Gordonia phage Margaret

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4Q6S7|A0A2Z4Q6S7_9CAUD Tail assembly chaperone OS=Gordonia phage Margaret OX=2201428 GN=18 PE=4 SV=1
MM1 pKa = 7.15SQQTLAEE8 pKa = 4.07LQRR11 pKa = 11.84AIEE14 pKa = 4.03AHH16 pKa = 6.21VGSQFAEE23 pKa = 4.41DD24 pKa = 4.79EE25 pKa = 4.44PDD27 pKa = 3.18ATPPIVNCWFLGVGLSGVSDD47 pKa = 3.33TSIVHH52 pKa = 6.6DD53 pKa = 3.67VTYY56 pKa = 10.87VVSDD60 pKa = 3.77SPAYY64 pKa = 10.56ASMGACDD71 pKa = 4.62LALEE75 pKa = 4.32QARR78 pKa = 11.84SHH80 pKa = 5.71VLYY83 pKa = 11.1GNDD86 pKa = 4.08DD87 pKa = 3.69GAA89 pKa = 5.71

Molecular weight: 9.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4Q5C1|A0A2Z4Q5C1_9CAUD MazG-like nucleotide pyrophosphohydrolase OS=Gordonia phage Margaret OX=2201428 GN=56 PE=4 SV=1
MM1 pKa = 7.25ARR3 pKa = 11.84KK4 pKa = 9.44RR5 pKa = 11.84SSKK8 pKa = 10.05KK9 pKa = 9.49RR10 pKa = 11.84SGSKK14 pKa = 10.33GFRR17 pKa = 11.84SKK19 pKa = 11.13AQWRR23 pKa = 11.84WAFATKK29 pKa = 10.3KK30 pKa = 9.63SWARR34 pKa = 11.84KK35 pKa = 8.24HH36 pKa = 5.86AHH38 pKa = 4.65QTRR41 pKa = 11.84GGYY44 pKa = 9.31RR45 pKa = 11.84RR46 pKa = 11.84LPRR49 pKa = 11.84KK50 pKa = 9.86RR51 pKa = 11.84GRR53 pKa = 3.38

Molecular weight: 6.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

15184

33

1401

202.5

22.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.399 ± 0.395

0.889 ± 0.139

6.948 ± 0.323

5.447 ± 0.409

3.062 ± 0.17

8.173 ± 0.606

2.127 ± 0.201

4.992 ± 0.285

4.413 ± 0.264

7.732 ± 0.247

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.74 ± 0.136

2.95 ± 0.173

5.427 ± 0.183

3.826 ± 0.211

7.35 ± 0.394

5.611 ± 0.21

5.987 ± 0.31

6.882 ± 0.218

2.259 ± 0.168

2.786 ± 0.194
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski