Amino acid dipepetide frequency for Gordonia phage Margaret

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.077AlaAla: 10.077 ± 0.99
0.856AlaCys: 0.856 ± 0.274
7.377AlaAsp: 7.377 ± 0.786
5.137AlaGlu: 5.137 ± 0.617
3.82AlaPhe: 3.82 ± 0.647
8.43AlaGly: 8.43 ± 0.86
2.569AlaHis: 2.569 ± 0.476
6.85AlaIle: 6.85 ± 0.944
4.479AlaLys: 4.479 ± 0.657
8.43AlaLeu: 8.43 ± 0.752
2.832AlaMet: 2.832 ± 0.362
2.964AlaAsn: 2.964 ± 0.336
4.545AlaPro: 4.545 ± 0.597
5.071AlaGln: 5.071 ± 0.672
7.838AlaArg: 7.838 ± 0.803
5.006AlaSer: 5.006 ± 0.719
5.796AlaThr: 5.796 ± 0.666
7.113AlaVal: 7.113 ± 0.649
2.239AlaTrp: 2.239 ± 0.388
2.569AlaTyr: 2.569 ± 0.434
0.0AlaXaa: 0.0 ± 0.0
Cys
1.12CysAla: 1.12 ± 0.368
0.0CysCys: 0.0 ± 0.0
0.856CysAsp: 0.856 ± 0.238
0.198CysGlu: 0.198 ± 0.132
0.461CysPhe: 0.461 ± 0.212
0.922CysGly: 0.922 ± 0.296
0.066CysHis: 0.066 ± 0.069
0.461CysIle: 0.461 ± 0.184
0.263CysLys: 0.263 ± 0.136
0.527CysLeu: 0.527 ± 0.164
0.263CysMet: 0.263 ± 0.132
0.461CysAsn: 0.461 ± 0.182
0.395CysPro: 0.395 ± 0.18
0.395CysGln: 0.395 ± 0.18
0.724CysArg: 0.724 ± 0.255
0.329CysSer: 0.329 ± 0.174
0.461CysThr: 0.461 ± 0.197
0.395CysVal: 0.395 ± 0.174
0.198CysTrp: 0.198 ± 0.093
0.395CysTyr: 0.395 ± 0.168
0.0CysXaa: 0.0 ± 0.0
Asp
8.233AspAla: 8.233 ± 0.671
0.527AspCys: 0.527 ± 0.201
5.071AspAsp: 5.071 ± 1.018
4.61AspGlu: 4.61 ± 0.759
1.844AspPhe: 1.844 ± 0.366
5.335AspGly: 5.335 ± 0.698
1.383AspHis: 1.383 ± 0.301
3.754AspIle: 3.754 ± 0.453
2.766AspLys: 2.766 ± 0.474
5.73AspLeu: 5.73 ± 0.718
2.042AspMet: 2.042 ± 0.408
1.976AspAsn: 1.976 ± 0.314
5.928AspPro: 5.928 ± 0.633
1.976AspGln: 1.976 ± 0.439
3.754AspArg: 3.754 ± 0.436
2.898AspSer: 2.898 ± 0.536
5.335AspThr: 5.335 ± 1.009
3.161AspVal: 3.161 ± 0.439
1.515AspTrp: 1.515 ± 0.364
1.647AspTyr: 1.647 ± 0.288
0.0AspXaa: 0.0 ± 0.0
Glu
5.796GluAla: 5.796 ± 0.77
0.724GluCys: 0.724 ± 0.183
4.545GluAsp: 4.545 ± 0.715
3.227GluGlu: 3.227 ± 0.586
1.712GluPhe: 1.712 ± 0.389
4.149GluGly: 4.149 ± 0.534
1.581GluHis: 1.581 ± 0.394
1.91GluIle: 1.91 ± 0.339
1.976GluLys: 1.976 ± 0.511
4.808GluLeu: 4.808 ± 0.518
1.712GluMet: 1.712 ± 0.346
1.251GluAsn: 1.251 ± 0.32
2.7GluPro: 2.7 ± 0.443
2.898GluGln: 2.898 ± 0.459
4.676GluArg: 4.676 ± 0.701
2.437GluSer: 2.437 ± 0.395
1.251GluThr: 1.251 ± 0.321
3.754GluVal: 3.754 ± 0.664
1.12GluTrp: 1.12 ± 0.321
2.239GluTyr: 2.239 ± 0.484
0.0GluXaa: 0.0 ± 0.0
Phe
2.964PheAla: 2.964 ± 0.567
0.593PheCys: 0.593 ± 0.229
2.832PheAsp: 2.832 ± 0.38
1.251PheGlu: 1.251 ± 0.315
0.856PhePhe: 0.856 ± 0.228
2.7PheGly: 2.7 ± 0.493
0.527PheHis: 0.527 ± 0.184
1.449PheIle: 1.449 ± 0.273
2.042PheLys: 2.042 ± 0.394
1.844PheLeu: 1.844 ± 0.33
0.79PheMet: 0.79 ± 0.182
1.054PheAsn: 1.054 ± 0.356
1.186PhePro: 1.186 ± 0.31
1.054PheGln: 1.054 ± 0.286
2.898PheArg: 2.898 ± 0.474
1.647PheSer: 1.647 ± 0.373
1.778PheThr: 1.778 ± 0.294
2.108PheVal: 2.108 ± 0.358
0.461PheTrp: 0.461 ± 0.151
0.593PheTyr: 0.593 ± 0.223
0.0PheXaa: 0.0 ± 0.0
Gly
8.101GlyAla: 8.101 ± 1.723
0.988GlyCys: 0.988 ± 0.283
6.586GlyAsp: 6.586 ± 0.94
3.557GlyGlu: 3.557 ± 0.479
3.161GlyPhe: 3.161 ± 0.418
5.73GlyGly: 5.73 ± 0.62
1.712GlyHis: 1.712 ± 0.358
4.281GlyIle: 4.281 ± 0.802
3.952GlyLys: 3.952 ± 0.547
6.455GlyLeu: 6.455 ± 0.777
1.712GlyMet: 1.712 ± 0.338
1.976GlyAsn: 1.976 ± 0.266
3.886GlyPro: 3.886 ± 1.257
2.503GlyGln: 2.503 ± 0.31
5.994GlyArg: 5.994 ± 0.66
4.61GlySer: 4.61 ± 0.583
6.257GlyThr: 6.257 ± 0.631
5.994GlyVal: 5.994 ± 0.526
1.581GlyTrp: 1.581 ± 0.415
2.635GlyTyr: 2.635 ± 0.401
0.0GlyXaa: 0.0 ± 0.0
His
1.844HisAla: 1.844 ± 0.365
0.198HisCys: 0.198 ± 0.116
1.449HisAsp: 1.449 ± 0.353
1.515HisGlu: 1.515 ± 0.35
0.659HisPhe: 0.659 ± 0.22
1.12HisGly: 1.12 ± 0.245
0.922HisHis: 0.922 ± 0.291
0.922HisIle: 0.922 ± 0.217
0.922HisLys: 0.922 ± 0.296
2.173HisLeu: 2.173 ± 0.39
0.988HisMet: 0.988 ± 0.247
0.461HisAsn: 0.461 ± 0.168
1.12HisPro: 1.12 ± 0.281
0.856HisGln: 0.856 ± 0.223
1.12HisArg: 1.12 ± 0.264
1.12HisSer: 1.12 ± 0.263
1.844HisThr: 1.844 ± 0.403
1.12HisVal: 1.12 ± 0.276
0.724HisTrp: 0.724 ± 0.191
0.198HisTyr: 0.198 ± 0.123
0.0HisXaa: 0.0 ± 0.0
Ile
6.455IleAla: 6.455 ± 0.868
0.395IleCys: 0.395 ± 0.154
4.018IleAsp: 4.018 ± 0.444
3.425IleGlu: 3.425 ± 0.465
0.724IlePhe: 0.724 ± 0.18
4.281IleGly: 4.281 ± 0.98
0.724IleHis: 0.724 ± 0.168
2.503IleIle: 2.503 ± 0.425
2.173IleLys: 2.173 ± 0.409
3.557IleLeu: 3.557 ± 0.512
0.724IleMet: 0.724 ± 0.235
1.449IleAsn: 1.449 ± 0.303
2.7IlePro: 2.7 ± 0.441
1.647IleGln: 1.647 ± 0.518
3.359IleArg: 3.359 ± 0.494
2.173IleSer: 2.173 ± 0.367
2.569IleThr: 2.569 ± 0.36
4.347IleVal: 4.347 ± 0.519
1.383IleTrp: 1.383 ± 0.256
1.317IleTyr: 1.317 ± 0.263
0.0IleXaa: 0.0 ± 0.0
Lys
3.952LysAla: 3.952 ± 0.493
0.198LysCys: 0.198 ± 0.118
2.503LysAsp: 2.503 ± 0.402
2.371LysGlu: 2.371 ± 0.406
1.515LysPhe: 1.515 ± 0.242
3.82LysGly: 3.82 ± 0.727
0.922LysHis: 0.922 ± 0.254
2.503LysIle: 2.503 ± 0.386
2.7LysLys: 2.7 ± 0.638
3.886LysLeu: 3.886 ± 0.587
0.922LysMet: 0.922 ± 0.247
1.186LysAsn: 1.186 ± 0.279
2.042LysPro: 2.042 ± 0.356
1.712LysGln: 1.712 ± 0.257
4.413LysArg: 4.413 ± 0.628
2.503LysSer: 2.503 ± 0.424
2.108LysThr: 2.108 ± 0.379
3.359LysVal: 3.359 ± 0.487
0.263LysTrp: 0.263 ± 0.156
1.251LysTyr: 1.251 ± 0.285
0.0LysXaa: 0.0 ± 0.0
Leu
8.233LeuAla: 8.233 ± 0.878
0.527LeuCys: 0.527 ± 0.254
5.137LeuAsp: 5.137 ± 0.662
4.808LeuGlu: 4.808 ± 0.75
1.647LeuPhe: 1.647 ± 0.406
6.059LeuGly: 6.059 ± 0.907
1.383LeuHis: 1.383 ± 0.297
4.084LeuIle: 4.084 ± 0.528
2.964LeuLys: 2.964 ± 0.485
5.467LeuLeu: 5.467 ± 0.731
2.305LeuMet: 2.305 ± 0.371
2.239LeuAsn: 2.239 ± 0.412
4.413LeuPro: 4.413 ± 0.541
2.371LeuGln: 2.371 ± 0.407
7.113LeuArg: 7.113 ± 0.706
4.742LeuSer: 4.742 ± 0.682
4.347LeuThr: 4.347 ± 0.64
5.598LeuVal: 5.598 ± 0.583
1.712LeuTrp: 1.712 ± 0.323
2.173LeuTyr: 2.173 ± 0.437
0.0LeuXaa: 0.0 ± 0.0
Met
2.832MetAla: 2.832 ± 0.343
0.198MetCys: 0.198 ± 0.106
1.251MetAsp: 1.251 ± 0.351
1.251MetGlu: 1.251 ± 0.257
0.856MetPhe: 0.856 ± 0.301
2.173MetGly: 2.173 ± 0.353
0.527MetHis: 0.527 ± 0.36
1.844MetIle: 1.844 ± 0.295
1.186MetLys: 1.186 ± 0.288
1.976MetLeu: 1.976 ± 0.308
0.593MetMet: 0.593 ± 0.174
0.988MetAsn: 0.988 ± 0.252
1.186MetPro: 1.186 ± 0.261
1.449MetGln: 1.449 ± 0.304
1.515MetArg: 1.515 ± 0.284
2.898MetSer: 2.898 ± 0.429
2.108MetThr: 2.108 ± 0.328
1.712MetVal: 1.712 ± 0.349
0.527MetTrp: 0.527 ± 0.203
0.329MetTyr: 0.329 ± 0.148
0.0MetXaa: 0.0 ± 0.0
Asn
3.622AsnAla: 3.622 ± 0.5
0.329AsnCys: 0.329 ± 0.128
2.042AsnAsp: 2.042 ± 0.441
1.712AsnGlu: 1.712 ± 0.376
1.317AsnPhe: 1.317 ± 0.308
2.108AsnGly: 2.108 ± 0.351
0.856AsnHis: 0.856 ± 0.256
1.054AsnIle: 1.054 ± 0.237
1.317AsnLys: 1.317 ± 0.294
1.778AsnLeu: 1.778 ± 0.291
0.922AsnMet: 0.922 ± 0.332
1.12AsnAsn: 1.12 ± 0.342
2.898AsnPro: 2.898 ± 0.494
0.724AsnGln: 0.724 ± 0.245
2.569AsnArg: 2.569 ± 0.426
1.251AsnSer: 1.251 ± 0.265
1.054AsnThr: 1.054 ± 0.26
1.778AsnVal: 1.778 ± 0.394
0.461AsnTrp: 0.461 ± 0.172
0.593AsnTyr: 0.593 ± 0.216
0.0AsnXaa: 0.0 ± 0.0
Pro
6.191ProAla: 6.191 ± 0.745
0.659ProCys: 0.659 ± 0.229
3.952ProAsp: 3.952 ± 0.512
3.161ProGlu: 3.161 ± 0.622
1.383ProPhe: 1.383 ± 0.254
5.203ProGly: 5.203 ± 0.687
0.922ProHis: 0.922 ± 0.255
2.437ProIle: 2.437 ± 0.354
2.898ProLys: 2.898 ± 0.549
3.293ProLeu: 3.293 ± 0.438
1.317ProMet: 1.317 ± 0.278
1.647ProAsn: 1.647 ± 0.356
3.293ProPro: 3.293 ± 0.601
2.7ProGln: 2.7 ± 0.588
3.161ProArg: 3.161 ± 0.478
3.161ProSer: 3.161 ± 0.388
2.305ProThr: 2.305 ± 0.371
3.359ProVal: 3.359 ± 0.552
1.12ProTrp: 1.12 ± 0.283
2.108ProTyr: 2.108 ± 0.335
0.0ProXaa: 0.0 ± 0.0
Gln
4.215GlnAla: 4.215 ± 0.628
0.198GlnCys: 0.198 ± 0.104
1.647GlnAsp: 1.647 ± 0.349
2.503GlnGlu: 2.503 ± 0.49
1.581GlnPhe: 1.581 ± 0.391
3.557GlnGly: 3.557 ± 1.485
0.593GlnHis: 0.593 ± 0.194
2.108GlnIle: 2.108 ± 0.377
0.724GlnLys: 0.724 ± 0.205
2.503GlnLeu: 2.503 ± 0.471
1.054GlnMet: 1.054 ± 0.29
0.922GlnAsn: 0.922 ± 0.35
1.383GlnPro: 1.383 ± 0.292
1.647GlnGln: 1.647 ± 0.374
3.227GlnArg: 3.227 ± 0.452
2.569GlnSer: 2.569 ± 0.459
2.437GlnThr: 2.437 ± 0.428
2.635GlnVal: 2.635 ± 0.387
1.317GlnTrp: 1.317 ± 0.301
1.449GlnTyr: 1.449 ± 0.354
0.0GlnXaa: 0.0 ± 0.0
Arg
7.443ArgAla: 7.443 ± 1.004
0.856ArgCys: 0.856 ± 0.274
4.347ArgAsp: 4.347 ± 0.599
4.149ArgGlu: 4.149 ± 0.699
2.108ArgPhe: 2.108 ± 0.515
5.796ArgGly: 5.796 ± 0.779
1.449ArgHis: 1.449 ± 0.331
2.898ArgIle: 2.898 ± 0.442
4.084ArgLys: 4.084 ± 0.547
6.059ArgLeu: 6.059 ± 0.731
2.173ArgMet: 2.173 ± 0.427
2.173ArgAsn: 2.173 ± 0.385
3.096ArgPro: 3.096 ± 0.441
3.359ArgGln: 3.359 ± 0.498
5.533ArgArg: 5.533 ± 0.664
4.084ArgSer: 4.084 ± 0.574
4.676ArgThr: 4.676 ± 0.463
4.61ArgVal: 4.61 ± 0.767
2.371ArgTrp: 2.371 ± 0.435
2.239ArgTyr: 2.239 ± 0.377
0.0ArgXaa: 0.0 ± 0.0
Ser
5.137SerAla: 5.137 ± 0.768
0.395SerCys: 0.395 ± 0.184
3.886SerAsp: 3.886 ± 0.62
2.898SerGlu: 2.898 ± 0.485
1.12SerPhe: 1.12 ± 0.266
5.533SerGly: 5.533 ± 0.699
0.988SerHis: 0.988 ± 0.214
2.569SerIle: 2.569 ± 0.436
2.766SerLys: 2.766 ± 0.455
4.808SerLeu: 4.808 ± 0.566
2.042SerMet: 2.042 ± 0.37
1.647SerAsn: 1.647 ± 0.316
2.7SerPro: 2.7 ± 0.397
2.569SerGln: 2.569 ± 0.329
3.557SerArg: 3.557 ± 0.506
3.557SerSer: 3.557 ± 0.511
3.359SerThr: 3.359 ± 0.511
3.688SerVal: 3.688 ± 0.511
1.317SerTrp: 1.317 ± 0.276
1.581SerTyr: 1.581 ± 0.383
0.0SerXaa: 0.0 ± 0.0
Thr
5.928ThrAla: 5.928 ± 0.607
0.198ThrCys: 0.198 ± 0.095
3.557ThrAsp: 3.557 ± 0.539
1.778ThrGlu: 1.778 ± 0.261
1.712ThrPhe: 1.712 ± 0.284
6.586ThrGly: 6.586 ± 1.782
1.449ThrHis: 1.449 ± 0.334
3.754ThrIle: 3.754 ± 0.498
2.766ThrLys: 2.766 ± 0.42
4.61ThrLeu: 4.61 ± 0.605
1.515ThrMet: 1.515 ± 0.342
1.515ThrAsn: 1.515 ± 0.327
4.347ThrPro: 4.347 ± 0.597
1.449ThrGln: 1.449 ± 0.26
3.688ThrArg: 3.688 ± 0.534
4.084ThrSer: 4.084 ± 0.574
4.347ThrThr: 4.347 ± 0.67
4.347ThrVal: 4.347 ± 0.482
0.724ThrTrp: 0.724 ± 0.231
1.515ThrTyr: 1.515 ± 0.336
0.0ThrXaa: 0.0 ± 0.0
Val
6.916ValAla: 6.916 ± 0.541
0.461ValCys: 0.461 ± 0.186
4.61ValAsp: 4.61 ± 0.432
3.557ValGlu: 3.557 ± 0.557
2.437ValPhe: 2.437 ± 0.424
4.742ValGly: 4.742 ± 0.561
1.251ValHis: 1.251 ± 0.262
3.754ValIle: 3.754 ± 0.584
2.437ValLys: 2.437 ± 0.336
5.401ValLeu: 5.401 ± 0.592
1.712ValMet: 1.712 ± 0.337
2.7ValAsn: 2.7 ± 0.436
3.952ValPro: 3.952 ± 0.468
2.569ValGln: 2.569 ± 0.477
5.071ValArg: 5.071 ± 0.612
3.491ValSer: 3.491 ± 0.475
5.006ValThr: 5.006 ± 0.524
5.071ValVal: 5.071 ± 0.699
2.305ValTrp: 2.305 ± 0.381
1.383ValTyr: 1.383 ± 0.262
0.0ValXaa: 0.0 ± 0.0
Trp
2.173TrpAla: 2.173 ± 0.48
0.527TrpCys: 0.527 ± 0.205
2.042TrpAsp: 2.042 ± 0.321
1.186TrpGlu: 1.186 ± 0.259
0.922TrpPhe: 0.922 ± 0.222
1.251TrpGly: 1.251 ± 0.333
0.724TrpHis: 0.724 ± 0.203
0.395TrpIle: 0.395 ± 0.161
0.856TrpLys: 0.856 ± 0.222
1.712TrpLeu: 1.712 ± 0.358
0.724TrpMet: 0.724 ± 0.289
1.12TrpAsn: 1.12 ± 0.251
0.988TrpPro: 0.988 ± 0.259
0.79TrpGln: 0.79 ± 0.246
1.054TrpArg: 1.054 ± 0.25
1.515TrpSer: 1.515 ± 0.314
1.12TrpThr: 1.12 ± 0.279
2.173TrpVal: 2.173 ± 0.437
0.395TrpTrp: 0.395 ± 0.191
0.922TrpTyr: 0.922 ± 0.249
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.766TyrAla: 2.766 ± 0.312
0.066TyrCys: 0.066 ± 0.058
1.778TyrAsp: 1.778 ± 0.373
2.173TyrGlu: 2.173 ± 0.372
0.79TyrPhe: 0.79 ± 0.186
2.239TyrGly: 2.239 ± 0.371
0.724TyrHis: 0.724 ± 0.221
0.395TyrIle: 0.395 ± 0.148
0.856TyrLys: 0.856 ± 0.219
2.305TyrLeu: 2.305 ± 0.39
0.988TyrMet: 0.988 ± 0.277
0.659TyrAsn: 0.659 ± 0.223
1.515TyrPro: 1.515 ± 0.305
0.527TyrGln: 0.527 ± 0.18
2.239TyrArg: 2.239 ± 0.433
2.042TyrSer: 2.042 ± 0.376
1.712TyrThr: 1.712 ± 0.264
2.503TyrVal: 2.503 ± 0.336
0.856TyrTrp: 0.856 ± 0.287
0.724TyrTyr: 0.724 ± 0.206
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 75 proteins (15184 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski