Roseivivax halodurans JCM 10272
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4304 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|X7EL04|X7EL04_9RHOB LacI family transcription regulator OS=Roseivivax halodurans JCM 10272 OX=1449350 GN=OCH239_01700 PE=4 SV=1
MM1 pKa = 6.88 FTRR4 pKa = 11.84 AISTSVAALLLAGAAHH20 pKa = 6.56 AQDD23 pKa = 3.21 QSIIVQSTTSTANSGLYY40 pKa = 10.14 DD41 pKa = 3.46 HH42 pKa = 7.7 LIPIFEE48 pKa = 4.47 EE49 pKa = 4.18 EE50 pKa = 3.89 SGITVNVVAVGTGQAITNAEE70 pKa = 4.05 NCDD73 pKa = 3.23 GDD75 pKa = 4.51 LLLVHH80 pKa = 7.15 AKK82 pKa = 9.34 PAEE85 pKa = 4.02 EE86 pKa = 4.26 AFVEE90 pKa = 4.2 AGYY93 pKa = 8.52 GTEE96 pKa = 4.46 RR97 pKa = 11.84 TDD99 pKa = 4.25 LMYY102 pKa = 11.02 NDD104 pKa = 4.27 FVIVGPAEE112 pKa = 4.38 DD113 pKa = 3.78 PAGVDD118 pKa = 3.91 GMEE121 pKa = 4.71 DD122 pKa = 3.36 AQDD125 pKa = 3.84 ALSQIAGSGATFASRR140 pKa = 11.84 GDD142 pKa = 3.9 DD143 pKa = 3.29 SGTHH147 pKa = 4.88 QKK149 pKa = 10.24 EE150 pKa = 3.67 LSLWEE155 pKa = 4.35 GTDD158 pKa = 3.57 ADD160 pKa = 4.07 PTSGSGEE167 pKa = 4.14 WYY169 pKa = 10.28 RR170 pKa = 11.84 EE171 pKa = 4.0 TGSGMGATLNAGIGMGAYY189 pKa = 10.58 VMTDD193 pKa = 2.74 RR194 pKa = 11.84 ATWISFEE201 pKa = 4.09 NKK203 pKa = 8.25 QDD205 pKa = 3.68 YY206 pKa = 10.03 EE207 pKa = 4.22 ILVEE211 pKa = 4.23 GDD213 pKa = 2.96 EE214 pKa = 5.6 DD215 pKa = 4.77 LFNQYY220 pKa = 9.89 GVIPVSPDD228 pKa = 2.63 HH229 pKa = 6.69 CPSVNIEE236 pKa = 3.98 AAEE239 pKa = 4.17 TFADD243 pKa = 3.41 WLLSAEE249 pKa = 4.08 GQEE252 pKa = 4.71 AIGAYY257 pKa = 8.45 EE258 pKa = 4.15 VEE260 pKa = 4.39 GQQLFFPNAPASS272 pKa = 3.55
Molecular weight: 28.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.711
IPC2_protein 3.77
IPC_protein 3.757
Toseland 3.554
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.63
Rodwell 3.592
Grimsley 3.465
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.024
Thurlkill 3.592
EMBOSS 3.643
Sillero 3.872
Patrickios 1.125
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.79
Protein with the highest isoelectric point:
>tr|X7EAB8|X7EAB8_9RHOB Integrase OS=Roseivivax halodurans JCM 10272 OX=1449350 GN=OCH239_14730 PE=4 SV=1
MM1 pKa = 7.57 SRR3 pKa = 11.84 TNLIAGVRR11 pKa = 11.84 RR12 pKa = 11.84 GTGRR16 pKa = 11.84 ATGVRR21 pKa = 11.84 RR22 pKa = 11.84 AGWRR26 pKa = 11.84 AGPRR30 pKa = 11.84 RR31 pKa = 11.84 MPSIGRR37 pKa = 11.84 VV38 pKa = 3.14
Molecular weight: 4.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.513
IPC2_protein 11.184
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.457
Grimsley 12.998
Solomon 13.451
Lehninger 13.349
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.193
IPC_peptide 13.451
IPC2_peptide 12.442
IPC2.peptide.svr19 9.197
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4304
0
4304
1296111
28
3670
301.1
32.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.66 ± 0.054
0.865 ± 0.013
6.121 ± 0.038
6.498 ± 0.04
3.567 ± 0.025
8.96 ± 0.039
2.008 ± 0.019
4.906 ± 0.027
2.599 ± 0.031
10.015 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.651 ± 0.019
2.234 ± 0.02
5.167 ± 0.029
2.863 ± 0.018
7.399 ± 0.044
5.248 ± 0.026
5.433 ± 0.023
7.293 ± 0.028
1.412 ± 0.014
2.102 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here