Marinobacter persicus
Average proteome isoelectric point is 6.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2913 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I3UGW0|A0A1I3UGW0_9ALTE Secondary thiamine-phosphate synthase enzyme OS=Marinobacter persicus OX=930118 GN=SAMN05216429_106106 PE=3 SV=1
MM1 pKa = 7.39 SSSGGGDD8 pKa = 3.1 INIGGGSGLYY18 pKa = 9.35 TVDD21 pKa = 3.0 GCAYY25 pKa = 10.37 SCGSNPAKK33 pKa = 9.88 WDD35 pKa = 3.51 GNHH38 pKa = 6.93 TYY40 pKa = 10.79 GQTLEE45 pKa = 4.22 CKK47 pKa = 10.2 GLGVTPEE54 pKa = 3.71 EE55 pKa = 4.59 AEE57 pKa = 4.07 AGEE60 pKa = 4.98 AGPADD65 pKa = 4.63 LNCATDD71 pKa = 3.36 SSGKK75 pKa = 9.55 EE76 pKa = 3.68 ICSDD80 pKa = 3.91 PDD82 pKa = 3.35 NPSCVTIDD90 pKa = 4.92 GISTCPSEE98 pKa = 5.34 GAICDD103 pKa = 4.19 EE104 pKa = 4.49 LNGDD108 pKa = 5.04 LGCIDD113 pKa = 4.16 PAEE116 pKa = 4.41 EE117 pKa = 4.3 GCAYY121 pKa = 10.78 RR122 pKa = 11.84 NGKK125 pKa = 8.47 KK126 pKa = 9.45 EE127 pKa = 4.02 CYY129 pKa = 9.9 DD130 pKa = 3.03 IFGQYY135 pKa = 9.11 IEE137 pKa = 5.38 HH138 pKa = 7.9 DD139 pKa = 4.4 SPDD142 pKa = 3.48 HH143 pKa = 6.48 PNNGGNMDD151 pKa = 4.32 GDD153 pKa = 4.19 TSNDD157 pKa = 3.21 MTDD160 pKa = 3.5 PRR162 pKa = 11.84 PEE164 pKa = 4.68 GEE166 pKa = 4.99 GGDD169 pKa = 4.4 PNNQPGDD176 pKa = 3.75 STGEE180 pKa = 3.97 GGEE183 pKa = 4.19 ATEE186 pKa = 4.48 GTAKK190 pKa = 10.47 EE191 pKa = 4.16 SLEE194 pKa = 3.81 QQKK197 pKa = 10.34 IGNEE201 pKa = 3.87 KK202 pKa = 10.37 LDD204 pKa = 4.59 GIEE207 pKa = 4.15 EE208 pKa = 4.35 STGNIDD214 pKa = 3.65 EE215 pKa = 5.44 AIEE218 pKa = 4.13 AALDD222 pKa = 3.47 ASRR225 pKa = 11.84 GDD227 pKa = 3.49 GTALKK232 pKa = 10.72 NGIDD236 pKa = 3.98 DD237 pKa = 5.57 AISNAGDD244 pKa = 3.78 SAVGDD249 pKa = 4.1 LDD251 pKa = 3.63 EE252 pKa = 6.69 HH253 pKa = 8.03 IDD255 pKa = 4.9 GIDD258 pKa = 3.32 SPAPMNEE265 pKa = 3.68 GDD267 pKa = 5.11 LGAIEE272 pKa = 5.0 NNVTNLFAGSMQCRR286 pKa = 11.84 DD287 pKa = 3.58 LVFGTDD293 pKa = 3.53 EE294 pKa = 3.55 ISYY297 pKa = 9.25 TITCNDD303 pKa = 2.87 MSMIRR308 pKa = 11.84 DD309 pKa = 3.42 MLGFLLYY316 pKa = 10.39 GFTVIRR322 pKa = 11.84 LFNVALRR329 pKa = 11.84 PAAKK333 pKa = 8.27 GTPP336 pKa = 3.43
Molecular weight: 34.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.712
IPC2_protein 3.745
IPC_protein 3.77
Toseland 3.554
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.681
Rodwell 3.592
Grimsley 3.452
Solomon 3.745
Lehninger 3.706
Nozaki 3.859
DTASelect 4.088
Thurlkill 3.592
EMBOSS 3.681
Sillero 3.884
Patrickios 0.858
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.799
Protein with the highest isoelectric point:
>tr|A0A1I3V6M6|A0A1I3V6M6_9ALTE RecBCD enzyme subunit RecD OS=Marinobacter persicus OX=930118 GN=recD PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.42 RR12 pKa = 11.84 KK13 pKa = 9.04 RR14 pKa = 11.84 AHH16 pKa = 6.12 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATANGRR28 pKa = 11.84 KK29 pKa = 8.91 VLSRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.44 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2913
0
2913
959929
29
5842
329.5
36.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.819 ± 0.05
0.902 ± 0.015
5.87 ± 0.045
6.904 ± 0.05
3.687 ± 0.029
7.78 ± 0.061
2.26 ± 0.025
5.124 ± 0.035
3.758 ± 0.043
10.704 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.556 ± 0.021
3.293 ± 0.037
4.673 ± 0.036
4.246 ± 0.033
6.524 ± 0.039
5.647 ± 0.038
5.132 ± 0.035
7.256 ± 0.041
1.357 ± 0.017
2.509 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here