Lysobacter maris
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3120 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U9TC59|A0A2U9TC59_9GAMM DUF218 domain-containing protein OS=Lysobacter maris OX=1605891 GN=C9I47_3123 PE=4 SV=1
MM1 pKa = 7.57 SSQHH5 pKa = 5.36 TAFHH9 pKa = 6.63 APGGRR14 pKa = 11.84 IQGIRR19 pKa = 11.84 EE20 pKa = 3.86 TAPAPLAALTAIATMATMLAMGQARR45 pKa = 11.84 AGEE48 pKa = 4.18 LSGSVSLTSDD58 pKa = 3.38 YY59 pKa = 11.02 LFRR62 pKa = 11.84 GITQTDD68 pKa = 3.82 EE69 pKa = 4.27 EE70 pKa = 4.71 PALQAGVEE78 pKa = 4.19 YY79 pKa = 8.9 ATDD82 pKa = 3.23 SGFYY86 pKa = 10.08 AGVWGSSISWLSDD99 pKa = 2.95 SDD101 pKa = 4.18 PDD103 pKa = 4.07 ISSQLEE109 pKa = 3.74 LDD111 pKa = 4.08 GYY113 pKa = 10.98 LGLRR117 pKa = 11.84 GEE119 pKa = 4.51 FADD122 pKa = 5.7 SGVSWDD128 pKa = 3.79 VGAIHH133 pKa = 6.21 YY134 pKa = 7.53 WYY136 pKa = 9.81 PGDD139 pKa = 3.8 YY140 pKa = 10.42 PSGFNSADD148 pKa = 3.33 TTEE151 pKa = 4.55 VYY153 pKa = 10.66 AGLGWSVLSAKK164 pKa = 10.24 YY165 pKa = 10.44 SYY167 pKa = 11.62 ALTDD171 pKa = 3.83 LFGIPDD177 pKa = 3.72 SDD179 pKa = 3.78 GSHH182 pKa = 6.78 ALDD185 pKa = 5.49 LGASWEE191 pKa = 4.43 FVPSWTLDD199 pKa = 3.48 AAVGKK204 pKa = 9.84 QWVEE208 pKa = 3.79 NLDD211 pKa = 4.0 GGDD214 pKa = 3.42 YY215 pKa = 10.55 AYY217 pKa = 9.69 WKK219 pKa = 10.28 LGVGKK224 pKa = 10.37 AFDD227 pKa = 3.74 NGIDD231 pKa = 4.28 VAVAWNDD238 pKa = 3.02 NDD240 pKa = 5.95 LIGPDD245 pKa = 3.38 EE246 pKa = 4.38 TLTVAITKK254 pKa = 10.07 SFF256 pKa = 3.29
Molecular weight: 27.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.706
IPC2_protein 3.859
IPC_protein 3.872
Toseland 3.656
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.821
Rodwell 3.694
Grimsley 3.567
Solomon 3.872
Lehninger 3.821
Nozaki 3.986
DTASelect 4.253
Thurlkill 3.706
EMBOSS 3.821
Sillero 3.999
Patrickios 1.163
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.882
Protein with the highest isoelectric point:
>tr|A0A2U9TK15|A0A2U9TK15_9GAMM Short-chain fatty acid transporter OS=Lysobacter maris OX=1605891 GN=C9I47_2654 PE=4 SV=1
MM1 pKa = 7.05 ATKK4 pKa = 10.25 KK5 pKa = 10.11 AAKK8 pKa = 9.45 KK9 pKa = 10.34 KK10 pKa = 9.45 PAAKK14 pKa = 9.97 KK15 pKa = 9.35 AAKK18 pKa = 9.77 KK19 pKa = 9.31 VAKK22 pKa = 10.13 KK23 pKa = 9.77 SAARR27 pKa = 11.84 KK28 pKa = 6.29 TVKK31 pKa = 10.32 KK32 pKa = 10.01 AAKK35 pKa = 9.17 KK36 pKa = 9.25 AAKK39 pKa = 8.65 KK40 pKa = 7.86 TVRR43 pKa = 11.84 KK44 pKa = 9.12 AAAKK48 pKa = 8.44 KK49 pKa = 7.75 TVRR52 pKa = 11.84 KK53 pKa = 9.01 AAKK56 pKa = 8.7 KK57 pKa = 7.36 TVKK60 pKa = 10.18 KK61 pKa = 10.42 AAAKK65 pKa = 8.5 KK66 pKa = 7.09 TAKK69 pKa = 10.17 KK70 pKa = 9.86 AAKK73 pKa = 8.57 KK74 pKa = 7.22 TAKK77 pKa = 9.98 KK78 pKa = 9.81 AAKK81 pKa = 8.57 KK82 pKa = 7.77 TVRR85 pKa = 11.84 KK86 pKa = 9.23 AAAKK90 pKa = 8.48 KK91 pKa = 7.19 TAKK94 pKa = 10.06 KK95 pKa = 9.87 AAKK98 pKa = 8.71 KK99 pKa = 7.79 TVKK102 pKa = 10.16 KK103 pKa = 10.12 AAKK106 pKa = 8.27 KK107 pKa = 7.53 TVRR110 pKa = 11.84 KK111 pKa = 9.23 AAAKK115 pKa = 8.48 KK116 pKa = 7.19 TAKK119 pKa = 10.06 KK120 pKa = 9.87 AAKK123 pKa = 8.71 KK124 pKa = 7.79 TVKK127 pKa = 10.29 KK128 pKa = 10.09 AAKK131 pKa = 8.25 KK132 pKa = 6.92 TARR135 pKa = 11.84 KK136 pKa = 9.07 AATKK140 pKa = 8.86 KK141 pKa = 7.88 TAKK144 pKa = 9.96 KK145 pKa = 9.75 AAKK148 pKa = 8.71 KK149 pKa = 7.79 TVKK152 pKa = 10.16 KK153 pKa = 10.18 AAKK156 pKa = 8.65 KK157 pKa = 7.59 TVKK160 pKa = 10.23 KK161 pKa = 10.11 AAKK164 pKa = 9.75 KK165 pKa = 8.7 ATKK168 pKa = 10.17 KK169 pKa = 9.92 SAKK172 pKa = 9.83 KK173 pKa = 9.46 PARR176 pKa = 11.84 KK177 pKa = 6.86 TAKK180 pKa = 10.04 RR181 pKa = 11.84 AAKK184 pKa = 9.59 KK185 pKa = 9.68 AAPAAMPATPAPMII199 pKa = 4.39
Molecular weight: 20.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.411
IPC2_protein 11.433
IPC_protein 12.691
Toseland 12.983
ProMoST 13.364
Dawson 12.998
Bjellqvist 12.896
Wikipedia 13.378
Rodwell 13.451
Grimsley 13.027
Solomon 13.393
Lehninger 13.305
Nozaki 12.969
DTASelect 12.896
Thurlkill 12.969
EMBOSS 13.451
Sillero 12.969
Patrickios 13.144
IPC_peptide 13.408
IPC2_peptide 12.34
IPC2.peptide.svr19 8.996
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3120
0
3120
1080331
31
5326
346.3
37.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.313 ± 0.063
0.821 ± 0.012
6.293 ± 0.038
5.602 ± 0.047
3.287 ± 0.028
8.945 ± 0.041
2.241 ± 0.021
4.035 ± 0.028
2.268 ± 0.038
10.603 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.143 ± 0.022
2.343 ± 0.033
5.44 ± 0.033
3.355 ± 0.026
8.129 ± 0.061
5.141 ± 0.04
4.757 ± 0.084
7.395 ± 0.035
1.537 ± 0.022
2.354 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here