Carnation etched ring virus (CERV)
Average proteome isoelectric point is 7.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P05399|CAPSD_CERV Probable capsid protein OS=Carnation etched ring virus OX=10640 GN=ORF IV PE=3 SV=1
MM1 pKa = 7.37 NLATIASEE9 pKa = 4.07 IEE11 pKa = 4.3 VVKK14 pKa = 10.08 TNQKK18 pKa = 9.26 TIEE21 pKa = 4.19 SKK23 pKa = 10.25 IDD25 pKa = 3.33 QILAKK30 pKa = 10.16 IGSTPDD36 pKa = 2.97 EE37 pKa = 4.49 SSNLEE42 pKa = 3.89 SVAAKK47 pKa = 10.14 IISDD51 pKa = 3.52 LTKK54 pKa = 10.64 EE55 pKa = 4.13 MKK57 pKa = 9.81 EE58 pKa = 3.94 CHH60 pKa = 6.23 CNKK63 pKa = 10.26 EE64 pKa = 3.84 IVEE67 pKa = 4.29 ILNKK71 pKa = 10.27 DD72 pKa = 3.3 KK73 pKa = 11.27 AIIPSPEE80 pKa = 3.44 QDD82 pKa = 3.62 SIQKK86 pKa = 10.22 RR87 pKa = 11.84 LSEE90 pKa = 3.98 PQYY93 pKa = 9.58 TFPNFDD99 pKa = 3.32 VGNEE103 pKa = 3.97 GMGSSTNPNALKK115 pKa = 10.03 WPPTEE120 pKa = 5.67 KK121 pKa = 10.11 PQPWPPRR128 pKa = 3.54
Molecular weight: 14.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.113
IPC2_protein 5.143
IPC_protein 4.978
Toseland 4.863
ProMoST 5.067
Dawson 4.914
Bjellqvist 5.067
Wikipedia 4.749
Rodwell 4.838
Grimsley 4.774
Solomon 4.914
Lehninger 4.863
Nozaki 5.029
DTASelect 5.118
Thurlkill 4.851
EMBOSS 4.774
Sillero 5.105
Patrickios 4.469
IPC_peptide 4.927
IPC2_peptide 5.105
IPC2.peptide.svr19 5.116
Protein with the highest isoelectric point:
>sp|P05398|VAP_CERV Virion-associated protein OS=Carnation etched ring virus OX=10640 GN=ORF III PE=3 SV=1
MM1 pKa = 7.54 SLTTYY6 pKa = 8.85 PHH8 pKa = 7.01 IYY10 pKa = 10.05 KK11 pKa = 10.16 KK12 pKa = 10.15 EE13 pKa = 3.89 QILKK17 pKa = 10.42 LKK19 pKa = 10.43 RR20 pKa = 11.84 LNKK23 pKa = 10.09 LSNDD27 pKa = 2.92 RR28 pKa = 11.84 KK29 pKa = 10.44 FFFSSVKK36 pKa = 9.17 GTLPGIISHH45 pKa = 6.74 CNNINEE51 pKa = 4.29 ILGRR55 pKa = 11.84 CYY57 pKa = 10.7 LGICKK62 pKa = 10.2 LNSFFGLSKK71 pKa = 10.97 DD72 pKa = 3.69 PSDD75 pKa = 3.72 KK76 pKa = 11.1 LSVSKK81 pKa = 11.0 SPSVYY86 pKa = 9.78 TLPSKK91 pKa = 10.4 IFKK94 pKa = 10.33 EE95 pKa = 4.46 GGGNGDD101 pKa = 3.38 NTTTQTDD108 pKa = 3.48 ILKK111 pKa = 10.04 NAQDD115 pKa = 3.64 QVILSKK121 pKa = 10.88 KK122 pKa = 9.36 IDD124 pKa = 3.65 EE125 pKa = 4.88 LQTQVKK131 pKa = 8.76 EE132 pKa = 4.11 LSSKK136 pKa = 10.41 IEE138 pKa = 3.98 PEE140 pKa = 4.03 PLTKK144 pKa = 10.39 EE145 pKa = 4.45 DD146 pKa = 3.05 IKK148 pKa = 10.74 KK149 pKa = 9.04 TYY151 pKa = 7.29 EE152 pKa = 3.76 TLSRR156 pKa = 11.84 IEE158 pKa = 4.48 SGLKK162 pKa = 10.31 GIIGIEE168 pKa = 3.85
Molecular weight: 18.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.719
IPC2_protein 8.521
IPC_protein 8.419
Toseland 9.311
ProMoST 8.975
Dawson 9.531
Bjellqvist 9.253
Wikipedia 9.648
Rodwell 9.999
Grimsley 9.56
Solomon 9.604
Lehninger 9.575
Nozaki 9.487
DTASelect 9.18
Thurlkill 9.414
EMBOSS 9.736
Sillero 9.516
Patrickios 5.474
IPC_peptide 9.604
IPC2_peptide 7.775
IPC2.peptide.svr19 7.837
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2264
128
659
377.3
43.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.196 ± 0.362
1.723 ± 0.364
4.947 ± 0.472
8.348 ± 0.883
4.417 ± 0.451
4.373 ± 0.345
1.943 ± 0.467
7.818 ± 0.538
11.042 ± 0.325
8.701 ± 0.574
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.502 ± 0.137
5.919 ± 0.444
4.859 ± 0.459
3.931 ± 0.252
3.534 ± 0.306
9.231 ± 0.687
4.549 ± 0.469
4.196 ± 0.269
0.883 ± 0.189
3.887 ± 0.375
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here