Leishmania donovani

Taxonomy: cellular organisms; Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina; Trypanosomatida; Trypanosomatidae; Leishmaniinae; Leishmania; Leishmania; Leishmania donovani species complex

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7554 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S7X0D3|A0A3S7X0D3_LEIDO Methylmalonyl-CoA mutase OS=Leishmania donovani OX=5661 GN=CGC21_31315 PE=3 SV=1
MM1 pKa = 7.48SGLRR5 pKa = 11.84QLIISAFFLVMGFLFLILGCTVVRR29 pKa = 11.84KK30 pKa = 9.99RR31 pKa = 11.84NAWPLMSLALYY42 pKa = 10.01CFAPVPFFLCGRR54 pKa = 11.84GADD57 pKa = 3.61SDD59 pKa = 4.64DD60 pKa = 5.6FIDD63 pKa = 6.25FDD65 pKa = 5.76DD66 pKa = 4.84EE67 pKa = 4.34PLDD70 pKa = 3.86AFSTVGLFMGGVLLISGPGLAVVLYY95 pKa = 7.66HH96 pKa = 6.36TSVICGLALFFTLLSGVCFIAAGVSLTVADD126 pKa = 5.15GGDD129 pKa = 3.64GDD131 pKa = 5.32DD132 pKa = 5.38AADD135 pKa = 4.44GYY137 pKa = 11.82NFF139 pKa = 4.0

Molecular weight: 14.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A504XJV5|A0A504XJV5_LEIDO Uncharacterized protein OS=Leishmania donovani OX=5661 GN=CGC21_33590 PE=4 SV=1
MM1 pKa = 7.63AKK3 pKa = 10.06SKK5 pKa = 10.65NHH7 pKa = 5.76TNHH10 pKa = 5.7NQSSKK15 pKa = 7.51NHH17 pKa = 6.0RR18 pKa = 11.84NGIKK22 pKa = 10.52GPMPLHH28 pKa = 5.97LHH30 pKa = 4.98NSKK33 pKa = 10.54RR34 pKa = 11.84GSWLPALVNARR45 pKa = 11.84RR46 pKa = 11.84VRR48 pKa = 11.84KK49 pKa = 9.49HH50 pKa = 4.74NQKK53 pKa = 10.61AALKK57 pKa = 9.79KK58 pKa = 9.32RR59 pKa = 11.84RR60 pKa = 11.84EE61 pKa = 4.12RR62 pKa = 11.84IAAFYY67 pKa = 10.27RR68 pKa = 11.84FSSFKK73 pKa = 8.86MAKK76 pKa = 9.74SKK78 pKa = 10.68NHH80 pKa = 5.76TNHH83 pKa = 5.7NQSSKK88 pKa = 7.51NHH90 pKa = 6.0RR91 pKa = 11.84NGIKK95 pKa = 10.52GPMPLHH101 pKa = 5.97LHH103 pKa = 4.98NSKK106 pKa = 10.54RR107 pKa = 11.84GSWLPALVNARR118 pKa = 11.84RR119 pKa = 11.84VRR121 pKa = 11.84KK122 pKa = 9.49HH123 pKa = 4.74NQKK126 pKa = 10.61AALKK130 pKa = 9.79KK131 pKa = 9.32RR132 pKa = 11.84RR133 pKa = 11.84EE134 pKa = 4.05RR135 pKa = 11.84IAAFAAKK142 pKa = 9.96NN143 pKa = 3.41

Molecular weight: 16.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7554

0

7554

5270428

73

18996

697.7

75.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.205 ± 0.057

1.879 ± 0.019

4.876 ± 0.024

6.042 ± 0.051

2.948 ± 0.019

6.505 ± 0.028

2.663 ± 0.014

2.999 ± 0.022

3.325 ± 0.023

9.251 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.248 ± 0.011

2.619 ± 0.015

5.789 ± 0.039

4.093 ± 0.05

7.28 ± 0.028

8.656 ± 0.046

5.955 ± 0.031

7.208 ± 0.083

1.078 ± 0.009

2.384 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski