Limnohabitans sp. 2KL-17

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Limnohabitans; unclassified Limnohabitans

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3184 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A315DSE8|A0A315DSE8_9BURK Resolvase OS=Limnohabitans sp. 2KL-17 OX=1100704 GN=B9Z45_16290 PE=4 SV=1
MM1 pKa = 8.09VEE3 pKa = 3.96VSAIAITNLSDD14 pKa = 3.82SSVAEE19 pKa = 3.85NAAYY23 pKa = 8.96TSSTPSLSGTPIGAVTYY40 pKa = 10.46SLGGADD46 pKa = 3.71SALFTLNTGTGVVSMVARR64 pKa = 11.84NFEE67 pKa = 4.21SPDD70 pKa = 3.3DD71 pKa = 4.17AGANNVYY78 pKa = 10.61NYY80 pKa = 9.95TLTATDD86 pKa = 3.97ADD88 pKa = 4.47GNSADD93 pKa = 3.68QAVVLSVVDD102 pKa = 4.45FNDD105 pKa = 3.24NAPVFSSGATGSVPEE120 pKa = 4.24NAATTRR126 pKa = 11.84EE127 pKa = 4.34IYY129 pKa = 8.88TASTTDD135 pKa = 3.47ADD137 pKa = 3.63GTAANRR143 pKa = 11.84NVVYY147 pKa = 10.23SLKK150 pKa = 10.8AATGDD155 pKa = 3.51IALLDD160 pKa = 3.76IDD162 pKa = 4.22SATGKK167 pKa = 8.53VTLKK171 pKa = 10.91NSANYY176 pKa = 7.53EE177 pKa = 4.18AKK179 pKa = 10.12SSYY182 pKa = 10.87SFTVVATNVATSGTLSTEE200 pKa = 3.73QAVIANVTNVNDD212 pKa = 3.91APTVSAALASNASEE226 pKa = 4.33GDD228 pKa = 3.01SSYY231 pKa = 11.17TVNLLQGATDD241 pKa = 3.71ADD243 pKa = 3.78AGEE246 pKa = 4.3TATLSTQSLTYY257 pKa = 9.86QVNGGTASATLPAGVSITGNTLTVDD282 pKa = 3.61PANAVFNSLAFGQSQVIVASYY303 pKa = 10.66QVTDD307 pKa = 3.49VQGASVNQTATLTISGTNDD326 pKa = 3.1APTLSANTQTASYY339 pKa = 10.61ANDD342 pKa = 3.06ATAQTVFSGAFISAVDD358 pKa = 3.62AGQTIKK364 pKa = 10.88GLKK367 pKa = 8.54FTVSGITDD375 pKa = 3.75ASSEE379 pKa = 4.58LITVDD384 pKa = 3.01GTEE387 pKa = 4.17FSLTHH392 pKa = 5.74ATSGTTAAGSIVYY405 pKa = 9.98SVTVTGSTATITLTHH420 pKa = 6.15TAGLTAAQAQALVNGIAYY438 pKa = 9.88RR439 pKa = 11.84NSSTNGMSANRR450 pKa = 11.84VVTLTEE456 pKa = 3.97ITDD459 pKa = 3.57SGIDD463 pKa = 3.64NNVTTLNLATTLQDD477 pKa = 3.32ALAPNVVSVANNVSSLVKK495 pKa = 10.37IGTGDD500 pKa = 3.16VTYY503 pKa = 9.21TYY505 pKa = 10.05TFSEE509 pKa = 4.99VVTGLTSADD518 pKa = 3.29FTVTHH523 pKa = 6.02GTVASISGSGTTWTVVVTPAANVASGKK550 pKa = 10.38LVVVLNAGSVQDD562 pKa = 3.93SVGNTSLQHH571 pKa = 5.81TDD573 pKa = 2.86STVSIDD579 pKa = 3.27TVAPVVSINTIANNDD594 pKa = 3.08IVNAAKK600 pKa = 10.24KK601 pKa = 9.63AAGVTVSGTSDD612 pKa = 3.5AEE614 pKa = 4.2VGQDD618 pKa = 4.21VSVAWGGISKK628 pKa = 8.83TGKK631 pKa = 10.0VLGDD635 pKa = 3.66GTWQVTFAAVEE646 pKa = 4.28VPGDD650 pKa = 3.8VASSTMTANVSDD662 pKa = 3.95QAGNAAVAATRR673 pKa = 11.84SVRR676 pKa = 11.84IDD678 pKa = 3.16TTAPTISITPPLTNGATVDD697 pKa = 3.63NDD699 pKa = 3.44ADD701 pKa = 4.67AILNKK706 pKa = 10.52AEE708 pKa = 4.52LDD710 pKa = 3.42AVLPNGYY717 pKa = 9.24LAVIGVTNAEE727 pKa = 4.07AGQTVTAFFNSKK739 pKa = 10.07SYY741 pKa = 9.21TATVIAGTPNNTWAVQVPKK760 pKa = 10.85ADD762 pKa = 4.22LLALAHH768 pKa = 6.33GNSYY772 pKa = 10.65SISATVSDD780 pKa = 3.87AAGNPVATPASSTLEE795 pKa = 4.14VKK797 pKa = 10.37LAPPDD802 pKa = 3.58VPTVVQLNTNDD813 pKa = 3.74LTPVITGLAQKK824 pKa = 10.1EE825 pKa = 4.19DD826 pKa = 3.85PADD829 pKa = 3.65VNSYY833 pKa = 10.26IALEE837 pKa = 4.45AGDD840 pKa = 5.19AITVTVGGQTYY851 pKa = 8.36TLTLGANAGASTPAGLTYY869 pKa = 10.65SGSTWSLAVPTALTQGQYY887 pKa = 10.91NVGVSVNAVGYY898 pKa = 7.42STPKK902 pKa = 9.65TDD904 pKa = 2.9ISSSEE909 pKa = 3.98LVIKK913 pKa = 9.92TDD915 pKa = 3.79APAITINTIAGDD927 pKa = 3.7NVINIAEE934 pKa = 4.37HH935 pKa = 6.28GGAITLSGTATDD947 pKa = 4.91NIPGVTPAVNTAAGKK962 pKa = 9.81NLSLTLAGVTYY973 pKa = 10.96SDD975 pKa = 4.5IAIQSDD981 pKa = 4.34GTWHH985 pKa = 6.31VDD987 pKa = 3.29VPAASVALLSAASYY1001 pKa = 10.26SAVVNYY1007 pKa = 9.49TGVYY1011 pKa = 10.14GNTGSQSQSLALDD1024 pKa = 3.82LVKK1027 pKa = 10.12PATPDD1032 pKa = 3.11AVLTTDD1038 pKa = 3.87TGVTDD1043 pKa = 4.13AAHH1046 pKa = 5.58VTQSAAITAPTNTEE1060 pKa = 3.32ADD1062 pKa = 2.95ATLQYY1067 pKa = 10.65RR1068 pKa = 11.84ITKK1071 pKa = 10.21SGEE1074 pKa = 3.95TEE1076 pKa = 4.3GAWGSYY1082 pKa = 9.2AAPTVDD1088 pKa = 2.66GAYY1091 pKa = 8.96TVEE1094 pKa = 4.15VRR1096 pKa = 11.84QTDD1099 pKa = 3.49AAGNPSDD1106 pKa = 3.88IQTLSIVLDD1115 pKa = 3.32NTAPVFGSLATANVNEE1131 pKa = 4.22NVAAGTVVYY1140 pKa = 8.39TANASDD1146 pKa = 3.65ANAGVVYY1153 pKa = 10.13SLKK1156 pKa = 10.87AGVGDD1161 pKa = 4.84AIKK1164 pKa = 9.38FTIDD1168 pKa = 2.89AATGQVKK1175 pKa = 9.74LQEE1178 pKa = 4.19VPNYY1182 pKa = 9.27EE1183 pKa = 4.08GQISYY1188 pKa = 11.08AFTVVATDD1196 pKa = 3.37KK1197 pKa = 11.25AGNATEE1203 pKa = 3.93QAVAVTVTDD1212 pKa = 3.72VVEE1215 pKa = 4.43VSAIAITNLSDD1226 pKa = 3.82SSVAEE1231 pKa = 3.85NAAYY1235 pKa = 8.96TSSTPSLSGTT1245 pKa = 3.57

Molecular weight: 124.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A315EL79|A0A315EL79_9BURK Aldehyde-activating protein OS=Limnohabitans sp. 2KL-17 OX=1100704 GN=B9Z45_07890 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 7.79TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3184

0

3184

1042381

31

4604

327.4

35.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.705 ± 0.064

0.972 ± 0.016

5.108 ± 0.033

5.117 ± 0.041

3.56 ± 0.029

8.084 ± 0.052

2.364 ± 0.025

4.582 ± 0.039

4.053 ± 0.048

10.7 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.796 ± 0.026

2.999 ± 0.035

4.95 ± 0.036

4.653 ± 0.032

5.769 ± 0.048

5.767 ± 0.045

5.48 ± 0.051

7.613 ± 0.032

1.563 ± 0.021

2.165 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski