Nautilia profundicola (strain ATCC BAA-1463 / DSM 18972 / AmH)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Nautiliales; Nautiliaceae; Nautilia; Nautilia profundicola

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1730 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B9L754|B9L754_NAUPA NOL1/NOP2/sun family protein OS=Nautilia profundicola (strain ATCC BAA-1463 / DSM 18972 / AmH) OX=598659 GN=NAMH_0021 PE=3 SV=1
MM1 pKa = 6.92KK2 pKa = 10.07TSAKK6 pKa = 10.24VLLSGIAASMILIGCGGGGSSTPSVSGTVEE36 pKa = 3.37ASYY39 pKa = 11.35LKK41 pKa = 10.88GITVCVKK48 pKa = 9.8DD49 pKa = 3.8TSTCATTDD57 pKa = 3.34SNGVFTLNNVSVPVEE72 pKa = 4.13LEE74 pKa = 3.96LKK76 pKa = 10.22IGNSVLADD84 pKa = 3.74LNVSTNNYY92 pKa = 9.4KK93 pKa = 8.67ITPAVLAEE101 pKa = 4.17NNTTIASYY109 pKa = 10.25IGAMLHH115 pKa = 6.98GIAGCDD121 pKa = 3.52LASDD125 pKa = 3.93VCDD128 pKa = 4.75FSSITTVDD136 pKa = 3.1INTSTNLPIITEE148 pKa = 4.34LKK150 pKa = 9.92TILEE154 pKa = 4.5QNSSASISVTVNDD167 pKa = 3.1STMQISEE174 pKa = 4.39VNATLYY180 pKa = 9.7ATEE183 pKa = 4.38NPSMTGTTAYY193 pKa = 10.23SFNGAASAGDD203 pKa = 3.74YY204 pKa = 11.4ASFTYY209 pKa = 10.42NSEE212 pKa = 4.17NNTISYY218 pKa = 9.7QISGNVYY225 pKa = 9.23GNVSGTRR232 pKa = 11.84EE233 pKa = 3.76IEE235 pKa = 3.87NLYY238 pKa = 11.18GNVFFKK244 pKa = 10.88DD245 pKa = 3.65KK246 pKa = 11.19NDD248 pKa = 3.63DD249 pKa = 4.15DD250 pKa = 3.61IFYY253 pKa = 10.32FFSGSLGVAVIPVSDD268 pKa = 3.82TNTSFVVGLQQPDD281 pKa = 3.53KK282 pKa = 11.17NITAEE287 pKa = 4.03DD288 pKa = 3.78LNLIVNKK295 pKa = 9.6KK296 pKa = 8.92YY297 pKa = 11.15NYY299 pKa = 9.76IEE301 pKa = 4.55FDD303 pKa = 3.56TDD305 pKa = 2.87EE306 pKa = 5.03SIYY309 pKa = 10.78FSIIEE314 pKa = 4.11INSTNTADD322 pKa = 6.06LNGTWADD329 pKa = 3.96YY330 pKa = 11.3VDD332 pKa = 3.62QSYY335 pKa = 7.96GTWEE339 pKa = 4.2VNGSHH344 pKa = 7.17LDD346 pKa = 3.48VFDD349 pKa = 4.01ARR351 pKa = 11.84GGKK354 pKa = 8.41IANVIIRR361 pKa = 11.84AGTSRR366 pKa = 11.84AGIVVDD372 pKa = 4.05NVDD375 pKa = 3.11GGFGVGVEE383 pKa = 4.49AKK385 pKa = 10.5ALTDD389 pKa = 3.62ADD391 pKa = 4.17LKK393 pKa = 9.37GTYY396 pKa = 9.51YY397 pKa = 11.23YY398 pKa = 10.87NDD400 pKa = 3.42SGSDD404 pKa = 3.64YY405 pKa = 10.4EE406 pKa = 4.81CYY408 pKa = 9.97GTVTVEE414 pKa = 3.86GTTFKK419 pKa = 11.36YY420 pKa = 10.2KK421 pKa = 10.75DD422 pKa = 3.65EE423 pKa = 4.25WCSDD427 pKa = 3.51NDD429 pKa = 4.05PEE431 pKa = 4.89SGSGTLVLNPTIDD444 pKa = 3.79PDD446 pKa = 4.09QNASTDD452 pKa = 3.57NNITLNGLAQVKK464 pKa = 10.28DD465 pKa = 3.81EE466 pKa = 4.73NGNLTDD472 pKa = 4.0EE473 pKa = 4.95FVFLDD478 pKa = 4.38PDD480 pKa = 3.27SGYY483 pKa = 10.68YY484 pKa = 9.8ISVNIDD490 pKa = 3.31DD491 pKa = 5.12GEE493 pKa = 4.11LSIGSNKK500 pKa = 8.88PLKK503 pKa = 10.53

Molecular weight: 53.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B9L9I0|B9L9I0_NAUPA Histidine kinase OS=Nautilia profundicola (strain ATCC BAA-1463 / DSM 18972 / AmH) OX=598659 GN=NAMH_0885 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.07QPSKK9 pKa = 9.35IRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.09RR14 pKa = 11.84THH16 pKa = 6.14GFRR19 pKa = 11.84ARR21 pKa = 11.84LKK23 pKa = 8.44TKK25 pKa = 10.35NGRR28 pKa = 11.84KK29 pKa = 8.73VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.87GRR39 pKa = 11.84KK40 pKa = 8.66RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1730

0

1730

519740

37

1668

300.4

34.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.241 ± 0.07

0.809 ± 0.02

5.422 ± 0.047

7.658 ± 0.072

5.424 ± 0.059

5.696 ± 0.061

1.566 ± 0.019

9.541 ± 0.077

10.356 ± 0.086

9.458 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.329 ± 0.025

5.798 ± 0.07

3.243 ± 0.032

2.312 ± 0.033

3.151 ± 0.038

5.181 ± 0.042

4.57 ± 0.044

6.269 ± 0.053

0.716 ± 0.018

4.262 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski