.884LysIle: 1.884 ± 0.039
1.447LysLys: 1.447 ± 0.038
4.313LysLeu: 4.313 ± 0.068
0.85LysMet: 0.85 ± 0.025
1.194LysAsn: 1.194 ± 0.026
2.255LysPro: 2.255 ± 0.048
1.992LysGln: 1.992 ± 0.038
2.554LysArg: 2.554 ± 0.047
2.136LysSer: 2.136 ± 0.038
2.131LysThr: 2.131 ± 0.039
2.886LysVal: 2.886 ± 0.047
0.405LysTrp: 0.405 ± 0.016
0.787LysTyr: 0.787 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
13.203LeuAla: 13.203 ± 0.117
1.21LeuCys: 1.21 ± 0.03
6.474LeuAsp: 6.474 ± 0.078
6.433LeuGlu: 6.433 ± 0.075
4.16LeuPhe: 4.16 ± 0.059
8.705LeuGly: 8.705 ± 0.089
2.323LeuHis: 2.323 ± 0.047
6.08LeuIle: 6.08 ± 0.074
4.652LeuLys: 4.652 ± 0.061
13.152LeuLeu: 13.152 ± 0.14
2.661LeuMet: 2.661 ± 0.051
4.192LeuAsn: 4.192 ± 0.051
6.015LeuPro: 6.015 ± 0.081
4.802LeuGln: 4.802 ± 0.071
6.568LeuArg: 6.568 ± 0.074
7.95LeuSer: 7.95 ± 0.082
5.826LeuThr: 5.826 ± 0.068
7.601LeuVal: 7.601 ± 0.102
1.308LeuTrp: 1.308 ± 0.036
2.506LeuTyr: 2.506 ± 0.04
0.0LeuXaa: 0.0 ± 0.0
Met
2.739MetAla: 2.739 ± 0.045
0.195MetCys: 0.195 ± 0.013
1.029MetAsp: 1.029 ± 0.028
0.913MetGlu: 0.913 ± 0.027
0.763MetPhe: 0.763 ± 0.024
1.701MetGly: 1.701 ± 0.038
0.536MetHis: 0.536 ± 0.018
1.273MetIle: 1.273 ± 0.032
0.879MetLys: 0.879 ± 0.026
2.952MetLeu: 2.952 ± 0.046
0.544MetMet: 0.544 ± 0.023
0.881MetAsn: 0.881 ± 0.025
1.422MetPro: 1.422 ± 0.035
1.215MetGln: 1.215 ± 0.032
1.598MetArg: 1.598 ± 0.035
1.869MetSer: 1.869 ± 0.037
1.474MetThr: 1.474 ± 0.035
1.618MetVal: 1.618 ± 0.035
0.202MetTrp: 0.202 ± 0.013
0.387MetTyr: 0.387 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
3.325AsnAla: 3.325 ± 0.052
0.361AsnCys: 0.361 ± 0.015
1.814AsnAsp: 1.814 ± 0.052
1.629AsnGlu: 1.629 ± 0.037
1.234AsnPhe: 1.234 ± 0.03
2.692AsnGly: 2.692 ± 0.056
0.656AsnHis: 0.656 ± 0.02
1.786AsnIle: 1.786 ± 0.034
1.194AsnLys: 1.194 ± 0.035
3.681AsnLeu: 3.681 ± 0.052
0.743AsnMet: 0.743 ± 0.021
1.214AsnAsn: 1.214 ± 0.038
2.092AsnPro: 2.092 ± 0.044
1.577AsnGln: 1.577 ± 0.032
1.856AsnArg: 1.856 ± 0.041
2.105AsnSer: 2.105 ± 0.045
1.669AsnThr: 1.669 ± 0.036
2.129AsnVal: 2.129 ± 0.043
0.537AsnTrp: 0.537 ± 0.023
0.96AsnTyr: 0.96 ± 0.032
0.0AsnXaa: 0.0 ± 0.0
Pro
5.12ProAla: 5.12 ± 0.073
0.361ProCys: 0.361 ± 0.017
2.763ProAsp: 2.763 ± 0.048
3.367ProGlu: 3.367 ± 0.052
1.764ProPhe: 1.764 ± 0.037
3.505ProGly: 3.505 ± 0.047
0.979ProHis: 0.979 ± 0.03
2.215ProIle: 2.215 ± 0.038
1.693ProLys: 1.693 ± 0.036
5.326ProLeu: 5.326 ± 0.078
1.134ProMet: 1.134 ± 0.029
1.501ProAsn: 1.501 ± 0.028
1.643ProPro: 1.643 ± 0.037
2.2ProGln: 2.2 ± 0.048
2.258ProArg: 2.258 ± 0.05
2.707ProSer: 2.707 ± 0.045
2.221ProThr: 2.221 ± 0.04
3.665ProVal: 3.665 ± 0.057
0.685ProTrp: 0.685 ± 0.025
1.138ProTyr: 1.138 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
5.59GlnAla: 5.59 ± 0.074
0.336GlnCys: 0.336 ± 0.017
1.835GlnAsp: 1.835 ± 0.039
1.949GlnGlu: 1.949 ± 0.046
1.498GlnPhe: 1.498 ± 0.035
3.387GlnGly: 3.387 ± 0.05
1.187GlnHis: 1.187 ± 0.034
2.287GlnIle: 2.287 ± 0.046
1.348GlnLys: 1.348 ± 0.032
6.025GlnLeu: 6.025 ± 0.088
1.149GlnMet: 1.149 ± 0.027
1.189GlnAsn: 1.189 ± 0.03
2.429GlnPro: 2.429 ± 0.046
3.03GlnGln: 3.03 ± 0.073
3.556GlnArg: 3.556 ± 0.063
2.593GlnSer: 2.593 ± 0.049
2.136GlnThr: 2.136 ± 0.042
3.539GlnVal: 3.539 ± 0.06
0.692GlnTrp: 0.692 ± 0.023
1.029GlnTyr: 1.029 ± 0.028
0.0GlnXaa: 0.0 ± 0.0
Arg
5.284ArgAla: 5.284 ± 0.069
0.559ArgCys: 0.559 ± 0.021
3.125ArgAsp: 3.125 ± 0.042
3.915ArgGlu: 3.915 ± 0.055
2.517ArgPhe: 2.517 ± 0.044
3.641ArgGly: 3.641 ± 0.055
1.391ArgHis: 1.391 ± 0.037
3.207ArgIle: 3.207 ± 0.048
2.204ArgLys: 2.204 ± 0.04
7.333ArgLeu: 7.333 ± 0.092
1.553ArgMet: 1.553 ± 0.032
1.978ArgAsn: 1.978 ± 0.041
2.349ArgPro: 2.349 ± 0.04
2.934ArgGln: 2.934 ± 0.05
3.481ArgArg: 3.481 ± 0.062
3.317ArgSer: 3.317 ± 0.058
2.523ArgThr: 2.523 ± 0.04
4.107ArgVal: 4.107 ± 0.059
0.929ArgTrp: 0.929 ± 0.025
1.965ArgTyr: 1.965 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
6.399SerAla: 6.399 ± 0.067
0.574SerCys: 0.574 ± 0.022
3.172SerAsp: 3.172 ± 0.054
3.422SerGlu: 3.422 ± 0.047
2.432SerPhe: 2.432 ± 0.041
5.504SerGly: 5.504 ± 0.078
1.308SerHis: 1.308 ± 0.037
3.303SerIle: 3.303 ± 0.051
2.291SerLys: 2.291 ± 0.044
7.353SerLeu: 7.353 ± 0.085
1.566SerMet: 1.566 ± 0.03
2.108SerAsn: 2.108 ± 0.043
2.693SerPro: 2.693 ± 0.042
2.66SerGln: 2.66 ± 0.045
3.562SerArg: 3.562 ± 0.049
4.068SerSer: 4.068 ± 0.06
3.116SerThr: 3.116 ± 0.047
4.31SerVal: 4.31 ± 0.066
0.901SerTrp: 0.901 ± 0.026
1.655SerTyr: 1.655 ± 0.035
0.0SerXaa: 0.0 ± 0.0
Thr
5.254ThrAla: 5.254 ± 0.071
0.495ThrCys: 0.495 ± 0.019
2.677ThrAsp: 2.677 ± 0.05
2.697ThrGlu: 2.697 ± 0.045
1.856ThrPhe: 1.856 ± 0.035
4.238ThrGly: 4.238 ± 0.061
1.03ThrHis: 1.03 ± 0.027
2.306ThrIle: 2.306 ± 0.052
1.262ThrLys: 1.262 ± 0.034
6.511ThrLeu: 6.511 ± 0.071
0.877ThrMet: 0.877 ± 0.028
1.399ThrAsn: 1.399 ± 0.037
3.057ThrPro: 3.057 ± 0.051
2.193ThrGln: 2.193 ± 0.038
2.731ThrArg: 2.731 ± 0.042
2.894ThrSer: 2.894 ± 0.049
2.54ThrThr: 2.54 ± 0.049
3.542ThrVal: 3.542 ± 0.07
0.668ThrTrp: 0.668 ± 0.021
1.281ThrTyr: 1.281 ± 0.033
0.0ThrXaa: 0.0 ± 0.0
Val
7.532ValAla: 7.532 ± 0.095
0.762ValCys: 0.762 ± 0.025
4.039ValAsp: 4.039 ± 0.054
4.374ValGlu: 4.374 ± 0.061
2.703ValPhe: 2.703 ± 0.052
5.199ValGly: 5.199 ± 0.072
1.315ValHis: 1.315 ± 0.033
4.113ValIle: 4.113 ± 0.062
2.574ValLys: 2.574 ± 0.047
8.01ValLeu: 8.01 ± 0.091
1.868ValMet: 1.868 ± 0.037
2.328ValAsn: 2.328 ± 0.05
3.08ValPro: 3.08 ± 0.047
2.545ValGln: 2.545 ± 0.041
3.948ValArg: 3.948 ± 0.053
4.479ValSer: 4.479 ± 0.06
3.685ValThr: 3.685 ± 0.062
5.428ValVal: 5.428 ± 0.07
0.899ValTrp: 0.899 ± 0.023
1.638ValTyr: 1.638 ± 0.036
0.0ValXaa: 0.0 ± 0.0
Trp
1.141TrpAla: 1.141 ± 0.032
0.16TrpCys: 0.16 ± 0.01
0.606TrpAsp: 0.606 ± 0.023
0.532TrpGlu: 0.532 ± 0.02
0.548TrpPhe: 0.548 ± 0.021
0.867TrpGly: 0.867 ± 0.026
0.34TrpHis: 0.34 ± 0.015
0.663TrpIle: 0.663 ± 0.026
0.45TrpLys: 0.45 ± 0.018
2.178TrpLeu: 2.178 ± 0.052
0.393TrpMet: 0.393 ± 0.018
0.436TrpAsn: 0.436 ± 0.017
0.63TrpPro: 0.63 ± 0.026
0.995TrpGln: 0.995 ± 0.027
0.989TrpArg: 0.989 ± 0.031
0.848TrpSer: 0.848 ± 0.028
0.6TrpThr: 0.6 ± 0.026
0.939TrpVal: 0.939 ± 0.028
0.219TrpTrp: 0.219 ± 0.012
0.341TrpTyr: 0.341 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.475TyrAla: 2.475 ± 0.036
0.307TyrCys: 0.307 ± 0.015
1.382TyrAsp: 1.382 ± 0.033
1.184TyrGlu: 1.184 ± 0.029
1.063TyrPhe: 1.063 ± 0.029
2.072TyrGly: 2.072 ± 0.045
0.559TyrHis: 0.559 ± 0.02
1.135TyrIle: 1.135 ± 0.029
0.906TyrLys: 0.906 ± 0.032
3.058TyrLeu: 3.058 ± 0.048
0.522TyrMet: 0.522 ± 0.019
0.794TyrAsn: 0.794 ± 0.024
1.303TyrPro: 1.303 ± 0.033
1.373TyrGln: 1.373 ± 0.034
1.789TyrArg: 1.789 ± 0.036
1.808TyrSer: 1.808 ± 0.041
1.276TyrThr: 1.276 ± 0.035
1.613TyrVal: 1.613 ± 0.036
0.414TyrTrp: 0.414 ± 0.019
0.766TyrTyr: 0.766 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4347 proteins (1427656 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski