Pelagivirga sediminicola
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3729 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T7G4T3|A0A2T7G4T3_9RHOB Phosphoenolpyruvate carboxykinase (ATP) OS=Pelagivirga sediminicola OX=2170575 GN=pckA PE=3 SV=1
MM1 pKa = 7.86 KK2 pKa = 10.29 YY3 pKa = 10.47 LLSATAASALLAGTAFAGNIEE24 pKa = 4.57 SAPMEE29 pKa = 4.36 PVIAPAPAPVFTSPNWTGFYY49 pKa = 10.57 AGGQLGYY56 pKa = 11.02 ADD58 pKa = 3.99 ISGSGTGFAGDD69 pKa = 3.68 RR70 pKa = 11.84 DD71 pKa = 3.94 GDD73 pKa = 4.16 GVDD76 pKa = 4.87 DD77 pKa = 5.27 NVDD80 pKa = 3.21 ADD82 pKa = 3.97 GFPIPFGFSANGDD95 pKa = 3.62 GMIGGLTVGYY105 pKa = 10.02 DD106 pKa = 3.49 YY107 pKa = 11.93 DD108 pKa = 3.54 MGDD111 pKa = 3.37 WVVGAAADD119 pKa = 4.03 YY120 pKa = 10.27 DD121 pKa = 3.73 WTDD124 pKa = 2.86 ISVGGLALDD133 pKa = 5.22 DD134 pKa = 4.7 DD135 pKa = 6.03 DD136 pKa = 5.07 IAIGYY141 pKa = 7.35 GTTIEE146 pKa = 4.5 SIWRR150 pKa = 11.84 AKK152 pKa = 9.97 LRR154 pKa = 11.84 AGYY157 pKa = 10.45 KK158 pKa = 10.05 LGNGLLYY165 pKa = 9.94 ATAGYY170 pKa = 9.68 AQADD174 pKa = 3.92 TKK176 pKa = 11.47 DD177 pKa = 2.94 WGNTDD182 pKa = 3.47 GYY184 pKa = 10.96 FVGAGYY190 pKa = 10.61 EE191 pKa = 4.11 HH192 pKa = 7.19 MVTQNISLGGEE203 pKa = 3.83 ILYY206 pKa = 10.59 HH207 pKa = 6.15 EE208 pKa = 5.26 FDD210 pKa = 3.69 NFSGVNVPDD219 pKa = 3.68 YY220 pKa = 11.0 DD221 pKa = 4.07 IEE223 pKa = 4.24 ATTAQVRR230 pKa = 11.84 ATFRR234 pKa = 11.84 FF235 pKa = 3.67
Molecular weight: 24.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.752
IPC2_protein 3.668
IPC_protein 3.694
Toseland 3.452
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.681
Rodwell 3.516
Grimsley 3.363
Solomon 3.694
Lehninger 3.656
Nozaki 3.821
DTASelect 4.126
Thurlkill 3.528
EMBOSS 3.681
Sillero 3.821
Patrickios 0.477
IPC_peptide 3.694
IPC2_peptide 3.795
IPC2.peptide.svr19 3.736
Protein with the highest isoelectric point:
>tr|A0A2T7G739|A0A2T7G739_9RHOB Uncharacterized protein OS=Pelagivirga sediminicola OX=2170575 GN=DC366_08280 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3729
0
3729
1158833
26
1891
310.8
33.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.84 ± 0.064
0.904 ± 0.013
6.174 ± 0.034
5.653 ± 0.037
3.557 ± 0.026
8.823 ± 0.036
2.074 ± 0.019
5.316 ± 0.026
3.023 ± 0.034
10.023 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.91 ± 0.021
2.403 ± 0.02
5.112 ± 0.026
3.219 ± 0.021
7.024 ± 0.043
5.028 ± 0.028
5.327 ± 0.023
6.966 ± 0.035
1.411 ± 0.015
2.211 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here