Paraburkholderia sp. PDC91

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia; unclassified Paraburkholderia

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6876 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2W7FHD9|A0A2W7FHD9_9BURK Amino acid ABC transporter substrate-binding protein (PAAT family) OS=Paraburkholderia sp. PDC91 OX=2135498 GN=C7514_116141 PE=4 SV=1
MM1 pKa = 7.36NKK3 pKa = 9.75RR4 pKa = 11.84YY5 pKa = 9.72RR6 pKa = 11.84KK7 pKa = 9.32QPMRR11 pKa = 11.84TRR13 pKa = 11.84TIASVVAALFEE24 pKa = 4.26ASYY27 pKa = 11.27ASSLLANPTGAQVVAGSVNVNSPTAGQMNITQGTQNAIVNWNTFSIGAGEE77 pKa = 4.68SVTVAQPSASAALLNRR93 pKa = 11.84VVGNDD98 pKa = 3.18PSTIAGRR105 pKa = 11.84LQANGKK111 pKa = 9.22VFLVNPAGVIFSPGSSVNVGSLVASTLNISDD142 pKa = 4.24ADD144 pKa = 3.86FLAGNYY150 pKa = 9.46HH151 pKa = 6.54FVGASPAGVSNAGTLAAQQGGTIALLGGTVSNSGTVTAKK190 pKa = 10.71LGTVALGAGSDD201 pKa = 3.33ISLDD205 pKa = 3.66FAGDD209 pKa = 3.44GLTTLTINSAAAQALVGNTGTLAADD234 pKa = 3.98GGLVVMSAQTADD246 pKa = 3.49ALASSVVNQQGIVRR260 pKa = 11.84AQSLAEE266 pKa = 4.06RR267 pKa = 11.84NGHH270 pKa = 5.83IVLDD274 pKa = 4.29GGSNGTTTVSGTLDD288 pKa = 3.26ATGGAGLSGGRR299 pKa = 11.84IDD301 pKa = 3.52ATGRR305 pKa = 11.84DD306 pKa = 3.62VALLAGANVDD316 pKa = 3.25ASGAAGGGTVNLGGGAGGQDD336 pKa = 3.31PAIRR340 pKa = 11.84NADD343 pKa = 3.82TLSIDD348 pKa = 3.71PAAQVHH354 pKa = 6.79ADD356 pKa = 3.38ALTNGNGGSVSAYY369 pKa = 10.41SDD371 pKa = 3.45TSGLLYY377 pKa = 9.52GTLTAKK383 pKa = 10.57GGPQGGNGGMVEE395 pKa = 4.32TSSHH399 pKa = 5.69YY400 pKa = 10.98LDD402 pKa = 5.17LLGSTIDD409 pKa = 3.53VSAPAGNGGMWKK421 pKa = 9.47IDD423 pKa = 3.87PYY425 pKa = 11.54EE426 pKa = 4.03MNITDD431 pKa = 4.33NPNEE435 pKa = 4.15TVPTGGSLVLASEE448 pKa = 4.96IEE450 pKa = 3.9QSLNQGARR458 pKa = 11.84VIVDD462 pKa = 3.85AASTDD467 pKa = 3.51PGSINVEE474 pKa = 4.3SPISMTGARR483 pKa = 11.84PASLTLMASGSISVEE498 pKa = 3.63PNANITSTGGALDD511 pKa = 3.78LTLNAAIGADD521 pKa = 3.62PTAGSVSINAATIDD535 pKa = 3.9TNGGAFTINAANSGGQSPVSIMDD558 pKa = 3.52STISTGAGAITINGNATTGGAIALGYY584 pKa = 7.93NTLLATTTGNVTLTGVAQQGDD605 pKa = 4.0GVDD608 pKa = 3.6IEE610 pKa = 4.61SSAVQTANGAIQISGAGTAFEE631 pKa = 3.99PTAYY635 pKa = 9.82GVYY638 pKa = 9.29ATGGYY643 pKa = 7.86TPAGSDD649 pKa = 3.35TASVPVLGATGTGSISVTGTATGNSATGVEE679 pKa = 4.28LDD681 pKa = 3.63GATVSAASGGVTINGTANAANVGNGVSLSDD711 pKa = 3.78AAIDD715 pKa = 3.71TASGNVSITGTATNQQVAVGYY736 pKa = 10.46ALSTTANGIALGYY749 pKa = 10.26SSVASIDD756 pKa = 3.25GDD758 pKa = 3.86LALNGSTQVMGDD770 pKa = 3.75GVRR773 pKa = 11.84MLQATLQTTGSGSIAVTGNANVPDD797 pKa = 4.01STALQTGVDD806 pKa = 3.81VAGTTVSASGTGSIALTGNANGEE829 pKa = 4.23SSDD832 pKa = 3.63GVVVIDD838 pKa = 3.82ATILGAAGAISVNGSATATANGGNAAGVAVQEE870 pKa = 4.43SALNSTTGAINVTGAALAGADD891 pKa = 3.43GLTYY895 pKa = 10.79SGDD898 pKa = 3.75GVTMYY903 pKa = 10.21YY904 pKa = 11.04GSIGTTSGNISVTGTATGANSGASFSNADD933 pKa = 3.2GVAVQSSYY941 pKa = 10.92GQQSAGTAGMAFSSTAGGSIDD962 pKa = 4.59IEE964 pKa = 4.66GSAQGQYY971 pKa = 10.95VIGVGLDD978 pKa = 3.28SAGISATTGPITVHH992 pKa = 6.35GSADD996 pKa = 3.57TTISGEE1002 pKa = 4.5GVDD1005 pKa = 3.96TDD1007 pKa = 3.49IALLSSTSGAITVNGSANASDD1028 pKa = 3.85AGTGRR1033 pKa = 11.84GVEE1036 pKa = 4.04LGYY1039 pKa = 9.68TSLTTTSGGVNVTGTVTGTPGQATGATIGAGYY1071 pKa = 8.27TPQQEE1076 pKa = 4.58EE1077 pKa = 4.13EE1078 pKa = 4.36GGPLTEE1084 pKa = 5.37AVAGDD1089 pKa = 4.19PPSVQTDD1096 pKa = 3.27SGNITIAGSAPATAQGSNALSLFSASIVSNSGAIGLTGTTTGTPTPGTSSTGVLLNGSNGDD1157 pKa = 3.45GFTNTSITTGSGALTIEE1174 pKa = 4.78GSGSGQLAAGVAIANATTITSTSGGAIDD1202 pKa = 3.64IRR1204 pKa = 11.84GVATSPTTSTAGGNIQYY1221 pKa = 10.7DD1222 pKa = 4.19YY1223 pKa = 10.25GTLIEE1228 pKa = 5.51DD1229 pKa = 4.28GSITSTMAGSTISIAGSTNTSDD1251 pKa = 3.14AGVAIGAVPIPSDD1264 pKa = 3.15QSVFFNTPYY1273 pKa = 9.35EE1274 pKa = 4.28TGPVTISGAPGGTLTLRR1291 pKa = 11.84AANDD1295 pKa = 3.4NTSQSLLARR1304 pKa = 11.84GATITGGGGTLAISSASVDD1323 pKa = 3.4PATFALSSQDD1333 pKa = 3.58APPITLFGTSGGMSIDD1349 pKa = 3.41AQTFAAFSQFGSLTLGSSTQTGLITVNGQCASASSSCQPVKK1390 pKa = 9.67PTLDD1394 pKa = 3.47MNLTLANAGAGSQGIQLPYY1413 pKa = 10.63GLDD1416 pKa = 3.49VGNHH1420 pKa = 4.74TLTLQSAGNVTDD1432 pKa = 4.24PGGIDD1437 pKa = 3.29AAQLVLAGPGTFTLTDD1453 pKa = 3.56PQNNVGEE1460 pKa = 4.39IAFEE1464 pKa = 3.88NAGNVDD1470 pKa = 4.01FSNPVGFVIGGGNSTLTGNLVAQATTGNISLGSTAAGGPTTNLSAGGTIDD1520 pKa = 4.94LVMEE1524 pKa = 4.43NGVFVSPDD1532 pKa = 3.18GGTVSATNGWRR1543 pKa = 11.84IWASTWSGEE1552 pKa = 4.04TRR1554 pKa = 11.84GNVQPNTSQPNFYY1567 pKa = 10.66GCTFGAGCSWGGTVPSSGDD1586 pKa = 3.08HH1587 pKa = 4.97YY1588 pKa = 11.35VYY1590 pKa = 10.63VARR1593 pKa = 11.84PTVTVTADD1601 pKa = 2.95GEE1603 pKa = 4.7TRR1605 pKa = 11.84VVGAPDD1611 pKa = 3.43PAFTYY1616 pKa = 10.45AVSGLINGDD1625 pKa = 3.41TAAGTLSGTLTSPATPGSPAGRR1647 pKa = 11.84YY1648 pKa = 9.86AIDD1651 pKa = 3.48PAFLSSVGYY1660 pKa = 9.87IVNDD1664 pKa = 3.3VPGTLTVTAAPTSTQSLAPRR1684 pKa = 11.84FAEE1687 pKa = 4.31DD1688 pKa = 4.4LPMQVGAQQSYY1699 pKa = 10.03FGNEE1703 pKa = 3.62EE1704 pKa = 3.69KK1705 pKa = 10.55TFVYY1709 pKa = 10.29EE1710 pKa = 4.73NNLQGTNICVGSSEE1724 pKa = 4.46PLFTTAPPGDD1734 pKa = 4.16NQDD1737 pKa = 3.77LLAVEE1742 pKa = 4.47WRR1744 pKa = 11.84RR1745 pKa = 11.84VRR1747 pKa = 11.84SQPNLNSCLLLNSQHH1762 pKa = 6.21GCGDD1766 pKa = 3.83FF1767 pKa = 3.65

Molecular weight: 170.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2W7GVK9|A0A2W7GVK9_9BURK 2Fe-2S ferredoxin OS=Paraburkholderia sp. PDC91 OX=2135498 GN=C7514_101623 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 8.74TAGGRR28 pKa = 11.84KK29 pKa = 9.04VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6876

0

6876

2242271

29

4351

326.1

35.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.978 ± 0.05

0.906 ± 0.009

5.599 ± 0.025

4.862 ± 0.034

3.633 ± 0.022

8.478 ± 0.041

2.321 ± 0.015

4.648 ± 0.023

2.672 ± 0.026

10.173 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.287 ± 0.013

2.699 ± 0.022

5.247 ± 0.023

3.477 ± 0.021

7.208 ± 0.037

5.608 ± 0.026

5.471 ± 0.035

7.934 ± 0.024

1.375 ± 0.013

2.424 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski